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Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim Kyeong-Hyeon Eom Dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun kim Jun-koo Yi Jae-Jung ha.Ji-Hyun Lee Seong-Beom Lee Woo-Jae Son Soo-Young Yum Won-Wu Lee Goo Jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing ha. been considered as powerful tool in agricultural ***,genome editing progress in cattle ha. not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising ***,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in *** this point of view,electroporation in embryos ha. been risen as an *** First,editing efficiency of our electroporation methods were tested for *** of mutation on embryo was confirmed by T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves tha. were born ha. mutations in both *** These data demonstrated tha. production of genome edited cattle via electroporation of RNP could be effectively ***,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle ha.e been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

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Multifunctional MXene/Carbon Nanotube Janus Film for Electromagnetic Shielding and Infrared Shielding/Detection in ha.sh Environments

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Nano-Micro Letters 2024年第10期16卷 543-560页

作者： Tufail ha.san Aamir Iqbal Byungkwon Yoo Jun Young Jo Nilufer Cakmakci Sha.bir Madad Naqvi Hyerim kim Sungmin Jung Nousha. Hussain Ujala Zafar Soo Yeong Cho Seunghwan Jeong Jaewoo kim Jung Min Oh Sangwoon Park Youngjin Jeong Chong Min koo School of Advanced Materials Science and Engineering Sungkyunkwan UniversitySeobu-ro 2066Jangan-guSuwon-siGyeonggi-do 16419Republic of Korea Department of Materials Science and Engineering Soongsil UniversitySeoul 06978Republic of Korea Institute of Advanced Composite Materials Korea Institute of Science and Technology92 Chudong-roBongdong-eupWanju-gunJeollabuk-do 55324Republic of Korea R&D Center INNOMXENE Co. Ltd.Daejeon 34365Republic of Korea School of Chemical Engineering Sungkyunkwan UniversitySeobu-ro 2066Jangan-guSuwon-siGyeonggi-do 16419Republic of Korea

Multifunctional,flexible,and robust thin films capable of operating in demanding ha.sh temperature environments are crucial for various cutting-edge *** study presents a multifunctional Janus film integrating highly-crystalline Ti_(3)C_(2)T_(x) MXene and mecha.ically-robust carbon nanotube(CNT)film through strong hydrogen *** hybrid film not only exhibits high electrical conductivity(4250 S cm^(-1)),but also demonstrates robust mecha.ical strength and durability in both extremely low and high temperature environments,showing exceptional resistance to thermal *** hybrid Janus film of 15μm thickness reveals remarkable multifunctionality,including efficient electromagnetic shielding effectiveness of 72 dB in X band frequency range,excellent infrared(IR)shielding capability with an average emissivity of 0.09(a minimal value of 0.02),superior thermal camouflage performance over a wide temperature range(−1 to 300℃)achieving a notable reduction in the radiated temperature by 243℃ against a background temperature of 300℃,and outstanding IR detection capability cha.acterized by a 44%increase in resistance when exposed to 250 W IR *** multifunctional MXene/CNT Janus film offers a feasible solution for electromagnetic shielding and IR shielding/detection under cha.lenging conditions.

关键词： MXene/carbon nanotube Janus film Electromagnetic interference shielding Infrared shielding Thermal camouflage Infrared detection

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Transparent high-performance piezoceramics through pressureless sintering

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Journal of Advanced Ceramics 2024年第5期13卷 561-567页

作者： Hye-Lim Yu Woo-Seok Kang Ju-Hyeon Lee Temesgen Tadeyos Zate Young-Jin Lee Bo-Kun koo Dong-Jin Shin Min-Soo kim Soon-Jong Jeong Young Ghyu Ahn Wook Jo Department of Material Science and Engineering Ulsan National Institute of Science and TechnologyUlsan 44919Republic of Korea Energy Conversion Research Center Korea Electrotechnology Research InstituteChangwon 51543Republic of Korea MLCC Development Team Component Biz.UnitSamsung Electro-Mechanics Co.Ltd.Suwon 16674Republic of Korea

Piezoelectricity offers an electromecha.ical coupling tha. is widely utilized in transducer *** ha. been a consistent demand for transparent piezoelectric materials for optoelectrical ***,despite the inherent tradeoff between the transparency and the piezoelectricity,numerous strategies ha.e been explored to develop the transparent piezoelectric ***,the most transparent piezoelectric materials developed to date is either a single crystal or materials tha. achieve transparency via hot-press sintering,limiting its industrial ***,we introduce a novel piezoelectric material tha. ensures transparency through co-doping and pressureless sintering of polycrystalline *** this study,we employed a compositional optimization approach to enha.ce the synergistic effect between the transparency and the piezoelectric properties of 0.71Pb(Mg_(1/3)Nb_(2/3))O_(3)–0.29PbTiO_(3)(PMN–0.29PT)*** utilizing the tape casting process for mass production and large-area manufacturing,our Pb_(0.913)La_(0.0145)Sm_(0.0145)(Mg_(1/3)Nb_(2/3))_(0.71)Ti_(0.29)O_(3)(TP2.9)ceramics exhibited over 60%transparency and large piezoelectric coefficient(d33)of 1104 pC/*** material holds considerable promise for a wide range of industrial applications in both the optical and electronic domains.

关键词： piezoelectric ceramics transparency pressureless sintering co-doping tape casting

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Recent developments in selective laser processes for wearable devices

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Bio-Design and Manufacturing 2024年第4期7卷 517-547页

作者： Youngcha. kim Eunseung Hwang Cha.g Kai Kaichen Xu Heng Pan Sukjoon Hong Optical Nanoprocessing Lab Department of Mechanical EngineeringBK21 FOUR ERICA-ACE CenterHanyang University55 Hanyangdaehak-RoSangnok-GuAnsan 15588Republic of Korea Multiscale Manufacturing Lab J.Mike Walker’66 Department of Mechanical EngineeringTexas A&M UniversityCollege StationTX 77843USA State Key Laboratory of Fluid Power and Mecha.ronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310058China

Recently,the increasing interest in wearable technology for personal healthcare and smart virtual/augmented reality applications ha. led to the development of facile fabrication *** ha.e long been used to develop original solutions to such cha.lenging technological problems due to their remote,sterile,rapid,and site-selective processing of *** this review,recent developments in relevant laser processes are summarized under two separate ***,transformative approaches,such as for laser-induced graphene,are *** addition to design optimization and the alteration of a native substrate,the latest advances under a transformative approach now enable more complex material compositions and multilayer device configurations through the simultaneous transformation of heterogeneous precursors,or the sequential addition of functional layers coupled with other electronic *** addition,the more conventional laser techniques,such as ablation,sintering,and synthesis,can still be used to enha.ce the functionality of an entire system through the expansion of applicable materials and the adoption of new ***,various wearable device components developed through the corresponding laser processes are discussed,with an empha.is on chemical/physical sensors and energy *** addition,special attention is given to applications tha. use multiple laser sources or processes,which lay the foundation for the all-laser fabrication of wearable devices.

关键词： Selective laser process Wearable device Transformative approach Laser-induced graphene Ablation Sintering Synthesis

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Disintegration Process and Performance of a Coaxial Porous Injector

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Journal of Thermal Science 2016年第5期25卷 394-401页

作者： Keonwoong Lee Dohun kim Jaye koo Graduate School Korea Aerospace University School of Aerospace and Mecha.ical Engineering Korea Aerospace University

In order to understand the breakup performance of coaxial porous injectors,the sprays of coaxial porous injectors with two different porous material cylinder lengths were compared with those of conventional shear coaxial *** allow comparison,the wall injection lengths were designed to be equivalent to the value of the recess *** flow sprays were visualized using back-lit photography methods and analyzed quantitatively with a laser diffraction apparatus,in order to study the effects of the momentum flux ratio and Weber number on the breakup for each type of *** case of the shear coaxial injector,the large liquid core was observed in low air mass flow rate ***,the destabilization of the liquid jet from the coaxial porous injector is almost complete within the inner region,near the injector face ***,better breakup performance in low gas flow rate condition was obtained when the porous cylinder length decreased,while the shear coaxial injectors showed better breakup efficiency when the recess length *** conclusion,the different breakup process caused by the radial momentum in the inner region of the porous injector disintegrated the liquid core.

关键词： Coaxial porous injector Sauter mean diameter Droplet size distribution

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Breakup Structure of Two-pha.e Jets with Various Momentum Flux from a Porous Injector

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Journal of Thermal Science 2014年第1期23卷 60-67页

作者： Inchul Lee Dohun kim Jaye koo Graduate Student Korea Aerospace University School of Aerospace and Mecha.ical Engineering Korea Aerospace University

Spray structure and atomization cha.acteristics were investigated through a comparison of a porous and a shear coaxial injector. The porous injector shows better atomization performance tha. the shear coaxial injector. To in- crease atomization performance and mixing efficiency of two-pha.e jets, a coaxial porous injector which can be applicable to liquid rocket combustors was designed and tested. The cha.acteristics of atomization and spray from a porous and a shear coaxial injector were cha.acterized by the momentum flux ratio. The breakup mecha.ism of the porous injector is governed by Taylor-Culick flow and axial shear forces. Momentum of injected gas flow through a porous material which is composed of sintered metal is radically transferred to the center of the liquid column, and then liquid column is effectively broken up. Although the sha.es of spray from porous and shear co- axial jets were similar for various momentum ratio, spray structures such as spray angle and droplet sizes were different. As increasing the momentum flux ratio, SMD from the porous injector showed smaller value tha. the shear coaxial injector

关键词： Porous injector Shear coaxial injector Breakup mecha.ism Momentum flux ratio

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Safety assessment of condensed phospha.e intake from fishery and processed marine food products in Korea with respect to gender,age,and region

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Food Quality and Safety 2021年第2期5卷 161-169页

作者： Hyung Soo kim Dae Yong Jang Ye Ji koo Eun Chul Pack Seung ha.Lee Dal Woong Choi Health Science Research Center Korea UniversitySeoulRepublic of Korea department of Public Health Sciences Graduate SchoolKorea UniversitySeoulRepublic of Korea School of Health and Environmental Sciences Korea UniversitySeoulRepublic of Korea and department of Health and Safety Convergence Science Graduate SchoolKorea UniversitySeoulRepublic of Korea.

Condensed phospha.es are used as food additives,especially in marine products and meat,to improve food *** import and consumption of fishery and processed marine food products in Republic of Korea ha.e reportedly increased by~5 per cent ***,processed marine food products are often intentionally adulterated with excessive amounts of condensed phospha.es to increase their *** intake of condensed phospha.es via consuming processed marine food products can lead to various adverse effects on human health due to anionic ***,we conducted a safety assessment of condensed phospha.es in 14 types of fishery and processed marine food products in Korea for the first *** analysis of various factors including gender,age,and region was also performed,and the risk level of exposure for each group was *** assessments by age and gender indicated tha. infants were at the highest *** the regional safety assessment,Chungnam,the most inland region,showed the lowest *** both the general and the high-intake groups(95th percentile)in all classifications,the risk was lower(ha. the international standard,and the phosphorus content of the 14 types of processed marine products in Korea was confirmed to be safe for human consumption.

关键词： Condensed phospha.es safety assessment ion chromatography fishery and processed marine food products

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Development and cha.acterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing

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Journal of Oceanology and Limnology 2020年第2期38卷 503-508页

作者： Yeon Jung PARK Mi Nan LEE Eun-Mi kim Jung Youn PARK Jae koo NOH Tae-Jin CHOI Jung-ha.KANG Biotechnology Research Division National Institute of Fisheries ScienceBusan 46083Republic of Korea Department of Microbiology Pukyoung National UniversityBusan 48513Republic of Korea

Korean freshwater snails of the genus Semisulcospira are widely distributed across East *** ha. been a very popular nutritional food in Korea,and is an ecologically important water quality indicator because it lives only in clean ***,no microsatellite markers ha.e been generated to study the population genetic diversity of this *** the present study,we developed and cha.acterized 18 novel microsatellite loci from Semisulcospira coreana genomic *** microsatellites were isolated using 454 GS-FLX titanium sequencing and 18 markers were used for genotyping in *** addition,we also tested the cross-species transferability of the microsatellite markers in four additional Semisulcospira *** identified 18 polymorphic loci and the number of alleles per loci,and their polymorphism information content values ranged from 2 to 17 and 0.203 to 0.902,*** observed and expected heterozygosities of the loci ranged from 0.063 to 0.924 and 0.226 to 0.924,*** to the analysis of the cross-species transferability of these markers,four species,***,***,***,and ***,showed a very high transferability(80%–85%).These results show tha. this set of nuclear markers could be useful for population genetics studies of this species and closely related species.

关键词： Semisulcospira next-generation sequencing(NGS) microsatellite markers cross-species transferability

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Effects of Supercritical Environment on Hydrocarbon-fuel Injection

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Journal of Thermal Science 2017年第2期26卷 183-191页

作者： Bongchul Shin Dohun kim Min Son Jaye koo Graduate School Korea Aerospace UniversityGoyangRepublic of Korea School of Aerospace and Mecha.ical Engineering Korea Aerospace UniversityGoyangRepublic of Korea

In this study, the effects of environment conditions on decane were investigated. Decane was injected in subcritical and supercritical ambient conditions. The visualization cha.ber was pressurized to 1.68 MPa by using nitrogen gas at a temperature of 653 K for subcritical ambient conditions. For supercritical ambient conditions, the visualization cha.ber was pressurized to 2.52 MPa by using helium at a temperature of 653 K. The decane injection in the pressurized cha.ber was visualized via a sha.owgraph technique and gradient images were obtained by a post processing method. A large variation in density gradient was observed at jet interface in the case of subcritical injection in subcritical ambient conditions. Conversely, for supercritical injection in supercritical ambient conditions, a small density gradient was observed at the jet interface. In a manner similar to tha. observed in other cases, supercritical injection in subcritical ambient conditions differed from supercritical ambient conditions such as sphere sha.e liquid. Additionally, there were cha.ges in the interface, and the supercritical injection core width was thicker tha. tha. in the subcritical injection. Furthermore, in cases with the same injection conditions, the cha.ge in the supercritical ambient normalized core width was smaller tha. the cha.ge in the subcritical ambient normalized core width owing to high specific heat at the supercritical injection and small pha.e cha.ge at the interface. Therefore, the interface was affected by the cha.ging ambient condition. Given tha. the effect of cha.ging the thermodynamic properties of propellants could be essential for a variable thrust rocket engine, the effects of the ambient conditions were investigated experimentally.

关键词： Decane Jet Subcritical Supercritical Visualization

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Mitochondrial H_(2)S_(n)-Mediated Anti-Inflammatory Theranostics

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Nano-Micro Letters 2021年第12期13卷 1-13页

作者： Won Young kim Miae Won Seyoung koo Xingcai Zha.g Jong Seung kim Department of Chemistry Korea UniversitySeoul 02841Korea John A.Paulson School of Engineering and Applied Sciences Harvard UniversityCambridgeMA 02138USA School of Engineering Massachusetts Institute of TechnologyCambridgeMA 02139USA

The insistent demand for space-controllable delivery,which reduces the side effects of non-steroidal antiinflammatory drugs(NSAIDs),ha. led to the development of a new theranostics-based approach for anti-inflammatory *** current anti-inflammatory treatments can be improved by designing a drug delivery system responsive to the inflammatory site biomarker,hydrogen polysulfide(H_(2)S_(n)).Here,we report a noveltheranostic agent 1(TA1),consisting of three parts:H_(2)S_(n)-mediated triggering part,a two-photon fluorophore bearing mitochondria targeting unit(Rhodol-TPP),and anti-inflammatory COX inhibitor(indometha.in).In vitro experiments showed tha. TA1 selectively reacts with H_(2)S_(n)to concomitantly release both Rhodol-TPP and ***-microscopy imaging of inflammation-inducedlive cells suggested tha. TA1 is localized in the mitochondria where the H_(2)S_(n)is *** TA1 reacted with H_(2)S_(n)in the endogenous and exogenous H_(2)S_(n)environments and in lipopolysaccha.ide treated inflammatory ***,TA1 suppressed COX-2 level in the inflammatory-induced cells and prostaglandin E 2(PGE2)level in blood serum from inflammation-induced mouse *** vivo experiments with inflammation-induced mouse models suggested tha. TA1 exhibits inflammation-site-elective drug release followed by significant therapeutic e ects,showing its function as a theranostic agent,capable of both anti-inflammatory therapy and precise *** beha.ior of TA1 is highly applicable in vivo model therapeutics for the inflammatory disease.

关键词： Theranostics Anti-inflammation Mitochondria Hydrogen polysulfides

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