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Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

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Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim Kyeong-Hyeon Eom Dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun Kim Jun-Koo Yi Jae-Jung ha Ji-Hyun Lee Seong-Beom Lee Woo-Jae Son Soo-Young Yum Won-Wu Lee Goo Jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing has been considered as powerful tool in agricultural ***,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising ***,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in *** this point of view,electroporation in embryos has been risen as an *** First,editing efficiency of our electroporation methods were tested for *** of mutation on embryo was confirmed by T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both *** These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively ***,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

An oxygenating colloidal bioink for the engineering of biomimetic tissue constructs

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Bio-Design and Manufacturing 2024年第3期7卷 240-261页

作者： Seol-ha Jeong Jarno Hiemstra Patrick V.Blokzijl Rebeca Damian-Ferrara Danilo Martins dos Santos Jéssica H.L.da Fonseca Min-Ho Kang Jihyun Kim Dilara Yilmaz-Aykut Mei L.L.Cham-Pérez Jeroen Leijten Su Ryon Shin Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMA 02139USA Leijten Lab Department of Developmental BioEngineeringFaculty of Science and TechnologyTechnical Medical CentreUniversity of TwenteDrienerlolaan 57522 NB EnschedeThe Netherlands Nanotechnology National Laboratory for Agriculture(LNNA) Embrapa InstrumentaçãoSao CarlosSao Paulo 13560-970Brazil Department of Biomedical-Chemical Engineering The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea Department of Biotechnology The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea

Ensuring a sufficient oxygen supply is pivotal for the success of bioprinting applications since it fosters tissue integration and natural *** in oxygen concentration among diverse tissues necessitates the precise recreation of tissue-specific oxygen levels in imprinted constructs to support the survival of targeted *** oxygen-releasing biomaterials,such as oxygen-generating microparticles(OMPs),have shown promise for enhancing the oxygen supply of microenvironments in injured tissues,whether this approach is scalable for large tissues and whether tissue-specific bioinks with varying OMP concentrations remain printable remain *** study addresses this critical gap by introducing an innovative class of engineered oxygenated bioinks that combine colloidal-based microgels with *** report that incorporating nanosized calcium peroxide(nCaO_(2))and manganese oxide nanosheets(nMnO_(2))into hydrophobic polymeric microparticles enables precise modulation of oxygen release while controlling hydrogen peroxide ***,the fabrication of oxygenating and cytocompatible colloidal gels is achieved using an aqueous two-phase *** study thoroughly evaluates the fundamental characteristics of the resulting bioink,including its rheological behaviors,printability,shape fidelity,mechanical properties,and oxygen release ***,this study demonstrates the macroscopic scalability and cytocompatibility of printed constructs produced via cell-laden oxygenating colloidal *** showcasing the effectiveness of extrusion-based bioprinting,this study underscores how it can be used to fabricate biomimetic tissues,indicating its potential for new *** findings presented here advance the bioprinting field by achieving scalability with both high cell viability and the possibility of mimicking specifically oxygenated *** work thereby offers a promising avenue for the development of functional tissues with e

关键词： 3D bioprinting Bioink Colloidal gels Extrusion printing Oxygen-generating microparticle

Predicting 3D radiotherapy Dose-Volume Based on Deep Learning

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Intelligent Automation & Soft Computing 2024年第2期39卷 319-335页

作者： Do Nang Toan Lam Thanh Hien ha Manh Toan Nguyen Trong Vinh Pham Trung Hieu Institute of Information Technology Vietnam Academy of Science and TechnologyHanoi10072Vietnam Faculty of Information Technology Lac Hong UniversityBien HoaDong Nai76120Vietnam

Cancer is one of the most dangerous diseaseswith *** of the principal treatments is radiotherapy by using radiation beams to destroy cancer cells and this workflow requires a lot of experience and skill from doctors and *** our study,we focused on the 3D dose prediction problem in radiotherapy by applying the deeplearning approach to computed tomography(CT)images of cancer *** image data has more complex characteristics than normal image data,and this research aims to explore the effectiveness of data preprocessing and augmentation in the context of the 3D dose prediction *** proposed four strategies to clarify our hypothesis in different aspects of applying data preprocessing and *** strategies,we trained our custom convolutional neural network model which has a structure inspired by the U-net,and residual blocks were also applied to the *** output of the network is added with a rectified linear unit(Re-Lu)function for each pixel to ensure there are no negative values,which are absurd with radiation *** experiments were conducted on the dataset of the Open Knowledge-Based Planning Challenge which was collected from head and neck cancer patients treatedwith radiation *** results of four strategies showthat our hypothesis is rational by evaluating metrics in terms of the Dose-score and the Dose-volume histogram score(DVH-score).In the best training cases,the Dose-score is 3.08 and the DVH-score is *** addition,we also conducted a comparison with the results of another study in the same context of using the loss function.

关键词： CT image 3D dose prediction data preprocessing augmentation

Endobronchial metastasis secondary to renal clear cell carcinoma:A case report

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World Journal of Clinical Cases 2024年第23期12卷 5416-5421页

作者： Tian-hao Xie Yan Fu Si-Ning ha Qing-Xu Meng Qian Sun Pan Wang Department of General Surgery The Affiliated Hospital of Hebei UniversityBaoding 071000Hebei ProvinceChina Department of Ophthalmology Baoding First Central HospitalBaoding 071000Hebei ProvinceChina Department of Pathology The Affiliated Hospital of Hebei UniversityBaoding 071000Hebei ProvinceChina

BACKGROUND Endobronchial metastases(EBMs)are tumours that metastasise from a malignant tumour outside the lungs to the central and subsegmental bronchi,and are visible under a *** EBMs are formed by direct invasion or metastasis of intrathoracic malignant tumours,such as lung cancer,oesophageal cancer or mediastinum *** cell carcinoma(RCC),accounting for 2%to 3%of all tumours,is a common malignant tumour of the urinary *** clear cell carcinoma(RCCC)constitutes the predominant pathological subtype of RCC,comprising approximately 70%to 80%of all RCC *** can spread and metastasise through arterial,venous and lymphatic circulation to almost all organs of the ***,lung,bone,liver,brain and local recurrence are the most common metastatic neoplasms of ***,EBM from RCCC has a low complication rate and is often misdiagnosed as primary lung *** SUMMARY A 71-year-old male patient who had undergone radical left nephrectomy 7 years prior due to RCCC was referred to our hospital due to a 1-mo history of productive *** results of an enhanced chest CT scan indicated the presence of a soft tissue nodule in the upper lobe of the left lung,and flexible bronchoscopy revealed a hypervascular lesion in the bronchus of the left lung's superior ***,the patient underwent thoracoscopic left superior lobe wedge resection,and pathology confirmed EBM from the *** EBM from RCCC has a low incidence and no characteristic clinical manifestations in the early *** a bronchial tumour is found in a patient with RCCC,the possibility of bronchial metastatic cancer should be considered.

关键词： Endobronchial metastases Clear renal cell carcinoma Renal cell carcinoma diagnosis Treatment Case report

Tuberculosis Diagnosis and Visualization with a Large Vietnamese X-ray Image Dataset

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Intelligent Automation & Soft Computing 2024年第2期39卷 281-299页

作者： Nguyen Trong Vinh Lam Thanh Hien ha Manh Toan Ngo Duc Vinh Do Nang Toan Faculty of Information Technology Lac Hong UniversityBien HoaDong Nai76120Vietnam Institute of Information Technology Vietnam Academy of Science and TechnologyHanoi10072Vietnam Faculty of Information Technology Hanoi University of IndustryHanoi11915Vietnam

Tuberculosis is a dangerous disease to human life,and we need a lot of attempts to stop and reverse ***,in theCOVID-19 pandemic,access to medical services for tuberculosis has become very *** late detection of tuberculosis could lead to danger to patient health,even *** one of the countries heavily affected by the COVID-19 pandemic,andmany residential areas as well as hospitals have to be isolated for a long *** demands a fast and effective tuberculosis diagnosis solution to deal with the difficulty of accessingmedical services,such as an automatic tuberculosis diagnosis *** our study,aiming to build that system,we were interested in the tuberculosis diagnosis problem from the chest X-ray images of Vietnamese *** chest X-ray image is an important data type to diagnose tuberculosis,and it has also received a lot of attention from deep learning *** paper proposed a novel method for tuberculosis diagnosis and visualization using the deeplearning approach with a large Vietnamese X-ray image *** detail,we designed our custom convolutional neural network for the X-ray image classification task and then analyzed the predicted result to provide visualization as a *** prove the performance of our network model,we conducted several experiments to compare it to another study and also to evaluate it with the dataset of this *** support the implementation,we built a specific annotation system for tuberculosis under the requirements of radiologists in the Vietnam National Lung Hospital.A large experiment dataset was also from this hospital,and most of this data was for training the convolutional neural network *** experiment results were evaluated regarding sensitivity,specificity,and *** achieved high scores with a training accuracy score of 0.99,and the testing specificity and sensitivity scores were over *** on the X-ray image classification result,we visualize pr

关键词： Tuberculosis classification Vietnamese chest X-ray deep learning

“腾笼换鸟”:高校布局调整与区县产业协调发展

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北京大学教育评论 2024年第1期22卷 41-59,189页

作者：林璐朱琼哈巍北京大学教育经济研究所北京100871

本文借助中国高等教育扩招浪潮下新建高校或校区的准实验,考察高校区域布局调整对区县产业协调发展的影响。本文发现,新建高校或校区对所在区县的新企业数量带来了消极影响,但对新企业的产业结构高级化水平起到了显著的积极影响,由此发... 详细信息

本文借助中国高等教育扩招浪潮下新建高校或校区的准实验,考察高校区域布局调整对区县产业协调发展的影响。本文发现,新建高校或校区对所在区县的新企业数量带来了消极影响,但对新企业的产业结构高级化水平起到了显著的积极影响,由此发挥出促进地方产业“腾笼换鸟”的功能。在区域协调发展方面,新建高校或校区在距离省会城市中心较远的地区同样发挥出了促进当地新企业产业结构高级化的功能。从全国跨区域比较来看,新建高校或校区具有“放大”区位劣势的效应,非东部地区的院校或校区对当地新企业产业结构高级化的促进作用较弱。应积极发挥高校通过影响产业布局促进地方产业结构转型升级的作用,实现地方高校特色办学与产业特色发展相结合,推动区域高等教育与经济建设协调发展。

关键词：产业布局高等教育准实验产业结构升级区域协调发展

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2018—2022年广东省5岁以下儿童意外死亡流行分析

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中国儿童保健杂志 2024年第2期32卷 218-222页

作者：哈林娜徐昊立暨南大学基础医学与公共卫生学院广东广州510632 广东省妇幼保健院保健部广东广州510632

目的了解2018—2022年广东省5岁以下儿童意外死亡的主要死因及其流行病学分布情况,为制订进一步的预防措施和干预方法提供理论依据。方法以广东省2018—2022年死亡的5岁以下儿童为研究对象,将ICD10中S00-T98和V01-Y98系列疾病作为分... 详细信息

目的了解2018—2022年广东省5岁以下儿童意外死亡的主要死因及其流行病学分布情况,为制订进一步的预防措施和干预方法提供理论依据。方法以广东省2018—2022年死亡的5岁以下儿童为研究对象,将ICD10中S00-T98和V01-Y98系列疾病作为分类标准筛选出其中意外原因导致死亡的个案,计算相关的率、构成比等,分析儿童意外死亡原因及三间分布。结果 2018—2022年广东省总死亡率为2.94‰,死亡人数21 329人,其中意外死亡人数3 819人(0.53‰),男女性别比为1.29∶1,不同年龄组间意外伤害的性别构成差异有统计学意义(χ^(2)=16.38,P<0.01)。意外死亡原因前三位为意外窒息(1 590/3 819)、溺水(926/3 819)和交通意外(527/3 819)。各年龄段男童意外死亡率均高于女童,差异无统计学意义(χ^(2)=13.68,P=0.19),不同意外死因儿童的年龄组差异有统计学意义(χ^(2)=1 723.28,P<0.01),死前救治情况差异有统计学意义(χ^(2)=174.31,P<0.01)。结论广东省5岁以下儿童死亡的主要原因为意外窒息、溺水。应分年龄采取不同的预防措施提高儿童及家长的意外伤害识别防范意识,降低5岁以下儿童意外死亡率。

关键词： 5岁以下儿童意外死亡意外伤害

High Na-ion conductivity andmechanical integrity of anion-exchanged polymeric hydrogel electrolytes for flexible sodium ion hybrid energy storage

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SusMat 2024年第1期4卷 140-153页

作者： JungWoo Hong harpalsinh H.rana Jeong Hee Park Jun Su Kim Sang Joon Lee Gun Jang Tae Hoon Kang Kang Ho Shin Sang ha Baek Wooseok Yang Kwang Ho Kim Ju-Hyuk Lee Ho Seok Park School of Chemical Engineering Sungkyunkwan UniversitySuwonRepublic of Korea Clean Energy Research Center Korea Institute of Science and Technology(KIST)SeoulRepublic of Korea KIST-SKKU Carbon-Neutral Research Center Sungkyunkwan University(SKKU)SuwonRepublic of Korea SKKU Institute of Energy Science and Technology(SIEST) Sungkyunkwan UniversitySuwonRepublic of Korea COSMOS LAB/92 Jomaru-ro 385beon-gilBucheon-siRepublic of Korea SKKU Advanced Institute of Nano Technology(SAINT) Sungkyunkwan UniversitySuwonRepublic of Korea Department of Health Sciences and Technology Samsung Advanced Institute for Health Sciences and Technology(SAIHST)Sungkyunkwan UniversitySuwonRepublic of Korea

The polymeric gel electrolytes are attractive owing to their higher ionic conductivities than those of dry polymer electrolytes and lowered water activity for enlarged potential ***,the ionic conductivity and mechanical strength of the Na-ion conducting polymeric gel electrolytes are limited by below 20 mS cm−1 and 2.2 ***,we demonstrate Na-ion conducting and flexible polymeric hydrogel electrolytes of the chemically coupled poly(diallyldimethylammonium chloride)-dextrin-N,N′-methylene-bisacrylamide film immersed in NaClO_(4) solution(ex-DDA-Dex+NaClO_(4))for flexible sodium-ion hybrid capacitors(f-NIHC).In particular,the anion exchange reaction and synergistic interaction of ex-DDA-Dex with the optimum ClO_(4)−enable to greatly improve the ionic conductivity up to 27.63 mS cm−1 at 25◦C and electrochemical stability window up to 2.6 V,whereas the double networking structure leads to achieve both the mechanical strength(7.48 MPa)and softness of hydrogel ***,the f-NIHCs with the ex-DDA-Dex+NaClO_(4) achieved high specific and high-rate capacities of 192.04 F g^(−1)at 500 mA g^(−1)and 116.06 F g^(−1)at 10000 mA g^(−1),respectively,delivering a large energy density of 120.03Wh kg^(−1)at 906Wkg^(−1)and long cyclability of 70%over 500 cycles as well as demonstrating functional operation under mechanical stresses.

关键词： anion exchange chemistry flexible energy storage hybrid energy storage hydrogel electrolytes renewable electrolytes sodium storage

高校校区空间布局与校企协同创新——基于专精特新“小巨人”企业的微观经验证据

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高等教育研究 2024年第3期45卷 42-53页

作者：张文杰哈巍北京大学教育经济研究所北京100871

基于9200余家专精特新“小巨人”企业相关数据,量化评估高校校区空间布局对校企协同创新的影响发现,“小巨人”企业周边15公里范围内高校校区数量对校企联合申请并获授权专利的数量、校企合作广度与深度均有显著正向影响;从企业维度看,... 详细信息

基于9200余家专精特新“小巨人”企业相关数据,量化评估高校校区空间布局对校企协同创新的影响发现,“小巨人”企业周边15公里范围内高校校区数量对校企联合申请并获授权专利的数量、校企合作广度与深度均有显著正向影响;从企业维度看,显著促进了高校与高技术产业企业的发明专利研发合作,以及与非高技术产业企业、成长期企业的非发明专利研发合作;从高校维度看,这种影响主要来自本科高校尤其是高水平研究型高校;此外,校企协同创新随二者间距离增大而衰减。高校校区布局对企业持有的非校企联合申请专利数量也有显著影响,且这种间接效应大于以上直接效应。今后高校校区空间布局调整需综合考虑高校与企业的适配度,同时重点关注高水平研究型高校校区布局,助力深化校企协同创新。

关键词：校企协同创新高校校区空间布局 “小巨人”企业专利