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轻基质类型及配比对闽楠嫩枝扦插生根的影响

南方农业 2024年第9期18卷 144-149,154页

作者：黄雨芹徐振鑫董佳文湖南应用技术学院湖南常德415199

闽楠是中国特有的Ⅱ级濒危保护树种,其野生资源日渐枯竭。扦插繁殖技术具有操作简单、成本低等特点,已逐渐成为闽楠的主要繁殖手段之一。试验设置泥炭、蛭石、河沙和椰糠等4种轻基质及其不同组合配比共10组处理和1组对照(CK),分析不同... 详细信息

闽楠是中国特有的Ⅱ级濒危保护树种,其野生资源日渐枯竭。扦插繁殖技术具有操作简单、成本低等特点,已逐渐成为闽楠的主要繁殖手段之一。试验设置泥炭、蛭石、河沙和椰糠等4种轻基质及其不同组合配比共10组处理和1组对照(CK),分析不同基质及其配比对闽楠扦插生根的影响。通过测定闽楠扦插苗的各项生长指标,并进行主成分分析及综合评价,筛选出进行闽楠扦插的理想基质。结果表明:1)不同轻基质处理对闽楠扦插的生根率、生根数、最大根长、最大根粗、根鲜重和抽梢高均有显著影响;扦插所使用的基质不同,闽楠扦插生根表现各异;泥炭100%基质扦插的生根率、最大根长、最大根粗和抽梢高这4个指标中排第1位,分别为71.11%、5.9 cm、1.21 mm、6.74 cm;泥炭50%+河沙50%基质扦插的生根数和根鲜重最大,泥炭100%基质次之。2)主成分分析方法综合评价显示泥炭100%基质的扦插效果最佳,河沙100%基质最差。3) 11组基质在容重、总孔隙度、通气孔隙度、毛管孔隙度、pH值和电导率这些理化性质均有显著性差异。4)通过对基质理化性质和扦插生根指标进行典型性相关分析,可得出基质的总孔隙度对扦插生根的影响最大,其次是通气孔隙度及容重。

关键词：闽楠基质不同配比扦插生根

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一株副干酪乳杆菌的抗炎抑菌作用分析

四川动物 2024年第2期43卷 186-194页

作者：康学娜尚可黄文英范振鑫岳碧松四川大学生命科学学院生物资源与生态环境教育部重点实验室成都610065 云南省迪庆州香格里拉市金江镇农业综合服务中心云南香格里拉674403

副干酪乳杆菌Lactobacillus paracasei是重要的益生菌之一。从贵州酸菜中分离得到的11株副干酪乳杆菌中筛选得到一株编号为GZ0613的菌株,体外表型研究显示,该菌株不溶血,对四环素等4种抗生素均表现为敏感,具有较好的耐酸耐盐能力和动物... 详细信息

副干酪乳杆菌Lactobacillus paracasei是重要的益生菌之一。从贵州酸菜中分离得到的11株副干酪乳杆菌中筛选得到一株编号为GZ0613的菌株,体外表型研究显示,该菌株不溶血,对四环素等4种抗生素均表现为敏感,具有较好的耐酸耐盐能力和动物胃肠环境适应能力,对结肠癌细胞HT-29具有较强的黏附能力,对多种常见动物肠道致病菌有较强的生长抑制活性。通过葡聚糖硫酸钠(DSS)诱导建立小鼠结肠炎模型,比较空白对照组、DSS模型组与益生菌处理组小鼠炎症因子水平和炎症浸润程度、解剖学特征及肠道菌群结构之间的差异,结果表明,益生菌处理组小鼠血清中4个炎症因子IL-1β、IL-6、IL-8和TNF-α指标明显降低,脏器指数、结直肠长度及肠道菌群丰度和多样性更趋近于正常小鼠,说明该菌对DSS诱导的小鼠结肠炎有预防缓解作用,具有益生潜力,在动物功能性食品中具有应用前景。

关键词：贵州酸菜副干酪乳杆菌益生性 DSS诱导结肠炎

Structure and function of the membrane microdomains in osteoclasts

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Bone Research 2023年第4期11卷 665-678页

作者： Jialong Hou Jian Liu Zhixian huang Yining Wang Hanbing Yao zhenxin Hu Chengge Shi Jiake Xu Qingqing Wang Department of Orthopaedic Surgery Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhouChina Department of Orthopaedics The First Affiliated Hospital of Wenzhou Medical UniversityWenzhouChina Department of Spine Surgery Peking University Fourth School of Clinical MedicineBeijingChina School of Biomedical Sciences The University of Western AustraliaPerthWAAustralia Shenzhen Institute of Advanced Technology Chinese Academy of SciencesShenzhenChina

The cell membrane structure is closely related to the occurrence and progression of many metabolic bone diseases observed in the clinic and is an important target to the development of therapeutic strategies for these diseases.Strong experimental evidence supports the existence of membrane microdomains in osteoclasts(OCs).However,the potential membrane microdomains and the crucial mechanisms underlying their roles in OCs have not been fully characterized.Membrane microdomain components,such as scaffolding proteins and the actin cytoskeleton,as well as the roles of individual membrane proteins,need to be elucidated.In this review,we discuss the compositions and critical functions of membrane microdomains that determine the biological behavior of OCs through the three main stages of the OC life cycle.

关键词： domains function elucidated

Three-dimensional Au/carbon nanotube-graphene foam hybrid nanostructure for dendrite free sodium metal anode with long cycle stability

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Journal of Materials Science & Technology 2022年第23期118卷 128-136页

作者： zhenxin huang Zixuan Wang Bofang Tian Tingting Xu Caiyun Ma Zhuangfei Zhang Jinhao Zang Dezhi Kong Xinjian Li Ye Wang Key Laboratory of Material Physics Ministry of EducationSchool of Physics and MicroelectronicsZhengzhou UniversityZhengzhou 450052China

Sodium metal anode has been attracting widely research attention due to its large capacity and low electrode potential as the anode of sodium-ion batteries.However,the uncontrollable growth of Na dendrite is one of the critical issues for its real applications.Herein,a three-dimensional(3 D) nanostructure composed of gold nanoparticles(Au NPs) supported on 3 D carbon nanotube-graphene foam(3 D CNT-GF)was designed and fabricated as the host of sodium metal anode.Na@3 D Au/CNT-GF anode can deliver a Coulombic efficiency of 99.14% and stably cycle for 2600 h at 1 mA cm^(-2) with 1 mAh cm^(-2).It can cycle for 300 h at 5 mA cm^(-2) with 1 mAh cm^(-2).Detailed results indicate that its excellent electrochemical performance can be attributed to the unique macroporous structure and sodiophilic surface formed by Au NPs guiding the uniform sodium metal deposition enabled a dendrite-free morphology investigated by the ex-situ SEM and in-situ optical microscopy.At last,a full cell was assembled with Na@3 D Au/CNT-GF as the anode and Na_(3) V_(2)(PO_(4))_(3)@C as the cathode.It can deliver a capacity of 84.6 mAh g^(-1) at 100 mA g^(-1)after 200 cycles.Our results demonstrate that 3 D Au/CNT-GF is a promising sodium metal anode host.

关键词： 3D Au/CNT-GF Sodiophilic Au NPs Dendrite free Uniform deposition In-situ optical microscopy investigation

Isolation,Characterization and Phylogenetic Analysis of Stagonospora tainanensis,the Pathogen Causing Sugarcane Leaf Blight in China

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Isolation,Characterization and Phylogenetic Analysis of Stag...

Three-dimensional Ag/carbon nanotube-graphene foam for high performance dendrite free lithium/sodium metal anodes

中国植物病理学会2023年学术年会

作者： huang zhenxin SHI Qian ZENG Quan LIANG Haoming YU Quan MENG Jiaorong CHEN Baoshan State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources

Sugarcane leaf blight(SLB),a major fungal leaf disease of sugarcane(Saccharum spp.),has been attributed to Stagonospora tainanensis.In December 2020 and May 2021,signs of leaf blight were observed on sugarcane in the fields of Chongzuo City,in the Guangxi Province of China.Lesions on the leaves were characterized by yellow or dark red spots in the center.Fungal species were isolated,purified and subjected to pathogenicity evaluation on the sugarcane plants.An isolate that caused symptoms the same as those observed in the field was initially identified as S.tainanensis(Leptosphaeria taiwanensis,perfect state) based on its morphological characteristics both of asexual and sexual stages.Dark brown and nearly spherical pycnidia with conidia of long ellipsoidal,hyaline,one to four cells and 29.27 to 54.39 μm long and 9.03 to 16.12 μm wide were found on corn meal agar medium.Ascomata with asci of cylindrical to clavate,a short stipe and eight spores slightly constricted at the septum,with the size of the spore ranging from 36 to 44 μm long and 8.5 to 12 μm wide,were formed on the sugarcane-leaf-decoction saccharose agar medium.The identity of the species was further confirmed by rDNA ITS and TEF-1α sequencing.The optimal temperature for mycelial growth was 25℃ and the optimal pH was 6.0.The pathogen grew well in a medium with oats as the carbon source and yeast extract as the nitrogen source,but poorly in a medium with urea as the nitrogen source.This study is the first to identify the sugarcane leaf blight pathogen in Guangxi,and the first publication describing the biological characterization of S.tainanensis.The occurrence of sugarcane leaf blight should alert sugarcane breeders and plant pathologists to consider integrating control of this potentially important disease into the agenda of their breeding and disease control programs.

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Journal of Materials Science & Technology 2023年第1期132卷 50-58页

作者： Bofang Tian zhenxin huang Xilian Xu Xiehong Cao Hui Wang Tingting Xu Dezhi Kong Zhuangfei Zhang Jie Xu Jinhao Zang Xinjian Li Ye Wang Key Laboratory of Material Physics Ministry of EducationSchool of Physics and MicroelectronicsZhengzhou UniversityZhengzhou 450052China College of Materials Science and Engineering Zhejiang University of TechnologyHangzhou 310014China Center of Super-Diamond and Advanced Films(COSDAF)and Department of Chemistry City University of Hong Kong999077Hong Kong SARChina

Although lithium metal and sodium metal are promised as ideal anodes for lithium ion batteries(LIBs)and sodium ion batteries(SIBs),they still suffer from inevitable dendrite growth.In light of this,silver nanoparticles(Ag NPs)are sputtered onto three-dimensional carbon nanotube decorated graphene foam(3D CNT-GF)to construct superior 3D Ag/CNT-GF composite matrix for lithium metal anodes(LMAs)and sodium metal anodes(SMAs).With this design,lithiophilic/sodiophilic Ag NPs could provide favorable sites to guide Li/Na metal nucleation and growth,thus leading to low nucleation overpotentials,high Coulombic efficiency and long cycle performance.Accordingly,3D Ag/CNT-GF electrodes can stably cy-cle for 1000 and 750 cycles at 3 mA cm^(−2)with 1 mAh cm^(−2)for SMAs and LMAs,respectively.More attractively,it can also stably sustain 300 cycles(SMAs)and 500 cycles(LMAs)at a large current den-sity of 5 mA cm^(−2)with 1 mAh cm^(−2).The excellent electrochemical performance can be attributed to the lithiophilic/sodiophilic electrode surface,3D porous electrode structure and the dendrite-free mor-phology as demonstrated by ex-situ scanning electron microscopy(SEM)and in-situ optical microscopy analyses.Furthermore,full cells based on Na@3D Ag/CNT-GF||Na 3 V 2(PO 4)3@carbon(NVP@C)and Li@3D Ag/CNT-GF||LiFePO 4(LFP)could deliver highly reversible capacities of 90.1 and 106.4 mAh g^(−1),respec-tively,at 100 mA g^(−1)after 200 cycles for SIBs and LIBs,respectively.This work demonstrates a novel 3D Ag/CNT-GF matrix for boosting Li/Na deposition stability for their future applications.

关键词： Li/Na metal anodes Lithiophilic/sodiophilic 3D Ag/CNT-GF nanostructure Dendrite-free morphology Uniform deposition In-situ optical microscopy investigation

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有机硅海洋防污涂料的研究进展

有机硅材料 2024年第1期38卷 87-92页

作者：姜宇嵘伞振鑫黄素萄郭洁龙娇秀向威曲鹏飞江西蓝星星火有机硅有限公司江西九江330300 廊坊燕京职业技术学院河北廊坊065200

随着海洋环保意识的提高,环境友好型防污涂料应运而生。本文综述了低表面能防污涂料的防污机理、发展现状以及有机硅防污涂料的分类,重点介绍了有机硅改性树脂防污涂料、仿生有机硅防污涂料以及自析油有机硅防污涂料的研究进展,并对其... 详细信息

随着海洋环保意识的提高,环境友好型防污涂料应运而生。本文综述了低表面能防污涂料的防污机理、发展现状以及有机硅防污涂料的分类,重点介绍了有机硅改性树脂防污涂料、仿生有机硅防污涂料以及自析油有机硅防污涂料的研究进展,并对其发展前景进行了展望。

关键词：有机硅防污涂料改性仿生自析油

Mortality of Dementia and Its Major Subtypes in Urban and Rural Communities of Beijing

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Biomedical and Environmental Sciences 2011年第5期24卷 483-490页

作者： WEN HongBo ZHANG zhenxin huang JueBin DUAN LeiLei WANG QingHua Department of Neurology Peking Union Medical College Hospital Chinese Academy of Medical Sciences Beijing100730 China

Objective To identify the mortality and epidemiological pattern of dementia and its various major subtypes among urban and rural senior residents in Beijing.Methods Based on Beijing＇s dementia prevalence survey among residents aged 55 years and above in 1997,respondents were selected by stratified multiple‐stage cluster sampling and 12 urban communities and 17 rural village communities were randomly sampled then follow‐up in 2001.COX regression was used to analyze relative risks controllingconfounding factors on deaths of dementia cases.Results The mortality of dement patients in the 55‐64 age‐group was 0.82/100 person‐year.The age‐standardized mortality of dement cases was 0.90/100 person‐year.The mortality in the 65 and above age‐group was 1.44/100 person‐year,and the age‐standardized mortality was 1.56/100 person‐year.Among AD cases,the above two mortalities were 0.35/100 and 0.42/100 person‐year respectively,and among VaD cases,0.34/100 and 0.36/100 person‐year respectively.For both AD and VaD cases,their mortality increased with age.Region,gender and age were more significant to survival of AD cases.Conclusion One major subtype of dementia,AD,among elderly urban and rural residents in Beijing,has a different mortality and epidemiological pattern from VaD.

关键词： Dementia Mortality Alzheimer＇s disease Vascular dementia

Antimicrobial effect of sodium houttuyfonate on Staphylococcus epidermidis and Candida albicans biofilms

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Journal of Traditional Chinese Medicine 2013年第6期33卷 798-803页

作者： Jing Shao Huijuan Cheng Daqiang Wu Changzhong Wang Lingling Zhu zhenxin Sun Qiangjun Duan Weifeng huang Jinliang huang Laboratory of Microbiology and Immunology School of Chinese and Western Integrative Medicine Anhui University of Chinese Medicine

OBJECTIVE: To study antimicrobial effect of Sodium houttuyfonate(SH) on Staphylococcus epidermidis(SE) and Candida albicans(CA). METHODS: The prepared strain broths(OD600 =0.05) containing SE and CA were firstly used to test the minimal inhibitory concentrations(MICs) of SH, azithromycin(AZM) and fluconazole(FLU) by micro-dilution method. Then the biofilms of SE and CA were matured in 96-well plates, and co-cultured with SH, AZM and FLU for 1, 2 and 3 days to assess the antibiofilm efficacies of the agents with different concentrations by crystal violet staining method. At last, the treated biofilms of SE and CA by 2× MIC agents were observed by scanning electronic microscope. RESULTS: The MICs of SE and CA were 256 and 1024 μg/mL, respectively. After the 1st, 2nd and 3rd day of medications, the suppressions of biofilm were about 60%(P<0.01), 76%(P=0.000) and 75%(P=0.000) by 2×MIC SH, the suppressions of biofilm were about 90%(P=0.000), 88%(P=0.000) and 90%(P=0.000) by 2×MIC SH, which could be testified by scanning electron microscope results. However, the inhibitions of biofilm attachment had no significant difference for SE by SH and azithromycin and CA by SH and fluconazole. CONCLUSION: SH had widely anti-pathogenic effect on pathogenic biofilm formation of either bacteria or fungus, had more influence on enclosed cells of SE and CA than the traditional antibiotics, revealing its target might be the extracellular polymeric substances, and was more active to inhibit the growth of CA than SE.

关键词：表皮葡萄球菌白色念珠菌鱼腥草素钠抗菌效果生物膜扫描电子显微镜最小抑菌浓度电子显微镜观察