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A novel ASYMMETRIC LEAVES1 and 2 function in Arabidopsis floral organ development

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A novel ASYMMETRIC LEAVES1 and 2 function in Arabidopsis flo...

2006年拟南芥研究学术研讨会

作者： Ben Xu Aiwu Dong hai huang national laboratory of plant molecular genetics, shanghai institute of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 200032, china State Key laboratory of Genetic Engineering, Department of Biochemistry, School of Life sciences, Fudan University, shanghai 200433, china

＜正＞The Arabidopsis ASYMMETRIC LEAVES1 and 2 （AS1 and AS2） genes are demonstrated to repress class I KNOTTED1-like homeobox genes and to promote the leaf adaxial specifying genes PHABULOSA, PHAVOLUTA and REVOLUTA during leaf morphogenesis. However, very little is known about AS1/2 roles in flower development, although as1 and as2 flowers appear not completely normal. To investigate the AS1/2 function in flower development, we constructed double mutants by combining as1 or as2 with

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Transcriptional, post-transcriptional and post-translational regulations of gene expression during leaf polarity formation

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Cell Research 2007年第6期17卷 512-519页

作者： Lin Xu Li Yanghai huang national laboratory of plant molecular genetics, shanghai institute of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, 300 Fenglin Road, shanghai 200032, china national laboratory of plant molecular genetics Shanghai Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Present address: IBMP CNRS Universite Louis Pasteur 67084 Strasbourg France Present address: Department of Biology University of Pennsylvania Philadelphia PA 19104 USA

Leaf morphogenesis requires the establishment of adaxial-abaxial polarity after primordium initiation from the shootapical meristem (SAM). Several families of transcription factors are known to play critical roles in promoting adaxial orabaxial leaf fate. Recently, post-transcriptional gene silencing pathways have been shown to regulate the establishmentof leaf polarity, providing novel and exciting insights into leaf development. For example, microRNAs (miR165/166)and a trans-acting siRNA (TAS3-derived tasiR-ARF) have been shown to repress the expression of several key transcrip-tion factor genes. In addition, yet another level of regulation, post-translational regulation, has been revealed recently bystudies on the role of the 26S proteasome in leaf polarity. Although our understanding regarding the molecular mecha-nisms underlying establishment of adaxial-abaxial polarity has greatly improved, there is still much that remains *** review aims to discuss recent progress, as well as the remaining questions, regarding the molecular mechanismsunderlying leaf polarity formation.

关键词： Arabidopsis leaf development polarity formation

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

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Cell Research 2001年第2期11卷 149-155页

作者： huang JIAN QIU, ZHI MING WEI, hai LONG AN, YU XIAN ZHU national laboratory of plant molecular genetics, institute of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 200032, china national La national laboratory of plant molecular genetics Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences Chinese Academy of Sciences Shanghai China national laboratory of plant Genetic Engineering and Protein Engineering College of Life Science Peking University Beijing China

Immature embryos of rice varieties 'Xiushui11' and 'Chunjiang 11' precultured for 4d were infected and transformed by Agrobacterium tumefaciens strain EHA101/pExT7 (containing the spider insecticidal gene). The resistant cant were transferred onto the differentiation medium and plants were regenerated. The transformation frequency reached 56%-72% measured as numbers of Geneticin (G418)-resistant calli produced and 36%-60% measured as numbers of transgenic plants regenerated, respectively. PCR and Southern blot analysis of transgenic plants confirmed that the T-DNA had been integrated into the rice genome. Insect bioassays using T1 transgenic plants indicated that the mortality of the leaffolder (Cnaphalocrasis medinalis) after 7d of leaf feeding reached 38%-61% and the corrected mortality of the striped stem borer (Chilo suppressalis) after 7d of leaf feeding reached 16%-75%. The insect bioassay results demonstrated that the transgenic plants expressing the spider insecticidal protein conferred enhanced resistance to these pests.

关键词： Rice Agrobacterium tumefaciens spider insecticidal gene transgenic plant Leaffolder striped stem borer insect bioassay.

Role of oxyR from Sinorhizobium meliloti in Regulating the Expression of Catalases

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Acta Biochimica et Biophysica Sinica 2005年第6期37卷 421-428页

作者： Li EUO~(1.3) Ming-Sheng QI~(1.3) Shi-Yi YAO~2 hai-Ping CHENG~2 Jia-Bi ZHU~1 Guan-Qiao YU~1 1 national laboratory of plant molecular genetics, institute of plant physiology and ecology, shanghai institutes for biological sciences.chinese academy of sciences, shanghai 200032. china 2 biological sciences Department, Lehman College, the City University of New York, Bronx, NY 10468. USA, 3 Graduate School of the chinese academy of sciences, shanghai 200032. china national laboratory of plant molecular genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200032 China Graduate School of the chinese academy of sciencesshanghai 200032 China biological sciences Department Lehman College the City University of New YorkBronx NY 10468 USA

The process of symbiotic nitrogen fixation results in the generation of reactive oxygen speciessuch as the superoxide anion（O2-）and hydrogen peroxide（H2O2）.The response of rhizobia to these toxicoxygen species is an important factor in nodulation and nitrogen *** Sinorhizobium meliloti,oneoxyR homologue and three catalase genes,katA,katB,and katC were detected by sequence *** gene is located next to and divergently from katA on the *** investigate the possible rolesof oxyR in regulating the expression of catalases at the transcriptional level in ***,an insertion mutantof this gene was *** mutant was more sensitive and less adaptive to H2O2than the wild typestrain,and total catalase/peroxidase activity was reduced approximately fourfold with the OxyR mutationrelative to *** activities of KatA and KatB and the expression of katA::lacZ and katB::lacZ promoterfusions were increased in the mutant strain compared with the parental strain grown in the absence of H2O2,indicating that katA and katB are repressed by ***,when exposed to H2O2,katA expressionwas also increased in both *** and Escherichia *** exposed to H2O2,OxyR is convertedfrom a reduced to an oxidized form in *** concluded that the reduced form of OxyR functions as arepressor of katA and katB ***,in the presence of H2O2,reduced OxyR is converted to theoxidized form of OxyR that then results in increased katA *** further showed that oxyR expressionis autoregulated via negative feedback.

关键词： Sinorhizobium meliloti OxyR catalase katA H2O2

Alpha-picolinic acid,a fungal toxin and mammal apoptosis-inducing agent,elicits hypersensitive-like response and enhances disease resistance in rice

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Cell Research 2004年第1期14卷 27-33页

作者： hai Kuo ZHANG~1, Xin ZHANG~1, Bi Zeng MAO~(2,) Qun LI~1, Zu Hua HE~(1,2*) ~1SHARF and national laboratory of plant molecular genetics, shanghai institute of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 200032, china. ~2Biotechnology institute, Zhejiang University, Hangzhou 310029, china. SHARF and national laboratory of plant molecular genetics Shanghai Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Biotechnology institute Zhejiang University Hangzhou China

Alpha-picolinic acid (PA), a metabolite of tryptophan and an inducer of apoptosis in the animal cell, has been reported to be a toxin produced by some of plant fungal pathogens and used in screening for disease resistant mutants. Here, we report that PA is an efficient apoptosis agent triggering cell death of hypersensitive-like response in planta. Confirmed by Fluorescence Activated Cell Sorter (FACS), rice suspension cells and leaves exhibited programmed cell death induced by PA. The PA-induced cell death was associated with the accumulation of reactive oxygen species that could be blocked by diphenylene iodonium chloride, indicating that the generation of reactive oxygen species was NADPH-oxidase dependent. We also demonstrated the induction of rice defense-related genes and subsequent resistant enhancement by PA against the rice blast fungus Magnaporthe grisea. Hence, it was concluded that the PA-stimulated defense response likely involves the onset of the hypersensitive response in rice, which also

关键词： picolinic acid cell death hypersensitive responses Magraporche grisea rice.

plant fertility defects induced by the enhanced expression of microRNA167

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Cell Research 2006年第5期16卷 457-465页

作者： Peng Ru~1 Lin Xu~1 Hong Ma~(2,3) hai huang~(1,2) ~1national laboratory of plant molecular genetics,shanghai institute of plant physiology and ecology,shanghai institute for biological sciences,300 Fenglin Road,shanghai 200032,china ~2SIBS-SJTU-PSU Joint Center for Life sciences,shanghai institute of plant physiology and Ecologgy,shanghai institute for biological sciences,300 Fenglin Road,shanghai 200032,china ~3Department of Biology and the Huck institute for the Life sciences,The Pennsylvania State University,University Park,PA 16802,USA national laboratory of plant molecular genetics Shanghai Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences Shanghai China SIBS-SJTU-PSU Joint Center for Life sciences Shanghai Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences Shanghai China Department of Biology and the Huck institute for the Life sciences The Pennsylvania State University University Park USA

The plant hormone auxin plays a critical role in regulating plant growth and *** advances havebeen made in the understanding of auxin response pathways,primarily by the characterization of auxin response mu-tants in *** addition,microRNAs (miRNAs) have been shown to be critical regulators of genes importantfor normal plant development and ***,little is known about possible interactions between miRNAsand hormonal signaling during norlnal *** we show that an Arabidopsis microRNA,miR167,whichhas a complementary sequence to a portion of the A UXIN RESPONSE FACTOR6 (ARF6) and ARF8 mRNAs,can causetranscript degradation for ARFS,but not for *** report phenotypic characterizations of35S::MIR167b transgeniclines,and show that severe 35S::MIR167b transgenic lines had phenotypes similar to those of an arf6 arf8 double *** transgenic phenotypes suggest that miR167 may repress ARF6 at the level of *** demonstrate that thetransgenic plants are defective in all four whorls of floral *** the transgenic flowers,filaments were abnormallyshort,anthers could not properly release pollen,and pollen grains did not *** results provide an importantlink between the miRNA-mediated regulatory pathway of gene expression and the auxin signaling network promotingplant reproductive development.

关键词： Arabidopsis auxin response factor microRNA167 floral organ pollen germination

Salt-responsive genes in rice revealed by cDNA microarray analysis

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Cell Research 2005年第10期 50-64页

作者： Dai Yin CHAO1,3, Yong hai LUO1,3, Min SHI1, Da LUO1,2, Hong Xuan LIN1,2,* 1national Key laboratory of plant molecular genetics, shanghai institute of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, 300 Fenglin Road, shanghai 200032, china, 2SHARF laboratory, shanghai institute of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, 300 Fenglin Road, shanghai 200032, china, 3Graduate School of the chinese academy of sciences (D.C.), 19 Yuquan Road, Beijing 100039, china

We used cDNA microarrays containing ~9,000 unigenes to identify 486 salt responsive expressed sequence tags (ESTs) (representing ~450 unigenes) in shoots of the highly salt-tolerant rice variety, Nona Bokra (Oryza sativa L. ssp. Indica pv. Nona). Some of the genes identified in this study had previously been associated with salt stress. However the majority were novel, indicating that there is a great number of genes that are induced by salt exposure. Analysis of the salt stress expression profile data of Nona provided clues regarding some putative cellular and molecular processes that are undertaken by this tolerant rice variety in response to salt stress. Namely, we found that multiple transcription factors were induced during the initial salt response of shoots. Many genes whose encoded proteins are implicated in detoxification, protectant and transport were rapidly induced. Genes supporting photosynthesis were repressed and those supporting carbohydrate metabolism were altered. Commonality among the genes induced by salt exposure with those induced during senescence and biotic stress responses suggests that there are shared signaling pathways among these processes. We further compared the transcriptome changes of the salt-sensitive cultivar, IR28, with that of Nona rice. Many genes that are salt responsive in Nona were found to be differentially regulated in IR28. This study identified a large number of candidate functional genes that appear to be involved in salt tolerance and further examination of these genes may enable the molecular basis of salt tolerance to be elucidated.

关键词： cDNA microarray rice salt stress signaling crosstalk transcriptome.

molecular mechanism of G-protein-regulated chloroplast development

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Molecular mechanism of G-protein-regulated chloroplast devel...

植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Jirong huang national Key laboratory of plant molecular genetics,institute of plant physiology and ecology, shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200032,china

Heterotrimeric G-proteins have been demonstrated to play an important role in many physiological processes in *** first evidence for the involvement of G-proteins in chloroplast development came from pharmaceutical studies on tomato hypocotyl cells of the aurea mutant that is defective in phytochrome A(Neuhaus et al.,1993).It

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DTU1, a novel locus in Arabidopsis suppresses class I KNOX genes during plant development

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DTU1, a novel locus in Arabidopsis suppresses class I KNOX g...

2006年拟南芥研究学术研讨会

作者： Limin Pi hai huang national laboratory of plant molecular genetics, shanghai institute of plant physiology and ecology, shanghai institutes for biological sciences, shanghai 200032, china

＜正＞Silence of the class I knottedl-like homeobox （KNOX） genes at PO leaf stage is crucial for proper leaf primordium initiation. Two important genes, ASYMMETRIC LEAVES1（AS1） and ASYMMETRIC LEAVES2 （AS2） in Arabidopsis play roles in suppressing class I KNOX genes for leaf initiation and maintaining their silencing state in subsequent leaf development. Loss of function in AS1 and AS2 result in lobed leaves and outgrowths appearing on leaf surfaces, similar to those with ectopic expression of class I KNOX genes

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