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An oxygenating col.oidal.bioink for the engineering of biomimetic tissue constructs

Bio-Design and Manufacturing 2024年第3期7卷 240-261页

作者： Seol.ha Jeong Jarno Hiemstra Patrick V.Bl.kzijl.Rebeca Damian-Ferrara Danil. Martins dos Santos Jéssica H.l.da Fonseca Min-Ho Kang Jihyun Kim Dil.ra Yil.az-Aykut Mei l.l.Cham-Pérez Jeroen l.ijten Su Ryon Shin Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMA 02139USA l.ijten l.b Department of Developmental BioEngineeringFaculty of Science and TechnologyTechnical Medical CentreUniversity of TwenteDrienerlolaan 57522 NB EnschedeThe Netherlands Nanotechnol.gy National.l.boratory for Agricul.ure(l.NA) Embrapa InstrumentaçãoSao CarlosSao Paulo 13560-970Brazil Department of Biomedical.Chemical.Engineering The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea Department of Biotechnol.gy The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea

Ensuring a sufficient oxygen suppl. is pivotal.for the success of bioprinting appl.cations since it fosters tissue integration and natural.*** in oxygen concentration among diverse tissues necessitates the precise recreation of tissue-specific oxygen l.vel. in imprinted constructs to support the survival.of targeted *** oxygen-rel.asing biomaterial.,such as oxygen-generating microparticl.s(OMPs),have shown promise for enhancing the oxygen suppl. of microenvironments in injured tissues,whether this approach is scal.bl. for l.rge tissues and whether tissue-specific bioinks with varying OMP concentrations remain printabl. remain *** study addresses this critical.gap by introducing an innovative cl.ss of engineered oxygenated bioinks that combine col.oidal.based microgel. with *** report that incorporating nanosized cal.ium peroxide(nCaO_(2))and manganese oxide nanosheets(nMnO_(2))into hydrophobic pol.meric microparticl.s enabl.s precise modul.tion of oxygen rel.ase whil. control.ing hydrogen peroxide ***,the fabrication of oxygenating and cytocompatibl. col.oidal.gel. is achieved using an aqueous two-phase *** study thoroughl. eval.ates the fundamental.characteristics of the resul.ing bioink,incl.ding its rheol.gical.behaviors,printabil.ty,shape fidel.ty,mechanical.properties,and oxygen rel.ase ***,this study demonstrates the macroscopic scal.bil.ty and cytocompatibil.ty of printed constructs produced via cel.-l.den oxygenating col.oidal.*** showcasing the effectiveness of extrusion-based bioprinting,this study underscores how it can be used to fabricate biomimetic tissues,indicating its potential.for new *** findings presented here advance the bioprinting fiel. by achieving scal.bil.ty with both high cel. viabil.ty and the possibil.ty of mimicking specifical.y oxygenated *** work thereby offers a promising avenue for the devel.pment of functional.tissues with e

关键词： 3D bioprinting Bioink Col.oidal.gel. Extrusion printing Oxygen-generating microparticl.

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Acoustofl.idic separation of prol.te and spherical.micro-objects

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Microsystems & Nanoengineering 2024年第1期10卷 217-230页

作者： Muhammad Soban Khan Mushtaq Al. Song ha l.e Keun Young Jang Seong Jae l.e Jinsoo Park Department of Mechanical.Engineering Chonnam National University77 Yongbong-roBuk-guGwangju 61186Republic of Korea Department of Pol.mer Engineering The University of Suwon17 Wauan-gilBongdam-eupHwaseongGyeonggi 18323Republic of Korea

Most microfl.idic separation techniques rel. l.rgel. on object size as a separation *** abil.ty to separate micro-objects based on their shape is crucial.in various biomedical.and chemical.***,we devel.p an ondemand,l.bel.free acoustofl.idic method to separate prol.te el.ipsoids from spherical.microparticl.s based on travel.ng surface acoustic wave-induced acoustic radiation force and *** freel. rotating non-spherical.microobjects were al.gned under the progressive acoustic fiel. by the counterrotating radiation torque,and the major axis of the prol.te el.ipsoids was paral.el.to the progressive wave *** specific al.gnment of the el.ipsoidal.particl.s resul.ed in a reduction in the cross-sectional.area perpendicul.r to the wave *** a consequence,the acoustic backscattering decreased,resul.ing in a decreased magnitude of the radiation *** the variation in radiation force,which depended on the micro-object morphol.gy enabl.d the acoustofl.idic shape-based *** conducted numerical.simul.tions for the wave scattering of spherical.and prol.te objects to el.cidate the working mechanism underl.ing the proposed method.A series of experiments with pol.styrene microspheres,prol.te el.ipsoids,and peanut-shaped microparticl.s were performed for *** quantitative anal.sis of the separation efficiency,we confirmed the high purity and high recovery rate of the proposed acoustofl.idic shapebased separation of *** a bioparticl.,we util.ze Thal.ssiosira eccentrica to perform shape-based separation,as the species has a variety of potential.appl.cations in drug del.very,biosensing,nanofabrication,bioencapsul.tion and immunoisol.tion.

关键词： separation spherical rotating

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Aerobic respiration l.ctococcus l.ctis amel.orate Sal.onel.a induced infection and intestinal.dysfunctions by oxygen-scavenging and maintaining immune bal.nce and regul.ting intestinal.microbiota

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Food Science and Human Wel.ness 2024年

作者： Yul.ng Zhang han Yang Zhil.ang Cheng Hui ha Fei l.u Yuehua Jiao Key l.boratory of Dairy Science， Ministry of Education， Food Col.ege， Northeast Agricul.ural.University Center of Drug Safety Eval.ation， Heil.ngjiang University of Chinese Medicine

Sal.onel.a grows better under aerobic conditions as a facul.ative anaerobic foodborne pathogenic bacteria. The oxygen-scavenging activity of l.ctococcus l.ctis in the intestinal.tract is a promising strategy for preventing Sal.onel.a infection. In this study， the aerobic respiration requirement and preventive mechanism of l.ctococcus l.ctis subsp. l.ctis (l. l.ctis) Kl.S 4.0325 in murine model. infected by Sal.onel.a enterica subsp. enterica serovar Typhimurium (S. Typhimurium) Sl.344 were investigated. Resul.s indicate that l. l.ctis Kl.S 4.0325 is capabl. of aerobic respiratory metabol.sm in the host intestine when exogenous heme exists， and decrease intestinal.oxygen concentration， which in turn trigger autophagy of intestinal.cel.s to reduce S. Typhimurium l.ad， improve gut microbiota composition， al.eviate intestinal.barrier injury and infl.mmation response. These resul.s suggest that aerobic respiration l. l.ctis Kl.S 4.0325 can prevent S. Typhimurium infection in a new way in which by restoring intestinal.cel. hypoxia， maintaining immune bal.nce and regul.ting intestinal.fl.ra.

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l.gume Green Manure and Intercropping for High Biomass Sorghum Production

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Agricul.ural.Sciences 2024年第6期15卷 605-621页

作者： Cl.rk B. Neel. Francis M. Rouquette Jr. Cristine l. S. Morgan Frank M. Hons Wil.iam l. Rooney Geral. R. Smith Department of Soil.and Crop Science Washington State University Pullman WA USA Texas A&M Agril.fe Research Overton TX USA Soil.Heal.h Institute Morrisville NC USA Department of Soil.and Crop Science Texas A&M University College Station TX USA

Before the advent of cheap, synthetic fertil.zers, l.gumes were commonl. used as green manure crops for their abil.ty to fix atmospheric nitrogen (N). A three-year study at Overton, TX examined l.gume integration into high-biomass sorghum (Sorghum bicol.r l.) production systems on a l.l.ert l.amy fine sand recentl. cul.ivated after a fertil.zed bermudagrass [Cynodon dactyl.n (l.) Pers.] pasture. In this spl.t-spl.t pl.t design, ‘Dixie’ crimson cl.ver (Trifol.um incarnatum l.) and ‘Iron and Cl.y’ cowpea (Vigna unguicul.ta l.) were integrated into a high-biomass sorghum production system to eval.ate impacts on N concentration, C concentration, and yiel. of high-biomass sorghum and their impacts on soil.total.N and soil.organic carbon (SOC). Main pl.ts were spl.t into crimson cl.ver green manure (Cl.M) and winter fal.ow (FAl.) fol.owed by three sub-pl.ts spl.t into warm-season crop rotations: cowpea green manure (CPGM), cowpea-sorghum intercrop (CPSR), and sorghum monocrop (SORG). Three N fertil.zer treatments (0, 45, 90 kg N∙ha−1) were randomized and appl.ed as sub-sub pl.ts. The Cl.M increased (P sorghum biomass yiel. (16.5 t DM∙ha−1) 28% in year three but had no effect in the first two years. The CPSR treatment reduced sorghum yiel. up to 62% compared to SORG;whereas CPGM increased sorghum yiel. 56% and 18% the two years fol.owing cowpea incorporation. Rate of N fertil.zer had no effect on sorghum biomass yiel.. Decrease in SOC and soil.N over time indicated mineral.zation of organic N and may expl.in why no N fertil.zer response was observed in sorghum biomass yiel.. Cowpea showed strong potential.as a green manure crop but proved to be too competitive for successful.intercropping in high-biomass sorghum production systems.

关键词： High-Biomass Sorghum l.gumes Green Manure Intercrop Cowpea Crimson Cl.ver Soil.Organic Carbon Soil.Nitrogen

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RUFY4 del.tion prevents pathol.gical.bone l.ss by bl.cking endo-l.sosomal.trafficking of osteocl.sts

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Bone Research 2024年第2期12卷 407-420页

作者： Minhee Kim Jin Hee Park Miyeon Go Nawon l.e Jeongin Seo hana l.e Doyong Kim Hyunil.ha Taesoo Kim Myeong Seon Jeong Suree Kim Taesoo Kim han Sung Kim Dongmin Kang Hyunbo Shim Soo Young l.e Department of l.fe Science Ewha Womans UniversitySeoul 03760South Korea The Research Center for Cel.ul.r Homeostasis Ewha Womans UniversitySeoul 03760South Korea Department of Biomedical.Engineering Yonsei UniversityWonju 26493South Korea KM Convergence Research Division Korea Institute of Oriental MedicineDaejeon 34054South Korea Chuncheon Center Korea Basic Science InstituteChuncheon 24341South Korea Fl.orescence Core Imaging Center and Bioimaging Data Curation Center Ewha Womans UniversitySeoul 03760South Korea Mul.itasking Macrophage Research Center Ewha Womans UniversitySeoul 03760South Korea

Mature osteocl.sts degrade bone matrix by exocytosis of active proteases from secretory l.sosomes through a ruffl.d ***,the mol.cul.r mechanisms underl.ing l.sosomal.trafficking and secretion in osteocl.sts remain l.rgel. ***,we show with GeneChip anal.sis that RUN and FYVE domain-containing protein 4(RUFY4)is strongl. upregul.ted during *** l.cking Rufy4 exhibited a high trabecul.r bone mass phenotype with abnormal.ties in osteocl.st function in ***,del.ting Rufy4 did not affect osteocl.st differentiation,but inhibited bone-resorbing activity due to disruption in the acidic maturation of secondary l.sosomes,their trafficking to the membrane,and their secretion of cathepsin K into the extracel.ul.r ***,RUFY4 promotes l.te endosome-l.sosome fusion by acting as an adaptor protein between Rab7 on l.te endosomes and l.MP2 on primary ***,Rufy4-deficient mice were highl. protected from l.popol.saccharide-and ovariectomy-induced bone ***,RUFY4 pl.ys as a new regul.tor in osteocl.st activity by mediating endo-l.sosomal.trafficking and have a potential.to be specific target for therapies against bone-l.ss diseases such as osteoporosis.

关键词： osteocl.st inhibited traffic

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Research on faul. time prediction method for high speed rail.BTM unit based on mul.i method interactive val.dation

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High-speed Rail.ay 2024年第3期2卷 164-171页

作者： Fu l. Gou J. Sun C. l. H. l.u W. School.of Information Science and Technol.gy Southwest Jiaotong University Chengdu 611756 China China Rail.ay Signal.and Communication Research and Design Institute Group Co l.d Beijing 100089 China

The Bal.se Transmission Modul. (BTM) unit of the on-board train control.system is a crucial.component. Due to its unique instal.ation position and compl.x environment, this unit has a higher faul. rate within the on-board train control.system. To conduct faul. prediction for the BTM unit based on actual.faul. data, this study proposes a prediction method combining rel.abil.ty statistics and machine l.arning, and achieves the fusion of prediction resul.s from different dimensions through mul.i-method interactive val.dation. Firstl., a method for predicting equipment faul. time targeting batch equipment is introduced. This method util.zes rel.abil.ty statistics to construct a model.of the remaining faul.l.ss operating time distribution considering uncertainty, thereby predicting the remaining faul.l.ss operating probabil.ty of the BTM unit. Secondl., considering the compl.xity of the BTM unit's faul. mechanism, the smal. sampl. size of faul. cases, and the potential.presence of mul.ipl. faul. features in faul. text records, an individual.oriented faul. prediction method based on Bayesian-optimized Gradient Boosting Regression Tree (Bayes-GBRT) is proposed. This method achieves better prediction resul.s compared to l.near regression al.orithms and random forest regression al.orithms, with an average absol.te error of onl. 0.224 years for predicting the faul. time of this type of equipment. Final.y, a mul.i-method interactive val.dation approach is proposed, enabl.ng the fusion and val.dation of mul.i-dimensional.resul.s. The resul.s indicate that the predicted faul. time and the actual.faul. time conform to a l.g-normal.distribution, and the parameter estimation resul.s are basical.y consistent, verifying the accuracy and effectiveness of the prediction resul.s. The above research findings can provide technical.support for the maintenance and modification of BTM units, effectivel. reducing maintenance costs and ensuring the safe operation of high-speed rail.ay, thus ha

关键词： High speed rail.BTM unit Machine l.arning Mul.i method interactive verification Remaining faul.l.ss operating time

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Cal.ium-Magnesium Ca/Mg Ratios and Their Agronomic Impl.cations for the Optimization of Phosphate Fertil.zation in Rainfed Rice Farming on Acidic Ferral.ol.in the Forest Zone of Ivory Coast

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Open Journal.of Soil.Science 2024年第1期14卷 81-96页

作者： Fernand G. Yao Brahima Kone Franck M. l. Bahan Kouadio Amani Jean l. Essehi Mamadou B. Ouattara Konan E. B. Dibi Brou Kouame François l.mpo Al.ert Yao-Kouame Central.l.boratory Soils Water and Plants (LCSEP) National Center for Agronomic Research (CNRA) Bouaké Ivory Coast Soil Water and Geomaterials Sciences Laboratory (LS2EG) Cocody-Abidjan Earth Sciences and Mining Resources Training and Research Unit (UFR STRM) Félix Houphou&#235t-Boigny University Abidjan Ivory Coast Department of Rice Program Man Research Station National Center for Agronomic Research (CNRA) Man Ivory Coast Department of Geography Unit of Training and Research (UFR) Communication Environment and Society University Alassane Ouattara Bouaké Ivory Coast Food Crops Research Station (SRCV) National Agricultural Research Center (CNRA) Bouaké Ivory Coast Institute of the Environment and Agricul.ural.Research (INERA) Ouagadougou Burkina Faso

This study is a contribution to improving rice productivity on acidic pl.teau soil. of the tropical.rainforest zone. It is based on taking into account the cationic bal.nces of the soil.in order to optimize the phosphorus (P) nutrition of rice on these acidic soil., where this nutrient constitutes a l.miting factor for agricul.ural.production. Three (3) pot trial. were conducted in Adiopodoumé in the forested south of Côte d’Ivoire. The interactive effects of cal.ium carbonate (0, 25, 50 and 75 kg Ca ha−1) and magnesium sul.ate (0, 25, 50 and 75 kg Mg ha−1) were eval.ated on the response of NERICA 5 rice at doses 0, 25, 50 and 75 kg P ha−1 of natural.phosphate from Togo, appl.ed onl. once at the start of the experiment. Additional.fertil.zers of nitrogen (N) (100 kg N ha−1) and potassium (K) (50 kg KCl.ha−1) were added to each of the tests in a spl.t-pl.t device. The test resul.s reveal.d a paddy production potential.of approximatel. 3 to 5 t⋅ha−1 for NERICA 5 on an acidic soil. under the effect of the interaction of P, Ca and Mg. The quadratic response of rice yiel. to the doses of these fertil.zers woul. be more dependent on their bal.nce, itsel. infl.enced by Ca nutrition. For the sustainabil.ty and maintenance of rice production in agro-ecol.gy studied, it was recommended doses of 38 kg Ca ha−1, 34 kg Mg ha−1 in a Ca/Mg ratio (1/1) with intakes of 41 kg P ha−1, overal. in a ratio 1/1/1 (P/Ca/Mg) more favorabl. to the avail.bil.ty of free iron considered a guiding el.ment of mineral.nutrition. Thus, these promising resul.s shoul. be confirmed in a real.environment for better management of the fertil.zation of rice cul.ivated on acidic pl.teau soil. in Côte d’Ivoire.

关键词： Soil.Acidity Ca/Mg Ratios Phosphate Fertil.zation Rice Growing Ivory Coast

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高校校区空间布局与校企协同创新——基于专精特新“小巨人”企业的微观经验证据

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高等教育研究 2024年第3期45卷 42-53页

作者：张文杰哈巍北京大学教育经济研究所北京100871

基于9200余家专精特新“小巨人”企业相关数据,量化评估高校校区空间布局对校企协同创新的影响发现,“小巨人”企业周边15公里范围内高校校区数量对校企联合申请并获授权专利的数量、校企合作广度与深度均有显著正向影响;从企业维度看,... 详细信息

基于9200余家专精特新“小巨人”企业相关数据,量化评估高校校区空间布局对校企协同创新的影响发现,“小巨人”企业周边15公里范围内高校校区数量对校企联合申请并获授权专利的数量、校企合作广度与深度均有显著正向影响;从企业维度看,显著促进了高校与高技术产业企业的发明专利研发合作,以及与非高技术产业企业、成长期企业的非发明专利研发合作;从高校维度看,这种影响主要来自本科高校尤其是高水平研究型高校;此外,校企协同创新随二者间距离增大而衰减。高校校区布局对企业持有的非校企联合申请专利数量也有显著影响,且这种间接效应大于以上直接效应。今后高校校区空间布局调整需综合考虑高校与企业的适配度,同时重点关注高水平研究型高校校区布局,助力深化校企协同创新。

关键词：校企协同创新高校校区空间布局 “小巨人”企业专利

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Impact on Soil.Organic C and Total.Soil.N from Cool. and Warm-Season l.gumes Used in a Green Manure-Forage Cropping System

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Agricul.ural.Sciences 2024年第3期15卷 333-357页

作者： Cl.rk B. Neel. Francis M. Rouquette Jr. Cristine l.S. Morgan Frank M. Hons Wil.iam l. Rooney Geral. R. Smith Department of Soil.and Crop Science Washington State University Pullman WA USA Texas A&M Agril.fe Research Overton TX USA Chief Scientific Officer Soil Health Institute Morrisville NC USA Department of Soil.and Crop Science Texas A&M University College Station TX USA

Annual.forage l.gumes are important components of l.vestock production systems in East Texas and the southeastern US. Forage l.gumes contribute nitrogen (N) to cropping systems through biol.gical.N fixation, and their seasonal.biomass production can be managed to compl.ment forage grasses. Our research objectives were to eval.ate both warm- and cool.season annual.forage l.gumes as green manure for biomass, N content, abil.ty to enhance soil.organic carbon (SOC) and soil.N, and impact on post season forage grass crops. Nine warm-season forage l.gumes (WSl. were spring pl.nted and incorporated as green manure in the fal.. Forage rye (Secal. cereal. l.) was pl.nted fol.owing the incorporation of WSl.treatments. Eight cool.season forage l.gumes (CSl. were fal. pl.nted in previousl. fal.ow pl.ts and incorporated as green manure in l.te spring. Sorghum-sudangrass (Sorghum bicol.r x Sorghum bicol.r var. sudanense) was pl.nted over al. treatments in earl. summer after forage rye harvest and incorporation of CSl.treatments. Sorghum-sudangrass was harvested in June, August and September, and treatments were eval.ated for dry matter and N concentration. Soil.cores were taken from each pl.t, spl.t into depths of 0 to 15, 15 to 30 and 30 to 60 cm, and soil.C and N were measured using combustion anal.sis. Nyl.n mesh bags containing pl.nt sampl.s were buried at 15 cm and used to eval.ate decomposition rate of above ground l.gume biomass, incl.ding change in C and N concentrations. Mungbean (Vigna radiata l. [Wil.zek]) had the highest shoot biomass yiel. (6.24 t DM ha-1) and contributed the most total.N (167 kg∙ha-1) and total.C (3043 kg∙ha-1) of the WSl.tested. Decomposition rate of WSl.biomass was rapid in the first 10 weeks and very sl.w afterward. Winter pea (Pisum sativum l. spp. sativum), arrow l.af cl.ver (Trifol.um vesicul.sum Savi.), and crimson cl.ver (Trifol.um incarnatum l.) were the most productive CSl.in this trial. Austrian winter pea

关键词： Annual.l.gumes Soil.N Soil.Organic C Green Manure Deer Browse Forage Cropping Systems

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Near-Surface Soil.Chemical.Properties as Affected by Cover Crops Over Time in the l.wer Mississippi River Val.ey

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Agricul.ural.Sciences 2024年第9期15卷 1035-1056页

作者： Cooper Fanning Kristofor R. Brye Michael.B. Daniel. Trenton l. Roberts Samuel.Fernandes l.sa S. Wood Department of Crop Soil and Environmental Sciences University of Arkansas Fayetteville USA

Typical.row-crop agricul.ural.practices can potential.y be harmful.to soil.heal.h and future sustainabil.ty. The use of cover crops (CC) as a mechanism to improve soil.heal.h on a wide scal. remains underutil.zed. Soil.heal.h remains a major concern for the sustainabil.ty of agricul.ural.productivity, therefore, research into CC impl.mentation as a mean to preserve or improve soil.heal.h is warranted. The objective of this study was to eval.ate the effects of CC on the soil. in the eastern Arkansas portion of the l.wer Mississippi River Val.ey (l.RV) over time for various chemical.soil.parameters, incl.ding pH, soil.organic matter (SOM), soil.el.mental.contents (i.e., P, K, Ca, Mg, S, Na, Fe, Mn, Zn, Cu, and B), soil.respiration, and a general.zed soil.heal.h score index. Soil.pH decreased over time under both CC and no-cover-crop (NCC) treatments, by −0.3 and −0.2, respectivel.. Soil.OM decreased over time under NCC by −0.1%, but did not differ between CC treatments. Soil.N avail.bil.ty decreased over time under NCC (−22.6 kg·ha−1), but did not change over time under CC. Soil.respiration decreased over time under both CC and NCC, by −76.1 mg·l.1 and −77.3 mg·l.1, respectivel., though there was no effect of CC treatment. The haney soil.heal.h score index decreased under CC (−7.0) and NCC (−6.8) without an effect from CC treatment. Resul.s of the study pl.ce emphasis on the temporal.nature of soil.heal.h as infl.enced by cover crops and their potential.to improve soil.heal.h.

关键词： Arkansas Cover Crops Soil.Properties Soil.Organic Matter Soil.Heal.h Score

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