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Adsorption Site and State of Nitrogen Atom on Ru(0001),(1010) Low-index and (1120),(1121) Stepped Surfaces

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Chemical Research in Chinese Universities 2009年第5期25卷 705-710页

作者： DIAO Zhao-yu yu hai-yan wang Zhong-ni ZHANG Xue-na wang Ze-xin Department of Chemistry Shandong Normal University Jinan 250014 P. R. China

The adsorptions of nitrogen atoms on Ru（0001）, （1010） low index surfaces and （1120）, （1121） stepped surfaces were investigated by the five-parameter Morse potential（5-MP） method in details, Calculated results demonstrate that N atoms show a tendency to be adsorbed at threefold sites. No subsurface state was found for N atoms on Ru（1010） surface. There exist 6 stable adsorption sites for N atoms on Ru（1121） stepped surface which can be classified into 3 types： the on-surface adsorption state, the facet adsorption state and the subsurface state. Moreover, two new perpendicular vibrations at 97 and 98 meV are predicted. All calculated results are in good accord with HR-EELS experiments.

关键词： N-Ru system 5-MP Surface adsorption

A New Voltammetric Enzyme Immunoassay System for the Detection of Alkaline Phosphatase

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Chinese Chemical Letters 2002年第1期13卷 69-70页

作者： Kui JIAO Wei SUN hai yu wang Department of Applied Chemistry Qingdao Institute of Chemical TechnologyQingdao 266042

A new voltammetric enzyme immunoassay system was investigated based on p-nitrophenyl phosphate (PNPP) as the substrate for alkaline phosphatase (ALP). PNPP is enzymatically hydrolyzed and the product p-nitrophenol (PNP) is detected by differential pulse voltammetry (DPV), which can be oxidized at +1.02 V (vs. Ag/AgCl) on bare glass carbon electrode (GCE). The conditions for enzymatic reaction and electrochemical detection were studied. According to this method, ALP can be detected with a detection limit of 2.8102 mU/L and a linear range of 4.0102 ~ 1.0106 mU/L.

关键词： Alkaline phosphatase p-nitrophenyl phosphate p-nitrophenol differential pulse voltammetry voltammetric enzyme immunoassay.

Bioinformatics-based Identification of Key Pathways and Hub Genes of Traumatic Brain Injury in a Rat Model

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Current Medical Science 2021年第3期41卷 610-617页

作者： Xin-yi CAO Xiao QIAN Guo-dong LIU yu-hai wang Wuxi Clinical College of Anhui Medical University(904th Hospital of the Joint Logistic Support Force of the Chinese People's Liberation Army) Wuxi 214000China

Traumatic brain injury(TBI)is a common injury caused by external forces that lead to damaged brain function or pathological changes in the brain *** explore the molecular mechanism and the hub genes of TBI,we downloaded gene expression profiles of the TBI model of rat and the sham control for the subsequent gene set enrichment analysis,pathway analysis and protein-protein interactions *** results of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that multiple biological pathways,including immune response,inflammatory response and cellular response to interleukin-1,as well as signaling pathways,such as tumor necrosis factor signaling pathway,chcmokine signaling pathway,cytokine-cytokine receptor interaction,Toll-like receptor signaling pathway and nuclear factor kappa B signaling pathway were implicated in the *** conclusion,this study provides insights into the molecular mechanism of TBI by screening the differentially expressed genes and hub genes that can be used as biomarkers and therapeutic targets.

关键词： traumatic brain injury bioinformatics analysis differentially expressed gene hub gene signal pathway

The latest research progress on minimally invasive treatments for hepatocellular carcinoma

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维普期刊数据库

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Hepatobiliary & Pancreatic Diseases International 2023年第1期22卷 54-63页

作者： Ya-Wen Zou Zhi-Gang Ren Ying Sun Zhen-Guo Liu Xiao-Bo Hu hai-yu wang Zu-Jiang yu Department of Infectious Diseases the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Key Laboratory of Pulsed Power Translational Medicine of Zhejiang Province 2959 Yuhangtang RoadHangzhou 310000China Gene Hospital of Henan Province/Precision Medicine Center the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China

Background:Hepatocellular carcinoma(HCC)is the fourth leading cause of cancer-related death *** to the high prevalence of hepatitis B virus(HBV)infection in China,the incidence of HCC in China is high,and liver cirrhosis caused by chronic hepatitis also brings great challenges to *** paper reviewed the latest research progress on minimally invasive treatments for HCC,including percutaneous thermal ablation and new nonthermal ablation techniques,and introduced the principles,advantages,and clinical applications of various therapeutic methods in *** sources:The data of treatments for HCC were systematically collected from the Pub Med,Science Direct,American Chemical Society and Web of Science databases published in English,using“minimally invasive”and“hepatocellular carcinoma”or“liver cancer”as the ***:Percutaneous thermal ablation is still a first-line strategy for the minimally invasive treatment of *** effect of microwave ablation(MWA)on downgrading treatment before liver transplantation is better than that of radiofrequency ablation(RFA),while RFA is more widely used in the clinical ***-intensity focused ultrasound(HIFU)is mainly used for the palliative treatment of advanced liver ***(ECT)delivers chemotherapeutic drugs to the target cells while reducing the blood supply around *** electroporation(IRE)uses a microsecond-pulsed electric field that induces apoptosis and necrosis and triggers a systemic immune *** nanosecond pulsed electric field(ns PEF)has achieved a good response in the ablation of mice with HCC,but it has not been reported in China for the treatment of human ***:A variety of minimally invasive treatments provide a sufficient survival advantage for HCC *** ablation will lead to a new wave with its unique advantage of antitumor recurrence and metastasis.

关键词： Hepatocellular carcinoma Radiofrequency ablation Minimally invasive Nanosecond pulsed electric field

Expression signatures of long non-coding RNA and mRNA in human traumatic brain injury

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Neural Regeneration Research 2019年第4期14卷 632-641页

作者： Li-Xiang Yang Li-Kun Yang Jie Zhu Jun-Hui Chen yu-hai wang Kun Xiong Department of Neurosurgery 101st Hospital of People's Liberation Army Department of Anatomy and Neurobiology School of Basic Medical Sciences Central South University

Long non-coding RNAs(lncRNAs) play a key role in craniocerebral disease, although their expression profiles in human traumatic brain injury are still unclear. In this regard, in this study, we examined brain injury tissue from three patients of the 101 st Hospital of the People's Liberation Army, China(specifically, a 36-year-old male, a 52-year-old female, and a 49-year-old female), who were diagnosed with traumatic brain injury and underwent brain contusion removal surgery. Tissue surrounding the brain contusion in the three patients was used as control tissue to observe expression characteristics of lncRNAs and mRNAs in human traumatic brain injury tissue. Volcano plot filtering identified 99 lncRNAs and 63 mRNAs differentially expressed in frontotemporal tissue of the two groups(P 1.2). Microarray analysis showed that 43 lncRNAs were up-regulated and 56 lncRNAs were down-regulated. Meanwhile, 59 mRNAs were up-regulated and 4 mRNAs were down-regulated. Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) analyses revealed 27 signaling pathways associated with target genes and, in particular, legionellosis and influenza A signaling pathways. Subsequently, a lncRNA-gene network was generated, which showed an absolute correlation coefficient value > 0.99 for 12 lncRNA-mRNA pairs. Finally, quantitative real-time polymerase chain reaction confirmed different expression of the five most up-regulated mRNAs within the two groups, which was consistent with the microarray results. In summary, our results show that expression profiles of mRNAs and lncRNAs are significantly different between human traumatic brain injury tissue and surrounding tissue, providing novel insight regarding lncRNAs' involvement in human traumatic brain injury. All participants provided informed consent. This research was registered in the Chinese Clinical Trial Registry(registration number: ChiCTR-TCC-13004002) and the protocol version number is 1.0.

关键词： nerve regeneration human traumatic brain injuries long noncoding RNA messenger RNA GO analysis real-time quantitative polymerase chain reaction biomarkers microarray analysis biological processes medical informatics neural regeneration

Characterization of oral and gut microbiome and plasma metabolomics in COVID-19 patients after 1-year follow-up

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Military Medical Research 2023年第2期10卷 162-174页

作者： Guang-Ying Cui Ben-Chen Rao Zhao-hai Zeng Xue-Mei wang Tong Ren hai-yu wang Hong Luo Hong-Yan Ren Chao Liu Su-Ying Ding Jun-Jie Tan Zhen-Guo Liu Ya-Wen Zou Zhi-Gang Ren Zu-Jiang yu Department of Infectious Diseases the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Gene Hospital of Henan Province/Precision Medicine Center the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Department of Infectious Diseases Guangshan County People’s HospitalGuangshan CountyXinyang 465450HenanChina Department of Breast Surgery Affiliated Cancer Hospital of Zhengzhou UniversityZhengzhou 450052China Shanghai Mobio Biomedical Technology Co. LtdShanghai 201111China Health Management Center the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China

Background:Due to the outbreak and rapid spread of coronavirus disease 2019(COVID-19),more than 160 million patients have become convalescents worldwide to *** alterations have occurred in the gut and oral microbiome and metabonomics of patients with ***,it is unknown whether their characteristics return to normal after the 1-year ***:We recruited 35 confirmed patients to provide specimens at discharge and 1 year later,as well as 160healthy controls.A total of 497 samples were prospectively collected,including 219 tongue-coating,129 stool and 149 plasma ***-coating and stool samples were subjected to 16S rRNA sequencing,and plasma samples were subjected to untargeted metabolomics ***:The oral and gut microbiome and metabolomics characteristics of the 1-year convalescents were restored to a large extent but did not completely return to *** the recovery process,the microbial diversity gradually *** acid-producing microbes and Bifidobacterium gradually increased,whereas lipopolysaccharideproducing microbes gradually *** addition,sphingosine-1-phosphate,which is closely related to the inflammatory factor storm of COVID-19,increased significantly during the recovery ***,the predictive models established based on the microbiome and metabolites of patients at the time of discharge reached high efficacy in predicting their neutralizing antibody levels one year ***:This study is the first to characterize the oral and gut microbiome and metabonomics in 1-year convalescents of *** key microbiome and metabolites in the process of recovery were identified,and provided new treatment ideas for accelerating *** the predictive models based on the microbiome and metabolomics afford new insights for predicting the recovery situation which benefited affected individuals and healthcare.

关键词： Coronavirus disease 2019(COVID-19) Gut microbiome Oral microbiome Plasma metabonomics Convalescents Individual outcomes Patient stratifcation Predictive Preventive and personalized medicine(3PM)

Irreversible electroporation plus chemotherapy versus chemotherapy alone as treatments for patients with locally advanced pancreatic cancer

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Hepatobiliary & Pancreatic Diseases International 2022年第1期21卷 69-72页

作者： Shan-Shuo Liu hai-yu wang Ying Sun Ya-Wen Zou Zhi-Gang Ren Xin-Hua Chen Zu-Jiang yu Department of Infectious Diseases The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Gene Hospital of Henan Province Precision Medicine CenterThe First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalZhejiang University School of MedicineKey Laboratory of Combined Multi-organ TransplantationMinistry of Public HealthHangzhou 310003China

Pancreatic cancer is a major health problem worldwide,with a high incidence and mortality *** is estimated that it has become the sixth leading cause of cancer-related death in China and the fourth leading cause of cancer-related death globally[1].For patients with locally advanced pancreatic cancer(LAPC),there are no standard treatment options.

关键词： patients chemotherapy mortality

Cyclophilin D-induced mitochondrial impairment confers axonal injury after intracerebral hemorrhage in mice

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Neural Regeneration Research 2023年第4期18卷 849-855页

作者： Yang Yang Kai-yuan Zhang Xue-Zhu Chen Chuan-Yan Yang Ju wang Xue-Jiao Lei yu-Lian Quan Wei-Xiang Chen Heng-Li Zhao Li-Kun Yang yu-hai wang yu-Jie Chen Hua Feng Department of Neurosurgery The 904th Hospital of PLAAnhui Medical UniversityWuxiJiangsu ProvinceChina Department of Neurosurgery and State Key Laboratory of Trauma Burn and Combined InjurySouthwest HospitalThird Military Medical University(Army Medical University)ChongqingChina Chongqing Key Laboratory of Precision Neuromedicine and Neuroregenaration Third Military Medical University(Army Medical University)ChongqingChina Wuxi Translational Medicine Center WuxiJiangsu ProvinceChina Department of Neurosurgery General Hospital of Xinjiang Military Command of PLAUrumqiXinjiang Uygur Autonomous RegionChina Department of Neurology The Second Medical Center&National Clinical Research Center for Geriatric DiseasesChinese PLA General HospitalBeijingChina

The mitochondrial permeability transition pore is a nonspecific transmembrane *** of mitochondrial permeability transition pore opening has been shown to alleviate mitochondrial swelling,calcium overload,and axonal *** D is an important component of the mitochondrial permeability transition *** cyclophilin D participates in mitochondrial impairment and axonal injury after intracerebral hemorrhage is not *** this study,we established mouse models of intracerebral hemorrhage in vivo by injection of autologous blood and oxyhemoglobin into the striatum in Thy1-YFP mice,in which pyramidal neurons and axons express yellow fluorescent *** also simulated intracerebral hemorrhage in vitro in PC12 cells using *** found that axonal degeneration in the early stage of intracerebral hemorrhage depended on mitochondrial swelling induced by cyclophilin D activation and mitochondrial permeability transition pore *** further investigated the mechanism underlying the role of cyclophilin D in mouse models and PC12 cell models of intracerebral *** found that both cyclosporin A inhibition and short hairpin RNA interference of cyclophilin D reduced mitochondrial permeability transition pore opening and mitochondrial *** addition,inhibition of cyclophilin D and mitochondrial permeability transition pore opening protected corticospinal tract integrity and alleviated motor dysfunction caused by intracerebral *** findings suggest that cyclophilin D is used as a key mediator of axonal degeneration after intracerebral hemorrhage;inhibition of cyclophilin D expression can protect mitochondrial structure and function and further alleviate corticospinal tract injury and motor dysfunction after intracerebral *** findings provide a therapeutic target for preventing axonal degeneration of white matter injury and subsequent functional impairment in central nervous diseases.

关键词： axonal injury corticospinal tract cyclophilin D cyclosporin A intracerebral hemorrhage mitochondrial impairment mitochondrial permeability transition pore motor dysfunction retraction bulb white matter

Effects of ulinastatin therapy in deep vein thrombosis prevention after brain tumor surgery:A single-center randomized controlled trial

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World Journal of Clinical Cases 2023年第31期11卷 7583-7592页

作者： yun-Na Tao Qian Han Wei Jiao Li-Kun Yang Fang wang Shan Xue Meng Shen yu-hai wang Department of Neurosurgery The 904th Hospital of Joint Logistic Support ForceWuxi 214044Jiangsu ProvinceChina Nursing The 904th Hospital of Joint Logistic Support ForceWuxi 214044Jiangsu ProvinceChina

BACKGROUND Venous thromboembolism(VTE)is a common neurosurgical complication after brain tumor resection,and its prophylaxis has been widely *** are no effective drugs in the clinical management of venous thromboembolism,and there is an absence of evidence-based medicine concerning the treatment of severe multiple *** To explore whether ulinastatin(UTI)can prevent VTE after brain tumor *** The present research included patients who underwent brain tumor *** received UTIs(400,000 IU)or placebos utilizing computer-based random sequencing(in a 1:1 ratio).The primary outcome measures were the incidence of VTE,coagulation function,pulmonary emboli,liver function,renal function,and drug-related adverse *** A total of 405 patients were evaluated between January 2019 and December 2021,and 361 of these were initially enrolled in the study to form intention-to-treat,which was given UTI(n=180)or placebo(n=181)treatment in a random *** were no statistically significant differences in baseline clinical data between the two *** incidence of VTE in the UTI group was remarkably improved compared with that in the placebo *** can improve coagulation dysfunction,pulmonary emboli,liver function,and renal *** significant difference was identified between the two groups in the side effects of UTI-induced diarrhea,vomiting,hospital stays,or hospitalization *** incidence of allergies was higher in the UTI group than in the placebo *** The findings from the present research indicated that UTI can decrease the incidence of VTE and clinical outcomes of patients after brain tumor resection and has fewer adverse reactions.

关键词： Ulinastatin Venous thromboembolism Brain tumor resection Randomized control trial Outcome