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Validamycin treatment significantly inhibits the glycometabolism and chitin synthesis in the common cutworm,Spodoptera litura

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Insect Science 2022年第3期29卷 840-854页

作者： hai-zhong yu Qin Zhang Zhan-Jun Lu Ming-Jie Deng College of Life Sciences Gannan Normal UniversityGanzhouJiangxi ProvinceChina National Navel Orange Engineering Research Center GanzhouJiangxi ProvinceChina Analytical and Testing Center School of Pharmaceutical SciencesWenzhou Medical UniversityWenzhouZhejiang ProvinceChina

Validamycin,as a broadly applied antibiotic,has been used to control rice sheath blight ***,validamycin was considered as an insecticide to control agricultural *** into the mechanism of validamycin’s action on insects can provide molecular targets for the control of agricultural *** this study,a toxicological test analysis revealed that Spodoptera litura larval growth and development was significantly inhibited and the pupation rate was significantly reduced with the increase of the concentration of *** to the NMR-based metabolomic analysis,a total of 15 metabolites involved in glycolysis and tricarboxylic acid cycle(TCA)pathways were ***,trehalase activities,glucose and chitin contents were significantly downregulated,but the trehalose content was upregulated after exposure to *** transcription quantitative PCR analysis revealed that the expression level of genes involved in glycolysis,TCA and chitin synthesis were upregulated after treating with *** chitin staining also confirmed that chitin content was downregulated at 12 h after validamycin *** results indicated that validamycin worked via two different molecular mechanisms,one through inhibiting glycometabolism and the other by inhibiting chitin synthesis in *** information lays a theoretical foundation for further control of ***.

关键词： chitin synthesis glycometabolism ^(1)H-NMR metabonomics Spodoptera litura validamycin

Inhibition of trehalase affects the trehalose and chitin metabolism pathways in Diaphorina citri(Hemiptera:Psyllidae)

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Insect Science 2021年第3期28卷 718-734页

作者： hai-zhong yu yu-Ling Huang Zhan-Jun Lu Qin Zhang Hua-Nan Su Yi-Ming Du Long Yi Ba-Lian zhong Ci-Xiang Chen College of Life Sciences Gannan Normal UniversityGanzhouJiangxiChina National Navel Orange Engineering Research Center GanzhouJiangxiChina China-USA Citrus Huanglongbing Joint Laboratory A Joint Laboratory of The University of Florida and Gannan Normal UniversityGanzhouJiangxiChina Fruit Bureau of Ganzhou GanzhouJiangxiChina

The Asian citrus psyllid,Diaphorina citri is the principal vector of huanglongbing,which transmits Candidatus Liberibacter *** is a key enzyme involved in trehalose hydrolysis and plays an important role in insect growth and *** specific functions of this enzyme in *** have not been *** this study,three trehalase genes(DcTreJ-1,DcTrel-2,and DcTre2)were identified based on the *** genome *** analysis showed that DcTrel-1 and DcTrel-2 are related to soluble trehalase,whereas DcTre2 is associated with membrane-bound *** expression analysis indicated that DcTrel-1 and DcTrel-2 had the highest expression levels in the head and wing,respectively,and DcTre2 had high expression levels in the fat ***,DcTrel-1 and DcTrel-2 expression levels were induced by 20-hydroxyecdysone and juvenile hormone III,but DcTre2 was *** expression levels of DcTrel-1,DcTrel-2,and DcTre2 were significantly up-regulated,which resulted in high mortality after treatment with *** activities and glucose contents were downregulated,but the trehalose content increased after treatment with *** addition,the expression levels of chitin metabolism-related genes significantly decreased at 24 and 48 h after treatment with ***,silencing of DcTrel-1,DcTrel-2,and DcTre2 reduced the expression levels of chitin metabolism-related genes and led to a malformed phenotype of *** results indicate that *** trehalase plays an essential role in regulating chitin metabolism and provides a new target for control of ***.

关键词： chitin metabolism Diaphorina citric RNA interference trehalase validamycin

State and parameter identification of linearized water wave equation via adjoint method

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Science China(Information Sciences) 2024年第10期67卷 283-297页

作者： Yang yu Cheng-zhong XU hai-Long PEI Jinpeng yu School of Automation Qingdao University LAGEP Batiment CPE University Claude Bernard-Lyon 1 School of Automation Science and Engineering Key Laboratory of Autonomous Systems and Networked ControlMinistry of Education Guangdong Engineering Technology Research Center of Unmanned Aerial Vehicle SystemSouth China University of Technology

In this paper, we focus on the state and parameter identification problem of a hydrodynamical system. This system is modeled as a linearized water wave equation(LWWE), a hyperbolic state-space model coupled with a Laplace equation. We assume that the wave elevation at two distinct points is the only measurement of water waves. We show that the state and water depth can be reconstructed from this point measurement records. The identification problem is recast as an optimization problem over an infinite-dimensional space. We propose the adjoint method-based identification algorithm to generate an estimated state and water depth. We then performed a numerical simulation to show the effectiveness of our designed algorithm by comparing it with existing studies.

关键词： linearized water wave equation (LWWE) point observation state estimation parameter identification adjoint method numerical simulation

Integrated transcriptome sequencing and RNA interference reveals molecular changes in Diaphorina citri after exposure to validamycin

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Insect Science 2021年第6期28卷 1690-1707页

作者： hai-zhong yu Yan-Xin Xie Jie Wang Ying Wang Yi-Min Du He-Gui Wang Ba-Lian zhong Bo Zhu Xiu-Dao yu Zhan-Jun Lu College of Life Sciences Gannan Normal UniversityGanzhouJiangxi ProvinceChina National Navel Orange Engineering Research Center GanzhouJiangxi ProvinceChina College of Life Science Anhui Agricultural UniversityHefeiChina

Validamycin has been widely used as a specific competitive inhibitor of *** our previous research,validamycin significantly inhibited trehalase activity and chitin synthesis in Diaphorina citri,resulting in abnormal ***,the mechanism of validamycin's action on *** remains ***,using a comparative transcriptome analysis,464 differentially expressed genes(DEGs)in *** were identified after validamycin treatment.A Gene Ontology enrichment analysis revealed that these DEGs were mainly involved in“small molecule process”,“structural molecule ac-tivity”and“transition metal ion binding”.DEGs involved in chitin metabolism,cuticle synthesis and insecticide detoxification were validated by reverse transcription quantitative polymerase chain *** RNA interference of *** chitinase-like protein EN03 and *** cuticle protein 7 genes significantly affected *** ***,the recombinant chitinase-like protein EN03 exhibited a chitin-binding property,and an antimicrobial activity against Bacillus *** study provides a first insight into the molecular changes in *** after exposure to validamycin and identifies two effective RNA interference targets for *** control.

关键词： chitin-binding Diaphorina citric RNA interference transcriptome sequencing validamycin

Hot versus cold subduction initiation

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National Science Review 2024年第2期11卷 101-102页

作者： zhong-hai Li Key Laboratory of Computational Geodynamics College of Earth and Planetary SciencesUniversity of Chinese Academy of Sciences

In the framework of plate tectonics, subduction initiation(SI) indicates the birth of a plate convergent boundary, which is a key point of plate tectonics and Wilson's cycle; however, its dynamic process is not well c... 详细信息

关键词： DRIVEN CRUST

A Novel Approach for Rapid Evaluating Cavitation Erosion Resistance of Metallic Materials

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Acta Metallurgica Sinica(English Letters) 2024年第7期37卷 1231-1237页

作者： yu Li Zhenbo Qin Xiaoyang Du Da-hai Xia Zepeng Gao Yiwen Zhang zhong Wu Wenbin Hu Tianjin Key Laboratory of Composite and Functional Materials School of Materials Science and EngineeringTianjin UniversityTianjin300072China Key Laboratory of Advanced Ceramics and Machining Technology(Ministry of Education) Tianjin UniversityTianjin300072China Joint School of National University of Singapore and Tianjin University International Campus of Tianjin UniversityFuzhou350207China

For the evaluation of cavitation erosion resistance of metallic materials,it has always been a time-consuming technical *** paper innovatively proposed a parameter named cavitation hardening rate to characterize the plastic deformation and work hardening behavior of metallic materials during the cavitation process and then found a positive correlation between cavitation hardening rate and strain hardening ***,the relationship between cavitation volume loss and strain hardening index was obtained,and a simple and rapid evaluation method for cavitation erosion resistance was established.

关键词： Cavitation erosion Evaluation method Cavitation hardening rate Volume loss

Recent progress in self-propelled particles

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Journal of Hydrodynamics 2024年第1期36卷 61-77页

作者： Zhen-yu Ouyang Jian-zhong Lin Laboratory of Impact and Safety Engineering Ministry of EducationNingbo UniversityNingbo315201China Department of Mechanics State Key Laboratory of Fluid Power and Mechatronic SystemsZhejiang UniversityHangzhou310027China

Self-propelled particles are commonly found in a large number of planktonic organisms such as bacteria,fungi,and algae in nature,and researchers have taken a long interest in exploring their swimming mechanisms for more than a *** in the past 20 years,with the development of computational fluid dynamics and flow display technology,as well as the need for the design of synthetic self-propelled particles and micro-swimming devices,self-propelled particles have become the forefront and hotspot of current research in the field of fluid *** paper first introduces the swimming characteristics of common self-propelled particles,leading to a classic“squirmer”type self-propelled particle *** this basis,a systematic introduction and summary of the theoretical and numerical simulation research of“squirmer”will be ***,the main challenges and opportunities faced by the current research will be summarized.

关键词： Self-propelled particles multiphase flow hydrodynamic interaction

High-performance Si/VO_(2)-nanorod heterojunction photodetector based on photothermoelectric effect for detecting human radiation

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Rare Metals 2024年第3期43卷 1177-1185页

作者： Fu-hai Guo Lan-zhong Hao Wei-Zhuo yu Si-Qi Li Guan-Chu Liu Jing-Yi Hao yun-Jie Liu College of Science China University of PetroleumQingdao266580China School of Materials Science and Engineering China University of PetroleumQingdao266580China

As a typical candidate of optoelectronic materials,vanadium dioxide(VO_(2))has wide applications in photodetectors(PDs),but is still challenging in largely enhancing the photodetecting performance for low-power human ***,high-performance Si/VO_(2)nanorods(NRs)heterojunction PDs based on the photothermoelectric(PTE)effect are *** uniform VO_(2)-NRs array films were deposited on Si by using magnetron sputtering technique,and a Si/VO_(2)heterojunctions were *** device exhibits a four-stage photoresponse and broadband photoresponse from ultraviolet to long-wavelength *** from the unique nanorods structure and the strong PTE effect,the fabricated device exhibits a large enhancement of the photodetecting performance,showing an ultrahigh photodetectivity of 1.6×10^(13)Jones and ultrafast response rates with a risingedge time of~65.0μs,three orders of magnitude higher than other VO_(2)-based ***,the device exhibits unique abilities to detect human radiation even when the human fingers are far away from the device surface up to 10.0 ***,the fabricated Si/VO_(2)devices can also be applied as breath sensors to distinguish different breathing *** results supply an effective route to design high-performance photodetectors toward detecting human thermal radiation and respiration.

关键词： Vanadium dioxide Heterojunction Photodetector Photothermoelectric Human radiation and respiration sensing

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Phase behavior of gas condensate in porous media using real-time computed tomography scanning

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Petroleum Science 2024年第2期21卷 1032-1043页

作者： Wen-Long Jing Lei Zhang Ai-Fen Li Jun-Jie zhong hai Sun Yong-Fei Yang yu-Long Cheng Jun Yao Institute of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongChina National Key Laboratory of Deep Oil and Gas China University of Petroleum(East China)Qingdao266580ShandongChina

The phase behavior of gas condensate in reservoir formations differs from that in pressure-volume-temperature(PVT)cells because it is influenced by porous media in the reservoir *** was used as a sample to investigate the influence of porous media on the phase behavior of the gas *** pore structure was first analyzed using computed tomography(CT)scanning,digital core technology,and a pore network *** sandstone core sample was then saturated with gas condensate for the pressure depletion *** each pressure-depletion state was stable,realtime CT scanning was performed on the *** scanning results of the sample were reconstructed into three-dimensional grayscale images,and the gas condensate and condensate liquid were segmented based on gray value discrepancy to dynamically characterize the phase behavior of the gas condensate in porous *** network models of the condensate liquid ganglia under different pressures were built to calculate the characteristic parameters,including the average radius,coordination number,and tortuosity,and to analyze the changing mechanism caused by the phase behavior change of the gas *** types of condensate liquid(clustered,branched,membranous,and droplet ganglia)were then classified by shape factor and Euler number to investigate their morphological changes dynamically and *** results show that the dew point pressure of the gas condensate in porous media is 12.7 MPa,which is 0.7 MPa higher than 12.0 MPa in PVT *** average radius,volume,and coordination number of the condensate liquid ganglia increased when the system pressure was between the dew point pressure(12.7 MPa)and the pressure for the maximum liquid dropout,Pmax(10.0 MPa),and decreased when it was below *** volume proportion of clustered ganglia was the highest,followed by branched,membranous,and droplet *** study provides crucial experimental evidence for the phase behavior chan

关键词： Gas condensate Pressure depletion Real-time micro-computed tomography scanning Distribution of condensate liquid

维普期刊数据库