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Molecular cloning and expression under abiotic stresses and hormones of the ethylene response factor Ⅶ gene FmRAP2.12 from Fraxinus mandshurica

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Journal of Forestry Research 2019年第4期30卷 1289-1300页

作者： Nansong liang Lei Yu Chunhao Liu Ziqing Wang Xingtang Zhao Yaguang zhan State Key Laboratory of Tree Genetics and Breeding Northeast Forestry University26 Hexing RoadHarbin 150040HeilongjiangPeople's Republic of China Department of Forest Bioengineering College of Life SciencesNortheast Forestry University26 Hexing RoadHarbin 150040HeilongjiangPeople's Republic of China

RELATED TO AP2.12(RAP2.12)is one of the Ethylene Response Factors(ERF)transcription factor and plays a key role in controlling plant root bending and responding to multiple abiotic stresses including hypoxia *** this study,FmRAP2.12 gene was isolated and characterized from Fraxinus mandshurica *** open reading frame(ORF)of FmRAP2.12 was 1170 bp and encoded a protein of 389 amino *** conserved domains,three-dimensional phylogenetic relationship of FmRAP2.12 was also *** real-time(qRT-PCR)analyzed the expression of FmRAP2.12 in different *** expression level of FmRAP2.12 was highest in roots followed by leaves,and lowest in male *** stress and hormone signal-induced expression was established using *** stress induced FmRAP2.12 to a double peak pattern:the first peak value was at 6 h and the second at 72 *** stress also induced FmRAP2.12 to a double peak pattern:the first at6 h and the second at 48 ***2.12 was up-regulated after initiation of gibberellic acid(GA3)treatment,with a one peak pattern at 24 ***2.12 may not respond to cold stress and Abscisic acid(ABA)*** transient overexpression of FmRAP2.12 caused the up-expression of downstream key genes of abiotic stress response and gibberellin *** research reveals the molecular characteristic and expression patterns under abiotic stress and hormone condition of FmRAP2.12,providing support for the genetic improvement of *** at a molecular level.

关键词： ERF RAP2.12 Gene clone Gene expression Fraxinus mandshurica

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Isolation and Structural Indentification of Herbicidal Toxin Fractions Produced by Pythium aphanidermatum

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Agricultural Sciences in China 2010年第7期9卷 995-1000页

作者： zhanG Li-hui KANG zhan-hai XU Jiao XU Wen-chao zhanG Jin-lin College of Plant Protection Agricultural University of Hebei Baoding 071001 P.R. China

In order to understand the compsition and structure of herbicidal component of Pythium aphanidermatum,the isolation and structural indentification were *** culture filtrate was extracted by ethyl acetate,petroleum,and chloroform with the same volume respectively and the activity of the crude toxin was *** toxin was separated by using the method of thin layer chromatography（TLC）,then the main fraction was separated by HPLC,and the structure was analyzed by the sepctrum of IR,13C-NMR and *** results showed that the ethyl acetate extracts had the strongest herbicidal *** the method of TLC,the bioassay results showed that the extracts with Rf 0.19 had the strongest effect on weeds and the inhibition to Digitaria sanguinalis and Amaranthus retroflexus reached five levels,and the component was proved to be dimethyl o-phthalate from the spectrum of IR,13C-NMR and 1HNMR,which was one of the components from the toxin,and it had herbicidal activity.

关键词： Pythium aphanidermatum toxin herbicidal structure identification

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Estimating potential yield of wheat production in China based on cross-scale data-model fusion

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Frontiers of Earth Science 2012年第4期6卷 364-372页

作者： zhan TIAN Honglin ZHONG Runhe SHI Laixiang SUN Gunther FISCHER Zhuoran liang Key Laboratory of Geographic Information Science Ministry of Education East China Normal University Shanghai 200062 China Shanghai Climate Center Shanghai Meteorological Bureau Shanghai 200030 China International Institute for Applied System Analysis Laxenburg 2361 Austria

The response of the agro-ecological system to the environment includes the response of individual crop＇s physiologic process and the adaption of the crop commu- nity to the environment. Observation and simulation at the single scale level cannot fully explain the above process. It is necessary to develop cross-scale agro-ecological models and study the interaction of agro-ecological processes across different scales. In this research, two typical agro- ecological models, the Decision Support System for Agro- technology Transfer （DSSAT） model and the Agro- ecological Zone （AEZ） model, are employed, and a framework for effective cross-scale data-model fusion is proposed and illustrated. The national observed data from 36 different agricultural observation stations and historical weather stations （1962-1999） are employed to estimate average crop productivity. Comparison of the two models＇ estimations are consistent, which would indicate the possibility ofcross-scale crop model fusion.

关键词： DSSAT model AEZ model data-model fusion agro-ecological system

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Modeling and Analysis of Risk Propagation and Loss Causing Capacity for Key Nodes in Cyber-Physical Coupled Power Network

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Complex System Modeling and Simulation 2024年第2期4卷 124-136页

作者： Dongqi Liu Qiong zhang Haolan liang Tao zhan Rui Wang National Key Laboratory of Disaster Prevention and Reduction for Power Grid Changsha University of Science and TechnologyChangsha 410114China the School of Electrical and Information Engineering Changsha University of Science and TechnologyChangsha 410114China the College of Electrical and Information Engineering Hunan Institute of EngineeringXiangtan 411104China the School of Systems Engineering National University of Defense TechnologyChangsha 410003China

The modern power system has evolved into a cyber-physical system with deep coupling of physical and information domains,which brings new security *** at the problem that the“information-physical”cross-domain attacks with key nodes as springboards seriously threaten the safe and stable operation of power grids,a risk propagation model considering key nodes of power communication coupling networks is proposed to study the risk propagation characteristics of malicious attacks on key nodes and the impact on the ***,combined with the complex network theory,a topological model of the power communication coupling network is established,and the key nodes of the coupling network are screened out by Technique for Order Preference by Similarity to Ideal Solution(TOPSIS)method under the comprehensive evaluation index based on topological characteristics and physical ***,a risk propagation model is established for malicious attacks on key nodes to study its propagation characteristics and analyze the state changes of each node in the coupled ***,two loss-causing factors:the minimum load loss ratio and transmission delay factor are constructed to quantify the impact of risk propagation on the coupled ***,simulation analysis based on the IEEE 39-node system shows that the probability of node being breached(α)and the security tolerance of the system(β)are the key factors affecting the risk propagation characteristics of the coupled network,as well as the criticality of the node is positively correlated with the damage-causing *** proposed methodological model can provide an effective exploration of the diffusion of security risks in control systems on a macro level.

关键词： cyber-physical system risk propagation complex network loss causative factor

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Construction of Vesicles, Micro/Nanorods and Ultralong Nanotubes through the Self-Assembly of Non-Classical Amphiphiles with Rigid Conformation

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Chinese Journal of Chemistry 2017年第4期35卷 429-434页

作者： Feng Lin Rongran liang Qiaoyan Qi Tianguang zhan zhanting Li Xin Zhao Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 China Department of Chemistry Fudan University Shanghai 200433 China

Amphiphilic molecules have long been regarded as an important class of supramolecular building blocks for the fabrication of nanomaterials. While most previous researches have mainly focused on amphiphlies with flexible structures, in this work, four novel amphiphiles possessing wholly-rigid skeletons have been designed and synthe- sized. These molecules were built by using 4,4＇-bipyridin-l-ium or viologen as hydrophilic moieties and phenyl or biphenyl as hydrophobic segments, bridged by a pyridazine unit. Their self-assembly behavior has been investigated by scanning electron microscopy （SEM）, atomic force microscopy （AFM） and transmission electron microscopy （TEM）, which revealed they could self-assemble into well-ordered nanoarchitectures with various morphologies such as vesicles, micro/nanorods and nanotubes in water or methanol, depending on their hydrophilic/hydrophobic fraction ratios.

关键词： amphiphiles self-assembly hydrophobic-lipophilic interaction aromatic stacking rigid conformation

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Derivation of the Spiral Motion of an Eruptive Prominence and Its Explanation

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Chinese Journal of Astronomy and Astrophysics 2006年第5期6卷 617-624页

作者： Xiao-Ma Gu Shu-Hua Zhong Hong-Fei liang La-Sheng zhan National Astronomical Observatories / Yunnan Observatory Chinese Academy of Sciences Kunming650011 Jingdezhen Ceramic Institute Jingdezhen 333000

A 2D velocity field of the eruptive prominence （EP） of 1991 March 5 is obtained from its spectral data observed at the Yunnan Observatory and the velocity distributions along the entrance slit are derived for different observing frames. Under the assumption that matter in the EP undergoes axial, radial and possible rotational motions, we construct a theoretical velocity distribution of the EP along the entrance slit, to derive, by fitting, the angular velocity of rotation ω and the other three parameters （axial velocity v0, radial velocity vr and the angle between the EP plane and the line of sight Ф）. We found： an averaged angular velocity ω of 3.0 × 10^-3 arc s^-1 and the variation of ω with the height above the solar limb. As the EP rises, the matter within it in fact moves along a spiral path around its axis. The spiral motion may be explained by the theory of plasma ‘double pole diffusion＇（DPD） caused by a sharp density gradient between the eruptive prominence and the surrounding corona. A theoretical angular velocity ω′ is estimated based on the DPD and basically coincides with ω obtained from the optimal velocity fitting.

关键词： Sun eruptive prominence -- Sun Spiral motion

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Reducing LncRNA-5657 expression inhibits the brain inflammatory reaction in septic rats

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Neural Regeneration Research 2021年第7期16卷 1288-1293页

作者： Yi-An zhan Xin-liang Qiu Xu-Zhen Wang Ning Zhao Ke-Jian Qian Department of Critical Care Medicine the First Affiliated Hospital of Nanchang UniversityNanchangJiangxi ProvinceChina Department of Critical Care Medicine Xingguo County People’s HospitalGanzhouJiangxi ProvinceChina

Our preliminary study found that the long noncoding RNA(LncRNA)-5657 can reduce the expression of inflammatory factors during inflammatory reactions in rat glial ***, the role played by LncRNA-5657 during septic brain injury remains *** the present study, rat models of septic encephalopathy were established by cecal ligation and puncture, and then the rats were treated with a hippocampal injection small hairpin RNA(sh RNA) against LncRNA-5657(sh-LnCRNA-5657).The sh-LncRNA-5657 treatment reduced the level of neuronal degeneration and necrosis in the rat hippocampus, reduced the immunoreactivities of aquaporin 4, heparanase, and metallopeptidase-9, and lowered the level of tumor necrosis *** cells were pre-treated with sh-LncRNA-5657 and then treated with 1 μg/mL ***-LncRNA-5657 transfection decreased the expression of LncRNA-5657 in lipopolysaccharide-treated glial cells and decreased the mRNA and protein levels of tumor necrosis factor-alpha, interleukin-1β, and *** findings suggested that LncRNA-5657 expression can significantly reduce the inflammatory reaction during septic encephalopathy and induce protective effects against this *** study was approved by the Institutional Ethics Committee at the First Affiliated Hospital of Nanchang University of China(approval No.2017-004) in 2017.

关键词： brain injury glial cells inflammation injury lipopolysaccharide long noncoding RNA repair sepsis

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Focusing on the recent progress of tea polyphenol chemistry and perspectives

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Food Science and Human Wellness 2022年第3期11卷 437-444页

作者： Shiming Li liang zhang Xiaochun Wan Jianfeng zhan Chi-Tang Ho Hubei Key Laboratory of EFGIR Huanggang Normal UniversityHuanggang 438000China State Key Laboratory of Tea Plant Biology and Utilization School of Tea&Food ScienceAnhui Agricultural UniversityHefei 230036China Department of Food Science Rutgers UniversityNJ 08901USA

Myriad evidence attests to the health-promoting benefits of tea *** there are multiple factors of tea influencing the effective biological properties,tea polyphenols are the most significant and valuable *** chemical characterization and physical characteristics of tea polyphenols have been comprehensively studied over the previous *** the emergence of new chemistry in tea,particularly the property of scavenging reactive carbonyl species(RCS)and the newly discovered flavoalkaloid compounds,has drawn increasing *** this review,we summarize recent findings of a new class of compounds in tea-flavonoid alkaloids(flavoalkaloids),which exist in fresh tea leaves and can be generated during the process of post-harvesting,and also postulate the formation mechanism of flavoalkaloids between catechins and theanine-derived Strecker ***,we detail the up-to-date research results of tea polyphenols regarding their ability to trap RCS and their in vivo aminated metabolites to suppress advanced glycation ends products(AGEs).We further raise questions to be addressed in the near future,including the synthetic pathways for the generation of flavoalkaloids and AGEs in fresh tea leaves before processing and the concentrations of tea polyphenols that affect their RCS scavenging capability due to their pro-oxidant *** intensive research is warranted to elucidate the mechanisms of action underlying the biological activity of flavoalkaloids and the pharmacological application of tea polyphenols in scavenging RCS and impeding detrimental AGEs.

关键词： Tea chemistry Flavonoalkaloids RCS adducts Aminated tea polyphenols AGEs

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Self-Triggered Impulsive Control for Nonlinear Stochastic Systems

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IEEE/CAA Journal of Automatica Sinica 2025年第1期 264-266页

作者： Tao zhan Yi Ji Yabin Gao Hongyi Li Yuanqing Xia the School of Mathematics and Statistics Southwest University the School of Astronautics Harbin Institute of Technology IEEE the College of Electronic and Information Engineering Southwest University Zhongyuan University of Technology the School of Automation Beijing Institute of Technology

Dear Editor,This letter deals with the stabilization problem of nonlinear stochastic systems via self-triggered impulsive control(STIC), where the timing of impulsive control actions is not dependent on continuous state monitoring. In contrast to the existing self-triggered control method, novel self-triggered mechanism(STM) is proposed by incorporating a waiting time for stabilizing impulses. This allows for direct prediction of the next impulsive instant.

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A Locating Method for Reliability-Critical Gates with a Parallel-Structured Genetic Algorithm

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Journal of Computer Science & Technology 2019年第5期34卷 1136-1151页

作者： Jie Xiao zhan-Hui Shi Jian-Hui Jiang Xu-Hua Yang Yu-Jiao Huang hai-Gen Hu College of Computer Science and Technology Zhejiang University of TechnologyHangzhou 310023China School of Software Engineering Tongji UniversityShanghai 201804China

The reliability allowance of circuits tends to decrease with the increase of circuit integration and the application of new technology and materials, and the hardening strategy oriented toward gates is an effective technology for improving the circuit reliability of the current situations. Therefore, a parallel-structured genetic algorithm (GA), PGA, is proposed in this paper to locate reliability-critical gates to successfully perform targeted hardening. Firstly, we design a binary coding method for reliability-critical gates and build an ordered initial population consisting of dominant individuals to improve the quality of the initial population. Secondly, we construct an embedded parallel operation loop for directional crossover and directional mutation to compensate for the deficiency of the poor local search of the GA. Thirdly, for combination with a diversity protection strategy for the population, we design an elitism retention based selection method to boost the convergence speed and avoid being trapped by a local optimum. Finally, we present an ordered identification method oriented toward reliability-critical gates using a scoring mechanism to retain the potential optimal solutions in each round to improve the robustness of the proposed locating method. The simulation results on benchmark circuits show that the proposed method PGA is an efficient locating method for reliability-critical gates in terms of accuracy and convergence speed.

关键词： gate-level circuit reliability locating reliability-critical gate parallel-structured genetic algorithm directing strategy scoring mechanism

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