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烟草质子泵互作蛋白NTPpi1基因的克隆及其生物信息学分析

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分子植物育种 2024年第12期22卷 3871-3876页

作者：霍光吴珊陶德欣李辉邸慧慧湖北省烟草公司恩施州公司恩施445000 湖南文理学院生命与环境科学学院常德415000

为了能够更好地选育高钾烟草品种,本研究根据烟叶钾元素含量全基因组关联分析结果,克隆了烟草质子泵互作蛋白NTPpi1的基因序列。生物信息学分析发现,NTPpi1基因长度为1834 bp,开放读码框为981 bp,编码的蛋白质序列由326个氨基酸组成;蛋... 详细信息

为了能够更好地选育高钾烟草品种,本研究根据烟叶钾元素含量全基因组关联分析结果,克隆了烟草质子泵互作蛋白NTPpi1的基因序列。生物信息学分析发现,NTPpi1基因长度为1834 bp,开放读码框为981 bp,编码的蛋白质序列由326个氨基酸组成;蛋白质分子量为145.3 kD,蛋白质等电点为5.00;通过在线软件TMHMM 2.0 Server预测发现,该蛋白存在一个跨膜的螺旋结构,且跨膜长度有23个氨基酸。氨基酸序列多序列比对分析结果显示,NTPpi1的氨基酸序列与其他植物Ppi1氨基酸序列的一致性为80.82%。通过NetPhos 3.1 Server软件对质子泵互作蛋白NTPpi1的磷酸化位点进行预测,发现有29个可以磷酸化的位点,其中第321位的Threonine磷酸化位点位于跨膜区域,推测这个位点可能是该蛋白与ATP酶质子泵相互作用的位点。以上结果的发现为高钾烟草品种的选育提供一定的理论支持。

关键词：烟草钾元素质子泵互作蛋白

Phosphorus status and microbial community of paddy soil with the growth of annual ryegrass (Lolium multiflorum Lam.) under different phosphorus fertilizer treatments

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第10期10卷 761-768页

作者： Hai-chao GUO guang-huo WANG Department of Resource Science College of Environmental and Resource Sciences Zhejiang University Hangzhou 310029 China

Annual ryegrass （Lolium multiflorum Lam.） was grown in paddy soil in pots under different phosphorus （P） fertilizer treatments to investigate changes of P fractions and microbial community of the soil. The treatments included Kunyang phosphate rock （KPR） applications at 50 mg P/kg （KPRs0） and 250 mg P/kg （KPR250）, mono-calcium phosphate （MCP） application at 50 mg P/kg （MCP50）, and the control without P application. The results showed that KPR50, KPR250, and MCP50 applications significantly increased the dry weight of the ryegrass by 13%, 38%, and 55%, and increased P uptake by 19%, 135%, and 324%, respectively. Compared with MCP50, the relative effectiveness of KPR50 and KPR250 treatments in ryegrass production was about 23% and 68%, respectively. After one season ofryegrass growth, the KPR50, KPR250, and MCP50 applications increased soil-available P by 13.4%, 26.8%, and 55.2%, respectively. More than 80% of the applied KPR-P remained as HC1-P fraction in the soil. Phospholipid fatty acid （PLFA） analysis showed that the total and bacterial PLFAs were significantly higher in the soils with KPR250 and MCP50 treatments compared with KPR50 and control. The latter had no significant difference in the total or bacterial PLFAs. The KPR50, KPR250, and MCP50 treatments increased fungal PLFA by 69%, 103%, and 69%, respectively. Both the principal component analysis and the cluster analysis of the PLFA data suggest that P treatments altered the microbial community composition of the soils, and that P availability might be an important contributor to the changes in the microbial community structure during the ryegrass growth in the paddy soils.

关键词： Phosphorus fractionation Phospholipid fatty acid (PLFA) Ryegrass Phosphate rock

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钾霞石制备体系碱性滤液的利用技术

岩石矿物学杂志 2024年第1期43卷 125-130页

作者：路华龙霍光祥段永华勾明雷刘军娜刘晨河南科技大学化学化工学院河南洛阳471000

为处理钾长石水热制备钾霞石所产生的碱性滤液,本文采用水热法,考察了氢氧化铝溶解时间、晶化时间、晶化温度、水碱比对钾霞石产率和白度的影响,并对合成钾霞石物相进行了表征。结果表明,合成钾霞石的最佳条件为,氢氧化铝溶解时间为1.5... 详细信息

为处理钾长石水热制备钾霞石所产生的碱性滤液,本文采用水热法,考察了氢氧化铝溶解时间、晶化时间、晶化温度、水碱比对钾霞石产率和白度的影响,并对合成钾霞石物相进行了表征。结果表明,合成钾霞石的最佳条件为,氢氧化铝溶解时间为1.5 h,晶化时间为4 h,晶化温度280℃,水碱比为1.8。XRD结果表明,产物为钾霞石粉体。傅里叶变换红外光谱表明,Al(OH)_(3)中的Al在水热条件下进入到Si—O骨架中形成了Si—O—Al官能团,从而印证了钾霞石的合成。差热分析结果表明,合成钾霞石具有良好的热稳定性。氮气吸附结果表明,合成钾霞石比表面积为5.18 m^(2)/g,平均孔径为32.98 nm。实现了钾长石水热制备钾霞石所剩碱性滤液的资源化利用,并为钾长石水热制备钾霞石提供了一种母液循环的思路,使水热制备钾霞石工业化成为一种可能。

关键词：钾霞石水热法氢氧化铝

Phospholipid fatty acid patterns of microbial communities in paddy soil under different fertilizer treatments

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Journal of Environmental Sciences 2007年第1期19卷 55-59页

作者： ZHANG Qi-chun WANG guang-huo YAO Huai-ying Department of Natural Resources Zhejiang University Hangzhou 310029 China

The microbial communities under irrigated rice cropping with different fertilizer treatments, including control （CK）, PK, NK, NP, NPK fertilization, were investigated using phospholipid fatty acid （PLFA） profile method. The results of this study revealed that the fertilizer practice had an impact on the community structure of specific microbial groups. The principal components analysis （PCA） showed that proportion of the actinomycete PLFAs （10Me 18：0 and 10Me 16：0） were the lowest in the PK treatment and the highest in the NPK treatment, which means that soil nitrogen status affected the diversity of actinomycetes, whereas nitrogen cycling was related to the actinomycets. Under CK treatment, the ratio of Gram-positive to Gram-negative bacteria was lower compared with that in fertilizer addition treatments, indicating that fertilizer application stimulated Gram-positive bacterial population in paddy soil. The fatty acid 18：2to6,9, which is considered to be predominantly of fungal origin, was at low level in all the treatments. The ratio of cyl9：0 to 18：1 to7, which has been proposed as an indicator of stress conditions, decreased in PK treatment. Changes of soil microbial community under different fertilizer treatments of paddy soil were detected in this study; however, the causes that lead to changes in the microbial community still needs further study.

关键词： paddy soil fertilizers soil microorganisms community structure PLFA PCA

Liver metabonomics study on the protective effect of glycyrrhetinic acid against realgar-induced liver injury

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Chinese Journal of Natural Medicines 2020年第2期18卷 138-147页

作者： huo Tao-guang FANG Ying ZHANG Ying-Hua FENG Cong JIANG Hong Department of Health Laboratory Technology School of Public HealthChina Medical UniversityShenyang 110122China

Glycyrrhetinic acid(GA)is the bioactive ingredient in Glycyrrhizae Radix et *** previous study has reported that GA has protective effect on realgar-induced ***,the details of the hepatoprotective mechanisms of GA on realgar-induced liver injury remain to be *** the study,mice were divided into control,GA-control,realgar,and co-treated *** liver tissues were used for metabonomics study by ultra-performance liquid chromatography-mass spectrometry(UPLCMS)*** results illustrate that GA significantly ameliorate the liver injury and metabolic perturbations caused by *** metabolites,such as phenylalanine,pyroglutamic acid(PGA),proline,carnitine,nicotinamide,choline,lysophosphatidylcholine(LPC)16:0 and LPC 18:2 were found responsible for the hepatoprotective effect of *** metabolites are associated with the methylation metabolism of arsenic,cell membrane structure,energy metabolism and oxidative *** the results of this study,we infer that the potential hepatoprotective mechanism of GA on realgar-induced liver injury may be associated with reducing arsenic accumulation and its methylation metabolism in the liver,promoting the conjugation of arsenic and GSH to play detoxification effect,and ameliorating the liver metabolic perturbations caused by realgar.

关键词： Metabonomics Glycyrrhetinic acid Realgar Liver UPLC-MS

Soil Organic N Forms and N Supply as Affected by Fertilization Under Intensive Rice Cropping System

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Pedosphere 2006年第3期16卷 345-353页

作者： ZHANG Qi-Chun WANG guang-huo XIE Weng-Xia College of Environmental and Natural Resources Science Zhejiang University Hangzhou 310029 （China）

Changes of soil organic nitrogen forms and soil nitrogen supply under continuous rice cropping system were investigated in a long-term fertilization experiment in Jinhua, Zhejiang Province, China. The fertilizer treatments included combination of P-K, N-K, N-P, and N-P-K as well as the control. After six years of continuous double-rice cropping, total soil N and hydrolysable N contents remained stable in plots with N treatments, while the hydrolysable N contents were substantially reduced in those plots without N application. Compared to the unbalanced fertilization treatments, P and K increased the percentage of hydrolysable ammonium N in the total soil N with the balanced application of N, and also maintained higher rice grain yields and nitrogen uptake. Grain yield was positively correlated with total N uptake (r = 0.875**), hydrolysable N (r = 0.608**), hydrolysable ammonium N (r = 0.560**) and the hydrolysable unknown N (r = 0.417**). Total N uptake was positively correlated with hydrolysable N (r = 0.608**), hydrolysable ammonium N (r = 0.440**) and hydrolysable unknown N (r = 0.431**). Soil nutrient depletion and/or unbalanced fertilization to rice crop reduced N content in soil microbial biomass, and therefore increased C/N ratio, suggesting a negative effect on the total microbial biomass in the soil.

关键词： continuous cropping microbial biomass organic N forms rice

基于呼吸力学、肺功能变化探究血必净联合氨溴索肺泡灌洗治疗ICU重症肺炎患者的价值

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四川生理科学杂志 2024年第5期46卷 1129-1131页

作者：王光伙郭建国吴艳伶大田县总医院福建三明366100

目的:探究血必净联合氨溴索肺泡灌洗治疗ICU重症肺炎患者呼吸力学、肺功能变化方面的价值。方法:回顾性选取2020年7月至2022年11月在我院ICU采用120 mg盐酸氨溴索肺泡灌洗联合2 g亚胺培南西司他丁钠静脉滴注治疗的30例SP患者作为对照组... 详细信息

目的:探究血必净联合氨溴索肺泡灌洗治疗ICU重症肺炎患者呼吸力学、肺功能变化方面的价值。方法:回顾性选取2020年7月至2022年11月在我院ICU采用120 mg盐酸氨溴索肺泡灌洗联合2 g亚胺培南西司他丁钠静脉滴注治疗的30例SP患者作为对照组。按1:1比例选取采用100 mL血必净静脉滴注联合氨溴索肺泡灌洗治疗的30例SP患者作为试验组。两组疗程均为2 w。在治疗前后采用有创呼吸机测定两组呼吸力学指标,采用肺炎严重指数(PSI)评分和临床肺部感染评分(CPIS)评估两组肺功能变化,采用酶联免疫吸附法测定两组炎性标志物变化。结果:治疗后试验组采用有创呼吸机检测的气道阻力(Raw)、呼气末正压(PEEP)值低于对照组、肺动态顺应性(Cdyn)值均高于对照组(P<0.05);治疗后试验组PSI、CPIS评分均低于对照组(P<0.05)。治疗后试验组采用酶联免疫吸附法测定的炎性标志物水平低于对照组(P<0.05)。结论:血必净联合纤维支气管镜下氨溴索肺泡灌洗可有效改善SP患者呼吸功能,促进其肺功能和炎性指标的恢复。

关键词：血必净肺泡灌洗重症监护室重症肺炎

Potential production simulation and optimal nutrient management of two hybrid rice varieties in Jinhua, Zhejiang Province, China

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第7期8卷 486-492页

作者： XIE Wen-xia WANG guang-huo ZHANG Qi-chun School ofEnvwonmental andResource Science Zhejiang University Hangzhou 310029 China School of Chemistry and Environment Science Qingdao University Qingdao 266071 China

Potential growth of two widely-grown hybrid rice varieties in the Jinhua district of Zhejiang Province, Shanyou63 for mid-rice and Xieyou46 for late rice, was simulated using a crop growth model of WOFOST. Parameters of the rice growth in WOFOST were calibrated through field experiments from 1999 to 2002 in Jinhua. The potential yield simulated with WOFOST was about 12 t/ha for Shanyou63 and 10 t/ha for Xieyou46, which are in good agreement with the highest recorded yield obtained in this area. Under farmers practice, current yield is about 7.5 t/ha for Shanyou63 and 6.5 t/ha for Xieyou46. There is a gap between the actual rice yield and the potential yield for these two hybrid rice varieties grown in this area. The attainable target yields were set to 70% to 75% of their potential yields for the two varieties. A recently developed software "Nutrient Decision Support System (NuDSS)" for irrigated rice was used to optimize nutrient management for these two rice varieties. According to NuDSS, the optimal fertilizer N requirement for the target yields was about 150 kg/ha for Shanyou63 and about 120 kg/ha for Xieyou46, which were only about 70% of the fertilizer N application under current farmers' practice. Comparing with farmers' practice, there is great potential to increase actual rice yields and to reduce fertilizer N use rates by improving rice crop management practice in Jinhua.

关键词： Rice WOFOST NuDSS Potential growth Nitrogen management

圆形预应力钢丝缠绕正交预紧机架的缠绕分析计算

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锻压装备与制造技术 2024年第3期59卷 43-46页

作者：叶龙赵绪平霍光李宣祁建北方重工集团有限公司辽宁沈阳110141

在预应力钢丝缠绕正交预紧机架结构的基础上提出了一种全新的机架型式——圆形预应力钢丝缠绕正交预紧机架。对该型机架的缠绕进行理论分析和计算,进而求得缠绕施工张力表,为钢丝缠绕施工提供重要理论依据。该型机架的研发是超重型承载... 详细信息

在预应力钢丝缠绕正交预紧机架结构的基础上提出了一种全新的机架型式——圆形预应力钢丝缠绕正交预紧机架。对该型机架的缠绕进行理论分析和计算,进而求得缠绕施工张力表,为钢丝缠绕施工提供重要理论依据。该型机架的研发是超重型承载机架结构的重要技术突破。

关键词：预应力钢丝缠绕正交预紧机架圆形