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Ecological functions of uncultured microorganisms in the cobaltrich ferromanganese crust of a seamount in the central Pacific are elucidated by fosmid sequencing

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Acta Oceanologica Sinica 2015年第4期34卷 92-113页

作者： huo yingyi CHENG Hong Anton F.Post WANG Chunsheng JIANG Xiawei PAN Jie WU Min XU Xuewei Laboratory of Marine Ecosystem and Biogeochemistry Second Institute of Oceanography State Oceanic Administration State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of Oceanography College of Life Sciences Zhejiang University The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution Marine Biology Laboratory State Key Laboratory for Diagnosis and Treatment of Infectious Diseases the First Affiliated Hospital School of Medicine Zhejiang University

Cobalt-rich ferromanganese is an important seafloor mineral and is abundantly present in the seamount crusts. Such crusts form potential hotspots for biogeochemical activity and microbial diversity, yet our understanding of their microbial communities is lacking. In this study, a cultivation-independent approach was used to recover genomic information and derive ecological functions of the microbes in a sediment sample collected from the cobalt-rich ferromanganese crust of a seamount region in the central Pacific. A total of 78 distinct clones were obtained by fosmid library screening with a 16S rRNA based PCR method. Proteobacteria and MGI Thaumarch-aeota dominated the bacterial and archaeal 16S rRNA gene sequence results in the microbial community. Nine fosmid clones were sequenced and annotated. Numerous genes encoding proteins involved in metabolic functions and heavy metal resistance were identified, suggesting alternative metabolic pathways and stress responses that are essential for microbial survival in the cobalt-rich ferromanganese crust. In addition, genes that participate in the synthesis of organic acids and exoploymers were discovered. Reconstruction of the metabolic pathways revealed that the nitrogen cycle is an important biogeochemical process in the cobalt-rich ferromanganese crust. In addition, horizontal gene transfer （HGT） events have been observed, and most of them came from bacteria, with some occurring in archaea and plants. Clone W4-93a, belonging to MGI Thaumarch-aeota, contained a region of gene synteny. Comparative analyses suggested that a high frequency of HGT events as well as genomic divergence play important roles in the microbial adaption to the deep-sea environment.

关键词： seamount cobalt-rich ferromanganese crust metagenome horizontal gene transfer

Expression and Characterization of an NADP-Dependent Isocitrate Dehydrogenase from Halophilic Archaea Haloarcula marismortui

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Expression and Characterization of an NADP-Dependent Isocitr...

Cloning,expression and characterization of a salt-tolerant esterase from a marine bacterium Pelagibacterium halotolerans B2

第十届全国酶学学术讨论会

作者： Xiawei Jiang yingyi huo Xinqi Zhang Xufen Zhu 吴敏 College of Life Sciences Zhejiang University

Isocitrate dehydrogenase(ICDH) from the extremely halophilic archaea Haloarcula marismortui(HmICDH) is one of the key enzymes in tricarboxylic acid *** alignment showed HmICDH was similar to a hyperthermophilic ICDH from Archaeoglobus fulgidus(AfICDH) and secondary structure analysis revealed helix and strand regions were conserved in HmICDH compared with ***,HmICDH harbors more acidic residues(21.2%) than AfICDH(13.8%).In our study,we cloned the gene encoding for HmICDH

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Cloning,expression and characterization of a salt-tolerant e...

Research on performance optimization of virtual data space across WAN

第十届全国酶学学术讨论会

作者： Xiawei Jiang yingyi huo Xinqi Zhang Xufen Zhu 吴敏 College of Life Sciences Zhejiang University

An esterase PE10(279 aa) was cloned from Pelagibacterium halotolerans B2,which was a novel member of the family Hyphomicrobiaceae,isolated from East China ***10 was overexpressed in Escherichia coli Rosetta in a soluble form and was purified by Ni-NTA affinity chromatography *** deduced protein was 29.91 kDa and the phylogenetic analysis of the amino acids showed it represented a new family of lipolytic *** substrate specificity study showed PE10 preferred short chain p-nitrophenyl esters and exhibited

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Frontiers of Computer Science 2024年第6期18卷 186505-186505页

作者： huo Jiantong huo Zhisheng XIAO Limin HE Zhenxue State Key Laboratory of Software Development Environment Beihang University School of Computer Science and Engineering Beihang University High Performance Computing Center Beihang University Hebei Key Laboratory of Agricultural Big Data Hebei Agricultural University

For the high-performance computing in a WAN environment, the geographical locations of national supercomputing centers are scattered and the network topology is complex, so it is difficult to form a unified view of resources. To aggregate the widely dispersed storage resources of national supercomputing centers in China, we have previously proposed a global virtual data space named GVDS in the project of “High Performance Computing Virtual Data Space”, a part of the National Key Research and Development Program of China. The GVDS enables large-scale applications of the high-performance computing to run efficiently across WAN. However, the applications running on the GVDS are often data-intensive, requiring large amounts of data from multiple supercomputing centers across WANs. In this regard, the GVDS suffers from performance bottlenecks in data migration and access across WANs. To solve the above-mentioned problem, this paper proposes a performance optimization framework of GVDS including the multitask-oriented data migration method and the request access-aware IO proxy resource allocation strategy. In a WAN environment, the framework proposed in this paper can make an efficient migration decision based on the amount of migrated data and the number of multiple data sources, guaranteeing lower average migration latency when multiple data migration tasks are running in parallel. In addition, it can ensure that the thread resource of the IO proxy node is fairly allocated among different types of requests (the IO proxy is a module of GVDS), so as to improve the application’s performance across WANs. The experimental results show that the framework can effectively reduce the average data access delay of GVDS while improving the performance of the application greatly.

关键词： storage aggregation across WANs large-scale applications GVDS data migration allocation of IO proxy resource

Motion-pressure coupled control and simulation of long-endurance capability for multicapsule stratospheric airships

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Chinese Journal of Aeronautics 2024年第6期37卷 137-150页

作者： Zhiguang SHI Wei huo Zongyu ZUO School of Automation Science and Electrical Engineering Beihang UniversityBeijing 100191China Beijing Near Space Airship Technology Development Co. LtdBeijing 100070China

The current study focuses on the motion-pressure coupled control for a multicapsule stratospheric airship and transforms the path-tracking and heading-hold control of airships into guidance tracking with a time-varying weighted sum of longitudinal and lateral velocities by the definition of compound ***,an improved nonlinear predictive control method is provided to reduce the control energy consumption by the rolling optimization of controller parameters based on finite time intervals,ensuring infinite-time path-tracking ***,combined with the proposed cyclic regulation process of safe pressure between internal and external capsules,this study can fully reflect the force-thermal coupled rule of airships under the actions of atmospheric environment and maneuvering force,while evaluating the long-endurance capability of airships under the conditions of safe superheating and *** effectiveness of the motionpressure coupled controller was verified through numerical simulations,which can overcome the influence of environmental wind and achieve a tracking effect for the desired cruise path and compound *** airspeed provided during the cyclic circadian time caused the maximum superheating of the helium controlled within 30*** helium in the internal and external capsules achieved circadian *** equivalent micropore diameter of the capsule of 5 mm can achieve 55 days of long-endurance *** controller satisfies the requirements of cruise-flight application modes for multicapsule stratospheric airships with important engineering value.

关键词： Compound speed Nonlinear predictive control Cyclic pressure regulation Long-endurance capability Stratospheric airship

全国大学生生命科学竞赛助力高校生命科学人才培养

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生物工程学报 2022年第5期38卷 2026-2032页

作者：霍颖异陈璨龚莺吴敏浙江大学国家级生物实验教学示范中心浙江杭州310058 浙江大学生命科学学院浙江杭州310058 浙江省微生物学会浙江杭州310058

全国大学生生命科学竞赛历经5届赛事,已成为国内最具规模和影响力的生命科学领域赛事。竞赛坚持“兴趣驱动、科学探究、过程评价、能力提升”的方针,竞赛的举办有效提升了高校生命科学类专业人才培养质量。本文介绍了竞赛的发展、组织... 详细信息

全国大学生生命科学竞赛历经5届赛事,已成为国内最具规模和影响力的生命科学领域赛事。竞赛坚持“兴趣驱动、科学探究、过程评价、能力提升”的方针,竞赛的举办有效提升了高校生命科学类专业人才培养质量。本文介绍了竞赛的发展、组织和管理,总结了竞赛的组织特色,可为各高校竞赛组织和学生参赛提供指导,助力全国高校生命科学领域的人才培养。

关键词：生命科学竞赛大学生科学探究组织特色

High performance photodegradation resistant PVA@TiO_(2)/carboxyl-PES self-healing reactive ultrafiltration membrane

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Chinese Journal of Chemical Engineering 2024年第2期66卷 31-39页

作者： Yu Liang Yuanfang Fan Zhongmin Su Mingxin huo Xia Yang Hongliang huo Chi Wang Zhi Geng College of Environment Engineering Research Center of Low-Carbon Treatment and Green Development of Polluted Water in Northeast ChinaMinistry of EducationNortheast Normal UniversityChangchun 130117China School of Science Hainan UniversityHaikou 570225China

The occurrence of ultrafiltration(UF)membrane fouling frequently hampers the sustainable advancement of UF *** self-cleaning UF membranes can effectively alleviate the problem of membrane ***,the self-cleaning process may accelerate membrane *** these concerns,we present an innovative design concept for composite self-healing materials based on self-cleaning UF *** begin,TiO_(2)nanoparticles were incorporated into the polymer molecular structure via molecular design,resulting in the synthesis of TiO_(2)/carboxyl-polyether sulfone(PES)hybrid ***,the nonsolvent-induced phase inversion technique was employed to prepare a novel of UF ***,a polyvinyl alcohol(PVA)hydrogel coating was applied to the hybrid UF membrane surface to create PVA@TiO_(2)/carboxyl-PES self-healing reactive UF *** establishing a covalent bond,the TiO_(2)nanoparticles were effectively and uniformly dispersed within the UF membrane,leading to exceptional self-cleaning ***,the water-absorbing and swelling properties of PVA hydrogel,along with its capacity to form hydrogen bonds with water molecules,resulted in UF membranes with improved hydrophilicity and active self-healing *** results demonstrated that the water contact angle of PVA@5%TiO_(2)/carboxyl-PES UF membrane was 43.1°.Following a 1-h exposure to simulated solar exposure,the water flux recovery ratio increased from 48.16%to 81.03%.Moreover,even after undergoing five cycles of 12-h simulated sunlight exposure,the UF membranes exhibited a consistent retention rate of over 97%,thus fully demonstrating their exceptional self-cleaning,antifouling,and selfhealing *** anticipate that the self-healing reactive UF membrane system will serve as a pioneering and comprehensive solution for the self-cleaning antifouling challenges encountered in UF membranes while also effectively mitigating the aging effects of reactive

关键词： Ultrafiltration membrane Self-cleaning Self-healing Poly(aryl ether sulfone)

Robust Interval State Estimation for Distribution Systems Considering Pseudo-measurement Interval Prediction

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Journal of Modern Power Systems and Clean Energy 2024年第1期12卷 179-188页

作者： Xu Zhang Wei Yan Meiqing huo Hui Li the School of Electrical Engineering Chongqing UniversityChongqing 400044China

Interval state estimation(ISE)can estimate state intervals of power systems according to confidence intervals of predicted pseudo-measurements,thereby analyzing the impact of uncertain pseudo-measurements on ***,predicted pseudo-measurements have prediction errors,and their confidence intervals do not necessarily contain the truth values,leading to estimation biases of the *** solve this problem,this paper proposes a pseudo-measurement interval prediction framework based on the Gaussian process regression(GPR)model,thereby improving the prediction accuracy of pseudo-measurement confidence ***,a weight assignment strategy for improving the robustness of weighted least squares(WLS)ISE is *** strategy quantifies the deviation between the pseudo-measurement intervals and their estimated intervals and assigns smaller weights to the pseudo-measurement intervals with larger deviations,thereby improving the estimation accuracy and robustness of the *** paper adopts the data from the supervisory control and data acquisition(SCADA)system of the New York Independent System Operator(NYISO).It verifies the advantages of the GPR method for pseudo-measurement interval prediction by comparing it with the quantile regression and neural network *** addition,this paper demonstrates the effectiveness of the proposed weight assignment strategy through the IEEE 14-bus ***,the differences in the estimation accuracy and the bad data identification between the robust interval state estimation and deterministic state estimation are discussed.

关键词： Interval state estimation interval analysis pseudo-measurement Gaussian process regression(GPR)

Rotor Angle Stability Prediction Using Temporal and Topological Embedding Deep Neural Network Based on Grid-informed Adjacency Matrix

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Journal of Modern Power Systems and Clean Energy 2024年第3期12卷 695-706页

作者： Peiyuan Sun Long huo Xin Chen Siyuan Liang the School of Electrical Engineering Xi’an Jiaotong UniversityXi’an 710049China the School of Electrical Engineering and the Center of Nanomaterials for Renewable EnergyState Key Laboratory of Electrical Insulation and Power EquipmentXi’an Jiaotong UniversityXi’an 710049China the Department of Computer Science and Engineering The Chinese University of Hong KongShatin999077Hong KongChina

Rotor angle stability(RAS)prediction is critically essential for maintaining normal operation of the interconnected synchronous machines in power *** wide deployment of phasor measurement units(PMUs)promotes the development of data-driven methods for RAS *** paper proposes a temporal and topological embedding deep neural network(TTEDNN)model to accurately and efficiently predict RAS by extracting the temporal and topological features from the PMU *** grid-informed adjacency matrix incorporates the structural and electrical parameter information of the power *** the small-signal RAS with disturbance under initial operating conditions and the transient RAS with short circuits on transmission lines are *** studies of the IEEE 39-bus and IEEE 300-bus power systems are used to test the performance,scalability,and robustness against measurement uncertainties of the TTEDNN *** show that the TTEDNN model performs best among existing deep learning ***,the superior transfer learning ability from small-signal RAS conditions to transient RAS conditions has been proved.

关键词： Rotor angle stability prediction topological embedding deep learning graph convolution network