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中国植物病理学会2024年学术年会

作者： Mohammad Mazharul Karim hafiz muhammad usman TAN Qin HU Jiajie FAN Fei Rafakat Hussain LUO Chaoxi National Key Laboratory for Germplasm Innovation Utilization of Horticultural Crops Huazhong Agricultural University Department of Plant Pathology College of AgricultureGuizhou University Hubei Key Lab of Plant Pathology College of Plant Science and TechnologyHuazhong Agricultural University

Peach is one of the popular and economically important fruit crops in *** cultivation is hampered due to attacks of anthracnose disease,causing significant economic *** fructicola and Colletotrichum siamense belong to the Colletotrichum gloeosporioides species complex and are considered major pathogens of peach *** of different groups of fungicides is a routine approach for controlling this ***,fungicide resistance is a significant drawback in managing peach anthracnose *** this study,39 isolates of *** and 41 isolates of *** were collected from different locations in various provinces in *** sensitivity of *** and *** to some commonly used fungicides,i.e.,carbendazim,iprodione,fluopyram,and propiconazole,was *** the isolates of *** collected from Guangdong province showed high resistance to carbendazim,whereas isolates collected from Guizhou province were *** ***,isolates collected from Hebei province showed moderate resistance,while those from Shandong province were sensitive to *** the other hand,all the isolates of *** and *** showed high resistance to the dicarboximide(DCF) fungicide iprodione and succinate dehydrogenase inhibitor(SDHI) fungicide fluopyram,indicating natural resistance in these species to these ***,they are all sensitive to the demethylation inhibitor(DMI) fungicide *** cross-resistance was observed between carbendazim and benomyl as they are members of the same methyl benzimidazole carbamate(MBC) *** no correlation of sensitivity was observed between different groups of *** significant differences were found in each fitness parameter between carbendazim-resistant and sensitive isolates in both *** characterization of the β-tubulin 2(TUB2) gene revealed that in ***,the E198 A point mutation was the determin

关键词： Fungicide Resistance in Colletotrichum fructicola and Colletotrichum siamense Causing Peach Anthracnose in China

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Risk of infection with arboviruses in a healthy population in Pakistan based on seroprevalence

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Virologica Sinica 2024年第3期39卷 369-377页

作者： Shengyao Chen muhammad Saqib hafiz Sajid Khan Yuan Bai usman Ali Ashfaq muhammad Khalid Mansoor Abulimti Moming Jing Liu Min Zhou Saifullah Khan Niazi Qiaoli Wu Awais-Ur-Rahman Sial Shuang Tang muhammad Hassan Sarfraz Aneela Javed Sumreen Hayat Mohsin Khurshid Iahtasham Khan muhammad Ammar Athar Zeeshan Taj Bo Zhang Fei Deng Ali Zohaib Shu Shen Key Laboratory of Virology and National Virus Resource Center Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China Faculty of Veterinary Science University of Agriculture FaisalabadFaisalabad 38040Pakistan The Children's Hospital Faisalabad 38000Pakistan Department of Bioinformatics and Biotechnology Government College University FaisalabadFaisalabad 38000Pakistan f Department of Microbiology Faculty of Veterinary and Animal SciencesThe Islamia University of BahawalpurBahawalpur 63100Pakistan Hubei Jiangxia Laboratory Wuhan 430200China Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China Armed Forces Institute of Pathology(AFIP) Rawalpindi 46000Pakistan Department of Clinical Studies Faculty of Veterinary&Animal SciencesPir Mehr Ali Shah Arid Agriculture UniversityRawalpindi 46300Pakistan Institute of Microbiology Government College University FaisalabadFaisalabad 38000Pakistan l Atta-ur-Rahman School of Applied Biosciences National University of Science and TechnologyIslamabad 44000Pakistan Department of Clinical Sciences University of Veterinary and Animal SciencesLahore Sub-campusJhang 35200Pakistan Department of Molecular Pathology National Medical Center DHA Phase 1Karachi 75500Pakistan

Infectious diseases caused by arboviruses are a public health concern in ***,studies on data prevalence and threats posed by arboviruses are *** study investigated the seroprevalence of arboviruses in a healthy population in Pakistan,including severe fever with thrombocytopenia syndrome virus(SFTSV),Crimean-Congo hemorrhagic fever virus(CCHFV),Tamdy virus(TAMV),and Karshi virus(KSIV)based on a newly established luciferase immunoprecipitation system(LIPS)assays,and Zika virus(ZIKV)by enzyme-linked immunosorbent assays(ELISA).Neutralizing activities against these arboviruses were further examined from the antibody positive *** results showed that the seroprevalence of SFTSV,CCHFV,TAMV,KSIV,and ZIKV was 17.37%,7.58%,4.41%,1.10%,and 6.48%,respectively,and neutralizing to SFTSV(1.79%),CCHFV(2.62%),and ZIKV(0.69%)were identified,as well as to the SFTSV-related Guertu virus(GTV,0.83%).Risk factors associated with the incidence of exposure and levels of antibody response were ***,co-exposure to different arboviruses was demonstrated,as thirty-seven individuals were having antibodies against multiple viruses and thirteen showed neutralizing ***,individuals aged40 years,and outdoor workers had a high risk of exposure to *** these results reveal the substantial risks of infection with arboviruses in Pakistan,and indicate the threat from co-exposure to multiple *** findings raise the need for further epidemiologic investigation in expanded regions and populations and the necessity to improve health surveillance in Pakistan.

关键词： Arbovirus Pakistan Seroprevalence Tick-borne virus Mosquito-borne virus

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Impact of Climate Change on Epidemiology of Various Pests of Wheat Crop in Punjab Pakistan

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American Journal of Plant Sciences 2019年第1期10卷 236-247页

作者： Israr Arshad Amer Rasul Syed Ismat Hussain hafiz muhammad usman Aslam Khizar Hayat Malik Najam Ul Hassan Sidra Muqeet Amina Yasir Umar Shabab Nasir Anum Tehseen Directorate of Pest Warning & Quality Control of Pesticides Punjab Pakistan Department of Plant Pathology University of Agriculture Faisalabad Pakistan Department of Plant Pathology and Microbiology Iowa State University USA Horticultural Research Institute Ayub Agricultural Research Institute (AARI) Faisalabad Pakistan Department of Zoology Government College University Faisalabad Faisalabad Pakistan Institute of Agriculture & Resource Economics University of Agriculture Faisalabad Pakistan

Wheat [Triticum aestivum L. (Poaceae)] is the staple diet of people in Pakistan. It is attacked by many types of pests. Therefore the purpose of this study was to assess the impact of climate change on the ecology and epidemiology of various wheat pests in Punjab, Pakistan. Results indicate that maximum weeds hot spots 242 (5.98%) Phalaris minor, 45 (1.18%) wild oat and 203 (5.01%) broad leaf weeds were noted in 2015. Aphid 31 (0.86%) hot spots were recorded in 2016 while maximum army worm 13 (0.26%) hot spots were noted in 2017. Maximum 70 (1.73%) spots of yellow rust and 85 (2.10%) hot spots of brown rust were observed during 2015 while 84 (4.16%) spots of loose smut were observed during 2017. ANOVA shows that years have no significant difference (P > 0.05) but weeks have significant effect on occurrence of these pest incidences except brown rust. But regression ANOVA was significant (P ≤ 0.05) and regression models equations have been developed on the bases of recorded data. Pest incidence was taken as dependent variable Y and weather factors i.e. minimum temperature as X1, maximum temperature as X2, relative humidity as X3 and rain fall as X4 were taken as independent variables. This study will help in recommendations for moving forward aiming at integration of biology of rust and smut diseases of wheat with changing climate for development of resistant varieties for resilient and durable management of these pathogens.

关键词： Climate Change Wheat Weeds Insect Pest Disease Ecology

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Specific Capacitance and Cyclic Stability of Graphene Based Metal/Metal Oxide Nanocomposites:A Review

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Journal of Materials Science & Technology 2015年第7期31卷 699-707页

作者： Abdul Waheed Anwar Abdul Majeed Nadeem Iqbal Wasi Ullah Ahmad Shuaib usman Ilyas Fozia Bibi hafiz muhammad Rafique Nanotechnologies Research Centre Department of PhysicsUniversity of Engineering and Technology Under Graduate Block/School of Physical Sciences University of the Punjab Department of Physics University of the Punjab

Graphene has become a worldwide admired material among researchers and scientists equally due to its unique richness in mechanical strength, electrical conductivity, optical and thermal properties. Re- searchers have explored that the composite materials based on graphene and metal/metal oxide nanostructures possess excellent potential for energy storage technologies. In particular, supercapacitors based on such composite materials have engrossed the extreme interest of researchers for its rapid charging/discharging time, safe operation and longer cyclic constancy. Till now, several fabrication techniques for composite materials and their energy storage applications have been explored. Here, specially, we have concentrated on the hottest research progress for the fabrication of graphene oxide and metal/metal oxide nanocomposites. We also emphasized on the characteristics and properties of supercapacitors fabricated using these composite materials. Moreover, our study is focused on the specific capacitance and cyclic stability of various composites to haul out the most efficient material for supercapacitor applications.

关键词： GrapheneMetal/metal oxide nanostructureSupercapacitor

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Study on the Restriction of Copyright of Visually Impaired Persons Using Works

Study on the Restriction of Copyright of Visually Impaired P...

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作者： Ghani hafiz usman 中南财经政法大学

学位级别：硕士

本研究建立在先前的一些研究和论文的基础上,这些研究和论文着眼于版权和视障人士需求之间的关系,视障人士无法阅读已出版的版权作品。这项研究特别探讨在权利的例外情况下,如何在著作权人、视觉受损的版权作品使用者及协助使用者的利... 详细信息

本研究建立在先前的一些研究和论文的基础上,这些研究和论文着眼于版权和视障人士需求之间的关系,视障人士无法阅读已出版的版权作品。这项研究特别探讨在权利的例外情况下,如何在著作权人、视觉受损的版权作品使用者及协助使用者的利益之间取得适当的平衡。本文也着眼于已经确定的版权问题的其他可能解决方案。本文同时探讨了欧洲、中国和巴基斯坦的《视障人士法》(VIPs)对视障人士使用作品的版权限制以及与之相关的问题。此外,本研究通过不同的案例探讨发展中国家视障者版权在版权有效性认定和相关法律共存协议下的作用。在人权与知识产权交叉的框架下,本文研究了视障人士对增加获得受版权保护作品的需求。本研究表明,不能充分获得受版权保护的材料是对视障人士的歧视,并对他们获得教育、信息、保健、就业、文化和科学的人权产生负面影响。此外,结合《伯尔尼公约》、《与贸易有关的知识产权协定》和《世界知识产权组织互联网条约》,本文研究了国际和国家版权法对视障人士要求的影响。在对国际版权法的分析中,本文评价了《马拉喀什条约》并对巴基斯坦、中国和欧洲视障者著作权法进行了比较研究。最后分析了巴基斯坦的立法规定,指出了其立法的不足之处,并提出了具体的改进建议,最后一章将结合论文的结论提出建议。

关键词：视障人权利限制与例外国际国内著作权法

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Complications of Ziv-Aflibercept in Choroidal and Retinal Vascular Diseases

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Open Journal of Ophthalmology 2023年第2期13卷 199-207页

作者： Hussain Ahmad Khaqan usman Imtiaz Laraib Hassan Sabah Eric Hasnain muhammad Bukhsh hafiz Mubashir Farooqui Ahmad Fauzan muhammad usman Zia Department of Ophthalmology Post Graduate Medical Institute Lahore General Hospital Ameer Ud Din Medical College Lahore Pakistan Central Park Teaching Hospital Lahore Pakistan Sindh Institute of Ophthalmology and Visual Sciences Hyderabad Pakistan

Introduction: The modern ophthalmology trends are changing rapidly every day with the introduction of much newer studies and research. Numerous anti-vascular endothelial growth factors (VEGF) are utilized as the mainstay in the treatment of intraocular vascular pathologies. The rationale of this study is to add to the literature regarding the safety and efficacy profile of the ziv-aflibercept as there is insubstantial data in patients with intraocular vascular pathologies being treated with this injection with prime focus on the complications of the injection. Materials and Methods: A prospective observational study was conducted at Opthalmology Department, Lahore General Hospital, Lahore between 14 August 2018 and 23 December 2019. Patients with choroidal and retinal vascular diseases like diabetic macular edema (DME), age-related macular degeneration (AMD) and retinal vein occlusion (RVO) who had no active infection of eye and had no history of myocardial infarction or cerebrovascular accident were added in this study. Results: Best-corrected visual acuity was significantly improved at 4, 8, and 12 weeks as compared to the baseline (p Conclusion: The use of ziv-aflibercept injection via intravitreal route under aseptic conditions for choroidal and retinal vascular diseases is effective as well as safe with mild and treatable ocular side effects.

关键词： Ziv-Aflibercept Anti VEGF Choroidal Vascular Diseases Retinal Vascular Diseases

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家族性ALS动物模型神经肌肉的结构特征

家族性ALS动物模型神经肌肉的结构特征

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作者： hafiz usman Ghani 河北医科大学

学位级别：硕士

肌萎缩性侧索硬化症(ALS)是一种致命的神经退行性疾病,可出现脊髓和运动皮层中的运动神经元退化。尽管大多数ALS病例为散发性病例,但铜锌超氧化物歧化酶-1(SOD1)突变是导致10%-20%家族性ALS的原因.目前的治疗方法效果不佳,很大一部分受... 详细信息

肌萎缩性侧索硬化症(ALS)是一种致命的神经退行性疾病,可出现脊髓和运动皮层中的运动神经元退化。尽管大多数ALS病例为散发性病例,但铜锌超氧化物歧化酶-1(SOD1)突变是导致10%-20%家族性ALS的原因.目前的治疗方法效果不佳,很大一部分受损功能根本无法恢复,或者只能部分恢复,通常需要在患者生命周期内进行其他治疗.研究表明,基于不同阶段的干预策略可有效预防和控制家族性ALS.尽管确实存在一些改善这种退行性疾病影响的治疗方法,但是即便是现代医学和科学在不断的进步,其确切的发病机制仍然未明确。本研究使用从40天到120天的WT&SOD1G93A小鼠模型,旨在研究SOD1小鼠神经肌肉接头的结构。实验结果表明,在40天的小鼠中,90%的WT&SODI小鼠神经肌肉接头的结构中没有发生变性;在60天的小鼠中,没有发生微小的变性;而在90天的小鼠中,没有发生严重的变性,但在120天的小鼠中,有严重的结构扭曲和麻痹.目的:观察不同年龄组的家族性ALS神经肌肉接头的结构特征。方法:从雄性半合子携带者(B6SJL-Tg(SOD1-G93A)1Gur/J)和雌性B6SJL/F1杂种中选育雌性G93ASOD1转基因小鼠和与年龄匹配的野生型(WT同窝伴侣,两者均购自Jackson)实验室.将动物保持在湿度和温度可控的环境中,在黑暗的日程中进行12:12h光照,并提供无菌的无特定病原体的啮齿动物食物和无菌食物。结果1.以下结果表明,在40天的小鼠中WT和SOD1都存在。G93ASOD1小鼠的胫骨内部(TA)和腓肠肌(GN)的构建。图片(上图)显示了各种聚会中神经肌肉交叉口(NMJ)的数量。神经丝重多肽(微管蛋白,绿色)指向突触前末端。α-真菌毒素(BTX,白色)标记突触后间隙,突触素(p38-1,红色)用于突触小泡。以下结果表明两者均未发生退化,并且完成了90%.2.在60天的小鼠中显示WT和SOD1。G93ASOD1小鼠的胫骨前部(TA)和腓肠肌(GN)的设计。代表图片(顶部)显示了各种聚会中神经肌肉交叉口的数量。神经丝重多肽(微管蛋白,绿色)指向突触前末端.α-真菌毒素(BTX,白色)标记突触后区域,突触素(p38-1,红色)用于突触小泡。结果表明,在WT和SOD1中,变性都约为70%到80%,这意味着发生了轻微的变性。3.在90天的小鼠中同时显示了WT和SOD1。G93ASOD1小鼠的胫前肌(TA)和腓肠肌(GN)的结构。代表性图像(上图)显示了不同组中神经肌肉接头(NMJ)的数量。神经丝重多肽(微管蛋白,绿色)代表突触前的末端。标记突触后结构域的α-真菌毒素(BTX,白色),突触小泡(p38-1,红色)用于突触小泡。来自WT和SOD1的数据显示,在90天的小鼠中,变性并不严重,大约60%至70%。4.在120天的小鼠中同时显示WT和SOD1。G93ASOD1小鼠的胫前肌(TA)和腓肠肌(GN)的结构。代表性图像(顶部)显示了不同组中神经肌肉接头(NMJ)的数量。神经丝重多肽(微管蛋白,绿色)代表突触前的末端。标记突触后结构域的α-真菌毒素(BTX,白色),突触小泡(p38-1,红色)用于突触小泡。来自WT和SOD1的数据显示,在120天的小鼠中,其结构发生了严重变形,并发生了瘫痪.结论:在这项研究中,在观察40天、60天、90天及120天的WT和SOD1小鼠神经肌肉接头的结构特征后,我们得出结论:在40天时,已出现NMJ的神经支配消失,失去运动轴突,在60天及90天时出现了NMJ巨大的结构性损伤。根据我们的数据,我们观察到突触前和突触后结构变化以及突触小泡逐渐减少。应当进一步研究以探索ALS的复杂系统表型。在本研究中,我们确定了这些系统性改变的基础分子事件,为其作为药物靶标的可行性提供了实验证据。

关键词：肌萎缩侧索硬化神经肌肉接头突触

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Effect of Nitrogen on Yield and Oil Quality of Sunflower (<i>Helianthus Annuus</i>L.) Hybrids under Sub Humid Conditions of Pakistan

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American Journal of Plant Sciences 2012年第2期3卷 243-251页

作者： Wajid Nasim Ashfaq Ahmad Asghari Bano Rabiu Olatinwo muhammad usman Tasneem Khaliq Aftab Wajid hafiz Mohkum Hammad muhammad Mubeen Muzzammil Hussain Adaptive Research Farm Government of Punjab Lahore Pakistan Agro-Climatology Laboratory Department of Agronomy University of Agriculture Faisalabad Pakistan Department of Biological & Agricultural Engineering University of Georgia Griffin USA Department of Irrigation & Drainage University of Agriculture Faisalabad Pakistan Department of Plant Sciences Quaid-i-Azam University Islamabad Pakistan

Sunflower (Helianthus annuus L.) has emerged as an economically important crop in Pakistan due to its significant share in vegetable oil production. The plant metabolic processes require protein to increase the vegetative, reproductive growth and yield of the crop. The protein is wholly dependent upon the amount of nitrogen fertilization available for plant use. A two-year field study was conducted in 2008 and 2009. The objective was to determine the effect of different nitrogen (N) levels (N1 = 0 kg·ha–1, N2 = 60 kg·ha–1, N3 = 120 kg·ha–1, N4 = 180 kg·ha–1 and N5 = 240 kg·ha–1) on three sunflower hybrids (Hysun-33, Hysun-38 and Poineer-64A93) in agro-climatic conditions of Gujranwala, a sub-humid region in the centre of the Punjab province of Pakistan. A randomized complete block design split plot experiment was set-up with cultivars in the main plots and N levels in the subplots. Results showed that Hysun-38 gave maximum TDM (15815 kg·ha–1) and maximum grain yield (3389 kg·ha–1), while minimum TDM (14640 kg·ha–1) and grain yield (3125 kg·ha–1) was observed in Hysun-33. Among different N rates evaluated, N4 gave maximum TDM (17890 kg·ha–1) and grain yield (3809 kg·ha–1) compared to the other N rates. The maximum oil content (46.2%) was observed in Hysun-38 without application of N fertilizer (N1), while the minimum oil content (40.6%) was observed from N5 treatment. In conclusion, the application of 180 kg·ha–1 N to Hysun-38 provided the best combination for good yield in sunflower crop under the prevailing sub-humid conditions of Pakistan.

关键词： Helianthus annuus L. Nitrogen Rates Yield Subhumid Conditions Pakistan

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Gene Action Studies in the Inheritance of Economic Traits in Diallel Cross of Lentil (Lens culinaris Medik)

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American Journal of Plant Sciences 2016年第6期7卷 756-768页

作者： Fahim Abbas Asim Riaz Tanzeela Zafar muhammad Hassan hafiz muhammad Imran Umar Javed Hassan Waseem Alam Saleh muhammad Mudassir Mahmood Hamza Sohail usman Ali Tiwana muhammad Tayyab Iqbal muhammad Bilal Nanjing Agriculture University Nanjing China Department of Pharmacy Health and Well-Being University of Sunderland Sunderland UK Centre of Agricultural Biochemistry and Biotechnology Faculty of Agriculture University of Agriculture Faisalabad Pakistan Department of Plant Breeding & Genetics Bahauddin Zakariya University Multan Pakistan Department of Entomology Bahauddin Zakariya University Multan Pakistan Department of Horticulture Bahauddin Zakariya University Multan Pakistan Arid Zone Research Institute Bahawalpur Pakistan Department of Plant Breeding & Genetics PMAS-UAA Rawalpindi Pakistan

The present study was conducted in 4 × 4 half diallel cross fashion using the genotypes named as NL-2002, NL-2006, Punjab Masoor-2009 and Markaz-2009 for the evaluation of gene action in the inheritance of economic traits of lentil by following the Griffing (1956) Method of analysis. Minirap 13.1 Copyright© 2000, Minitab inc. Method II, Model I was used for the analysis and gene action was computed by variance component analysis using Mixed Model with GLM (general linear model) procedure. The F1 direct crosses along with the parental genotypes were then evaluated by framing the experiment in RCBD in 3 replications. The data were recorded for the characters which are under the study are, plant height (cm), number of primary branches, number of secondary branches, number of pods per plant, number of seed per pod, seed yield per plant, biomass (gram), 100 seeds weight (gram), harvest index (%), yield per plant. The simple ANOVA for all the characters revealed that difference among genotypes was highly significant. As significant differences were present between the genotypes, it permitted subsequent analysis of the data. The Combining Ability (CA) results showed that value of variance for GCA (General Combining Ability) was significantly higher than that of those of the values of variance of SCA (Specific Combining Ability). Hence current results clearly depicted the role of additive type of gene action governing the characters under study.

关键词： Lentil Diallel Gene Action Studies

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DNA Extraction from Insects by Using Different Techniques: A Review

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Advances in Entomology 2015年第4期3卷 132-138页

作者： usman Asghar muhammad Faheem Malik Fakhra Anwar Ayesha Javed Ali Raza Department of Zoology University of Gujrat Hafiz Hayat Campus Gujrat Pakistan

This review paper was prepared at Department of Zoology, University of Gujrat, Pakistan during 2014-2015. The data for the two decades, DNA Extraction from Insects by Using Different Techniques was compiled through a thorough review of many research articles published in various journals of international repute. Insects are very important economically and ecologically for human due to their various roles, as pests, decomposer in nutrition cycle, vector for the transmission of certain diseases and a source of medically and economically important products. To identify insect, and for research on their role, different molecular techniques are used. For each molecular technique, high quality DNA is required, which is extracted from the insects by different techniques. The selection of DNA extraction technique is depended upon specimen under study, time required for extraction, economical stander of technique due to reagents and equipment used for extraction and most importantly extracted DNA quality. In this paper, most frequently used techniques for DNA extraction from insect with different size and condition are outlined. This paper is only a guide for these techniques and we describe them briefly.

关键词： Extraction Techniques Reagents

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