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C-reactive protein aggravates myocardial ischemia/reperfusion injury through activation of extracellular-signal-regulated kinase 1/2

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Journal of Geriatric Cardiology 2018年第7期15卷 502-513页

作者： Wei-Na PEI hai-juan hu Fan LIU Bing XIAO Ya-Bei ZUO Wei CUI Department of Cardiology Second Hospital of Hebei Medical University and Institute of Cardiocerebrovascular Disease of Hebei Province Shijiazhuang Hebei China

Background Ischemia/reperfusion injury （IRI） is an inflammatory response that occurs when tissue is reperfused following a prolonged period of ischemia. Several studies have indicated that C-reactive protein （CRP） might play an important role in inducing IRI. However, the effects of CRP on myocardial IRI and the underlying mechanisms have not been fully elucidated. This study aimed to investigate the association between CRP and myocardial IRI and the underlying mechanisms. Methods We simulated ischemia/reperfusion using oxygen-glucose deprivation/ reoxygenation （OGD/R） in neonatal Sprague-Dawley rat cardiomyocytes; reperfusion injury was induced by three hours of hypoxia with glucose and serum deprivation followed by one hour of reperfusion. Cell viability was tested with MTS assays, and cardiomyocyte damage was evaluated by lactate dehydrogenase （LDH） leakage. Mitochondrial membrane potential was measured using tetramethylrhodamine ethyl ester （TMRE） and mitochondrial permeability transition pore （mPTP） opening was measured using calcein/AM; both TMRE and caocein/AM were visualized with laser scanning confocal microscopy. In addition, we studied the signaling pathways underlying CRP-mediated ischemia/reperfusion injury via Western blot analysis. Results Compared with the simple OGD/R group, after intervention with 10 pg/mL CRP, cell viability decreased markedly （82.36 % ± 6.18% vs. 64.84% ± 4.06%, P = 0.0007）, and the LDH leakage significantly increased （145.3 U/L ± 16.06 U/L vs. 208.2 U/L ± 19.23 U/L, P = 0.0122）. CRP also activated mPTP opening and reduced mitochondrial membrane potential during myocardial ischemia/reperfusion. Pretreatment with 1 pM atorvastatin （Ator） before CRP intervention protected cardiomyocytes from IRI. Mitochondrial KATP channel opener diazoxide and mPTP inhibitor cyclosporin A also offset the effects of CRP in this process. The level of phosphorylated extracellular-signal-regulated kinase （ERK） 1/2 was significantly higher after pre-tre

关键词： C-reactive protein Ischemia/reperfusion injury Mitochondrial permeability transition pore Mitochondrial KATP channel Statin

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Integrin beta 3-overexpressing mesenchymal stromal cells display enhanced homing and can reduce atherosclerotic plaque

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World Journal of Stem Cells 2023年第9期15卷 931-946页

作者： hai-juan hu Xue-Ru Xiao Tong Li De-Min Liu Xue Geng Mei Han Wei Cui First Division Department of CardiologyThe Second Hospital of Hebei Medical University and Institute of Cardiocerebrovascular Disease of Hebei ProvinceShijiazhuang 050000Hebei ProvinceChina Department of Obstetrics Shijiazhuang People's HospitalShijiazhuang 050030Hebei ProvinceChina Key Laboratory of Medical Biotechnology of Hebei Province Department of Biochemistry and Molecular BiologyCollege of Basic MedicineCardiovascular Medical Science CenterHebei Medical UniversityShijiazhuang 050017Hebei ProvinceChina

BACKGROUND Umbilical cord(UC)mesenchymal stem cell(MSC)transplantation is a potential therapeutic intervention for atherosclerotic vascular *** beta 3(ITGB3)promotes cell migration in several cell ***,whether ITGBmodified MSCs can migrate to plaque sites in vivo and play an anti-atherosclerotic role remains *** To investigate whether ITGB3-overexpressing MSCs(MSCs^(ITGB3))would exhibit improved homing efficacy in *** UC MSCs were isolated and *** vectors encoding ITGB3 or green fluorescent protein(GFP)as control were transfected into *** male apolipoprotein E-/-mice were acquired from Beijing Vital River Lab Animal Technology Co.,Ltd and fed with a high-fat diet(HFD)for 12 wk to induce the formation of atherosclerotic *** HFD-fed mice were randomly separated into three ***-labeled MSCs(MSCs^(GFP))or MSCs^(ITGB3)were transplanted into the mice intravenously via the tail *** staining,Oil red O staining,histological analyses,western blotting,enzymelinked immunosorbent assay,and quantitative real-time polymerase chain reaction were used for the *** ITGB3 modified MSCs successfully differentiated into the“osteocyte”and“adipocyte”phenotypes and were characterized by positive expression(>91.3%)of CD29,CD73,and CD105 and negative expression(human Leukocyte *** a transwell assay,MSCs^(ITGB3)showed significantly faster migration than ***3 overexpression had no effects on MSC viability,differentiation,and *** staining revealed that ITGB3 overexpression substantially enhanced the homing of MSCs to plaque *** red O staining and histological analyses further confirmed the therapeutic effects of MSCs^(ITGB3),significantly reducing the plaque ***-linked immunosorbent assay and quantitative real-time polymerase chain reaction revealed that MSC^(ITGB3)transplantation considerably decr

关键词： Atherosclerosis Inflammation Integrin beta 3 Mesenchymal stem cells Arg-Gly-Asp structure Umbilical cord

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Livelihood changes and evolution of upland ethnic communities driven by tourism:a case study in Guizhou Province,southwest China

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Journal of Mountain Science 2016年第7期13卷 1313-1332页

作者： LI Ya-juan VV hu CHEN Tian hu Jing CUI hai-yang The College of Urban&Environmental Sciences Central China Normal UniversityWuhan 430079China Wuhan Branch of China Tourism Academy Wuhan 430079China Institute of Geographic Sciences and Natural Resources Research University of Chinese Academy of SciencesBeijing 100101China ASEAN(Association of Southeast Asian Nations)Research Center Guizhou UniversityGuiyang 550025China

As an effective livelihood approach to alleviate poverty without rural population migration, ethnic tourism has become the primary choice of economic development in ethnic areas worldwide in addition to traditional livelihood approaches. This article applies the theories of livelihood to study the community evolution driven by tourism livelihood and examine three mountainous tourism communities in different stages of tourist area life cycle. Drawing on the methods of GIS spatial analysis, semi-structured interviews and questionnaires, this article proposes a sustainable livelihood framework for ethnic tourism to explore the evolution of ethnic tourism communities by identifying changes in livelihood assets(natural, financial, social, cultural and human capitals) in the process of tourism development. The results show that the development of ethnic tourism has led to changes in the increase of building land, and the diversification of land use functions with a trend of shifting from meeting local villagers' living needs to satisfying tourists, income composition and uneven distribution of tourism income spatially. Ethnic tourism also led to the deterioration of traditional social management structure, collapse of neighboring relationship, the over- commercialization and staged authenticity of ethnic culture, as well as the gradual vanish of agricultural knowledge with a trend of increasing modern business knowledge and higher education. In addition, these changes, involving livelihood assets from natural, economic, human, social and cultural aspects are interrelated and interactive, which form new evolution characters of ethnic community. This study reveals the conflicts over livelihood approaches which have formed new vulnerabilities to impact on sustainable evolution of ethnic communities. This research provides implications for achieving the sustainable development of ethnic communities with the driving force of tourism livelihood.

关键词： Community evolution Tourism livelihood Livelihood assets Ethnic tourism Guizhou

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数字技术驱动下中国民歌变革及应对策略研究

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湖北科技学院学报 2025年第1期45卷 80-85页

作者：胡娟方元河南职业技术学院音乐学院河南郑州450046

数字科技的崛起对音乐创作和传播方式带来了革命性的变化,同时也对中国民歌的传承和发展提出了新的课题。本研究旨在深入探讨数字化发展对中国民歌的影响,包括作品表演形式与作品的传承与创新,并详细分析其所面临的挑战和机遇,提出相应... 详细信息

数字科技的崛起对音乐创作和传播方式带来了革命性的变化,同时也对中国民歌的传承和发展提出了新的课题。本研究旨在深入探讨数字化发展对中国民歌的影响,包括作品表演形式与作品的传承与创新,并详细分析其所面临的挑战和机遇,提出相应的对策和建议。通过理论支持和实践指导,期望为传承和创新中国民歌作出积极贡献。

关键词：数字技术中国民歌变革应对

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A review on photocatalytic systems capable of synchronously utilizing photogenerated electrons and holes

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Rare Metals 2022年第7期41卷 2387-2404页

作者： Li-juan Sun hai-Wei Su Qin-Qin Liu Jie hu Le-Le Wang hua Tang School of Materials Science and Engineering Jiangsu UniversityZhenjiang 212013China State Key Laboratory of Photocatalysis on Energy and Environment College of ChemistryFuzhou UniversityFuzhou 350108China School of Environmental Science and Engineering Qingdao UniversityQingdao 266071China

With the continuous progress and development of current industrial civilization,energy crisis and envi-ronmental pollution are serious problems that threaten human beings need to be *** technolo-gies that can use clean solar light energy to manufacture clean H2 fuel and high value chemicals provide a feasibility to solve the above ***,traditional photo-catalytic water splitting mainly focused on the utilization of photogenerated electrons to produce H_(2) which leads to the waste of the *** is vital to develop photocatalysts capable of synchronously utilizing photogenerated elec-trons and holes for the future practical *** this paper,the latest progress of photocatalytic system with collaborative photooxidation-reduction application has been summarized,and water splitting for H_(2) production combining different organic synchronous reaction process has been ***,the prospect of photocatalysts capable of synchronously utilizing photogenerated elec-trons and holes are proposed.

关键词： Photocatalysis Hydrogen generation Synchronous reaction Organic synthesis

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Expression of Bone-related Genes in Bone Marrow MSCs after Cyclic Mechanical Strain: Implications for Distraction Osteogenesis

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International Journal of Oral Science 2009年第3期1卷 143-150页

作者： Meng-chun Qi Shu-juan Zou Li-chi Han hai-xiao Zhou Jing hu State Key Laboratory of Oral Diseases Sichuan University Chengdu China Department of Stomatology North-China Coal Medical College Tangshan China

Aim Understanding the response of mesenchymal stem cells （MSCs） to mechanical strain and their consequent gene expression patterns will broaden our knowledge of the mechanobiology of distraction osteogenesis. Methodology In this study, a single period of cyclic mechanical stretch （0.5 Hz, 2,000 με） was performed on rat bone marrow MSCs. Cellular proliferation and alkaline phosphatase （ALP） activity was examined. The mRNA expression of six bone-related genes （Ets-1, bFGF, IGF-Ⅱ, TGF-β, Cbfal and ALP） was detected using real-time quantitative RT-PCR. Results The results showed that mechanical strain can promote MSCs proliferation, increase ALP activity, and up-regulate the expression of these genes. A significant increase in Ets-1 expression was detected immediately after mechanical stimulation, but Cbfal expression became elevated later. The temporal expression pattem of ALP coincided perfectly with Cbfal. Conclusion The results of this study suggest that mechanical strain may act as a stimulator to induce differentiation of MSCs into osteoblasts, and that these bone-related genes may play different roles in the response of MSCs to mechanical stimulation.

关键词： distraction osteogenesis mechanical strain mesenchymal stem cell (MSC) osteogenic factor gene expression

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基于生物信息学的胃癌关键糖酵解相关差异表达基因的鉴定及预后模型的构建

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医学分子生物学杂志 2025年第1期22卷 76-83页

作者：许娟胡琬琪仙桃市第一人民医院肿瘤科湖北省仙桃市433000 仙桃市第一人民医院胃肠外科湖北省仙桃市433000

目的应用生物信息学工具探索糖酵解相关基因(glycolysis-related genes,GRGs)在胃癌中的表达及基于其所构建的风险评分模型与胃癌患者预后的关系。方法从癌症基因组图谱(the cancer genome atlas,TC-GA)数据库下载胃癌组织转录组和临床... 详细信息

目的应用生物信息学工具探索糖酵解相关基因(glycolysis-related genes,GRGs)在胃癌中的表达及基于其所构建的风险评分模型与胃癌患者预后的关系。方法从癌症基因组图谱(the cancer genome atlas,TC-GA)数据库下载胃癌组织转录组和临床特征数据。从基因集富集分析(gene set enrichment analysis,GSEA)数据库中获得GRGs集。使用limma包来识别胃癌组织中差异表达的GRGs。使用单因素Cox回归分析筛选预后相关的GRGs,应用LASSO回归分析构建基于GRGs的预后预测模型。根据Cox回归分析确定的独立预后危险因素构建Nomogram图。最后,分析22种浸润性免疫细胞与GRGs、风险评分模型相关性。结果鉴定出21个差异表达的GRGs,其主要富集在酒精代谢过程和酪氨酸代谢途径。利用LASSO和Cox模型筛选出5个预后相关的糖酵解基因(ADH1B、ADH4、CLDN9、VCAN及LHX90),构建预测胃癌预后风险评分模型。低风险评分的胃癌患者总生存时间明显优于高风险评分的胃癌患者,ROC曲线分析显示该模型预测胃癌患者1年、3年及5年生存期曲线下面积(area under curve,AUC)值分别为0.602、0.680和0.802,发现该模型预测患者生存期的效能优于肿瘤分期及分级。单因素和多因素Cox分析显示构建的模型、患者的年龄、性别及肿瘤分期、分级是胃癌患者独立预后因素。基于独立危险因素构建了用来预测胃癌患者生存Nomogram图。最后,发现CD8 T细胞和辅助性滤泡T细胞比例在高危组中明显降低,而M0型和M2型巨噬细胞比例在高危组中明显高于低危组。辅助性滤泡T细胞与ADH1B表达水平、VCAN表达水平和风险评分呈负相关。M2型巨噬细胞与VCAN表达水平和风险评分呈正相关。结论研究筛选出胃癌预后相关的5个GRGs构建的模型可作为评估胃癌患者预后的可靠工具,为胃癌提供潜在的糖酵解治疗靶点。

关键词：胃癌糖酵解差异表达基因 Cox回归预后指标诺莫图

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Synthesis, Structure Characterization and Biological Activity of a Novel Amino Acid Salt (HAla)_8(H_30)_(10)[PMo_(12)O_(40)]_6·22H_2O

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Chemical Research in Chinese Universities 2001年第4期17卷 356-361页

作者： HAN Zheng-bo WANG En-bo LUAN Guo-you hu Chang-wen CHANG Ya-ping LI juan hu Ning-hai JIA Heng-qing Faculty of Chemistry Northeast Normal UniversityChangchun 130024P.R.China College of Medicine Jilin UniversityChangchun 130021P.R.China Changchun Institude of Applied Chemistry Chinese Academy of Sciences.Changchun 130022P.R.China

A novel compound was synthesized and characterized by means of elemental analysis, IR and UV spectra, TG, CV and single crystal X-ray diffraction. The compound crystallized in an orthorhombic space group C222 with a = 1. 622 4(3) nm, b=3. 498 4(7) nm, c=l. 301 5(3) nm,V=7. 387(3) nm3, Z = 6, R1 = 0. 037 3, wR2 = 0. 114 0. The Ala (Ala = alanine) molecules were protonated at the amino nitrogen N (1) and the C (2) of Ala group with the terminal oxygen atom O(15), O(14), O(26) and O(27) of the polyoxometalates participating in the hydrogen bond network. The anti-tumor activity of the title compound was estimated against Hela and Pc-3m cancer cells.

关键词： Polyoxometalate Ala Crystal structure Antitumor activity

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Template-directed Synthesis of Carboxylic Acid-functionalized Tetracationic Cyclophane

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Chemical Research in Chinese Universities 2007年第6期23卷 745-748页

作者： XI hai-tao SUN Xiao-qiang CHEN juan MENG Qi PAN Yi hu Hong-wen Department of Chemistry Nanjing University Nanjing 210093 P. R. China Key Laboratory of Fine Chemical Engineering Jiangsu Polytechnic University Changzhou 213164 P. R. China

Introduction Although devices based on pseudorotaxanes, cantenanes, and rotaxanes are commercialized yet, several researches have been done on the applications of these donor-acceptor molecules. A promising area is the field of nanotechnology. The miniaturization of semi-conductor technology by the ＂top-down＂ approach is limited by the wavelength of the radiation used in the lithography to prepare electronic components. Therefore, the ＂bottom-up＂ may be more suitable to create devices with sizes down to the molecular level. A molecular or supramolecular system, in which the relative position of the component parts can be altered by an external stimulus, a molecular switch, can act as the basic unit for the creation of new molecular devices .

关键词： Ethyl 3,5-bis(bromomethyt) benzoate Cyetophane Template Synthesis

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Diflurobenzothiadiazole core-based noncovalently fused small molecule acceptor exhibiting over 12% efficiency and high fill factor

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Journal of Energy Chemistry 2020年第12期29卷 7-13页

作者： Qin Chang Honggang Chen Jun Yuan Yunbin hu Jiefeng hai Wei Liu Fangfang Cai juan Hong Xuxian Xiao Yingping Zou College of Chemistry and Chemical Engineering Central South UniversityChangsha 410083HunanChina Guangxi Key Laboratory of Electrochemical and Magneto-Chemical Functional Materials College of Chemistry and BioengineeringGuilin University of TechnologyGuilin 541004GuangxiChina School of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450001HenanChina

The versatility and flexibility of organic photoelectric materials endow organic photovoltaic cells fine function modulation and huge commercial potential. In this work, a new noncovalent fused-ring small molecule acceptor(SMA) BID-4 F has been synthesized for high-efficient organic solar cells(OSCs). BID-4 F consists of a diflurobenzothiadiazole(DFBT) core, ladder-like indacenodithiophene(IDT) spacers, and dicyanoindanone electron-withdrawing end groups, which are supposed to be conformationally interlocked by noncovalent interactions, leading to good molecular planarity. In addition, compact solid state stacking was revealed by UV–vis–NIR absorption spectrum. The optimized PM6:BID-4 F based device delivers an eminent power conversion efficiency(PCE) of 12.30% with a high open-circuit voltage(Voc) of 0.92 V and a high fill factor(FF) of 74.3%. Most importantly, the PCE and FF are among one of the highest values reported for the OSCs based on the unfused-ring SMAs. Overall, our work demonstrates that the unfused ring central framework with high molecular planarity through noncovalent interactions provides a good strategy to construct highly efficient SMAs.

关键词： Diflurobenzothiadiazole core Noncovalently fused small molecule acceptor High efficiency High fill factor

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