检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

肿瘤类器官及其在合成生物学中的研究进展

合成生物学 2024年第1期5卷 191-201页

作者：孟倩尹聪黄卫人深圳大学第一附属医院泌尿外科国家地方联合医学合成生物学临床应用关键技术工程实验室广东深圳518036 中国科学院深圳先进技术研究院合成生物学研究所广东深圳518000

类器官技术的发展为更接近机体细胞组成和病理生理特征的癌症模型开辟了新途径。患者来源的肿瘤类器官在多次传代后仍能维持原有肿瘤的组织病理学及遗传表型特征,不仅可作为测试新型抗癌药物的优良模型,也可通过其药物敏感性测试预测患... 详细信息

类器官技术的发展为更接近机体细胞组成和病理生理特征的癌症模型开辟了新途径。患者来源的肿瘤类器官在多次传代后仍能维持原有肿瘤的组织病理学及遗传表型特征,不仅可作为测试新型抗癌药物的优良模型,也可通过其药物敏感性测试预测患者的临床反应,为肿瘤患者的个体化精准治疗提供可靠的依据。合成生物学是以工程学思想为指导,提供独特工具来重建空间和动态信号,调控细胞间通信。合成生物学的快速发展,为肿瘤类器官在肿瘤的发生发展及肿瘤治疗等方面提供了一系列崭新的思路和方法,包括如何工程化重建类器官空间与动态信号、细胞稳态维持、细胞间通信调控等。本文概述了肿瘤类器官的构建过程及其在合成生物学中的应用,讨论了肿瘤类器官当前在构建效率、标准化、自动化、精确度等方面的局限性,最后展望了合成生物学在推动肿瘤类器官结构和功能复杂化方面的前景。

关键词：肿瘤类器官药物筛选精准医疗基因线路合成生物学

基于ERK1/2翻译后修饰调控及空间性调节的抗癌策略研究进展

在线全文

学校读者我要写书评

暂无评论

肿瘤防治研究 2024年第6期51卷 475-483页

作者：虢婷尚梦宇郭茵黄卫人广西中医药大学研究生院南宁530200 深圳大学医学部基础医学院深圳518061 汕头大学医学院药理学教研室汕头515041 深圳市第二人民医院转化医学研究院深圳518025

ERK1/2是一种介导细胞信号转导的关键蛋白,参与调节细胞的染色质重塑、核解体、增殖、存活、代谢、迁移和分化等生物学过程。其过度激活与癌症的发生进展密切相关,机制表现为上游通路分子或调节因子的基因突变使ERK1/2过度激活及针对上... 详细信息

ERK1/2是一种介导细胞信号转导的关键蛋白,参与调节细胞的染色质重塑、核解体、增殖、存活、代谢、迁移和分化等生物学过程。其过度激活与癌症的发生进展密切相关,机制表现为上游通路分子或调节因子的基因突变使ERK1/2过度激活及针对上述靶点进行抑制后的ERK1/2再激活。因而ERK1/2为一个有潜在价值的靶点。本文对ERK1/2的翻译后修饰调控及空间性调节的机制研究和相应小分子抑制剂的应用现状进行了讨论,总结并展望了目前靶向调控ERK1/2的抗癌策略及针对潜在靶点展开探索的可能性,为ERK1/2作为抗癌理想靶点的开发性研究提供了新思路。

关键词： ERK1/2 肿瘤靶向治疗翻译后修饰二聚化小分子抑制剂

维普期刊数据库博看期刊

Strategy for the mapping of interactive genes using bulked segregant analysis method and Mapmaker/Exp software

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2006年第21期51卷 2619-2623页

作者： WU weiren huang Biguang College of Crop Science Fujian Agriculture and Forestry UniversityFuzhou350002 China

A qualitative trait is usually controlled by a single gene, but it may be sometimes controlled by two or even more genes. This phenomenon is called gene interaction. Rapidly searching for linked mo- lecular markers via bulked segregant analysis (BSA) and then constructing regional linkage map with Mapmaker/Exp has become a common approach to mapping single major genes. However, methods and computer programs developed for mapping single major genes cannot be simply applied to interactive genes because the genetic patterns of gene interac- tions are quite different from that of single-gene in- heritance. Up to now, experimental methods for quickly screening molecular markers linked to inter- active genes and statistical methods and corre- sponding computer softwares for simultaneously analyzing the linkage relationships of multiple mo- lecular markers to an interactive gene have not been available. To solve this problem, in this paper, we propose a strategy for mapping interactive genes using BSA and Mapmaker/Exp. We demonstrate that all interactive genes can be mapped by the 'BSA + Mapmaker/Exp' strategy using F2 generation (in a few cases, F3 generation is also needed). As BSA and Mapmaker/Exp have been broadly used in gene mapping studies and are well known by many re- searchers, the strategies proposed in this paper will be useful for practical researches.

关键词：基因相互作用基因作图制图软件安全

Recent development on synthetic biological devices treating bladder cancer

在线全文

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2016年第4期1卷 216-220页

作者： Zhicong Chen Anbang He Yuchen Liu weiren huang Zhiming Cai Key Laboratory of Medical Reprogramming Technology Shenzhen Second People's HospitalThe First Affiliated Hospital of Shenzhen UniversityShenzhen518039 Guangdong ProvincePeople's Republic of China Department of Urology Peking University First HospitalInstitute of UrologyPeking UniversityNational Urological Cancer CentreBeijing100034China

Synthetic biology is an emerging field focusing on engineering genetic devices and biomolecular systems for a variety of applications from basic biology to biotechnology and *** to the tremendous advances in genomics and the chemical synthesis of DNA in the past decade,scientists are now able to engineer genetic devices and circuits for cancer research and intervention,which offer promising therapeutic strategies for cancer *** this article,we provide a systemic review on recent development achieved by the synthetic biologists,oncologists and clinicians of one National“973”*** expand the synthetic biology toolkits involving DNA,RNA and protein bio-parts to explore various issues in cancer research,such as elucidation of mechanisms and pathways,creation of new diagnostic tools and invention of novel therapeutic *** claimed that the Chinese synthetic biologists are promoting the basic research productions of tumor synthetic biology into the clinic.

关键词： Synthetic biology Bladder cancer Biomarker Gene therapy

Comparative studies on histological and ultra-structure of the pituitary of different ploidy level fishes

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2006年第5期49卷 446-453页

作者： LONG Yu LIU Shaojun huang weiren ZHANG Jian SUN Yuandong ZHANG Chun CHEN Song LIU Jinghui LIU Yun College of Life Sciences Hunan Normal UniversityChangsha 410081China

The histological and ultra-structure of the pituitary in diploid red crucian carp(Carassius auratus red var.),triploid crucian carp and allotetraploid hybrids within and after the breeding season were comparatively *** result showed that there were six endocrine cell types in the pituitary of these three kinds of fishes,and there was an obvious difference in cell size among different ploidy level *** for the same type of pituitary cells,the cell size was increased gradually with the in-creasing ploidy *** the breeding season,the allotetraploid hybrids had higher proportion of go-nadotropin cells(GTH)than triploids,and the triploids had higher proportion of GTH than *** results were related to the earlier sexual maturity of allotetraploid hybrids and sterility of triploid cru-cian *** the other hand,among the three kinds of fishes,the proportion of somatotropin(STH)cells in triploids crucian carp was the highest,whereas that in allotetraploid hybrids was the *** results might be connected with the faster growth rate of triploids and slower growth rate of al-lotetraploid *** addition,in GTH cells of meso-adenohypophysis after the breeding season,there were many endocrine particles in triploids,while those endocrine particles were released from the cells in allotetraploids and *** result showed that the sterility of triploid crucian carp might be related to the hormone which was not released from the GTH *** a word,the present study indicated that the differences in the structure of pituitary among different ploidy level fishes contributed to their difference in the growth rate and gonadal development.

关键词： diploid triploid ailotetraploid pituitary histological structure ultra-structure

Directing cellular information flow via CRISPR signal Conductors

在线全文

学校读者我要写书评

暂无评论

Directing cellular information flow via CRISPR signal Conduc...

The roles of long non-coding RNAs in renal cell carcinomas development

中国生物化学与分子生物学会2016年全国学术会议

作者： Yuchen Liu weiren huang State Engineering Laboratory of Medical Key Technologies Application of Synthetic Biology Shenzhen Second People’s Hospital

The complex phenotypes of eukaryotic cells are controlled bydecision-making circuits and signaling pathways.A key obstacleto implementing artificial connections in signaling networkshas been the lack of synthetic devices for efficient sensing,processing and controlof biological *** extendingsg RNAs to include modified riboswitches that recognize

关键词： CRISPR signal logic gate fatedifferentiation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The roles of long non-coding RNAs in renal cell carcinomas d...

Multi-environment BSA-seq using large F3 populations is able to achieve reliable QTL mapping with high power and resolution: An experimental demonstration in rice

中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议

作者： Xiaoqiang Guo Ming Xia weiren huang Zhiming Cai Shenzhen Key Laboratory of Genitourinary Tumor Shenzhen Second People's Hospital First Affiliated Hospital of Shenzhen University

Long non-coding RNAs(long ncRNAs, lncRNA) are non-protein coding transcripts longerthan 200 nucleotides. lncRNAs play important roles in a wide range of biological processessuch as the regulation of gene transcription, post-transcriptional regulation,and epigeneticregulation, and dysregulated lncRNAs are involved in cancer. In this study, the expressionsof several lncRNAs including homeobox(HOX) transcript antisense intergenic RNA(HOTAIR), metastasis-associated lung adenocarcinoma transcript 1(Malat-1) and lincRNA-p21 were measured with quantitative real-time polymerase chain reaction(qRT-PCR)in cancer tissues and adjacent normal tissues of 48 paired renal cell carcinomas(RCC). Theresults indicated the RNA levels of three lncRNAs HOTAIR, Malat-1 and lincRNA-p21 aredysregulated, and the contents of three lncRNAs are associated with partly clinical data. It demonstrated that lncRNAs abnormality is an important reason for development of RCC,and the mechanism is required to be further investigated.

关键词： lncRNA renal cell carcinomas HOTAIR Malat-1 lincRNA-p21

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The Crop Journal 2024年第2期12卷 549-557页

作者： Yan Zheng Ei Ei Khine Khin Mar Thi Ei Ei Nyein Likun huang Lihui Lin Xiaofang Xie Min Htay Wai Lin Khin Than Oo Myat Myat Moe San San Aye weiren Wu College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Fujian Provincial Key Laboratory of Crop Breeding by Design Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Department of Botany Mawlamyine UniversityMon StateMawlamyineMyanmar Department of Botany Dagon UniversityNorth DagonYangonMyanmar

Bulked-segregant analysis by deep sequencing(BSA-seq) is a widely used method for mapping QTL(quantitative trait loci) due to its simplicity, speed, cost-effectiveness, and efficiency. However, the ability of BSA-seq to detect QTL is often limited by inappropriate experimental designs, as evidenced by numerous practical studies. Most BSA-seq studies have utilized small to medium-sized populations, with F2populations being the most common choice. Nevertheless, theoretical studies have shown that using a large population with an appropriate pool size can significantly enhance the power and resolution of QTL detection in BSA-seq, with F_(3)populations offering notable advantages over F2populations. To provide an experimental demonstration, we tested the power of BSA-seq to identify QTL controlling days from sowing to heading(DTH) in a 7200-plant rice F_(3)population in two environments, with a pool size of approximately 500. Each experiment identified 34 QTL, an order of magnitude greater than reported in most BSA-seq experiments, of which 23 were detected in both experiments, with 17 of these located near41 previously reported QTL and eight cloned genes known to control DTH in rice. These results indicate that QTL mapping by BSA-seq in large F_(3)populations and multi-environment experiments can achieve high power, resolution, and reliability.

关键词： BSA-seq QTL mapping Large F3 population Multi-environment experiment Cross-validation

流动人口对地方政府基础教育公共财政支出的影响研究——基于2010-2014年福建省58个县市数据分析

在线全文

学校读者我要写书评

暂无评论

教育学术月刊 2016年第6期 46-52页

作者：段哲哲黄伟任台湾政治大学公共行政系泉州农商银行

对福建省58个县市2010-2014年的面板数据进行回归分析发现,地方教育财政支出会受到流动人口、地方经济发展、转移支付等因素影响;人口流动会对人口流入地县市产生教育资源挤占效应,地方政府基础教育财政支出存在自利性动机,为了实现本... 详细信息

对福建省58个县市2010-2014年的面板数据进行回归分析发现,地方教育财政支出会受到流动人口、地方经济发展、转移支付等因素影响;人口流动会对人口流入地县市产生教育资源挤占效应,地方政府基础教育财政支出存在自利性动机,为了实现本地利益最大化,会对流动人口子女在本地入学设置相关门槛。这表明,在发展地方经济、加大转移支付的同时,需要对源户籍制度进行改革,对附着于户籍的福利待遇作彻底的剥离,接纳农民工子女进入本地公立学校,以实现中央"两为主"之政策目标。

关键词：人口流动基础教育财政支出户籍