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Caseinolytic mitochondrial matrix peptidase X is essential for homologous chromosome synapsis and recombination during meiosis of male mouse germ cells

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Asian Journal of Andrology 2024年第2期26卷 165-174页

作者： hai-wei feng Yu Zhao Yan-Ling Gao Dong-Teng Liu Li-Jun Huo Key Laboratory of Agricultural Animal Genetics Breeding and ReproductionEducation Ministry of ChinaCollege of Animal Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China Shenzhen Key Laboratory of Fertility Regulation Reproductive Medicine CenterThe University of Hong Kong-Shenzhen HospitalShenzhen 518053China Maternal-Fetal Medicine Institute Department of Obstetrics and GynaecologyShenzhen Baoan Women's and Children's HospitalJinan UniversityShenzhen 518100China Department of Obstetrics and Gynaecology Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong SARChina

Meiosis is the process of producing haploid gametes through a series of complex chromosomal events and the coordinated action of various *** mitochondrial protease complex(ClpXP),which consists of caseinolytic mitochondrial matrix peptidase X(ClpX)and caseinolytic protease P(ClpP)and mediates the degradation of misfolded,damaged,and oxidized proteins,is essential for maintaining mitochondrial *** has been implicated in meiosis regulation,but its precise role is currently *** this study,we engineered an inducible male germ cell-specific knockout caseinolytic mitochondrial matrix peptidase X(Clpx^(cKO))mouse model to investigate the function of ClpX in *** found that disrupting Clpx in male mice induced germ cell apoptosis and led to an absence of sperm in the ***,it caused asynapsis of homologous chromosomes and impaired meiotic recombination,resulting in meiotic arrest in the zygotene-to-pachytene transition *** loss of ClpX compromised the double-strand break(DSB)repair machinery by markedly reducing the recruitment of DNA repair protein RAD51 homolog 1(RAD51)to DSB *** dysfunction may be due to an insufficient supply of energy from the aberrant mitochondria in Clpx^(cKO) spermatocytes,as discerned by electron ***,ubiquitination signals on chromosomes and the expression of oxidative phosphorylation subunits were both significantly attenuated in Clpx^(cKO) *** together,we propose that ClpX is essential for maintaining mitochondrial protein homeostasis and ensuring homologous chromosome pairing,synapsis,and recombination in spermatocytes during meiotic prophase I.

关键词： ClpX homologous chromosome meiosis mitochondrial recombination synapsis

Hydrogen inhalation promotes recovery of a patient in persistent vegetative state from intracerebral hemorrhage:A case report and literature review

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World Journal of Clinical Cases 2022年第4期10卷 1311-1319页

作者： Yan Huang feng-Ming Xiao Wen-Jie Tang Jing Qiao hai-feng wei Yuan-Yun Xie You-Zhen wei Department of Rehabilitation Qilu Children’s Hospital of Shandong UniversityJinan 250022Shandong ProvinceChina Research Center for Translational Medicine&Key Laboratory of Arrhythmias of the Ministry of Education of China Shanghai East HospitalTongji University School of MedicineShanghai 200120Shanghai ProvinceChina Department of Pediatrics Shanghai East HospitalTongji University School of MedicineShanghai 200120Shanghai ProvinceChina Department of Clinical Imaging The First Clinical Medical CollegeShandong University of Traditional Chinese MedicineJinan 250022Shandong ProvinceChina National Clinic and Medicine Research Institute for Geriatric Diseases Gannan Health Promotion and Translational LaboratoryThe First Affiliated HospitalGannan University of Medical SciencesGanzhou 341000Jiangxi ProvinceChina

BACKGROUND Persistent vegetative state(PVS)is a devastating and long-lasting clinical condition with high morbidity and mortality;currently,there are no available effective *** SUMMARY We report the case of an 11-year-old boy with PVS caused by severe intracerebral bleeding in the left hemisphere following anticoagulation *** patient’s PVS severity showed no notable improvement after 2-mo neuroprotective treatment and rehabilitation,including nerve growth factor and baclofen,hyperbaric oxygen,and comprehensive bedside rehabilitation *** inhalation treatment(4-6 h)of high-concentration hydrogen(H2)gas(66.6%H2+33.3%O2)was ***,the patient’s orientation,consciousness,ability to speak,facial expressions,and locomotor function were significantly restored,along with improvements in essential general health status,after H2 gas inhalation treatment,which was consistent with stabilized neuropathology in the left hemisphere and increased Hounsfield unit values of computed tomography in the right *** patient finally recovered to a near normal conscious state with a Coma Recovery Scale-Revised Score of 22 from his previous score of *** Phase 1 clinical trials are needed to explore the safety and efficacy of H2 gas inhalation in patients with PVS.

关键词： Hydrogen gas Intracerebral hemorrhage Consciousness recovery Persistent vegetative state Case report

Effect of Sodium Ferulate on Myocardial Ischemia Fibrosis Induced by Isoproterenol

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Chemical Research in Chinese Universities 2009年第5期25卷 699-704页

作者： GAO hai-cheng SUN Bo YU Xiao-yan GAO hai-mei SHI Yan MIAO Chun-sheng LI Xiang-jun LUO Ping ZOU Ying-gang wei hai-feng ZHAO Juan REN Li-qun School of Pharmacy Jilin University Changchun 130021 P. R. China The Second Clinical Hospital of Jilin University Changchun 130041 P. R. China

We examined whether the powerful sodium ferulate（SF） could improve myocardial ischemia fibrosis degree and gain the information of myocardial energy metabolism via experimental model of myocardial ischemia fibrosis. The model of myocardial ischemia fibrosis was made for the wistar rats induced with 15 mg/kg isoproterenol（Iso） subcutaneous injection. In experiment l, Iso effective model drug of myocardial ischemia fibrosis at present was administrated to the rats with myocardial ischemia fibrosis and 2, 4, 6, 12, 24, 48 and 72 h, 7 and 21 d later, the changes of adenosine content in rat myocardial tissue and hydroxyproline in blood serum were compared. In experiment 2, SF was administrated to the rats with myocardial ischemia fibrosis and 21 d later, the effect of SF on benazepril as reference drug curing myocardial ischemia fibrosis was evaluated by measuring the changes of adenosine in rat myocardial tissue and hydroxyproline content in blood serum. In the model group, there was a remarkable increase in hydroxyproline content and a decrease in adenosine content of myocardial tissue in experiment 1; there was a remarkable decrease in hydroxyproline in blood serum and a increase in adenosine content in myocardial tissue, which were recovered gradually to control in experiment 2（P〈0.05）. The results of experiment 1 show that with the increasing of pathological change degree, the rat adenosine content reduced gradually and arrived at the lowest value three weeks later（P〈0.05）; the hydroxyproline content was obviously higher compared with that of control group（P〈0.05）. The results of experiment 2 show that rat adenosine content in myocardial tissue and hydroxyproline content in blood serum were recovered gradually to normal level after injection SF. The effect of SF against myocardial ischemia fibrosis in preventive group is better than that of improving the myocardial ischemia fibrosis drug. Meanwhile, hinting HPLC is a good method to measure the adenosine content.

关键词： Energy metabolism Hydroxyproline Myocardial fibrosis HPLC

Altered intra- and inter-network brain functional connectivity in upper-limb amputees revealed through independent component analysis

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Neural Regeneration Research 2022年第12期17卷 2725-2729页

作者： Bing-Bo Bao Hong-Yi Zhu hai-feng wei Jing Li Zhi-Bin Wang Yue-Hua Li Xu-Yun Hua Mou-Xiong Zheng Xian-You Zheng Department of Orthopedic Surgery Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghaiChina Institute of Diagnostic and Interventional Radiology Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghaiChina Department of Traumatology and Orthopedics Yueyang HospitalShanghai University of Traditional Chinese MedicineShanghaiChina

Although cerebral neuroplasticity following amputation has been observed, little is understood about how network-level functional reorganization occurs in the brain following upper-limb amputation. The objective of this study was to analyze alterations in brain network functional connectivity(FC) in upper-limb amputees(ULAs). This observational study included 40 ULAs and 40 healthy control subjects;all participants underwent resting-state functional magnetic resonance imaging. Changes in intra-and inter-network FC in ULAs were quantified using independent component analysis and brain network FC analysis. We also analyzed the correlation between FC and clinical manifestations, such as pain. We identified 11 independent components using independent component analysis from all subjects. In ULAs, intra-network FC was decreased in the left precuneus(precuneus gyrus) within the dorsal attention network and left precentral(precentral gyrus) within the auditory network;but increased in the left Parietal_Inf(inferior parietal, but supramarginal and angular gyri) within the ventral sensorimotor network, right Cerebelum_Crus2(crus Ⅱ of cerebellum) and left Temporal_Mid(middle temporal gyrus) within the ventral attention network, and left Rolandic_Oper(rolandic operculum) within the auditory network. ULAs also showed decreased inter-network FCs between the dorsal sensorimotor network and ventral sensorimotor network, the dorsal sensorimotor network and right frontoparietal network, and the dorsal sensorimotor network and dorsal attention network. Correlation analyses revealed negative correlations between inter-network FC changes and residual limb pain and phantom limb pain scores, but positive correlations between inter-network FC changes and daily activity hours of stump limb. These results show that post-amputation plasticity in ULAs is not restricted to local remapping;rather, it also occurs at a network level across several cortical regions. This observation provides addit

关键词： amputation functional connectivity functional magnetic resonance imaging independent component analysis neuroimaging phantom pain phantom sensation resting-state networks

Influence of Medial Support Screws on the Maintenance of Fracture Reduction after Locked Plating of Proximal Humerus Fractures

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Chinese Medical Journal 2018年第15期131卷 1827-1833页

作者： Lang-Qing Zeng Lu-Lu Zeng Yu-Wen Jiang hai-feng wei Wen Zhang Yun-feng Chen Department of Orthopaedics Zhuhai City People's Hospital Jinan University Affiliated Zhuhai Hospital Jinan University Zhuhai Guangdong 519000 China Department of Anesthesiology Zhuhai City People's Hospital Jinan University Affiliated Zhuhai Hospital Jinan University Zhuhai Guangdong 519000 China Department of Orthopaedic Surgery Shanghai Sixth People's Hospital Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai 200233 China Lang-Qing Zeng and Lu-Lu Zeng contributed equally to the work.

Background： Technical aspects of the correct placement of medial support locking screws in the locking plate for proximal humerus fractures remain incompletely understood. This study was to evaluate the clinical relationship between the number of medial support screws and the maintenance of fracture reduction after locked plating of proximal humerus fractures. Methods： We retrospectively evaluated 181 patients who had been surgically treated for proximal humeral fractures （PHFs） with a locking plate between September 2007 and June 2013. All cases were then subdivided into one of four groups as follows： 75 patients in the medial cortical support （MCS） group, 26 patients in the medial multiscrew support （MMSS） group, 29 patients in the medial single screw support （MSSS） group, and 51 patients in the 11o medial support （NMS） group. Clinical and radiographic evaluations included the Constant-Murley score （CM）, visual analogue scale （VAS）, complications, and revision surgeries. The neck-shaft angle （NSA） was measured in a true anteroposterior radiograph immediately postoperation and at final follow-up. One-way analysis of variance or KruskaI-Wallis test was used for statistical analysis of measurement data, and Chi-square test or Fisher＇s exact test was used for categorical data. Results： The mean postoperative NSAs were 133.46°± 6.01°, 132.39° ± 7.77°. 135.17° ± 10.15°, and 132.41° ± 7.16° in the MCS, MMSS, MSSS, and NMS groups, respectively, and no significant differences were found （F = 1.02, P= 0.387）. In the final follow-up, the NSAs were 132.79° ±6.02°, 130.19° ± 9.25°, 131.28° ± 12.85°, and 127.35° ± 8.50° in the MCS, MMSS, MSSS, and NMS groups, respectively （F = 4.40, P = 0.008）. There were marked differences in the NSA at the final follow-up between the MCS and NMS groups （P = 0.004）. The median （interquartile range [IQR]） NSA losses were 0.0° （0.0-1.0）°, 1.3° （0.0-3.1）°, 1.5° （ 1.0-5.2）°, and 4.0° （ 1.2 -7.1 ）° in the MCS, MMSS, MSSS, and NMS groups, respective

关键词： Bone Plates Bone Screws Fracture Fixation, Internal Humeral Fractures, Proximal Postoperative Complications

Outcome of in vitro fertilization in endometriosis-associated infertility： a 5-year database cohort study

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Chinese Medical Journal 2012年第15期125卷 2688-2693页

作者： LIN Xiao-na wei Min-ling TONG Xiao-mei XU wei-hai ZHOU feng HUANG Qiong-xiao WEN Guo-feng ZHANG Song-ying Assisted Reproduction Unit Department of Obstetrics andGynecology Sir Run Run Shaw Hospital Zhejiang UniversitySchool of Medicine Hangzhou Zhejiang 310016 China

Background Endometriosis affects natural fertility through various approaches, and in vitro fertilization （IVF） is a good treatment. But the IVF result of endometriosis patients is still under debate. We investigated the effect of endometriosis on IVF by analyzing the data from a single reproductive center. Methods A retrospective, database-searched cohort study was performed. Relevant information was collected from the electronic records of women who underwent IVF/intracytoplasmic sperm injection between January 2006 and December 2010 in the Assisted Reproductive Unit of Sir Run Run Shaw Hospital. Patients with endometriosis were enrolled the study group. The rest of the women formed the control group. The main outcome was the clinical pregnancy rate. Secondary outcomes were oocytes retrieved number, fertilization rate, high-quality embryo rate, number of high-quality embryo for embryo transplantation, and implantation embryo/high-quality embryo ratio （IE/HQE ratio）. Comparisons were performed by the z2-test and independent t-test. Results The endometriosis group （n=177） had a markedly lower oocytes retrieved number, fertilization rate, implantation rate, and clinical pregnancy rate （7.6_＋5.1, 63.6%, 27.7%, and 45.2%, respectively） compared with the non-endometriosis group （n=4267; 11.8＋7.3, 68.4%, 36.2%, and 55.2%, respectively）. Stratified analysis showed that this difference was found in the subgroup younger than 35-years old, while only fertilization rate and implantation rate were different in the elder subgroup. The ratio of high-quality embryos transferred is lower in endometriosis group （53.7% vs. 71.8%, P 〈0.05）, but there is no difference in IE/HQE ratio between two groups. There is no significant difference in fertilization rate, implantation rate, and clinical pregnancy rate between mild and severe endometriosis patients. Conclusions Endometriosis patients suffer a decreasing IVF pregnancy rates mainly caused by reducing oocytes number and fertilization

关键词： fertilization in vitro, endometriosis pregnancy rate cohort study

Identification of CNS neural circuitry involved in the innervation of C7 spinal nerve： a viral transsynaptic tracing study

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Chinese Journal of Traumatology 2011年第5期14卷 259-263页

作者： wei hai-feng CHEN Liang GU Yu-dong Department of Orthopaedic Surgery Shanghai Sixth People＇s Hospital Shanghai Jiaotong University Shanghai 200233 China

Objective： Contralateral C7 spinal nerve transfer is a useful operation for the treatment of brachial plexus root avulsion. The recovery of the independent function at the ipsilateral side, however, depends on neural circuitry reorganization in the central nervous system （CNS）.This study tried to locate the CNS neuronal elements involved in the innervation ofC7 spinal ***： Pseudorabies virus （PRV, TK/gG-,2 μl）, which expressed green fluorescent protein （GFP）, was injected into the left C7 spinal nerve in 20 adult Sprague Dawley *** rats survived for 6 h, 12 h, 24 h and 36 h, the C1-C7segments of the spinal cord and brain were processed using a polyclonal immunohistochemical antibody against ***： PRV-labeled neurons were found mainly in gray matter of the C1-C7 segments of the spinal cord and at the following structures of the brain： lateral vestibular nucleus, lateral paragigantocellular nucleus, A5 cells, red nucleus, primary and secondary motor cortexes, primary and secondary somatosensory cortexes. Although located bilaterally, the PRV-labeled neurons existed predominantly in the ipsilateral side of the spinal cord and the contralateral side of the brain at 6-12 h after injection （p.i.）. The number of PRV-labeled neurons in the CNS was increasing with rat＇s survival time and the distribution of these neurons turned bilateral with no obvious dominance to either side at 24 h and 36 h （p.i.）.Conclusion： By use of transsynaptic tracing technique with PRV, the anatomically connected set of neurons,which modulates the activity of C7 spinal nerve, is located successfully in the CNS.

关键词： Brachial plexus Innervation Spinal nerves Central nervous system

基于状态观测器的表贴式永磁同步电机谐波参数辨识

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控制理论与应用 2018年第7期35卷 988-993页

作者：魏海峰韦汉培张懿江苏科技大学电子信息学院江苏镇江212000 江苏开璇智能科技有限公司江苏苏州215000

建立了考虑转子永磁体磁链和定子电感谐波分量的表贴式永磁同步电机数学模型,对其进行简化分析处理.基于理论分析法,对三相反电势(counter electromagnetic force,EMF)测量值进行快速傅立叶变换(fast Fourier transformation,FFT)分析,... 详细信息

建立了考虑转子永磁体磁链和定子电感谐波分量的表贴式永磁同步电机数学模型,对其进行简化分析处理.基于理论分析法,对三相反电势(counter electromagnetic force,EMF)测量值进行快速傅立叶变换(fast Fourier transformation,FFT)分析,得到三相反电势谐波分量,用以计算dq轴上的转子磁链6次和12次谐波分量幅值;在此基础上,设计基于Lyapunov稳定性理论的状态观测器对dq轴上的定子自感和互感6次谐波分量幅值进行辨识.实验结果验证了本文谐波参数辨识算法的有效性和实用性.

关键词：永磁同步电机状态观测器谐波参数辨识 Lyapunov

考虑滑模抖振的永磁同步电机反电势双级观测器

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控制理论与应用 2017年第6期34卷 738-745页

作者：魏海峰韦汉培张懿江苏科技大学电子信息学院江苏镇江212000 苏州绿的谐波传动科技有限公司江苏苏州215000

针对传统反电势滑模观测器抖振造成的永磁同步电机转子位置检测、转速估算不准确问题,在传统初级反电势滑模观测基础上,提出一种新型反电势双级观测器.通过构造反电势状态观测方程,滤除初级滑模观测器输出反电势高频纹波分量,以获得更... 详细信息

针对传统反电势滑模观测器抖振造成的永磁同步电机转子位置检测、转速估算不准确问题,在传统初级反电势滑模观测基础上,提出一种新型反电势双级观测器.通过构造反电势状态观测方程,滤除初级滑模观测器输出反电势高频纹波分量,以获得更为平滑与准确的反电势最优值,采用Lyapunov稳定性理论证明收敛性.同时,考虑观测器增益系数以及低通截止频率的给定,根据实时转速反馈选取观测系数,使得观测系数跟随转速变化获取最优观测值,设计变截止频率降低处理器对低通滤波器相位补偿的计算.对比仿真和实验结果验证了改进双级观测器算法的有效性和实用性.

关键词：永磁同步电机滑模抖振双级观测器反电势最优