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Phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos

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Asian Pacific Journal of Tropical Biomedicine 2018年第11期8卷 554-564页

作者： Zalilawati Mat Rashid Abdul M. Ali Philippe Douzenel Nathalie Bourgougnon Khozirah Shaari Yosie Andriani Tengku Sifzizul Tengku Muhammad habsah mohamad Faculty of Bioresources and Food Industry Universiti Sultan Zainal Abidin Besut Campus School of Agriculture Science & Biotechnology Universiti Sultan Zainal Abidin Besut Campus Faculté des Sciences et Sciences de l'Ingénieur (UFR SSI) Centre Yves Coppens Université Européenne de Bretagne Campus de Tohannic - BP 573 LBCM Université Européenne de Bretagne Campus de Tohannic BP573 Laboratory of Natural Products Institute of Bioscience Universiti Putra Malaysia Institute of Marine Biotechnology University Malaysia Terengganu Mengabang Telipot

Objective: To investigate phenolics, fatty acids composition and biological activities of various extracts and fractions of Malaysian Aaptos aaptos. Methods: Fatty acid methyl ester was analyzed by gas chromatography-flame ionization detector. Antioxidant activity was determined using 2,2-diphenyl-picrylhydrazyl radical scavenging assay and total phenolics content by Folin-Ciocalteu procedure. Vero cells viability was evaluated using methyl thiazole tetrazolium and the inactivation of herpes simplex virus type 1 by neutral red uptake assay. p-Hydroxybenzamide isolated by column chromatography was characterized by utilizing nuclear magnetic resonance spectroscopy and electron impact mass spectrometry. Results: The chloroform, ethyl acetate and methanol extracts of Aaptos aaptos produced higher portions of straight-chain saturated fatty acid, while hexane extract mainly consisted of unsaturated fatty acid. The five majors of fatty acid methyl ester were identified as behenic acid, cis-10-heptadecenoic acid and cis-10-pentadecenoic acids, palmitic acid and tricosanoic acid. In addition, among all organic extracts, chloroform extract inactivated herpes simplex virus type 1 while exhibited weak cytotoxic activity against normal Vero cells and also exhibited strong cytotoxic activity on HL-60, MCF-7, K562, CEM-SS and WEHI-3 B cells. A phenolic compound, p-hydroxybenzamide was also isolated from the sponge. Conclusions: Aaptos aaptos could be a source to derive the potential antiviral and anticancer agents. However, further studies are needed to determine the mechanism involved in the process.

关键词： Aaptos aaptos Fatty acid p-Hydroxybenzamide Antioxidant Cytotoxicity Antivirus

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Review on Zinc Oxide Nanoparticles: Antibacterial Activity and Toxicity Mechanism

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Nano-Micro Letters 2015年第3期7卷 219-242页

作者： Amna Sirelkhatim Shahrom Mahmud Azman Seeni Noor Haida mohamad Kaus Ling Chuo Ann Siti Khadijah Mohd Bakhori habsah Hasan Dasmawati mohamad Nano-Optoelectronics Research and Technology Laboratory (N.O.R.Lab) School of Physics Universiti Sains Malaysia Advanced Medical and Dental Institute Cluster of Integrative Medicine Universiti Sains Malaysia School of Chemical Sciences Universiti Sains Malaysia Nano-Optoelectronics Research and Technology Laboratory(N.O.R.Lab) School of Physics Universiti Sains Malaysia Department of Medical Microbiology Parasitology and Immunology School of Medical Sciences Universiti Sains Malaysia School of Dental Sciences Universiti Sains Malaysia

Antibacterial activity of zinc oxide nanoparticles(Zn O-NPs) has received significant interest worldwide particularly by the implementation of nanotechnology to synthesize particles in the nanometer region. Many microorganisms exist in the range from hundreds of nanometers to tens of micrometers. Zn O-NPs exhibit attractive antibacterial properties due to increased specific surface area as the reduced particle size leading to enhanced particle surface reactivity. Zn O is a bio-safe material that possesses photo-oxidizing and photocatalysis impacts on chemical and biological species. This review covered Zn O-NPs antibacterial activity including testing methods, impact of UV illumination, Zn O particle properties(size, concentration, morphology, and defects), particle surface modification, and minimum inhibitory concentration. Particular emphasize was given to bactericidal and bacteriostatic mechanisms with focus on generation of reactive oxygen species(ROS) including hydrogen peroxide(H2O2), OH-(hydroxyl radicals), and O2-2(peroxide). ROS has been a major factor for several mechanisms including cell wall damage due to Zn O-localized interaction, enhanced membrane permeability, internalization of NPs due to loss of proton motive force and uptake of toxic dissolved zinc *** have led to mitochondria weakness, intracellular outflow, and release in gene expression of oxidative stress which caused eventual cell growth inhibition and cell death. In some cases, enhanced antibacterial activity can be attributed to surface defects on Zn O abrasive surface texture. One functional application of the Zn O antibacterial bioactivity was discussed in food packaging industry where Zn O-NPs are used as an antibacterial agent toward foodborne diseases. Proper incorporation of Zn O-NPs into packaging materials can cause interaction with foodborne pathogens, thereby releasing NPs onto food surface where they come in contact with bad bacteria and cause the bacterial death and/or in

关键词： Antibacterial activity Zn O-NPs Toxicity mechanism Reactive oxygen species Zinc ions release Food antimicrobial

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The Conservation of Ground Water Levels in the Peat Swamp Forest at Ayer Hitam North Forest Reserve, Muar in Johor, Malaysia

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Journal of Water Resource and Protection 2021年第12期13卷 957-975页

作者： Siti Aisah Shamsuddin Mohd Azahari Faidi Hyrul Izwan Mohd Husin mohamad Fakhri Ishak Ismail Parlan habsah Marjuni Forestry & Environment Division Forest Research Institute Malaysia Kepong Selangor Malaysia Forest Biotechnology Division Forest Research Institute Malaysia Kepong Selangor Malaysia Director General Forest Research Institute Malaysia Kepong Selangor Malaysia Yayasan Kemanusiaan Muslim Aid Malaysia Menara Mutiara Bangsar Jalan Liku Off Jalan Riong Kuala Lumpur Malaysia

The management of peat swamp forests in Malaysia contends with two major issues: forest fires and the effects of abandoned forest-logging drainage systems or canals. Forest fire occurs during low rainfall season related to the local people activities. The drainage networks change the hydrological function of the intact forest ecosystem. A key function of the hydrological system in the undisturbed forest is to absorb water during rainfall season, thus delaying downstream runoff and preventing flash floods. The objective of the project described here is to restore the hydrological function of peat swamp forest (PSF) at Ayer Hitam North Forest Reserve (AHNFR) in Muar, Johor, Malaysia. The oil palm plantations, especially in the southern part of the area affect the forest reserve. Water flows out of the forest reserve through the drainage system constructed for managing these plantations. In 2016 and 2017, two water block structures or check dams were constructed near the boundaries of the forest reserve to hold the water and raise the groundwater level in the forest reserve. The implementation of the check dams at the two locations has conserved the groundwater level and subsequently, about 1.2 million m3 of water was saved annually from leaving the forest reserve from each of the check dam. This project is also part of the Coca-Cola Company’s sustainability commitment for water strategy with the global that is to replenish 100% of the equivalent volume of water consumed in their products and production by 2020. Replenishment is the key sustainability commitment for the Company.

关键词： Hydrology Oil Palm Plantation Water Blockage Peat Swamp Forest Ground Water Level Conservation

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The Conservation of Ground Water Levels in the Peat Swamp Forest at Ayer Hitam North Forest Reserve, Muar in Johor, Malaysia

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Journal of Water Resource and Protection（水资源与保护） 2021年第12期

作者： Siti Aisah Shamsuddin Mohd Azahari Faidi Hyrul Izwan Mohd Husin mohamad Fakhri Ishak Ismail Parlan habsah Marjuni

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