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Etiological roles of core promoter variation in triple-negative breast cancer

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Genes & Diseases 2023年第1期10卷 228-238页

作者： Teng huang Jiaheng Li San ming Wang Cancer Centre and Institute of Translational Medicine Faculty of Health SciencesMinistry of Education Frontiers Science Center for Precision OncologyUniversity of MacaoTaipaMacao SAR 999078China

Abnormal geneexpression playskeyrole in cancerdevelopment.Acorepromoter is located around the transcriptional start site.Through interaction between core promoter sequences and transcriptional factors,core promoter controls transcriptional initiation.We hypothesized that in cancer,core promoter sequences could be mutated to interfere the interaction with transcriptional factors,resulting in altered transcriptional initiation and abnormal gene expression and cancer development.We used triple-negative breast cancer(TNBC)as a model to test our hypothesis.We collected genome-wide core promoter variants from 279 TNBC genomes.After extensive filtering of normal genomic polymorphism,we identified 19,427 recurrent somatic variants in 1,238 core promoters of 1,274 genes and 1,694 recurrent germline variants in 272 core promoters of 294 genes.Many of the affected genes were oncogenes and tumor suppressors.Analysis of RNA-seq data from the same patient cohort identified increased or decreased gene expression in 439 somatic and 85 germline variantsaffected genes,and the results were validated by luciferase reporter assay.By comparing with the core promoter variation data from 610 unclassified breast cancer,we observed that core promoter variants in TNBC were highly TNBC-specific.We further identified the drugs targeting the genes with core promoter variation.Our study demonstrates that core promoter is highly mutable in cancer,and can play etiological roles in TNBC and other types of cancer through influencing transcriptional initiation.

关键词： Core promoter RNA-seq Triple-negative breast cancer Variation Whole exome sequencing

Global Zero-relaxation Limit Problem of the Electro-diffusion Model Arising in Electro-Hydrodynamics

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Acta Mathematicae Applicatae Sinica 2024年第1期40卷 241-268页

作者： ming-hua Yang Si-ming huang Jin-yi Sun Department of Mathematics Jiangxi University of Finance and EconomicsNanchang330032China School of Public Administration Guangdong University of Foreign StudiesGuangzhou510006China College of Mathematics and Statistics Northwest Normal UniversityLanzhou730070China

In this paper,we study a global zero-relaxation limit problem of the electro-diffusion model arising in electro-hydrodynamics which is the coupled Planck-Nernst-Poisson and Navier-Stokes equations.That is,the paper deals with a singular limit problem of{u^(∈)_(t)+u^(∈)·▽u^(∈)-Δu^(∈)+▽P^(∈)=Δφ^(∈)▽φ^(∈),in R^(3)×(0,∞),▽·u^(∈)=0,in R^(3)×(0,∞),n^(∈)_(t)+u^(∈)·▽n^(∈)-Δn^(∈)=-▽·(n^(∈)▽φ^(∈)),in R^(3)×(0,∞),ct+u^(∈)·▽c^(∈)-Δc^(∈)=▽·(c^(∈)▽φ^(∈)),in R^(3)×(0,∞),∈^(-1)φ^(∈)_(t)=Δφ^(∈)-n^(∈)+c^(∈),in R^(3)×(0,∞),(u^(∈),n^(∈),c^(∈),φ^(∈))|t=0=(u0,n0,c0,φ0),in R^(3) involving with a positive,large parameter^(∈).The present work show a case that(u^(∈),n^(∈),c^(∈))stabilizes to(u^(∞),n∞,c∞):=(u,n,c)uniformly with respect to the time variable as^(∈)→+∞with respect to the strong topology in a certain Fourier-Herz space.

关键词： fourier-Herz space littlewood-Paley decomposition stability limit dissipative system

Kinesin 26B modulates M2 polarization of macrophage by activating cancer-associated fibroblasts to aggravate gastric cancer occurrence and metastasis

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World Journal of Gastroenterology 2024年第20期30卷 2689-2708页

作者： Lian-Meng huang ming-Jin Zhang Department of General Surgery The 901st Hospital of PLAHefei 230031Anhui ProvinceChina

BACKGROUND The regulatory effects of KIF26B on gastric cancer(GC)have been confirmed,but the specific mechanism still needs further exploration.Pan-cancer analysis shows that the KIF26B expression is highly related to immune infiltration of cancerassociated fibroblasts(CAFs),and CAFs promote macrophage M2 polarization and affect cancers’progression.AIM To investigate the regulatory functions of KIF26B on immune and metastasis of GC.METHODS We analyzed genes’mRNA levels by quantitative real-time polymerase chain reaction.Expression levels of target proteins were detected by immunohistochemistry,ELISA,and Western blotting.We injected AGS cells into nude mice for the establishment of a xenograft tumor model and observed the occurrence and metastasis of GC.The degree of inflammatory infiltration in pulmonary nodes was observed through hematoxylin-eosin staining.Transwell and wound healing assays were performed for the evaluation of cell invasion and migration ability.Tube formation assay was used for detecting angiogenesis.M2-polarized macrophages were estimated by immunofluorescence and flow cytometry.RESULTS KIF26B was significantly overexpressed in cells and tissues of GC,and the higher expression of KIF26B was related to GC metastasis and prognosis.According to in vivo experiments,KIF26B promoted tumor formation and metastasis of GC.KIF26B expression was positively associated with CAFs’degree of infiltration.Moreover,CAFs could regulate M2-type polarization of macrophages,affecting GC cells’migration,angiogenesis,invasion,and epithelial-mesenchymal transition process.CONCLUSION KIF26B regulated M2 polarization of macrophage through activating CAFs,regulating the occurrence and metastasis of GC.

关键词： metastasis invasion macrophage

Experimental study of face passive failure features of a shallow shield tunnel in coastal backfill sand

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FRONTIERS OF STRUCTURAL AND CIVIL ENGINEERING 2024年第2期18卷 252-271页

作者： Qian, Weifeng huang, ming Wang, Bingnan Xu, Chaoshui Hu, Yanfeng Fuzhou Univ Sch Civil Engn Fuzhou 350108 Peoples R China Univ Adelaide Sch Civil Environm & Min Engn Adelaide 5005 Australia China First Highway Xiamen Engn Co Ltd Xiamen 361000 Peoples R China

Face passive failure can severely damage existing structures and underground utilities during shallow shield tunneling, especially in coastal backfill sand. In this work, a series of laboratory model tests were developed and conducted to investigate such failure, for tunnels located at burial depth ratios for which C/D = 0.5, 0.8, 1, and 1.3. Support pressures, the evolution of failure processes, the failure modes, and the distribution of velocity fields were examined through model tests and numerical analyses. The support pressure in the tests first rose rapidly to the elastic limit and then gradually increased to the maximum value in all cases. The maximum support pressure decreased slightly in cases where C/D = 0.8, 1, and 1.3, but the rebound was insignificant where C/D = 0.5. In addition, the configuration of the failure mode with C/D = 0.5 showed a wedge-shaped arch, which was determined by the outcropping shear failure. The configuration of failure modes was composed of an arch and the inverted trapezoid when C/D = 0.8, 1, and 1.3, in which the mode was divided into lower and upper failure zones.

关键词： tunnel face stability passive failure model test support pressure failure mode STABILITY ANALYSIS CIRCULAR TUNNELS DRIVEN MODEL CENTRIFUGE SEEPAGE SOIL MECHANISMS

Unrecognized congenital heart disease in rural school-age children:getting to the root of the problem

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World Journal of Pediatrics 2022年第5期18卷 305-307页

作者： Qu-ming Zhao Guo-Ying huang Pediatric Heart Center Children's Hospital of Fudan UniversityNational Children's Medical Center399 Wan Yuan RoadShanghai 201102China Shanghai Key Laboratory of Birth Defects ShanghaiChina

Congenital heart disease(CHD)is an important contributor to morbidity and mortality in children,and the global burden of this illness has raised unique challenges to the healthcare system[1,2].China is among the countries with the highest burden of this disorder despite having achieved huge advances in cardiac surgery or interventional therapies in CHD during the past two decades[3].Interestingly and unexpected is the lower prevalence rate of CHD reported in China.The prevalence of CHD at live birth is estimated to be 9.4 per 1000 worldwide[4],However,this figure is much lower in China,with only 4.9 per 1000 live births between 2015 and 2019[5].

关键词： mortality congenital raised

Bioengineered pseudovirus nanoparticles displaying the HA1 antigens of influenza viruses for enhanced immunogenicity

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Nano Research 2022年第5期15卷 4181-4190页

作者： ming Xia Md Rejaul Hoq Pengwei huang Wen Jiang Xi Jiang ming Tan Division of Infectious Diseases Cincinnati Children’s Hospital Medical CenterCincinnatiOH 45229USA Department of Biological Sciences Purdue Cryo-EM FacilityPurdue UniversityIN 47907USA Department of Pediatrics University of Cincinnati College of MedicineCincinnatiOH 45229USA

Even with implementation of current influenza vaccines,influenza still claims up to 500,000 lives worldwide annually,indicating a need for a better vaccine strategy.We have developed a technology to generate unique S_(60)-HA1 pseudovirus nanoparticles(PVNPs)that display the receptor-binding HA1 domains of influenza viruses.Each self-assembled S_(60)-HA1 PVNP consists of a T=1 icosahedral S_(60) nanoparticle that resembles the inner shell of norovirus capsid and 60 surface-displayed HA1 antigens that are excellent vaccine targets.Soluble S_(60)-HA1 PVNPs presenting HA1 antigens of H7N9 influenza virus subtypes have been produced efficiently in large amount.Their three-dimensional(3D)structures have been solved by cryogenic electron microscopy.The PVNP-displayed HA1 antigens react with HA-specific antibody,and retain authentic sialic acid binding specificity and hemagglutinate human erythrocytes.The PVNPs are highly immunogenic,eliciting high titers of HA1-specific antibodies in mice and the mouse sera strongly inhibited hemagglutinations of homologous and heterologous influenza virus HA proteins.Therefore,the S_(60)-HA1 PVNPs may provide useful reagents to study influenza viruses and offer a potential new vaccine tactic to fight the deadly influenza disease.

关键词： S_(60)nanoparticle pseudovirus nanoparticle influenza virus influenza vaccine hemagglutinin norovirus

国医大师黄瑾明基于壮医“谷道浊毒”论治胃肠疾病思路

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时珍国医国药 2023年第9期34卷 2277-2279页

作者：王明杰黄瑾明胡勇覃雁黄贵华广西中医药大学第一附属医院广西南宁530023 广西中医药大学广西南宁530200

“三道两路”是构成壮医学的核心理论,其中谷道是壮医道路理论重要组成部分。壮医学说认为谷道宜畅通不息以运行水谷,使天、地、人三气同步,气血调和,机体健康。“谷道”阻塞不通或调节失度,浊毒沉积胃肠,不仅影响“三气”同步,致脾胃... 详细信息

“三道两路”是构成壮医学的核心理论,其中谷道是壮医道路理论重要组成部分。壮医学说认为谷道宜畅通不息以运行水谷,使天、地、人三气同步,气血调和,机体健康。“谷道”阻塞不通或调节失度,浊毒沉积胃肠,不仅影响“三气”同步,致脾胃气机失衡,发为胃肠疾患,浊毒入血,进一步导致消化系统的危重病症。国医大师黄瑾明从壮医“谷道浊毒”的理论为出发点,临床上运用通腑、祛毒、调气、补虚等壮医内外疗法治疗胃肠病。其治疗思路具体是通胃肠枢机,以调畅整体气机,逐浊毒以推陈致新,补气血复阴阳,以使脾胃气机调和,生机蕴氲,恢复机体的内外平衡和天、地、人三气同步,胃肠道通运畅达,气血生化不绝,从而达到治愈胃肠疾病。

关键词：谷道浊毒胃肠疾病壮医理论临证经验

Assessment of Wet Season Precipitation in the Central United States by the Regional Climate Simulation of the WRFG Member in NARCCAP and Its Relationship with Large-Scale Circulation Biases

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Advances in Atmospheric Sciences 2024年第4期41卷 619-638页

作者： Yating ZHAO ming XUE Jing JIANG Xiao-ming HU Anning huang School of Atmospheric Sciences Nanjing UniversityNanjing 210023China Center for Analysis and Prediction of Storms and School of Meteorology The University of OklahomaNorman 73072U.S.A

Assessment of past-climate simulations of regional climate models(RCMs)is important for understanding the reliability of RCMs when used to project future regional climate.Here,we assess the performance and discuss possible causes of biases in a WRF-based RCM with a grid spacing of 50 km,named WRFG,from the North American Regional Climate Change Assessment Program(NARCCAP)in simulating wet season precipitation over the Central United States for a period when observational data are available.The RCM reproduces key features of the precipitation distribution characteristics during late spring to early summer,although it tends to underestimate the magnitude of precipitation.This dry bias is partially due to the model’s lack of skill in simulating nocturnal precipitation related to the lack of eastward propagating convective systems in the simulation.Inaccuracy in reproducing large-scale circulation and environmental conditions is another contributing factor.The too weak simulated pressure gradient between the Rocky Mountains and the Gulf of Mexico results in weaker southerly winds in between,leading to a reduction of warm moist air transport from the Gulf to the Central Great Plains.The simulated low-level horizontal convergence fields are less favorable for upward motion than in the NARR and hence,for the development of moist convection as well.Therefore,a careful examination of an RCM’s deficiencies and the identification of the source of errors are important when using the RCM to project precipitation changes in future climate scenarios.

关键词： NARCCAP Central United States precipitation low-level jet large-scale environment diurnal variation

Monitoring and identification of spoilage-related microorganisms in braised chicken with modified atmosphere packaging during refrigerated storage

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Food Science and Human Wellness 2023年第1期12卷 28-34页

作者： Yang Lei Yali Zhang Yiqun Cheng Jichao huang ming huang Key Laboratory of Meat Processing and Quality Control Ministry of EducationCollege of Food Science and TechnologyNanjing Agricultural UniversityNanjing 210095China National R&D Center for Poultry Processing Jiangsu Research Center for Livestock and Poultry Products Processing Engineering TechnologyNanjing Huangjiaoshou Food Science and Technology Co.Ltd.Nanjing 211225China College of Environmental Science&Engineering Institute of Functional FoodAnhui Normal UniversityWuhu 241000China College of Engineering Nanjing Agricultural UniversityNanjing 210031China

This study mainly monitored the dominant bacterial populations and identified the spoilage-related microorganisms of braised chicken meat stored under different CO_(2)-modified atmosphere packaging(MAP)during refrigerated storage using a culture-dependent method and 16S rDNA identification.The quality changes and shelf life of the meat were also measured.The growth rate of total viable count(TVC)in braised chicken was slower with an increase of CO_(2) content in MAP,which also occurred in the remaining bacterial species monitored(lactic acid bacteria,Pseudomonas spp.,Brochothrix thermosphacta).The MAP exerted beneficial effects on the quality of braised chicken,as demonstrated by retarding the production of total volatile basic nitrogen(TVB-N)and delaying lipid oxidation(TBARS test).A total of 14 isolates were identified from braised chickens with different packaging at the end of storage,these included P.fragi(6 isolates),P.psychrophila(2 isolates),Enterococcus faecalis(3 isolates),B.thermosphacta(2 isolates),Staphylococcus equorum(1 isolate).

关键词： Braised chicken Modified atmosphere packaging(MAP) 16S rDNA identification Shelf-life