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MACS-annexin V cell sorting of semen samples with high TUNEL values decreases the concentration of cells with abnormal chromosomal content:a pilot study

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Asian Journal of Andrology 2022年第5期24卷 445-450页

作者： Sahar El Fekih Nadia Gueganic Corinne Tous habib ben ali Mounir Ajina Nathalie Douet-Guilbert Hortense Drapier Damien Beauvillard Frédéric Morel Aurore Perrin University of Brest InsermUMR U1078Faculty of Medicine and Health SciencesBrest 29238France Laboratory of Human Cytogenetics Molecular Genetics and Reproductive BiologyFarhat Hached University HospitalSousse 4031Tunisia Departement of Medical Genetics and Reproductive Biology Brest University Regional HospitalBrest 29609France Department of Reproductive Medicine Farhat Hached Teaching HospitalSousse 4031Tunisia

We question whether,in men with an abnormal rate of sperm DNA fragmentation,the magnetic-activated cell sorting(MACS)could select spermatozoa with lower rates of DNA fragmentation as well as spermatozoa with unbalanced chromosome *** spermatozoa from six males were separated into nonapoptotic and apoptotic *** determined the percentages of spermatozoa with(i)externalization of phosphatidylserine(EPS)by annexin V-Fluorescein isothiocyanate(FITC)labeling,(ii)DNA fragmentation by TdT-mediated-dUTP nick-end labeling(TUNEL),and(iii)numerical abnormalities for chromosomes X,Y,13,18,and 21 by fluorescence in situ hybridization(FISH),on the whole ejaculate and selected spermatozoa in the same *** to the nonapoptotic fraction,the apoptotic fraction statistically showed a higher number of spermatozoa with EPS,with DNA fragmentation,and with numerical chromosomal *** to the whole ejaculate,we found a significant decrease in the percentage of spermatozoa with EPS and decrease tendencies of the DNA fragmentation rate and the sum of disomy levels in the nonapoptotic ***,we observed statistically significant higher rates of these three parameters in the apoptotic *** may help to select spermatozoa with lower rates of DNA fragmentation and unbalanced chromosome content in men with abnormal rates of sperm DNA fragmentation.

关键词： chromosomal abnormalities DNA fragmentation externalization of phosphatidylserine magnetic-activated cell separation spermatozoa

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Oxidative stress in male infertility and therapeutic approach: A mini-review

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Asian pacific Journal of Reproduction 2023年第6期12卷 249-255页

作者： Marwa Lahimer Henda Mustapha Véronique Bach Hafida Khorsi-Cauet Moncef benkhalifa Mounir Ajina habib ben ali Service of Reproductive Medicine University Hospital Farhat HachedSousseTunisia Exercise Physiology and Physiopathology:from Integrated to Molecular“Biology Medicine and Health”(Code:LR19ES09)SousseTunisia Péritox(UMR_I 01) UPJV/INERISUPJVCURSChemin de Thil 80025 AmiensFrance ART and Reproductive Biology Laboratory University Hospital and School of MedicinePicardie University Jules VerneCHU Sud80054AmiensFrance Laboratory Histology Embryology Faculty of Medicine SousseUniversity of SousseSousseTunisia

Growing evidence suggests that oxidative stress is a major cause of male *** are highly sensitive cells due to the vulnerability of their membrane fatty acids and the diminished antioxidant *** oxygen species(ROS)impact multiple signaling pathways involved in mitochondrial dysfunction,hormonal unbalance and semen quality *** origin of ROS is diverse,including generated normal cellular metabolism,environmental exposure,advanced paternal age and *** has indicated that antioxidant supplementation can improve oxidative stress and reduce the risk of chronic ***,it is widely known that antioxidant supplementation can enhance sperm quality and increase the pregnancy rate in couples undergoing fertility *** current study highlights the deleterious effect of ROS and its impact on semen *** addition,it contributes to the development of a clinical approach for the use of antioxidants in the management of male infertility.

关键词： Oxidative stress Reactive oxygen species Mitochondria Antioxidant supplementation Spermatozoa

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Study of gonadic growth factors: Seminal transforming growth factor-β1, epidermal growth factor and insulin-like growth factor-I and their relationship with male infertility

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Advances in Reproductive Sciences 2014年第1期2卷 16-23页

作者： Houda Amor Mounir Ajina ali Saad habib ben ali Laboratoire de Cytogénétique Génétique Moléculaire et Biologie de la Reproduction Humaines CHU Farhat Hached Sousse Tunisie Unité de Médecine de la Reproduction CHU Farhat Hached Sousse Tunisie

Background: The aim of this study was to establish normal reference values for several growth factors in semen and provide a basis for understanding their physiological significance in fertile and infertile subjects. Methods: 110 infertile men semen was evaluated according to WHO standards and by enzyme immunoassay (ELISA kit protocol) to determine the rate of growth factors (TGFβ1, IGFI, EGF). We have also listed neutrophils by staining with peroxidase to examine the association of inflammation with the levels of these factors. The determination of the epididymal (alpha-1,4-glucosidase) marker was directed according to the technical recommended by the WHO in 2010. Each patient received a hormonal balance including the determination of plasma FSH, LH and Testosterone. Results: Concentrations of TGFβ1 were significantly higher in (severe and extreme azoospermic, oligospermic) pathological groups compared to the control group (P 0.05) between control and pathological groups. The IGFI is partly testicular and/or epididymal as it is lowered in some patients with obstructive azoospermia. Positive correlations were observed between the seminal IGFI, mobility (r = 0.487) and sperm morphology (r = +0.187). Immunological sperm abnormalities and/or idiopathic do not alter the rate of EGF seminal. There is no significant correlation between seminal EGF or with sperm parameters or with plasma levels of FSH and LH. Conclusion: The growth factors are primarily peptides, handling and identification in a complex environment as the seminal plasma, but it requires a lot of precautions if we do not want to expose to the risk of bad farms/interpretations of data obtained.

关键词： Infertility Semen EGF IGF-I TGF-β1 FSH LH

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Impact of the professional exposure on the spermatic parameters and the results of ICSI at Tunisian unfertile couples

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Advances in Reproductive Sciences 2014年第1期2卷 24-31页

作者： habib ben ali Monia Zaouali Fatma Atig Iheb Bougmiza Souhir Mehri Houda ben Frej Faten Tabka ali Saad Mounir Ajina Laboratoire de Cytogénétique Génétique Moléculaire et Biologie de la Reproduction Humaines CHU Farhat Hached de Sousse Sousse Tunisie Laboratoire de Physiologie et des Explorations Fonctionnelles Faculté de Médecine de Sousse Sousse Tunisie Service de Médecine Communautaire Faculté de Médecine de Sousse Sousse Tunisie Service de Médecine de Travail CHU Farhat Hached de Sousse Sousse Tunisie Unité de Médecine de la Reproduction CHU Farhat Hached de Sousse Sousse Tunisie

Introduction: The association of the professional exposure to the decline of the male reproductive function had been the subject of several studies but without arriving to one envisagement undeniable of its existence. The ICSI, technique of reference in severe male infertilities, is visavis unexplained failures whose environmental and professional exposure could be about its cause partly. In this work, we tried to highlight an association between certain factors of professional exposure and the deterioration of the spermatic parameters, then to evaluate the effect of such factors on the result of the ICSI. Materials and Methods: It is about a study of the exposed type—no exposed, realized through collaboration between the Unit of Medicine of the Reproduction and the Service of Medicine of Work of the CHU Farhat Hached of Sousse. This study was based on a questionnaire of which part of the items was collected medical files of the patients raising the general characteristics, the profile of exposure as well as the spermatic parameters and the biological assessment of ICSI. Results: Significant associations between the deterioration of sperm and the professional risk factors such as the exposure to the formaldehydes (p = 0.01) and heat (p = 0.001) were noted. The exposure to formaldehydes was associated at the higher risk of oli gozoospermy, of asthenozoospermy with a significant reduction in mobility (p = 0.05), whereas the talks with cement had a risk higher of teratozoospermy. In addition an exposure to heat was associated at the high risk of oligoteratozoospermy. The total rate of pregnancies after ICSI among 220 patients of our series was of 14.47%. The ICSI balance showed a reduction in total fertility rate (76.6% exposed vs 75.3% no exposed). Conclusion: In spite of the limited number of the patients, this study had shown a correlation between certain professional exposures of origin and the deterioration of sperm. They are preliminary results which encourage co

关键词： Male Sterility Professional Exposure Spermogramm ICSI

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beneficial Effect of an Oral Antioxidant Supplementation (Fertimax2) on IVF-ICSI Outcomes: A Preliminary Clinical Study

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Advances in Reproductive Sciences 2014年第3期2卷 47-56页

作者： Olfa Kacem Meriem Harzallah Chekib Zedini Ines Zidi Sawsen Meddeb Meriem Fékih habib Saidi Anouar Chaib Sassi Boughizane habib ben ali Mohamad Bibi ali Saad Hédi Khairi Mounir Ajina Department of Obstetrics and Gynaecology University Hospital F. Hached Sousse Tunisia Laboratories of Cytogenetic Molecular Biology and Human Biology of Reproduction F Hached Hospital Sousse Tunisia Service of Community Medicine Faculty of Medicine Sousse Tunisia Unit of Reproductive Medicine University Hospital F Hached Sousse Tunisia

Background: Considerable evidence points towards a significant role of oxidative stress (OS) in the pathogenesis of sperm dysfunction. OS as a result of an inappropriate balance between oxidants and antioxidants in the semen can cause DNA damage and lipid peroxidation leading to failure of conception, miscarriage or potentially even childhood cancer. The objective of this study was to investigate whether a male antioxidant therapy can improve semen parameters and the results of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Methods: A total of forty eight infertile couples were considered. Male participants were administrated Fertimax2 antioxidant treatment for at least two months prior to their partner’s IVF-ICSI cycle. Sperm parameters (volume, concentration, progressive motility) and the IVF-ICSI outcomes were compared before and after the antioxidant treatment. The primary outcome measures were oocyte fertilization, cleavage and good embryo quality rates;the secondary outcomes were biochemical pregnancies, clinical pregnancies and implantation rates. Results: The principal finding that emerged from this study was that antioxidant therapy resulted in significant improvements in fertilization (p = 00.2), cleavage (p = 0.004) and good-embryo quality (p = 0.002) rates accompanied by a marked increase in clinical pregnancy (18.7% versus 2.5%) and implantation (11.8% versus 1.02%) rates. No significant changes in routine sperm parameters were observed. Conclusion: The Fertimax2 antioxidant therapy appears to influence favorably chances of conception in couples undergoing assisted reproduction treatment (ART).

关键词： Sperm Oxidative Stress Antioxidants Infertility Treatment IVF-ICS

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Cooperative Answering of Fuzzy Queries

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Journal of Computer Science & Technology 2009年第4期24卷 675-686页

作者： Narjes Hachani Mohamed ali ben Hassine Hanene Chettaoui habib Ounelli Department of Computer Sciences Faculty of Sciences of Tunis University Campus Tunis 1060 Tunisia

The majority of existing information systems deals with crisp data through crisp database systems. Traditional Database Management Systems （DBMS） have not taken into account imprecision so one can say there is some sort of lack of flexibility. The reason is that queries retrieve only elements which precisely match to the given Boolean query. That is, an element belongs to the result if the query is true for this element; otherwise, no answers are returned to the user. The aim of this paper is to present a cooperative approach to handling empty answers of fuzzy conjunctive queries by referring to the Formal Concept Analysis （FCA） theory and fuzzy logic. We present an architecture which combines FCA and databases. The processing of fuzzy queries allows detecting the minimal reasons of empty answers. We also use concept lattice in order to provide the user with the nearest answers in the case of a query failure.

关键词： cooperative system database empty answer formal concept analysis fuzzy query

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SCALAR CURVATURE TYPE PROBLEM ON THE THREE DIMENSIONAL BOUNDED DOMAIN

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Acta Mathematica Scientia 2017年第1期37卷 139-173页

作者： Mohamed ben AYED habib FOURTI Faculte des Sciences Universite de SfaxRoute SoukraSfaxTunisie Institut Preparatoire Aux Etudes d'Ingenieurs Universite de SfaxSfaxTunisie

In this paper we prove an existence result for the nonlinear elliptic problem：-△u = Ku5,u 〉 0 in Ω,u = 0 on Ω,where Ω is a smooth bounded domain of R3 and K is a positive function in Ω.Our method relies on stud... 详细信息

关键词： scalar-curvature critical point limiting Sobolev exponent variational prob-lems with lack of compactness blow up analysis

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Numerical investigation of velocity ratio effect in combined wall and offset jet flows

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Journal of Hydrodynamics 2018年第6期30卷 1105-1119页

作者： Nidhal Hnaien Salwa Marzouk habib ben Aissia Jacques Jay Unité de Métrologie et des systèmes énergétiques Ecole National d'ingénieurs de Monastir Tunisie Centre de Thermique de Lyon (CETHIL-UMR CNRS 5008) INSA de Lyon France

In the present work, we propose to numerically study a combined turbulent wall and offset jet flow(this combination will be denoted WOJ). Several turbulence models were tested in this study: the standard k-w, SST k-w, standard k-ε, RNG, and realizable k-ε model. A parametric study was performed to determine the effect of offset ratio H and the velocity ratio r on the longitudinal and transverse positions of the merge point(MP), the combined point(CP), the upper vortex center(UVC) and the lower vortex center(LVC). Correlations that predict the position of these characteristic points of the WOJ flow as a function of H and r have been provided. Results show that any increase in the velocity ratio causes a displacement of the MP, CP, UVC and LVC further upstream along the longitudinal direction. Concerning the transverse positions, the increase of velocity ratio results in a deviation of the merge point(MP) and the lower vortex center(LVC) toward the strong jet(LWJ) whereas the transverse position of combined point(CP) and the upper vortex center(UVC) is almost independent of the velocity ratio.

关键词： Finite volume combined point merge point offset jet velocity ratio

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Metals and metalloid bioconcentrations in the tissues of Typha latifolia grown in the four interconnected ponds of a domestic landfill site

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Journal of Environmental Sciences 2017年第4期29卷 56-68页

作者： Zohra ben Salem Xavier Laffray Ahmed Al-Ashoor habib Ayadi Lotfi Aleya Bourgogne Franche-Comte University Chrono-Environnement Laboratory UMR CNRS 6249 F-25030 Besancon Cedex France Paris Est-Creteil University IPE team iEES Paris UMR 7618 F-94010 Creteil Cedex Prance Thi Qar University IQ-64001 AI Nasiriyah Iraq Sfax University LR/UR/05ES05 Biodiversity and Aquatic Ecosystem BP 1171 CP 3000 Sfax Tunisia

The uptake of metals in roots and their transfer to rhizomes and above-ground plant parts（stems, leaves） of cattails（Typha latifolia L.） were studied in leachates from a domestic landfill site（Etueffont, France） and treated in a natural lagooning system. Plant parts and corresponding water and sediment samples were taken at the inflow and outflow points of the four ponds at the beginning and at the end of the growing season. Concentrations of As, Cd, Cr, Cu, Fe, Mn, Ni and Zn in the different compartments were estimated and their removal efficiency assessed, reaching more than 90% for Fe, Mn and Ni in spring and fall as well in the water compartment. The above-and below-ground cattail biomass varied from 0.21 to 0.85, and 0.34 to 1.24 kg dry weight/m^2, respectively, the highest values being recorded in the fourth pond in spring 2011. The root system was the first site of accumulation before the rhizome, stem and leaves. The highest metal concentration was observed in roots from cattails growing at the inflow of the system＇s first pond. The trend in the average trace element concentrations in the cattail plant organs can generally be expressed as： Fe 〉 Mn 〉 As 〉 Zn 〉 Cr 〉 Cu 〉 Ni 〉 Cd for both spring and fall. While T. latifolia removes trace elements efficiently from landfill leachates, attention should also be paid to the negative effects of these elements on plant growth.

关键词： Landfill leachate Lagooning Trace elements Typha latifolia Phytoremediation

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Relationship between high sensitivity C-reactive protein and angiographic severity of coronary artery disease

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Journal of Geriatric Cardiology 2020年第5期17卷 256-263页

作者： Nadia Bouzidi Mejdi ben Messaoud Faouzi Maatouk habib Gamra Salima Ferchichi University of Monastir Faculty of PharmacyClinical and Molecular Biology UnitMonastirTunisia University of Monastir Cardiology A Department Fattouma Bourguiba University HospitalCardiothrombosis Research LaboratoryTunisia University of Monastir Cardiology B Department Fattouma Bourguiba University HospitalCardiothrombosis Research LaboratoryTunisia

Background Coronary artery disease(CAD)remains a leading cause of morbidity and *** play a potential role in atherosclerosis pathogenesis and *** investigated the association between high sensitive C-reactive protein(hs CRP)and severity of *** CAD patients were stratified according to hs CRP cut-off value into high levels hs CRP group(≥8.4 mg/L)and low levels hs CRP group(<8.4 mg/L).Severity of CAD was assessed according to artery stenosis degree and the number of vessel *** analysis was performed using Statistical Package for the Social Sciences(SPSS,version 23.0).Results The mean age was 60.3±11.0 *** level of hs CRP was increased and ranged from 0.2 to 1020.0 mg/*** risk factors and severity of CAD didn’t show significant differences between the two *** multivariate linear analysis,cardiac troponin I(c Tn I)and serum amyloid A(SAA)were predictors of hs *** shown in receiver operating characteristic(ROC)curve analysis performed in patients with ST-segment elevation myocardial infarction(STEMI)and compared to myonecrosis biomarkers,hs CRP(area under the curve(AUC):0.905;95%CI:0.844-0.966;P<0.001)could be a powerful predictor marker in evaluating the infarct size after myocardial infarction but not better than c Tn *** Hs CRP levels were not associated with the severity of CAD but could be useful in the evaluation of myocardial necrosis in patients with STEMI.

关键词： Coronary artery disease High sensitive C-reactive protein Severity

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