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Screening and identification of cellulose-degradation strain M1 and its characteristics of cellulase

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Journal of Life Sciences 2009年第9期3卷 21-26,33页

作者： huang jin-yong wang Mei-rong wang Bing Department of Bioengineering Zhengzhou University Zhengzhou 450001 China

In this paper, in order to get the target microbe which has high cellulose bio-degradation ability, we collected soil samples from environment rich in cellulose. Carboxymethyl cellulose sodium gel plate and filter paper plate were used for microbe screening, and finally we got a strain named MI which has the highest cellulase producing ability, and it has been identified as Penicilium oxalicum by morphological and molecular biological identification. We have studied cellulose degradation ability of this strain under different conditions such as pH value, temperature and inoculating time. The results showed that CMCase which belongs to acid enzyme, could achieve its maximum enzyme activity 489.96 U/ml when the optimal pH value was 4. The FPAase could achieve its maximum enzyme activity 1,595.45 U/ml when the optimal pH value was 4, too. The optimal ferment temperature of CMCase is 50℃ when achieved the maximum of enzyme activity at the second day, whereas the FPAase could reach its tip top on the third day, So the optimal ferment temperature of FPAase is also 50℃.

关键词： cellulose degradation Penicilium oxalicum screening

Quickly obtaining densely dispersed coherent particles in steel matrix and its related mechanical property

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 111-118页

作者： Xiaoxiao wang Qingsong huang School of Chemical Engineering Sichuan UniversityChengdu 610065China

Densely distributed coherent nanoparticles(DCN)in steel matrix can enhance the work-hardening ability and ductility of steel *** the routes to this end can be generally classified into the liquid-solid route and the solid-solid ***,the formation of DCN structures in steel requires long processes and complex *** far,obtaining steel with coherent particle enhancement in a short time remains a bottleneck,and some necessary steps remain ***,we show a high-efficiency liquid-phase refining process reinforced by a dynamic magnetic ***-Y-Mn-O particles had an average size of around(3.53±1.21)nm and can be obtained in just around 180 *** small nanoparticles were coherent with the matrix,implying no accumulated dislocations between the particles and the steel *** findings have a potential application for improving material machining capacity,creep resistance,and radiation resistance.

关键词： ferritic steels coherent particles microstructure compression test work hardening

Deep learning identification of novel autophagic protein-protein interactions and experimental validation of Beclin 2-Ubiquilin 1 axis in triple-negative breast cancer

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Oncology Research 2025年第1期33卷 67-81页

作者： XIANG LI WENKE jin LIFENG WU HUAN wang XIN XIE WEI huang BO LIU State Key Laboratory of Southwestern Chinese Medicine Resources School of Pharmacy and College of Medical TechnologyChengdu University of Traditional Chinese MedicineChengdu611137China Department of Biotherapy Cancer Center and State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu610041China

Background:Triple-negative breast cancer(TNBC),characterized by its lack of traditional hormone receptors and HER2,presents a significant challenge in oncology due to its poor response to conventional *** is an important process for maintaining cellular homeostasis,and there are currently autophagy biomarkers that play an effective role in the clinical treatment of *** contrast to targeting protein activity,intervention with proteinprotein interaction(PPI)can avoid unrelated crosstalk and regulate the autophagy process with minimal interference ***:Here,we employed Naive Bayes,Decision Tree,and k-Nearest Neighbors to elucidate the complex PPI network associated with autophagy in TNBC,aiming to uncover novel therapeutic ***,the candidate proteins interacting with Beclin 2 were initially screened in MDA-MB-231 cells using Beclin 2 as bait protein by immunoprecipitation-mass spectrometry assay,and the interaction relationship was verified by molecular docking and CO-IP experiments after *** formation,cellular immunofluorescence,cell scratch and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)tests were used to predict the clinical therapeutic effects of manipulating candidate ***:By developing three PPI classification models and analyzing over 13,000 datasets,we identified 3733 previously unknown autophagy-related *** network analysis revealed the central role of Beclin 2 in autophagy regulation,uncovering its interactions with 39 newly identified ***,the CO-IP studies identified the substantial interaction between Beclin 2 and Ubiquilin 1,which was anticipated by our model and discovered in immunoprecipitation-mass spectrometry assay ***,in vitro investigations showed that overexpressing Beclin 2 increased Ubiquilin 1,promoted autophagy-dependent cell death,and inhibited proliferation and metastasis in MDA-MB-231 ***:This study not

关键词： Triple-negative breast cancer(TNBC) Autophagy Protein-protein interactions(PPI) Artificial intelligence(AI) Beclin 2 Ubiquilin 1

Protective effect of membrane cofactor protein against complementdependent injury

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Acta Pharmacologica Sinica 2005年第8期26卷 987-991页

作者： Dong XU, Shou-jian huang~1, jin-qun wang, Chu-kun WU Department of Pharmacology, Sun Yat-Sen Medical College of Sun Yat-Sen University, Guangzhou 510080, China Department of Pharmacology Sun Yat-Sen Medical College of Sun Yat-Sen University Guangzhou China

Aim: To evaluate the protective role of membrane cofactor protein (MCP CD46) on complement-dependent injury. Methods: MCP was separated by ion exchange chromatography on a DEAE sephadex A-50 column from pig erythrocyte ghosts. Its protective effect was tested in models such as cobra venom factor (CVF)-induced platelet metamorphosis and aggregation, human serum-induced injury in isolated working guinea pig heart and reverse passive Arthus reaction. Results: MCP inhibited CVF-induced platelet metamorphosis with an IC50of 56.7 mg/L±2.6 mg/L, and prevented injury induced by activated complement in isolated working guinea pig hearts. In the rat model of reverse Arthus reaction, MCP relieved the skin lesions induced by immune complexes. Conclusion: MCP has a protective effect against complement-dependent injury.

关键词： CD46 antigen complement heart reverse Arthus reaction

The Hilbert expansion of the Boltzmann equation in the incompressible Euler level in a channel

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Science China Mathematics 2025年第1期68卷 39-88页

作者： Feimin huang Weiqiang wang Yong wang Feng Xiao Academy of Mathematics and Systems Science Chinese Academy of Sciences School of Mathematical Sciences University of Chinese Academy of Sciences Key Laboratory of Computing and Stochastic Mathematics (Ministry of Education) School of Mathematics and StatisticsHunan Normal University

The study of the hydrodynamic limit of the Boltzmann equation with physical boundary is a challenging problem due to the appearance of the viscous and Knudsen boundary *** this paper,the hydrodynamic limit from the Boltzmann equation with the specular reflection boundary condition to the incompressible Euler equations in a channel is *** on the multi-scaled Hilbert expansion,the equations with boundary conditions and compatibility conditions for interior solutions,and viscous and Knudsen boundary layers are derived under different scaling,***,some uniform estimates for the interior solutions,and viscous and Knudsen boundary layers are *** the help of the L2-L∞framework and the uniform estimates obtained above,the solutions to the Boltzmann equation are constructed by the truncated Hilbert expansion with multiscales,and hence the hydrodynamic limit in the incompressible Euler level is justified.

关键词： Boltzmann equation incompressible Euler equations hydrodynamic limit Hilbert expansion specular reflection boundary condition viscous boundary layer Knudsen boundary layer

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混沌信号在无线传感器网络中的盲分离

物理学报 2014年第5期63卷 44-51页

作者：黄锦旺冯久超吕善翔华南理工大学电子与信息学院广州510641

混沌信号在本质上属于非线性非高斯信号,它在无线传感器网络下的应用还涉及到信号量化问题,这使得混沌信号在此应用环境下的信号盲分离更为棘手.针对此问题,本文在容积卡尔曼粒子滤波的框架下提出一种解决方法.文中首先推导出观测信号... 详细信息

混沌信号在本质上属于非线性非高斯信号,它在无线传感器网络下的应用还涉及到信号量化问题,这使得混沌信号在此应用环境下的信号盲分离更为棘手.针对此问题,本文在容积卡尔曼粒子滤波的框架下提出一种解决方法.文中首先推导出观测信号的概率密度函数,在量化比特有限的情况下,采用最优量化器,获得最优的量化结果.在此基础上,使用容积卡尔曼滤波器产生粒子滤波中的重要性概率密度函数,融入最新的观测值,提高粒子对系统状态后验概率的逼近,提高信号盲分离的精度.仿真结果表明算法能够有效地分离混合混沌信号,参数估计的精度及其运算量均优于已有的无先导卡尔曼粒子滤波算法,其运行时间为无先导卡尔曼粒子滤波算法的88.77%.

关键词：混沌信号盲分离无线传感器网络粒子滤波器

Local-contrastive-learning machine with both generalization and adversarial robustness: A statistical physics analysis

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 109-123页

作者： Mingshan Xie Yuchen wang Haiping huang PMI Lab School of Physics Sun Yat-sen University Guangdong Provincial Key Laboratory of Magnetoelectric Physics and Devices Sun Yat-sen University

Distinct from human cognitive processing, deep neural networks trained by backpropagation can be easily fooled by adversarial examples. To design a semantically meaningful representation learning, we discard backpropagation, and instead, propose a local contrastive learning, where the representations for the inputs bearing the same label shrink(akin to boson) in hidden layers,while those of different labels repel(akin to fermion). This layer-wise learning is local in nature, being biologically plausible.A statistical mechanics analysis shows that the target fermion-pair-distance is a key parameter. Moreover, the application of this local contrastive learning to MNIST benchmark dataset demonstrates that the adversarial vulnerability of standard perceptron can be greatly mitigated by tuning the target distance, i.e., controlling the geometric separation of prototype manifolds.

关键词： machine learning statistical mechanics adversarial robustness neural networks

Photoinduced radical sulfur dioxide insertion with asymmetric cyclization of alkenes: accessing β-chiral sulfones bearing S-stereocentric cyclic sulfinamides

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Science China Chemistry 2025年第1期68卷 257-263页

作者： Jiapian huang Chenxin wang Xu wang Xiaoyu Zhou Wei Xiao Jie Wu School of Pharmaceutical and Chemical Engineering & Institute for Advanced Studies Taizhou University State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences School of Chemistry and Chemical Engineering Henan Normal University

The generation of various β-chiral sulfones bearing chiral cyclic sulfinamides through photoredox-catalyzed sulfur dioxide insertion with asymmetric radical cyclization is described. This method features a broad substrate scope and excellent stereospecificity. Computational studies reveal that the dispersion interaction between sulfinyl group and CH2SO2Ph group plays a crucial role in the stereoselectivity control.

关键词： β-chiral sulfones chiral cyclic sulfinamides sulfur dioxide insertion asymmetric radical cyclization stereoselectivity

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一种无线传感器网络中的混沌信号重构算法

物理学报 2014年第14期63卷 77-83页

作者：黄锦旺李广明冯久超晋建秀华南理工大学电子与信息学院广州510641 东莞理工学院计算机学院东莞523808

将无线传感器网络节点观测区域中的一个混沌信号发送到融合中心,进行信号重构.由于节点的通信带宽受限,信号传输之前需要进行量化,给信号带来量化噪声,使得信号重构工作变得更为棘手.本文提出用平方根容积卡尔曼滤波器对融合中心收集的... 详细信息

将无线传感器网络节点观测区域中的一个混沌信号发送到融合中心,进行信号重构.由于节点的通信带宽受限,信号传输之前需要进行量化,给信号带来量化噪声,使得信号重构工作变得更为棘手.本文提出用平方根容积卡尔曼滤波器对融合中心收集的信号进行重构.首先估计观测信号的概率密度函数,使用最优量化器量化观测信号,在有限的量化比特数下,取得最优的信号量化性能.平方根容积卡尔曼滤波器相对无先导卡尔曼算法具有较少的求容积分点,因此具有计算量小的优点,同时迭代过程采用传递误差矩阵的平方根矩阵,保证迭代过程的稳定性和提高数据估计精度.仿真结果表明,该算法能够有效和快速地重构观测信号,并且比基于无先导卡尔曼滤波的算法更快.

关键词：求容积分准则概率密度函数信号重构混沌信号