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Zeylenone promotes apoptosis of chronic myelogenous leukemia-derived K562 cells by a mechanism involving Jak2 and src kinase

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中国药理学与毒理学杂志 2016年第10期30卷 1069-1070页

作者： huo xiao-wei LIAO Yong-hong TIAN Yu GAO Li LIU Dong-yu LI Li-yong CAO Li Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College

OBJECTIVE The present study was designed to investigate anticancer effect of zeylenone(Zey)on K562 cells derived from chronic myelogenous leukemia(CML)both in vitro and in vivo,followed by exploring the underlying *** Initially,the effects of Zey on cel viability,proliferation,and apoptosis were measured in K562 cells by MTT,soft agar assay,AO/EB staining,hoechst 33258 staining and flow cytometric analysis after they were treated with Zey for indicated time,the involving signaling pathways were then investigated by JC-1,real-time quantitative polymerase chain reaction(RT-q PCR),Western blotting and immunofluorescence ***,the in vivo anti-tumoractivity of Zey was assessed with nude xenografts and the involving mechanism was confirmed by immunohistochemical(IHC)and histopathological *** We identified that Zey dose-dependently decreased cell viability,colony formation and expression of Proliferating Cell Nuclear Antigen(PCNA),and significantly induced K562 cell apoptosis via regulating Bcl-2 family members,decreasing mitochondrial transmembrane potential,and activating caspase-3,caspase-9,and caspase-8(P<0.05 or P<0.01).Further study revealed that Zey significantly inhibited phosphorylation of Jak2 and Src and downregulated their downstream proteins,including stat3,PI3K/AKT/m TOR,and ERK1/2 signaling pathways(P<0.05 or P<0.01).Zey also suppressed tumor growth with low toxicity in mouse xenograft model of K562cells through decreasing expression of Jak2 and *** Our data demonstrated that Zey substantially suppressed K562 cells both in vitro and in vivo through Jak2 and Src *** findings suggest the potential of Zey as an effective anticancer agent in CML treatment.

关键词： zeylenone apoptosis K562 cells Jak2 Src

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MreBCD-associated Cytoskeleton is Required for Proper Segregation of the Chromosomal Terminus during the Division Cycle of Escherichia Coli

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Chinese Medical Journal 2015年第9期128卷 1209-1214页

作者： Yu-Jia huo Ling Qiao xiao-wei Zheng Cheng Cui Yuan-Fang Ma Feng Lu Henan Engineering Laboratory of Antibody Medicine Medical School of Henan University Kaifeng Henan 475001 China

Background：In prokaryotic organisms,the mechanism responsible for the accurate partition of newly replicated chromosomes into daughter cells is incompletely *** of the replication terminus of the circular prokaryotic chromosome poses special problems that have not previously been *** aim of this study was to investigate the roles of several protein components （MreB,MreC,and MreD） of the prokaryotic cytoskeleton for the faithful transmission of the chromosomal terminus into daughter ***：Strain LQ1 （mreB：：cat）,LQ2 （mreC：：cat）,and LQ3 （mreD：：cat） were constructed using the Red recombination ***11/pLAU53,LQ12/pLAU53,LQ13/pLAU53,LQ14/pLAU53,and LQ15/pLAU53 strains were generated by Pltransduction of （tetO）240-Gm and （lacO）240-Km cassettes from strains IL2 and *** microscopy was performed to observe localization pattern of fluorescently-labeled origin and terminus foci in wild-type and mutant *** induction was monitored as gfp fluorescence from PsulA-gfp in log phase cells grown in Luria-Bertani medium at 37℃ by measurement of emission at 525 nm with excitation at 470 nm in a microplate fluorescence ***：Mutational deletion of the mreB,mreC,or mreD genes was associated with selective loss of the terminus region in approximately 40％ of the cells within growing *** was accompanied by significant induction of the SOS DNA damage response,suggesting that deletion of terminus sequences may have occurred by chromosomal cleavage,presumably caused by ingrowth of the division septum prior to segregation of the replicated ***：These results imply a role for the MreBCD cytoskeleton in the resolution of the final products of terminus replication and/ or in the specific movement of newly replicated termini away from midcell prior to completion of septal *** would identify a previously unrecognized stage in the overall process of chromosome segregation.

关键词： Cytoskeleton Escherichia Coli MreBCD Segregation of Chromosomal Terminus

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一株绵羊肺炎支原体的分离及鉴定

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中国生物制品学杂志 2017年第11期30卷 1153-1156页

作者：邓守全霍晓伟内蒙古民族大学动物科学与技术学院

目的从疑似患有传染性胸膜肺炎的绵羊肺脏中分离支原菌株,并进行鉴定。方法采用组织浸泡法分离支原体,经革兰染色及瑞氏染色后于显微镜下观察,分离株命名为TLMY 1;经通用引物对分离株16S r DNA基因进行PCR扩增及序列分析,并建立亲缘关... 详细信息

目的从疑似患有传染性胸膜肺炎的绵羊肺脏中分离支原菌株,并进行鉴定。方法采用组织浸泡法分离支原体,经革兰染色及瑞氏染色后于显微镜下观察,分离株命名为TLMY 1;经通用引物对分离株16S r DNA基因进行PCR扩增及序列分析,并建立亲缘关系进化树。结果镜下观察可见分离株TLMY 1的菌落形态呈"煎蛋样",革兰染色为阴性,瑞氏染色为紫红色形态不规则菌体;分离株TLMY 1与Mycoplasma arginini strain C1、787、D17等亲缘性最近。结论分离株TLMY 1为精氨酸支原体。

关键词：绵羊肺炎支原体分离培养 16S r DNA PCR

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刮板输送机断链监测技术研究与系统开发

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机械工程与自动化 2020年第2期 218-219页

作者：霍晓伟长治三元中能煤业有限公司山西长治046605

为进一步提高刮板输送机的故障监测水平,降低故障停机时间,首先对断链故障发生的原因进行了分析,然后分别介绍了3种断链检测方法。在此基础上,提出了基于刮板水平度检测和电机电流检测的断链故障检测系统,并对系统的组成结构和控制逻辑... 详细信息

为进一步提高刮板输送机的故障监测水平,降低故障停机时间,首先对断链故障发生的原因进行了分析,然后分别介绍了3种断链检测方法。在此基础上,提出了基于刮板水平度检测和电机电流检测的断链故障检测系统,并对系统的组成结构和控制逻辑规则进行了研究。

关键词：刮板输送机断链监测

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SHS法反应合成Al_3Ti/Al复合材料的研究

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航空材料学报 2007年第5期27卷 71-76页

作者：霍小卫王树奇陈康敏江苏大学材料科学与工程学院江苏镇江212013

通过将5Al-Ti体系混合粉末预制块在一定温度下预热,原位制备出Al3Ti/Al复合材料,并探讨了其生成机理和影响因素。研究发现:Al粉和Ti粉混合预热生成Al3Ti是由Al粉的熔化诱导;在高于热爆起始温度下预热时,Al3Ti直接以热爆反应机制生成;在... 详细信息

通过将5Al-Ti体系混合粉末预制块在一定温度下预热,原位制备出Al3Ti/Al复合材料,并探讨了其生成机理和影响因素。研究发现:Al粉和Ti粉混合预热生成Al3Ti是由Al粉的熔化诱导;在高于热爆起始温度下预热时,Al3Ti直接以热爆反应机制生成;在低于热爆起始温度下预热时,Al3Ti按扩散-热爆反应机制生成。预热温度、Ti粉粒度及冷却速度是影响材料组织性能的关键因素。通过适当工艺制备出具有性能优异的Al3Ti/Al复合材料,其硬度是铝基体的3.6倍,耐磨性高。

关键词： SHS 反应合成 Al3Ti/Al 热爆反应

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可重构嵌入式软件缺陷优化预测仿真研究

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计算机仿真 2018年第8期35卷 443-447页

作者：霍小卫刘江坡郑州大学西亚斯国际学院河南新郑451150

可重构嵌入式软件优化预测是提高软件测试效率、保证软件运行可靠性的重要手段。针对当前模型没有充分考虑软件缺陷特征历史数据的类不平衡特性,导致预测结果存在收敛速度较慢、训练误差较大的问题。提出一种基于PSO-BP算法的可重构嵌... 详细信息

可重构嵌入式软件优化预测是提高软件测试效率、保证软件运行可靠性的重要手段。针对当前模型没有充分考虑软件缺陷特征历史数据的类不平衡特性,导致预测结果存在收敛速度较慢、训练误差较大的问题。提出一种基于PSO-BP算法的可重构嵌入式软件缺陷优化预测模型,充分考虑可重构嵌入式软件缺陷历史数据的类不平衡性特点,通过确定抽样度,执行欠抽样操作,缓解数据的不平衡性;同时对软件项目开发人员的能力、软件缺陷特征的数量、软件缺陷密度进行计算,并对得到的结果进行k-均值聚类;基于软件缺陷特征数据的处理结果建立具有三层网络结构的BP神经网络预测模型;运用粒子群优化算法优化BP神经网络预测模型的权值和阈值,建立可重构嵌入式软件缺陷优化预测的PSO-BP预测模型。仿真对比测试结果证明,PSO-BP预测模型与BP神经网络预测模型相比,具有更快的收敛速度,且预测结果与真实值更加接近。

关键词：可重构嵌入式软件缺陷预测

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New background quasars in the vicinity of the Andromeda Galaxy discovered with the Guoshoujing Telescope (LAMOST)

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Research in Astronomy and Astrophysics 2010年第7期10卷 612-620页

作者： Zhi-Ying huo xiao-wei Liu Hai-Bo Yuan Hui-Hua Zhang Yong-Heng Zhao Jian-Jun Chen Zhong-Rui Bai Hao-Tong Zhang Hua-wei Zhang Ruben Garcia-Benito Mao-Sheng Xiang Hong-Liang Yan Juan-Juan Ren Shi-wei Sun Yong Zhang Ye-Ping Li Qi-Shuai Lu You Wang Ji-Jun Ni Hai Wang Department of Astronomy Peking University Beijing 100871 China Kavli Institute for Astronomy and Astrophysics Peking University Beijing 100871 China National Astronomical Observatories Chinese Academy of Sciences Beijing 100012 China Department of Astronomy Beijing Normal University Beijing 100875 China Nanjing Institute of Astronomical Optics ＆ Technology Chinese Academy of Sciences Nanjing 210042 China

We present preliminary analyses of spectra of quasar candidates in two Guoshoujing Telescope （GSJT, formerly named the Large Sky Area Multi-Object Fiber Spectroscopic Telescope - LAMOST） test fields near M 31 where one is close to the optical center of the disk and the other is towards the northeastern outskirts of the halo, obtained during the early stage of the GSJT commissioning in the last season of 2009. Both fields contain background low-redshift quasar candidates selected from the SDSS photometry. In total, 14 new quasars with redshifts up to 2 and i magnitudes between 16.7 and 19.2, are discovered, including 7 within the 2.5° central region of M 31. We briefly discuss the potential applications of these newly discovered bright quasars.

关键词： galaxies individual (M31) -- quasars general -- quasars emission lines

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Prospects of elafibranor in treating alcohol-associated liver diseases

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World Journal of Gastroenterology 2025年第2期31卷 132-137页

作者： wei-Tong Cui Hua-Ru Xue Dian-Fang wei xiao-Yu Feng Kai Wang School of Basic Medicine Qilu Medical UniversityZibo 255300Shandong ProvinceChina School of Medical Imaging Qilu Medical UniversityZibo 255300Shandong ProvinceChina

Alcohol-related liver disease(ALD),which is induced by excessive alcohol con-sumption,is a leading cause of liver-related morbidity and *** pa-tients exhibit a spectrum of liver injuries,including hepatic steatosis,inflam-mation,and fibrosis,similar to symptoms of nonalcohol-associated liver diseases such as primary biliary cholangitis,metabolic dysfunction-associated steatotic liver disease,and nonalcoholic *** has been approved for the treatment of primary biliary cholangitis and has been shown to improve symptoms in both animal models and in vitro cell models of metabolic dysfunc-tion-associated steatotic liver disease and nonalcoholic ***,the efficacy of elafibranor in treating ALD remains *** this article,we comment on the recent publication by Koizumi et al that evaluated the effects of elafibranor on liver fibrosis and gut barrier function in an ALD mouse *** findings indicate the potential of elafibranor for ALD treatment,but further experimental investigations and clinical trials are warranted.

关键词： Elafibranor Alcohol-associated liver diseases Peroxisome proliferator-activated receptor Lipid Apoptosis Steatosis Inflammation Fibrosis

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水性颜料体系无树脂色浆的制备与应用

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应用化学 2021年第2期38卷 212-219页

作者：吴振江霍晓伟刘守军杨颂大同英可奥化工技术有限公司大同037500 太原理工大学化学化工学院太原030024 太原英可奥笔墨科技有限公司太原030032

中性墨水属于热力学上不稳定的颜料悬浮体系,选择低黏度、高稳定性的色浆是保证墨水体系分散稳定性的重要手段之一。基于此,以颜料炭黑和酞菁蓝为着色剂,配合超分散剂(EK43)与协同增效剂(BM10),制备了两款适用于中性墨水体系的无树脂色... 详细信息

中性墨水属于热力学上不稳定的颜料悬浮体系,选择低黏度、高稳定性的色浆是保证墨水体系分散稳定性的重要手段之一。基于此,以颜料炭黑和酞菁蓝为着色剂,配合超分散剂(EK43)与协同增效剂(BM10),制备了两款适用于中性墨水体系的无树脂色浆。首次从色浆粒径与体系分散稳定性角度出发,确定了EK43、BM10用量以及最佳研磨时间,并对其理化性能、稳定性与书写性能进行分析测试。结果表明:在黑色浆中添加质量分数10.0%的EK43、2.5%的BM10,研磨时间为90 min;在蓝色浆中添加质量分数8.0%的EK43、2.0%的BM10,研磨时间为120 min时,两种色浆的粒径与分散稳定性均达到最佳。所制备的无树脂色浆颜料固含量高、黏度较低、储存稳定性好、着色力强,以其调配的中性墨水书写性能良好,且具有较好的离心稳定性和耐热稳定性。

关键词：无树脂色浆研磨时间分散稳定性颜料固含量着色力书写性能

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Association between autoimmune gastritis and gastric polyps:Clinical characteristics and risk factors

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 73-87页

作者： Jing-Zheng Jin xiao Liang Shu-Peng Liu Rui-Lan Wang Qing-wei Zhang Yu-Feng Shen xiao-Bo Li Division of Gastroenterology and Hepatology Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China Division of Gastroenterology and Hepatology Shanghai Institute of Digestive DiseasesShanghai 200001China Division of Gastroenterology and Hepatology Renji Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200127China Division of Gastroenterology and Hepatology Sichuan Armed Police Corps HospitalLeshan 610041Sichuan ProvinceChina Division of Gastroenterology and Hepatology NHC Key Laboratory of Digestive DiseasesRenji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China Department of Gastroenterology Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai Institute of Digestive DiseasesShanghai 200127China

BACKGROUND The relationship between autoimmune gastritis(AIG)and gastric polyps(GPs)is not well *** To explore the clinical characteristics and risk factors of AIG with GPs in *** This double center retrospective study included 530 patients diagnosed with AIG from July 2019 to July *** collected clinical,biochemical,serological,and demographic data were of each *** regression analyses,both multivariate and univariate,were conducted to pinpoint independent risk factors for GPs in patients with AIG *** operating characteristic curves were utilized to establish the optimal cutoff values,sensitivity,and specificity of these risk factors for predicting GPs in patients with *** Patients with GPs had a higher median age than those without GPs[61(52.25-69)years vs 58(47-66)years,P=0.006].The gastrin-17 levels were significantly elevated in patients with GPs compared with those without GPs[91.9(34.2-138.9)pmol/mL vs 60.9(12.6-98.4)pmol/mL,P<0.001].Additionally,the positive rate of parietal cell antibody(PCA)antibody was higher in these patients than in those without GPs(88.6%vs 73.6%,P<0.001).Multivariate and univariate analyses revealed that PCA positivity[odds ratio(OR)=2.003,P=0.017],pepsinogen II(OR=1.053,P=0.015),and enterochromaffin like cells hyperplasia(OR=3.116,P<0.001)were significant risk factors for GPs,while pepsinogen I was identified as a protective *** PCA positivity and enterochromaffin like cells hyperplasia are significant risk factor for the development of GPs in patients with *** gastrin-17 levels may also play a role in this *** findings suggest potential targets for further research and therapeutic intervention in managing GPs in patients with AIG.

关键词： Autoimmune gastritis Gastric polyps Neuroendocrine tumor Risk factors Nomogram

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