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Evolution of strontium isotopic composition of sea-water from Late Permian to Early Triassic based on study of marine carbonates,Zhongliang Mountain,Chongqing,China

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Science China Earth Sciences 2008年第4期51卷 528-539页

作者： huang SiJing Qing HaiRuo huang peipei HU ZuoWei WANG QingDong ZOU MingLiang LIU HaoNian State Key Laboratory of Oil/Gas Reservoir Geology and Exploitation Institute of Sedimentary GeologyChengdu University of TechnologyChengdu 610059China Department of Geology University of ReginaRegina S4S 0A2Canada

Collected from a Late Permian to Early Triassic sedimentary section in the Zhongliang Mountain of Chongqing, Southwest China, sixty marine carbonate samples were measured for the 87Sr/86Sr ratios, and corresponding evolution curve was constructed. The concentrations of SiO2, CaO, MgO, Mn and Sr are used to evaluate reservation of strontium isotopic composition for original seawater and the credi-bility of the dissolution method for sample preparation. The results show that most of the samples (except seven samples with the Mn/Sr ratios higher than 2) contain the original geochemistry signa-tures of ancient seawater. Compared to the published 87Sr/86Sr ratios from the Late Permian to Early Triassic, our database reported here is the largest and the curve constructed is the most complete. The strontium isotopic curve from Late Permian to Early Triassic is consistent globally and exhibits a gen-eral trend of steady increase during this period. The minimum of 87Sr/86Sr ratios (0.707011) occurs in the Late Permian (30 m in thickness below the Permian-Triassic boundary), and the maximum (0.708281), near the Early-Middle Triassic boundary. The lack of land plants and the rapid continental weathering result in the increase of 87Sr/86Sr ratios during the interval. The Permian-Triassic boundary in Zhongli-ang Mountain Section has been accepted internationally. The 87Sr/86Sr ratios of six samples near the boundary vary from 0.70714 to 0.70715 with an average of 0.70714, which is consistent with the value of 0.70715 (samples are from articulate brachiopod shells) from Korte et al. published in 2006 (within the error range in experiment). Accordingly, the strontium isotope composition in the Permian-Triassic boundary in this paper is of global significance. It can be confirmed that the 87Sr/86Sr ratios of the sea-water in the Permian-Triassic transition are in the range of 0.70714―0.70715.

关键词： Zhongliang Mountain Chongqing Late Permian-Early Triassic paleoseawater strontium isotope composition

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Carbonaceous aerogel and CoNiAl-LDH@CA nanocomposites derived from biomass for high performance pseudo-supercapacitor

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Science Bulletin 2017年第12期62卷 841-845页

作者： Sidi Zhang Jian Liu peipei huang Hao Wang Changyan Cao Weiguo Song Beijing National Laboratory for Molecular Sciences Key Laboratory of Molecular Nanostructure and NanotechnologyCAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China The Academy of Military Economics Student Brigade Wuhan 430035China

Conversion of waste biomass to valuable carbonaceous material is a sustainable and environmental benign method for energy and reduction of greenhouse gas emission. Herein, a two-step hydrothermal method was developed to fabricate high performance electrode material from pomelo peels. In the first step, the pomelo peels were transformed to carbonaceous aerogel （CA）, which constructed of three- dimensional, sponge-like brown monolith with hierarchical pores, low-density （0.032 g]cm3） and excel- lent mechanical flexibility. Then, the cobalt nickel aluminum layered double hydroxide （CoNiAI-LDH） was in situ loaded on the surface of CA to form exquisite core-shell structure （CoNiAI-LDH@CA） through the second hydrothermal step. When used as an electrode material for supercapacitor, CoNiA1-LDHOCA exhibited high specific capacitances of 1,134F/g at 1A/g and 902Fig at 10A/g, respectively. Furthermore, they displayed an excellent cycling stability without an obvious capacitance decrease after 4,000 cycles.

关键词： BiomassCarbonaceous aerogelLayered double hydroxideSupercapacitor

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Loss of stat3 function leads to spine malformation and immune disorder in zebrafish

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Science Bulletin 2017年第3期62卷 185-196页

作者： Shuting Xiong Junjie Wu Jing Jing peipei huang Zhi Li Jie Mei Jian-Fang Gui College of Fisheries Key Laboratory of Freshwater Animal BreedingMinistry of AgricultureFreshwater Aquaculture Collaborative Innovation Center of Hubei ProvinceHuazhong Agricultural University State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesUniversity of Chinese Academy of Sciences

STAT （Signal Transducers and Activators of Transcription） gene family members have been revealed to be involved in cell growth and differentiation in vertebrates. Despite their physiological importance, their functions are poorly studied at organ and systemic levels. In this study, we performed a genome-wide analysis using data from invertebrates to vertebrates to identify STAT genes and analyze their evolutionary history. Interestingly, the STAT gene family undergoes genome duplications during the evolutionary history with STAT3 homologues firstly appearing in the basal extant vertebrate, sea lamprey, suggesting its possible roles in spine formation. To investigate the functions of stat3 in fish species, TALEN technology was performed to generate mutant zebrafish lines, star3 mutant zebrafish showed no obvious defects at early developmental stage but displayed severe lateral and vertical curvature of the spine （scoliosis）, spine fracture and the incomplete bone joints with narrower junction between vertebrae at early juvenile stage, as indicated by Alizarin red and Alcian blue staining, radiography and micro-computed tomography （MicroCT） analysis. Transcriptome analysis reveals dramatic alterations in a number of genes involved in immune and infection response, skeletal development and somatic growth, especially downregulated expression of collagen gene family, in the juvenile stat3 mutant zebrafish. Moreover, most of the collagen genes were detected to have abnormal expression pattern during the formation of spine deformities in stat3 mutants. Our data reveal that stat3 is specially expressed in vertebrates and required for normal spine development and immune function in zebrafish.

关键词： Star3 Zebrafish Spine malformation Immune disorder

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Improving the electrochemical performance of Fe3O4 nanoparticles via a double protection strategy through carbon nanotube decoration and graphene networks

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Nano Research 2015年第4期8卷 1339-1347页

作者： Shuliang Yang Changyan Cao Gen Li Yongbin Sun peipei huang Fangfang Wei Weiguo Song Beijing National Laboratory for Molecular Sciences Laboratory of Molecular Nanostructures and Nanotechnology Institute ofChemistry Chinese Academy of Sciences Beijing 100190 China

Iron oxide is a promising anode material for lithium ion batteries, but it usually exhibits poor electrochemical property because of its poor conductivity and large volume variation during the lithium uptake and release processes. In this work, a double protection strategy for improving electrochemical performance of Fe3O4 nanoparticles through the use of decoration with multi-walled carbon nanotubes and reduced graphene oxides networks has been developed. The resulting MWCNTs-Fe3O4-rGO nanocomposites exhibited excellent cycling performance and rate capability in comparison with MWCNTs-Fe3O4, MWCNTs-Fe3O4 physically mixed with rGO, and Fe3O4-rGO. A reversible capacity of -680 mA·h·g^-1 can be maintained after 100 cycles under a current density of 200 mA.g^-1.

关键词： Fe3O4 carbon nanotube graphene lithium ion batteries anode materials

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Essential roles of stat5.1/stat5b in controlling fish somatic growth

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Journal of Genetics and Genomics 2017年第12期44卷 577-585页

作者： Shuting Xiong Jie Mei peipei huang Jing Jing Zhi Li Jingliang Kang Jian-Fang Gui College of Fisheries Key Laboratory of Freshwater Animal Breeding Ministry of Agriculture Huazhong Agricultural University State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences University of the Chinese Academy of Sciences Medical Research Institute Wuhan University

Signal transducer and activator of transcription 5b（STAT5b） has been identified as a key downstream mediator of growth hormone（GH） signaling in somatic growth of mammalian. However, the corresponding homologue gene of Stat5b is unknown in fish species. In this study, we generated loss-offunction mutants in stat5.1 and stat5.2, two stat5 homologues existing in zebrafish. In stat5.1-deficient zebrafish, a significant reduction of body length and body weight was detected in the embryos/larvae and adults compared with the wild-type control fish, and sexual size dimorphism in adult zebrafish was also eliminated. However, the stat5.2-deficient zebrafish displayed a normal developmental phenotype during all lifespan. Chromatin immunoprecipitation combined with deep sequencing（Ch IP-seq） method was adopted to further investigate the potential transcriptional targets of Stat5 protein and cast much light upon the biological function of Stat5. We identified more than 800 genes as transcriptional targets of Stat5 during zebrafish embryogenesis. KEGG analysis indicated that the Stat5 target gene network is predominantly linked to the metabolic pathways, neuroactive ligand-receptor interaction and JAK-STAT signaling pathways. Further validation studies suggested that Stat5.1 protein could directly regulate the expression of gh1, and stat5.1-mutated zebrafish showed a reduction of gh1 mRNA level. In the present study, stat5.1 was revealed as the corresponding homologue gene of Stat5b in fish species. Additionally,we found a novel molecular interaction between Stat5.1/Stat5b and GH, and unraveled a positive feedback loop Stat5.1-GH-Stat5.1 which is necessary for somatic growth and body development in zebrafish.

关键词： Zebrafish Stat5 Body growth Sexual size dimorphism Growth hormone

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Anion exchange membranes with a semi-interpenetrating polymer network using 1,6-dibromohexane as bifunctional crosslinker

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Chinese Journal of Chemical Engineering 2024年第8期72卷 199-208页

作者： Aijie Li Zhanliang Wang Zhihao Si Lu Lu peipei huang Jinhong Liu Songyuan Yao Peiyong Qin Xinmiao Zhang National Energy R&D Center for Biorefinery College of Life Science and TechnologyBeijing University of Chemical TechnologyBeijing 100029China Paris Curie Engineer School Beijing University of Chemical TechnologyBeijing 100029China Environmental Protection Research Institute Beijing Research Institute of Chemical IndustryBeijing 100000China

An anion exchange membrane(AEM)is generally expected to possess high ion exchange capacity(IEC),low water uptake(WU),and high mechanical strength when applied to electrodialysis *** different types of AEMs,semi-interpenetrating polymer networks(SIPNs)have been suggested for their structural superiorities,i.e.,the tunable local density of ion exchange groups for IEC and the restrained leaching of hygroscopic groups by insolubility for ***,the conventional SIPN AEMs still struggle to balances IEC,WU,and mechanical strength simultaneously,due to the lack of the compact crosslinking *** this work,we proposed a novel SIPN structure of polyvinylidene difluoride/polyvinylimidazole/1,6-dibromohexane(PVDF/PVIm/DBH).On the one hand,DBH with two cationic groups of imidazole groups are introduced to enhance the ion conductivity,which is different from the conventional monofunctional modifier with only one cationic *** the other hand,DBH has the ability to bridge with PVIm,where the mechanical strength of the resulting AEM is increased by the increase of crosslinking *** show that a low WU of 38.1%to 62.6%,high IEC of 2.12—2.22 mmol·g^(-1),and excellent tensile strength of 3.54—12.35 MPa for PVDF/PVIm/DBH membrane are *** work opens a new avenue for achieving the high-quality AEMs.

关键词： Anion exchange membrane Polyvinylidene difluoride Electrodialysis Semi-interpenetrating polymer networks

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A novel PDZ domain-containing gene is essential for male sex differentiation and maintenance in yellow catfish(Pelteobagrus fulvidraco)

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Science Bulletin 2018年第21期63卷 1420-1430页

作者： Cheng Dan Qiaohong Lin Gaorui Gong Tianyi Yang Shuting Xiong Yang Xiong peipei huang Jian-Fang Gui Jie Mei College of Fisheries Huazhong Agricultural University State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences University of Chinese Academy of Sciences

The sex-determining genes are found to be variable among different fish species. Yellow catfish(Pelteobagrus fulvidraco) is an important aquaculture fish species in China with XX/XY sex-determining type. Recently, YY super-male yellow catfish has been successfully produced by combining hormonalinduced sex reversal method with sex chromosome-linked markers. Here, we identified a novel PDZ domain-containing gene in yellow catfish designated as pfpdz1, in whose intron the sex-linked marker was located. The coding sequence of pfpdz1 in Y chromosome was identical to that in X chromosome except a missense SNP(A/T) that changes an amino acid(E8V) in the N-terminal region. Pfpdz1 displayed male-specific expression during sex differentiation. Overexpression of pfpdz1 using additive transgenesis induces XX ovary to differentiate into testis-like tissue, while the targeted inactivation of pfpdz1 in Y chromosome using CRISPR/Cas9-mediated mutagenesis triggers ovarian differentiation. Furthermore,we demonstrated that pfpdz1 initiates testicular differentiation through upregulating expression of amh, dmrt1 and sox9a1, as well as downregulating expression of cyp19a1, foxl2 and wnt4. Our data provide functional evidence that pfpdz1 is significant for male differentiation and maintenance in yellow catfish.

关键词： Sex differentiation Yellow catfish Pfpdz1 Sex reversal

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学前教育与经济发展的非线性关系研究——基于学前教育财政投入的门限效应分析

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调研世界 2023年第1期 80-88页

作者：黄佩佩中国财政科学研究院

本文采用我国31个省(区、市)2007—2020年的年度面板数据,以人类发展指数(HDI)(1)框架下编制的中国学前教育发展指数作为核心解释变量,以学前教育财政投入占地区生产总值(GDP)比重作为门限变量,探讨学前教育与经济发展之间的非线性关系... 详细信息

本文采用我国31个省(区、市)2007—2020年的年度面板数据,以人类发展指数(HDI)(1)框架下编制的中国学前教育发展指数作为核心解释变量,以学前教育财政投入占地区生产总值(GDP)比重作为门限变量,探讨学前教育与经济发展之间的非线性关系。研究结果表明:(1)从总体趋势看,学前教育促进了经济发展并存在双重门限效应,当学前教育财政投入占GDP比重在0.34%~0.42%之间时,学前教育对经济发展的促进作用最大。(2)从控制变量看,金融发展仅对东部地区经济发展存在显著正向影响,中部地区、西部地区经济发展更多地依赖城镇化水平的提高和产业结构优化,政府支出仅对东北地区经济发展存在正向影响但不显著。(3)从区域异质性看,东部地区和东北地区学前教育对经济发展存在学前教育财政投入单门限效应;中部地区、西部地区不存在门限效应,学前教育质量有待提高。

关键词：学前教育财政投入经济发展门限效应

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Nanocarbon-based TEMPO as stable heterogeneous catalysts for partial oxidation of alcohols

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Science Bulletin 2016年第10期61卷 772-777页

作者： Yongbin Sun Changyan Cao Fang Wei peipei huang Shuliang Yang Weiguo Song CAS Key Laboratory of Molecular Nanostructures and Nanotechnology Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China

Polymerized fullerene hollow spheres bonded with 2,2,6,6-tetramethylpiperidine-l-oxyl （TEMPO） have been successfully synthesized via amination of C6o with 4-amino-TEMPO in the presence of H202, and then cross- linked by 1,6-hexanediamine. The hollow spheres were analyzed by fourier transform infrared spectrometer, electron spin resonance and X-ray photoelectron spec- troscopy analysis, which indicated the presence of N-O free radicals in the products. When used as a typical heterogeneous catalyst for selective oxidation of alcohols to the corresponding aldehydes or ketones, it exhibited excellent activities, selectivity and recyclability. This synthesis route is convenient and effective, and may provide a new approach to developing immobilized full- erene based heterogeneous catalysts with high activity and recyclability.

关键词： Fullerene . Hollow sphere TEMPO Heterogeneous catalyst Oxidation of alcohols

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Origin and chromatin remodeling of young X/Y sex chromosomes in catfish with sexual plasticity

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National Science Review 2023年第2期10卷 91-106页

作者： Gaorui Gong Yang Xiong Shijun Xiao Xi-Yin Li peipei huang Qian Liao Qingqing Han Qiaohong Lin Cheng Dan Li Zhou Fan Ren Qi Zhou Jian-Fang Gui Jie Mei Hubei Hongshan Laboratory College of Fisheries Huazhong Agricultural University Jiaxing Key Laboratory for New Germplasm Breeding of Economic Mycology State Key Laboratory of Freshwater Ecology and Biotechnology Hubei Hongshan Laboratory Institute of HydrobiologyChinese Academy of Sciences University of the Chinese Academy of Sciences School of Animal Science and Nutritional Engineering Wuhan Polytechnic University MOE Laboratory of Biosystems Homeostasis & Protection Life Sciences InstituteZhejiang University

Assembly of a complete Y chromosome is a significant challenge in animals with an XX/XY sex-determination *** cently,we created YY-supermale yellow catfish by crossing XY males with sex-reversed XY females,providing a valuable model for Y-chromosome assembly and ***,we assembled highly homomorphic Y and X chromosomes by sequencing genomes of the YY supermale and XX female in yellow catfish,reve aling their nucleotide divergences with only less than 1% and with the same gene *** sex-determining region(SDR) was identified to locate within a physical distance of0.3 Mb by ***,the incipient sex chromosomes were revealed to originate via autosome-autosome fusion and were characterized by a highly rearranged region with an SDR downstream of the fusion *** found that the Y chromosome was at a very early stage of differentiation,as no clear evidence of evolutionary strata and classical structure features of recombination suppression for a rather late stage of Y-chromosome evolution were ***,a number of sex-antagonistic mutations and the accumulation of repetitive elements were discovered in the SDR,which might be the main driver of the initial establishment of recombination suppression between young X and Y ***,distinct three-dimensional chromatin organizations of the Y and X chromosomes were identified in the YY supermales and XX females,as the X chromosome exhibited denser chromatin structure than the Y chromosome,while they respectively have significantly spatial interactions with female-and male-related genes compared with other *** chromatin configuration of the sex chromosomes as well as the nucleus spatial organization of the XX neomale were remodeled after sex reversal and similar to those in YY supermales,and a male-specific loop containing the SDR was found in the open chromatin *** results elucidate the origin of young sex chromosomes and the chromatin remodel

关键词： Y chromosome sex-chromosome evolution chromosome fusion chromatin organization sexual reversal

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