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Cyanobacteria-/cyanotoxin-contaminations and eutrophication status before Wuxi Drinking Water Crisis in Lake Taihu, China

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Journal of Environmental Sciences 2011年第4期23卷 575-581页

作者： Yongmei Liu Wei Chen Dunhai Li zebo huang Yinwu Shen Yongding Liu College of Pharmacy Wuhan University Wuhan 430071 China. Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China

After the appalling ＂Wuxi Drinking Water Crisis＂, increasing investigations concerning the contaminations of cyanobacterial blooms and their toxins in Lake Taihu have been performed and reported in the last two years. However, information regarding these issues before the crisis in 2007 remained insufficient. To provide some background data for further comparisons, the present study reported our investigations conducted in 2004, associated with the cyanotoxin contaminations as well as the eutrophication status in Lake Taihu. Results from the one-year-study near a drinking water resource for Wuxi City indicated that, unlike the status in recent two years, cyanobacteria and chlorophyta are the co-dominance species throughout the year. The highest toxin concentration （34.2 ng/mL） in water columns occurred in August. In bloom biomass, the peak value of intracellular toxin （0.59 μg/mg DW） was determined in October, which was lag behind that in water column. In addition, MC-RR was the major toxin variant throughout the year. During the study period, nutrients levels of total nitrogen and phosphorus were also recorded monthly. Results from the present study will lead to a better understanding of the eutrophication status and the potential risks before ＂Wuxi Drinking Water Crisis＂.

关键词： cyanobacterial blooms eutrophication microcystins Lake Taihu

Cementing mechanism of algal crusts from desert area

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Chinese Science Bulletin 2002年第16期47卷 1361-1368页

作者： HU Chunxiang LIU Yongding ZHANG Delu huang zebo B.S.Paulsen College of Life Sciences Northwest Normal UniversityLanzhou 730070China Institute of Hydrobiology Chinese Academy of SciencesWuhan 430072China School of Pharmacy University of Oslo1068 BlindemN-0316 OsloNorway

34-, 17-, 4-, 1.5-year old natural algal crusts were collected from Shapotou Scientific Station of the Chinese Academy of Sciences, 40-day old field and greenhouse artificial algal crusts were in situ developed in the same sandy soil and the same place (37°27’N, 104°57’E). Their different cohesions both against wind force and pressure were measured respectively by a sandy wind-tunnel experiment and a penetrometer. On the basis of these algal crusts, the cementing mechanism was revealed from many subjects and different levels. The results showed that in the indoor artificial crusts with the weakest cohesion bunchy algal filaments were distributed in the surface of the crusts, produced few extracellular polymers (EPS), the binding capacity of the crusts just accomplished by mechanical bundle of algal filaments. For field crusts, most filaments grew toward the deeper layers of algal crusts, secreted much more EPS, and when organic matter content was more than 2.4 times of chlorophyll a, overmuch organic

关键词： algal crust cohesion cementing structure gluing sub-stance cementing mechanism EPS.

Application of Flow Cytometry in Examination of Immunophenotypes in Human Cells

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Agricultural Biotechnology 2022年第6期11卷 145-148页

作者： zebo huang Aoxiang FENG Yuhan SHI Haoxian WANG Guanghong WU College of Life Science South China Agricultural UniversityGuangzhou 510642China

[Objectives]This study was conducted to establish a method for detecting the immunophenotypes of venous blood CIK cells in healthy donors and patients with triple-negative breast cancer or lung cancer by flow cytometry,and to further analyze and discuss the proportion of cellular immunophenotypes such as CD3^(+),CD4^(+),CD8^(+),CD3^(+)CD8^(+),CD3^(+)CD56^(+)in CIK cells.[Methods]Human venous blood was drawn,then anticoagulated with heparin and isolated with lymphocyte isolation solution,and the relevant immunophenotypes were detected by flow cytometry after 21 d of culture.[Results]The expression of CD3^(+)CD8^(+)and CD3^(+)CD56^(+)in the venous blood CIK cells was significantly higher in healthy donors than that in triple-negative breast and lung cancer patients.[Conclusions]CD3^(+)CD8^(+)and CD3^(+)CD56^(+)CIK cells have high anti-tumor activity.

关键词： Flow cytometry Immunophenotype Cluster of differentiation CIK

MiR-503 regulates cisplatin resistance of human gastric cancer cell lines by targeting IGF1R and BCL2

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Chinese Medical Journal 2014年第12期127卷 2357-2362页

作者： Wang Tongshan Ge Gaoxia Ding Yin Zhou Xin huang zebo Zhu Wei Shu Yongqian Liu Ping Department of Oncology First Affiliated Hospital of Nanjing Medical University NanjingJiangsu 210029China Department of Clinical Laboratory First Affiliated Hospital of Nanjing Medical University NanjingJiangsu 210029China State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjingJiangsu 210093China Department of Oncology Jiangsu Province HospitalNanjingJiangsu 210029China

Background Studies have shown that the drug resistance of gastric cancer cells can be modulated by abnormal expression of microRNAs （miRNAs）.We investigated the role of miR-503 in the development of cisplatin resistance in human gastric cancer cell *** MiR-503 expression was measured by quantitative real-time *** （3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide） and clonogenic assays were used to examine changes in cell viability and the drug resistance phenotype of cancer cells associated with upregulation or downregulation of the miRNA.A dual-luciferase activity assay was used to verify target genes of ***,Western blotting analysis,and a flow cytometric apoptosis assay were used to elucidate the mechanism by which miR-503 modulates drug resistance in cancer *** MiR-503 was significantly downregulated in gastric cancer tissues and several gastric cancer cell ***,downregulation of miR-503 in the cisplatin （DDP）-resistant gastric cancer cell line SGC7901/DDP was concurrent with the upregulation of insulin-like growth factor-1 receptor （IGF1R） and B-cell lymphoma 2 （BCL2） expression compared with the parental SGC7901 cell *** in vitro drug sensitivity assay showed that overexpression of miR-503 sensitized SGC7901/DDP cells to *** luciferase activity of reporters driven by IGF1R and BCL2 3＇-untranslated regions in SGC7901/DDP cells suggested that IGF1R and BCL2 were both direct target genes of *** miR-503 expression in SGC7901/DDP cells reduced expression of the target proteins,inhibited proliferation,and sensitized the cells to DDP-induced *** Our findings suggest that hsa-miR-503 modulates cisplatin resistance of human gastric cancer cells at least in part by targeting IGF1R and BCL2.

关键词： miR-503 cisplatin resistance insulin-like growth factor-1 receptor B-cell lymphoma 2 gastric cancer

Polysaccharides from Angelica sinensis alleviate neuronal cell injury caused by oxidative stress

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Neural Regeneration Research 2014年第3期9卷 260-267页

作者： Tao Lei Haifeng Li Zhen Fang Junbin Lin Shanshan Wang Lingyun Xiao Fan Yang Xin Liu Junjian Zhang zebo huang Weijing Liao Department of Rehabilitation Medicine Zhongnan Hospital and Cerebral Vascular Diseases Research Center Zhongnan Hospital Wuhan University School of Pharmaceutical Sciences Wuhan University

Angelica sinensis has antioxidative and neuroprotective effects. In the present study, we aimed to determine the neuroprotective effect of polysaccharides isolated from Angelica sinensis. In a pre-liminary experiment, Angelica sinensis polysaccharides not only protected PC12 neuronal cells from H202-induced cytotoxicity, but also reduced apoptosis and intracellular reactive oxygen species levels, and increased the mitochondrial membrane potential induced by H202 treatment. In a rat model of local cerebral ischemia, we further demonstrated that Angelica sinensis poly-saccharides enhanced the antioxidant activity in cerebral cortical neurons, increased the number of microvessels, and improved blood flow after ischemia. Our findings highlight the protective role of polysaccharides isolated from Angelica sinensis against nerve cell injury and impairment caused by oxidative stress.

关键词： nerve regeneration cerebral ischemia Angelica sinensis polysaccharides antioxidation reactive oxygen species rnitochondrial membrane potential apoptosis rnicrovessels NSFC grant neural regeneration

The phylogenetic analysis of Dalbergia (Fabaceae: Papilionaceae) based on different DNA barcodes

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The phylogenetic analysis of Dalbergia (Fabaceae: Papilionac...

旋转运动SMA层合梁动力学建模与非线性自由振动分析

中国生物工程学会第十一届学术年会暨2017年全国生物技术大会

作者： Qiwei Li Jihong Wu Yesheng Wang Xiaoming Lian Feilong Wu Lin Zhou zebo huang Shuang Zhu Center for Bioresources and Drug Discovery and School of Biosciences and Biopharmaceutics Guangdong Province Key Laboratory for Biotechnology Drug Candidates Guangdong Pharmaceutical University

The genus Dalbergia contains approximately 250 species with many valuable trees being destroyed by targeted and illegal *** barcoding is a reliable method for the molecular identification of different species and resources *** the present study,the specimen discrimination ability of internal transcribed spacer (ITS),matK,rbcL and psbA-trnH barcoding were tested on Dalbergia sequences,downloaded from the National Center for Biotechnology Information (NCBI),and the combined barcoding ITS+matK+rbcL was used to identify unknown *** was found that ITS+matK+rbcL have good discrimination rates based on the analysis methods best match (BM) and best close match (BCM).These barcodes also have the best performance concerning barcode gap distribution,and are able to discriminate unknown specimens from ***,it was demonstrated that *** and *** are also relatively close to sister-species *** and *** within the phylogenetic Dalbergia *** the overall performance of these barcodes,we suggest that the ITS+matK+rbcL region is a suitable barcode for identifying Dalbergia species.

关键词： Dalbergia spp. DNA barcoding identification ITS matK psbA-trnH rbcL

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西安理工大学学报 2022年第1期38卷 32-40页

作者：王航穆安乐黄泽波西安理工大学机械与精密仪器工程学院陕西西安710048 西安铁路职业技术学院机电工程学院陕西西安710026

层合梁铺层中内嵌SMA丝时,可以有效改变层合梁旋转运动时的动态特性。为了研究旋转运动SMA层合梁的振动特性,使用Brinson一维SMA本构模型获得温度变化时的恢复应力与马氏体体积分数。使用坐标变换建立层合梁质点在惯性系下的位移。基于E... 详细信息

层合梁铺层中内嵌SMA丝时,可以有效改变层合梁旋转运动时的动态特性。为了研究旋转运动SMA层合梁的振动特性,使用Brinson一维SMA本构模型获得温度变化时的恢复应力与马氏体体积分数。使用坐标变换建立层合梁质点在惯性系下的位移。基于Euler-Bernoulli梁和Von Karman大变形理论,建立了位移场与应变之间的关系。使用Hamilton变分原理,获得大范围旋转运动层合梁的动力学方程,并用Ritz法获得内嵌SMA丝层合梁的非线性自由振动方程。旋转运动对SMA层合梁的自由振动有明显的影响。研究了同一转速下,不同SMA体积分数、初始应变对固有频率的影响,而且研究了石墨层纤维铺设角度对层合梁基频的影响。

关键词：形状记忆合金非线性自由振动旋转层合梁

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脓毒血症严重程度预测模型的建立与验证

中外医药研究 2025年第1期4卷 12-15页

作者：黄泽波刘爱芬黎敏秀曾凡乔吴传黠广西壮族自治区桂东人民医院重症医学科广西壮族自治区梧州543000

目的:构建脓毒血症严重程度预测模型并进行验证。方法:选取广西壮族自治区桂东人民医院2022年4月—2023年4月收治的脓毒血症患者248例。根据病情严重程度分为一般组和严重组,比较两组临床指标,建立随机森林模型,并分析各指标对患者病情... 详细信息

目的:构建脓毒血症严重程度预测模型并进行验证。方法:选取广西壮族自治区桂东人民医院2022年4月—2023年4月收治的脓毒血症患者248例。根据病情严重程度分为一般组和严重组,比较两组临床指标,建立随机森林模型,并分析各指标对患者病情严重程度的影响,采用受试者工作特征(ROC)曲线下面积(AUC)分析随机森林预测模型对脓毒血症患者器官损害的预测价值,并进行验证。结果:248例患者中,严重脓毒血症113例,一般脓毒血症135例。严重组肌酐(SCr)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、总胆红素(TB)、降钙素原(PCT)、C反应蛋白(CRP)水平及急性生理学和慢性健康状况评价Ⅱ(APACHEⅡ)评分、序贯器官衰竭评估(SOFA)评分高于一般组(P<0.05)。以上述指标为变量建立随机森林模型,结果显示,APACHEⅡ评分影响最大。ROC曲线显示,训练集的AUC为0.972,验证集的AUC为0.959,随机森林预测模型校准曲线趋近于理想曲线。结论:SCr、AST、ALT、TB、PCT、CRP、APACHEⅡ评分、SOFA评分是影响脓毒血症患者病情严重程度的因素,据此建立的随机森林预测模型预测效能良好,可为临床评估病情提供参考。

关键词：脓毒血症严重程度预测模型随机森林

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动力锂电池放电特性实验研究与分析

电源世界 2016年第10期 30-32页

作者：黄泽波西南科技大学制造科学与工程学院四川绵阳621010

针对某动力锂电池进行一系列的放电实验,分析该锂电池在不同放电倍率下的特性,为测试提供了测试参数,讨论了该锂电池容量的测试依据,并测试了某品牌10Ah锂电池放电特性及容量,为锂电池的快速检测提供测试依据。

关键词：动力锂电池放电特性锂电池检测放电倍率