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Nodulin 26-like intrinsic protein Cs NIP2;2 is a silicon influx transporter in Cucumis sativus L.

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Journal of Integrative Agriculture 2022年第3期21卷 685-696页

作者： DUAN Yao-ke su Yan HAN Rong suN Hao GONG hai-jun Shaanxi Engineering Research Center for Vegetables/College of Horticulture Northwest A&F UniversityYangling 712100P.R.China Henan Key Laboratory of Ion-Beam Bioengineering School of Agricultural SciencesZhengzhou UniversityZhengzhou 450000P.R.China

Nodulin 26-like intrinsic proteins(NIPs) are a family of channel-forming transmembrane proteins that function in the transport of water and other small molecules.Some NIPs can mediate silicon transport across plasma membranes and lead to silicon accumulation in plants,which is beneficial for the growth and development of plants.Cucumber is one of the most widely consumed vegetables;however,the functions of NIPs in this crop are still largely unknown.Here,we report the functional characteristics of Cs NIP2;2.It was found that Cs NIP2;2 is a tandem repeat of Cs NIP2;1,which had been demonstrated to be a silicon influx transporter gene.Cs NIP2;2 has a selectivity filter composed of cysteine,serine,glycine and arginine(CSGR),which is different from all previously characterized silicon influx transporters in higher plants at the second helix position.Xenopus laevis oocytes injected with Cs NIP2;2 c RNA demonstrated a higher uptake of silicon than the control,and the uptake remained unchanged under low temperature.Cs NIP2;2 was found to be expressed in the root,stem,lamina and petiole,and exogenous silicon treatment decreased its expression in the stem but not in other tissues.Transient expression of Cs NIP2;2-e GFP fusion sequence in onion epidermal cells showed that Cs NIP2;2 was localized to the cell nucleus,plasma membrane and an unknown structure inside the cell.The results suggest that Cs NIP2;2 is a silicon influx transporter in cucumber,and its subcellular localization and the selectivity filter are different from those of the previously characterized silicon influx transporters in other plants.These findings may be helpful for understanding the functions of NIPs in cucumber plants.

关键词： cucumber(Cucumis sativus L.) nodulin 26-like intrinsic membrane protein(NIP) silicon influx transporter aromatic/arginine selectivity filter

The mechanism by which hyperbaric oxygen treatment alleviates spinal cord injury: genome-wide transcriptome analysis

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Neural Regeneration Research 2022年第12期17卷 2737-2742页

作者： Zhen-Cheng sun Fang Liang Jing Yang Yong hai Qing-jun su Xue-Hua Liu Department of Orthopedic surgery Beijing Chaoyang HospitalCapital Medical UniversityBeijingChina Department of Hyperbaric Oxygen Beijing Chaoyang HospitalCapital Medical UniversityBeijingChina

Accumulating studies have demonstrated that hyperbaric oxygen(HBO)treatment alleviates spinal cord injury(SCI).However,the underlying mechanism by which HBO alleviates SCI remains to be elucidated.In this study,we performed genome-wide transcriptional profiling of the spinal cord between SCI mice and mice that received HBO treatment by high-throughput RNA sequencing at 1 week after SCI.We also compared genome-wide transcriptional profiles from SCI mice and sham-operated mice.We found 76 differentially co-expressed genes in sham-operated mice,SCI mice,and HBO-treated SCI mice.Using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis,we identified the biological characteristics of these differentially expressed genes from the perspectives of cell component,biological process,and molecular function.We also found enriched functional pathways including ferroptosis,calcium signaling pathway,serotonergic synapse,hypoxia-inducible factor-1 signaling pathway,cholinergic synapse,and neuroactive ligand-receptor interaction.We performed quantitative reverse transcription-polymerase chain reaction and validated that HBO treatment decreased the expression of Hspb1(heat shock protein beta 1),Hmox1(heme oxygenase 1),Ftl1(ferritin light polypeptide 1),Tnc(tenascin C)and Igfbp3(insulin-like growth factor binding protein 3)and increased the expression of Slc5a7(solute carrier family 5 choline transporter member 7)after SCI.These results revealed the genome-wide transcriptional profile of the injured spinal cord after HBO treatment.Our findings contribute to a better understanding of the mechanism by which HBO treats SCI and may provide new targets for SCI intervention.

关键词： Ftl1 genome-wide transcriptome Hmox1 Hspb1 hyperbaric oxygen Igfbp3 Slc5a7 spinal cord injury Tnc

Design of Self-Recon gurable Multiarm Robot Mechanism Based on Deployable Kinematic Chains

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Chinese Journal of Mechanical Engineering 2020年第5期 44-60页

作者： Fu-Qun Zhao Sheng Guo hai-jun su hai-Bo Qu Ya-Qiong Chen School of Mechanical Electronic and Control Engineering Beijing Jiaotong University Department of Mechanical and Aerospace Engineering The Ohio State University

As the structures of multiarm robots are serially arranged, the packaging and transportation of these robots are often inconvenient. The ability of these robots to operate objects must also be improved. Addressing this issue, this paper presents a type of multiarm robot that can be adequately folded into a designed area. The robot can achieve different operation modes by combining different arms and objects. First, deployable kinematic chains(DKCs) are designed, which can be folded into a designated area and be used as an arm structure in the multiarm robot mechanism. The strategy of a platform for storing DKCs is proposed. Based on the restrictions in the storage area and the character-istics of parallel mechanisms, a class of DKCs, called base assembly library, is obtained. subsequently, an assembly method for the synthesis of the multiarm robot mechanism is proposed, which can be formed by the connection of a multiarm robot mechanism with an operation object based on a parallel mechanism structure. The formed parallel mechanism can achieve a reconfigurable characteristic when different DKCs connect to the operation object. Using this method, two types of multiarm robot mechanisms with four DKCs that can switch operation modes to perform different tasks through autonomous combination and release operation is proposed. The obtained mechanisms have observable advantages when compared with the traditional mechanisms, including optimizing the occupied volume during transportation and using parallel mechanism theory to analyze the switching of operation modes.

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πN Elastic Scattering and Resonances in Quark Potential Model

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Communications in Theoretical Physics 2008年第4期49卷 987-992页

作者： WANG hai-jun su jun-Chen Center for Theoretical Physics Jilin University Changchun 130023 China

夸克潜力模型被用来调查 πN 系统散布的低精力的橡皮。模型潜力由 t 隧道和 s 隧道 one-gluon 交换潜力和限制潜力的泛音振荡器组成。借助于共振的组方法，为 πN 系统的一个非局部的有效潜力被导出从内部夸克潜力并且过去常计算散布... 详细信息

夸克潜力模型被用来调查 πN 系统散布的低精力的橡皮。模型潜力由 t 隧道和 s 隧道 one-gluon 交换潜力和限制潜力的泛音振荡器组成。借助于共振的组方法，为 πN 系统的一个非局部的有效潜力被导出从内部夸克潜力并且过去常计算散布阶段移动的 πN 橡皮。由考虑 QCD 重正常化的效果，纺纱轨道的联合和簇(q) 的颜色八重唱的贡献的抑制并且(qqq ) ，数字结果在对试验性的数据的相当好的同意。一样的模特儿和方法被雇用调查可能的 πN 回声。为这个目的，共振的组方程被转变成非局部的有效 πN 相互作用潜力在被包括的一个标准 Schrödinger 方程。由变化方法解决 Schrödinger 方程，我们能复制一些当前担心的 πN 回声的群众。

关键词：夸克势模式介子-中子交互作用介子弹性散射

Transcriptional search to identify and assess reference genes for expression analysis in Solanum lycopersicum under stress and hormone treatment conditions

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Journal of Integrative Agriculture 2022年第11期21卷 3216-3229页

作者： DUAN Yao-ke HAN Rong su Yan WANG Ai-ying LI Shuang suN Hao GONG hai-jun Shaanxi Engineering Research Center for Vegetables/College of Horticulture Northwest A&F UniversityYangling 712100ShaanxiP.R.China Henan Key Laboratory of Ion-Beam Bioengineering College of Agricultural SciencesZhengzhou UniversityZhengzhou 450000HenanP.R.China

Tomato(Solanum lycopersicum)is a model plant for research on fruit development and stress response,in which gene expression analysis is frequently conducted.Quantitative PCR(qPCR)is a widely used technique for gene expression analysis,and the selection of reference genes may affect the accuracy of results and even conclusions.Although there have been some frequently used reference genes in tomato,it has been shown that the expressions of some of these genes are not constant in different tissues and environmental conditions.Moreover,little information on genomic identification of reference genes is available in tomato.Here,we mined the publicly available transcriptional sequencing data and screened out fifteen candidate reference genes,and the expression stability of these candidate genes and seven traditionally used ones were evaluated under stress and hormone treatment.The results showed that over half of the selected candidate references were housekeeping genes in tomato cells.Among the candidate reference genes and the traditionally used ones,the most stably expressed genes varied under different treatments,and most of these genes were recommended as preferred reference genes at least once except Solyc04g009030 and Solyc07g066610,two traditionally used reference genes.This study provides some novel reference genes in tomato,and the preferred reference genes under different environmental stimuli,which may be useful for future research.Our study suggests that excavating stably expressed genes from transcriptome sequencing data is a reliable approach to screening reference genes for qPCR analysis.

关键词： tomato(Solanum lycopersicum) gene expression qPCR reference gene expression stability

Stenting of the Portal Vein Combined with Different Numbers of Iodine-125 Seed Strands： Dosimetric Analyses

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Chinese Medical Journal 2017年第18期130卷 2183-2189页

作者： Li-Hong Yao Liang su Lu Liu hai-Tao sun jun-Jie Wang Department of Radiation Oncology Peking University Third Hospital Beijing 100191 China Department of Radiation Oncology The People's Hospital of Zhangqiu Jinan Shandong 250200 China

Background: Portal-vein stent combined with one iodine-125 ( 125 I) seed strand has become a new treatment for portal vein tumor thrombosis. However, dosimetric aspects of this irradiation stent have not been reported. Therefore, we aimed to undertake dosimetric analyses comparing portal-vein stents combined with different numbers of 125 I seed strands. Methods: A water cylinder was created by a treatment-planning system to simulate a portal-vein stent. The stent was combined with one, two, or three 125 I seed strands (Groups I, II, and III, respectively). At different prescribed doses (PDs), 125 I seeds of identical activities were loaded on Groups I–III. Conformation number (CN), external volume index, and homogeneity index were calculated. Linear regression analyses were used to evaluate the obtained data. Results: For identical 125 I seed activity, when the 125 I seed strand increased from one chain to two, D 90 (dose delivered to 90% of the target volume) increased by ≥184%; when it increased from two chains to three, D 90 increased by ≥63%. When the PD was 105 Gy and 125 I seed strands increased from one chain to two, V 100 (percentage of the target volume receiving ≥90% of the PD) increased by 158–249%; when it increased from two chains to three, V 100 increased by 7–175%. CN was correlated positively with 125 I seed activity ( B = 0.479, P < 0.001) and number of 125 I seed strands ( B = 0.201, P < 0.001) and was independent of PD ( B = −0.002, P = 0.078). Conclusions: A portal-vein stent combined with a single 125 I seed strand could not meet dosimetry requirements. For a stent combined with two 125 I seed strands, when the PD was 105 Gy and seed activity was 0.7 mCi, the dose distribution could satisfy dosimetry requirements. For a stent combined with three 125 I seed strands, if the PD was 105, 125, or 145 Gy, the recommended seed activities were 0.5, 0.5, and 0.6 mCi, respectively.

关键词：粒子束门静脉支架同数放射性治疗计划系统种子活动 V100

Effects of grain refinement on cast structure and tensile properties of superalloy K4169 at high pouring temperature

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China Foundry 2016年第2期13卷 101-106页

作者： Zi-qi Jie jun Zhang Tai-wen Huang Lin Liu hai-jun su Yan-li Shi Heng-zhi Fu State Key Laboratory of Solidification ProcessingNorthwestern Polytechnical UniversityXi'an 710072China Xi'an Jiaotong University City College Xi'an 710072China

In order to improve the filling ability of large complex thin wall castings, the pouring temperature should be increased, but this will result in the grain coarsening. To overcome this problem, two kinds of grain refiners of Co-Fe-Nb and Cr-Fe-Nb ternary alloys, which contain high stability compound particles, were prepared. The effects of the refiners on the as-cast structures and tensile properties of the K4169 superalloy with different casting conditions were studied by analyzing specimens 110 mm long and 20 mm in diameter.Results showed that the mixture addition of the two refiners in the melt of K4169 can reduce the columnar grain region and decrease the equiaxed grain size greatly. After refinement, the amount of Laves phase decreases and its morphology changes from island to blocky structure. The carbides in the fine grain samples are fine and dispersive. Meanwhile, the porosity in specimens is decreased due to grain refinement. As a result, the yield strength, ultimate strength and the elongation of the specimens are increased. The grain refinement mechanisms are also discussed.

关键词： superalloy K4169 grain refinement tensile properties

Prognostic factors of spontaneously ruptured hepatocellular carcinoma

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World Journal of Gastroenterology 2015年第24期21卷 7488-7494页

作者： Xiang-jun Han Hong-Ying su hai-Bo Shao Ke Xu Department of Radiology the First Hospital of China Medical University

AIM: To evaluate the prognostic factors in patients with spontaneously ruptured hepatocellular carcinoma(HCC). METHODS: Seventy-nine patients experiencing spontaneous rupture of HCC between April 2004 and August 2014 were enrolled in this study. The clinical features, treatment modalities and outcomes were reviewed. The statistical methods used in this work included univariate analysis, Kaplan-Meier survival analysis with log-rank tests, and multivariate analysis using a Cox regression hazard model. REsuLTS: Of the 79 patients with HCC rupture, 17(21.5%) underwent surgery, 32(40.5%) underwent transarterial embolization(TAE), and 30(38%) received conservative treatment. The median survival time was 125 d, and the mortality rate at 30 d was 27.8%. Multivariate analysis revealed that lesion length(HR = 1.46, P survival after HCC rupture. The BarcelonaClinic Liver Cancer(BCLC) stage was also an important prognostic factor; the median survival times for BCLC stages A, B and C were 251, 175 and 40 d, respectively(P survival. Other factors play only a small role in overall survival.

关键词： Hepatocellular carcinoma Rupture Prognosis surgery Transarterial embolization

Changes in proteins related to early nerve repair in a rat model of sciatic nerve injury

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Neural Regeneration Research 2021年第8期16卷 1622-1627页

作者： Yu-Song Yuan Fei Yu Ya-jun Zhang su-Ping Niu hai-Lin Xu Yu-Hui Kou Department of Trauma and Orthopedics Peking University People’s HospitalBeijingChina Key Laboratory of Trauma and Neural Regeneration(Peking University) Ministry of EducationBeijingChina National&Local Joint Engineering Research Center of Orthopedic Biomaterials Department of Bone&Joint SurgeryPeking University Shenzhen HospitalShenzhenGuangdong ProvinceChina National Center for Trauma Medicine BeijingChina Office of Academic Research Peking University People’s HospitalBeijingChina Diabetic Foot Treatment Center Peking University People’s HospitalBeijingChina

Peripheral nerves have a limited capacity for self-repair and those that are severely damaged or have significant defects are challenging to repair. Investigating the pathophysiology of peripheral nerve repair is important for the clinical treatment of peripheral nerve repair and regeneration. In this study, rat models of right sciatic nerve injury were established by a clamping method. Protein chip assay was performed to quantify the levels of neurotrophic, inflammation-related, chemotaxis-related and cell generation-related factors in the sciatic nerve within 7 days after injury. The results revealed that the expression levels of neurotrophic factors(ciliary neurotrophic factor) and inflammationrelated factors(intercellular cell adhesion molecule-1, interferon γ, interleukin-1α, interleukin-2, interleukin-4, interleukin-6, monocyte chemoattractant protein-1, prolactin R, receptor of advanced glycation end products and tumor necrosis factor-α), chemotaxis-related factors(cytokine-induced neutrophil chemoattractant-1, L-selectin and platelet-derived growth factor-AA) and cell generation-related factors(granulocyte-macrophage colony-stimulating factor) followed different trajectories. These findings will help clarify the pathophysiology of sciatic nerve injury repair and develop clinical treatments of peripheral nerve injury. This study was approved by the Ethics Committee of Peking University People's Hospital of China(approval No. 2015-50) on December 9, 2015.

关键词： animal model cell generation chemotaxis clamp injury inflammation injury neurotrophic factor peripheral nerve protein array repair sciatic nerve Wallerian degeneration