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Aging hallmarks of the primate ovary revealed by spatiotemporal transcriptomics

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Protein & Cell 2024年第5期15卷 364-384页

作者： huifen Lu Ying Jing chen Zhang shuai Ma Weiqi Zhang Daoyuan huang Bin Zhang Yuesheng Zuo Yingying Qin Guang-hui Liu Yang Yu Jing Qu si Wang Advanced Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing 100053China state Key Laboratory of stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijing 100053China state Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of sciences Beijing 100049China Beijing Institute for stem Cell and Regenerative Medicine Beijing 100101China CAs Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China Institute for stem cell and Regeneration CASBeijing 100101China china National Center for Bioinformation Beijing 100101China The Fifth People’s Hospital of Chongqing Chongqing 400062China sino-Danish college University of Chinese Academy of SciencesBeijing 101408China sino-Danish Center for Education and Research Beijing 101408China department of Obstetrics and Gynecology Center for Reproductive MedicinePeking UniversityThird HospitalBeijing 100191China Clinical stem Cell Research Center Peking UniversityThird HospitalBeijing 100191China Center for Reproductive Medicine Cheeloo College of MedicineShandong UniversityJinan 250012China Key Laboratory of Reproductive Endocrinology of Ministry of Education National Research Center for Assisted Reproductive Technology and Reproductive GeneticsShandong Key Laboratory of Reproductive MedicineShandong Provincial Clinical Research Center for Reproductive HealthJinan 250012China Key Laboratory of Organ Regeneration and Reconstruction Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Biomarker Consortium Beijing 100101China

The ovary is indispensable for female reproduction,and its age-dependent functional decline is the primary cause of ***,the molecular basis of ovarian aging in higher vertebrates remains poorly ***,we apply spatiotemporal transcriptomics to benchmark architecture organization as well as cellular and molecular determinants in young primate ovaries and compare these to aged primate *** a global view,somatic cells within the non-follicle region undergo more pronounced transcriptional fluctuation relative to those in the follicle region,likely constituting a hostile microenvironment that facilitates ovarian ***,we uncovered that inflammation,the senescent-associated secretory phenotype,senescence,and fibrosis are the likely primary contributors to ovarian aging(PCOA).Of note,we identified spatial co-localization between a PCOA-featured spot and an unappreciated MT2(Metallothionein 2)highly expressing spot(MT2^(high))characterized by high levels of inflammation,potentially serving as an aging hotspot in the primate ***,with advanced age,a subpopulation of MT2^(high)accumulates,likely disseminating and amplifying the senescent signal *** study establishes the first primate spatiotemporal transcriptomic atlas,advancing our understanding of mechanistic determinants underpinning primate ovarian aging and unraveling potential biomarkers and therapeutic targets for aging and age-associated human ovarian disorders.

关键词： spatial transcriptome primate ovary aging senescence inflammation

Recommendations for the diagnosis and treatment of paroxysmal kinesigemc dyskinesia: an expert consensus in china

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Translational Neurodegeneration 2021年第1期10卷 67-76页

作者： Li Cao Xiaojun huang Ning Wang Zhiying Wu cheng Zhang Weihong Gu shuyan Cong Jianhua Ma Ling Wei Yanchun Deng Qi Fang Qi Niu Jin Wang Zhaoxia Wang You Yin Jinyong Tian shufen Tian Hongyan Bi Hong Jiang Xiaorong Liu Yang Lu Meizhen sun Jianjun Wu Erhe Xu Tao chen Tao chen Xu chen Wei Li shujian Li qinghua Li Xiaonan song Ying Tang Ping Yang Yun Yang Min Zhang Xiong Zhang Yuhu Zhang Ruxu Zhang Yi Ouyang Jintai Yu Quanzhong Hu Qing Ke Yuanrong Yao Zhe Zhao Xiuhe Zhao Guohua Zhao Furu Liang Nan cheng Jianhong Han Rong Peng shengdi chen Beisha Tang department of Neurology Ruijin Hospital Shanghai Jiao Tong University School of MedicineShanghaiChina department of Neurology The Sixth People's Hospital Shanghai Jiaotong UniversityShanghaiChina department of Neurology and Institute of Neurology The First Affiliated HospitalFujian Medical University/Fujian Key Laboratory of Molecular NeurologyFujian Medical UniversityFuzhouChina department of Neurology and Research Center of Neurology The Second Affiliated Hospital/Key Laboratory of Medical Neurobiology of Zhejiang ProvinceZhejiang University School of MedicineHangzhouChina department of Neurology The First Affiliated HospitalSun Yat-sen UniversityGuangdong Provincial Key Laboratory of Diagnosis and Treatment of Major Neurological Diseases/National Key Clinical Department and Key Discipline of NeurologyGuangzhouChina department of Neurology China-Japan Friendship HospitalMovement Disorder and Neurogenetics Research CenterBeijingChina department of Neurology Shengjing Hospital of China Medical UniversityShenyangChina department of Neurology The First Affiliated Hospital of Xinjiang Medical UniversityUrumqiChina department of Neurology The First Affiliated Hospital of Anhui Medical UniversityCollaborative Innovation Center for Neuropsychiatric Disorders and Mental HealthAnhui Medical University/Anhui Province Key Laboratory of Cognition and Neuropsychiatry DisordersHefeiChina department of Neurology Xijing HospitalFourth Military Medical UniversityXi'anChina department of Neurology The First Affiliated Hospital of Soochow UniversitySuzhouChina department of Neurology The First Affiliated Hospital of Nanjing Medical UniversityNanjingChina department of Neurology The First Affiliated Hospital of Guangxi Medical UniversityNanningChina department of Neurology Peking University First HospitalBeijingChina department of Neurology Changzheng Hospitalthe PLA Naval Medical UniversityShanghaiChina department of Neurology and department of Emergency Guizhou Provincial People's Hospital

Paroxysmal dyskinesias are a group of neurological diseases characterized by intermittent episodes of involuntary movements with different *** kinesigenic dyskinesia(PKD)is the most common type of paroxysmal dyskinesia and can be divided into primary and secondary types based on the ***,PKD is characterized by recurrent and transient attacks of involuntary movements precipitated by a sudden voluntary *** major cause of primary PKD is genetic abnormalities,and the inheritance pattern of PKD is mainly autosomal-dominant with incomplete *** proline-rich transmembrane protein 2(PRRT2)was the first identified causative gene of PKD,accounting for the majority of PKD cases *** increasing number of studies has revealed the clinical and genetic characteristics,as well as the underlying mechanisms of *** seeking the views of domestic experts,we propose an expert consensus regarding the diagnosis and treatment of PKD to help establish standardized clinical evaluation and therapies for *** this consensus,we review the clinical manifestations,etiology,clinical diagnostic criteria and therapeutic recommendations for PKD,and results of genetic analyses in PKD patients performed in domestic hospitals.

关键词： Paroxysmal kinesigenic dyskinesia Diagnosis and treatment Expert consensus china

RUNX1-RUNX1T1融合转录本及WT1转录本水平检测的室间比对研究

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中华血液学杂志 2019年第11期40卷 889-894页

作者：秦亚溱朱立文林樉耿素霞刘生伟程辉邬成业肖敏李小青胡瑞萍王丽丽刘海燕马道新关涛叶远馨牛挺岑建农卢利莎孙黎杨同华王云贵李涛王玥李庆华赵晓甦李玲娣陈文敏龙玲玉黄晓军北京大学人民医院北京大学血液病研究所国家血液系统疾病临床医学研究中心100044 北京海思特医学检验实验室有限公司 100176 大连医科大学附属第一医院血液科 116011 广东省人民医院(广东省医学科学院)血液科广州510080 哈尔滨血液病肿瘤研究所 150010 海军军医大学附属长海医院血液内科上海200433 河南省人民医院血液病研究所郑州450003 华中科技大学同济医学院附属同济医院血液科武汉430030 华中科技大学同济医学院附属协和医院干细胞中心武汉430022 吉林大学第一医院血液科长春130021 解放军总医院第一医学中心血液病科北京100853 南通大学附属医院血液科 226001 山东大学齐鲁医院血液科济南250012 山西省肿瘤医院血液科太原030000 四川大学华西医院实验医学科成都610041 四川大学华西医院血液科成都610041 苏州大学附属第一医院江苏省血液研究所国家血液系统疾病临床医学研究中心215006 天津协和华美医学诊断技术有限公司 301617 武汉康圣达医学检验所有限公司 430075 云南省第一人民医院血液科昆明650034 浙江大学医学院附属第一医院血液科杭州310003 郑州大学第一附属医院血液科 450052 中国医科大学附属第一医院沈阳110001 中国医学科学院血液病医院(血液学研究所) 国家血液系统疾病临床医学研究中心天津300020

目的通过室间比对了解国内RUNX1-RUNX1T1融合转录本及WT1转录本检测现状和真实表现。方法北京大学人民医院(简称PKUPH)制备比对样品,即用RUNX1-RUNX1T1(-)患者与RUNX1-RUNX1T1(+)患者新鲜骨髓/外周血有核细胞进行不同比例的稀释,制备出1... 详细信息

目的通过室间比对了解国内RUNX1-RUNX1T1融合转录本及WT1转录本检测现状和真实表现。方法北京大学人民医院(简称PKUPH)制备比对样品,即用RUNX1-RUNX1T1(-)患者与RUNX1-RUNX1T1(+)患者新鲜骨髓/外周血有核细胞进行不同比例的稀释,制备出14种比对样本,每种样本各制备23份平行样本,加入TRIzol均质化后-70℃保存。各家中心采用RT-PCR技术同时检测各样本的RUNX1-RUNX1T1融合转录本及WT1转录本水平,统一以目的基因拷贝数/ABL拷贝数×100%的形式报告结果。通过spearman相关分析计算各家中心与PKUPH检测结果之间的相关系数。结果①RUNX1-RUNX1T1比对:9份为阳性、5份为阴性样本,参与的20家实验室的假阳性率为5%(5/100),假阴性率为0(0/180)。每份阳性样本各家检测值均不相同,9份阳性样本各家报告的结果中位值为0.060%~176.7%,共覆盖3.5个log的范围,各份样本最高与最低报告结果的比值为5.5~12.3(去除1份明显偏离的结果)。85%(17/20)的实验室与PKUPH结果之间的相关系数≥0.98。②WT1比对:14份样本各家报告结果均不相同,中位值为0.16%~67.6%,覆盖2.6个log的范围,各样本检测最高值与最低值的比值为5.3~13.7.62%(13/21)的实验室与PKUPH结果的相关系数≥0.98。③两个转录本每家与PKUPH报告结果的相对关系不一致,2家均低于、7家均高于PKUPH,另11家为1个高于另一个低于PKUPH。结论同一样本各家中心报告的RUNX1-RUNX1T1及WT1转录本水平不同,大多数实验室与PKUPH报告的结果具有很高的一致性,实验室间不同转录本水平的相对关系不一定相同。

关键词：融合蛋白质类,RUNX1-RUNX1T1 WT1 实时聚合酶链反应室间比对

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围产期因素下的多中心儿童脑智发展数据库

中国科学数据:中英文网络版 2023年第3期8卷 114-168页

作者：柯李何清华陈福美苏雪婷徐继红张彩张树东张姝玥安慧萍郭锐岳素萍段雯贾世超杨思佳于延坤赵旸周洋王银山常达陈丽珍范雪如高鹏吕晨钰伍子昀赵云燕全晰赵峰穆艳超闫瑜徐文超刘杰邢丽霞陈晓琴吴翔赵兰锋黄志鹃任颜周郝红艳李慧王婧董青陈利艳黄瑞旺左西年王耘董奇北京师范大学中国基础教育质量监测协同创新中心北京100875 西南大学心理学部重庆400715 解放军疾病预防控制中心军事作业医学防护科北京100071 国家卫生健康委科学技术研究所北京100081 北京师范大学教育学部北京100875 广西师范大学教育学部心理学系桂林541006 安阳市妇幼保健院河南安阳455002 重庆市梁平区人民医院重庆405200 安阳幼儿师范高等专科学校河南安阳456150 北京师范大学认知神经科学与学习国家重点实验室北京100875 北京银河萌芽科技有限公司北京100875 太原理工大学信息与计算机学院太原030002 华南师范大学心理学院广州510631

神经发展性障碍(Neurodevelopmental Disorders,NDDs)是指一系列困扰K12基础教育阶段儿童动作行为发展的精神障碍,其病因及发病机制仍不明确,尚无有效的预测诊疗手段。北京师范大学认知神经科学与学习国家重点实验室依托县/地/区级中心... 详细信息

神经发展性障碍(Neurodevelopmental Disorders,NDDs)是指一系列困扰K12基础教育阶段儿童动作行为发展的精神障碍,其病因及发病机制仍不明确,尚无有效的预测诊疗手段。北京师范大学认知神经科学与学习国家重点实验室依托县/地/区级中心医院采集NDDs高发年龄段儿童脑智发育数据建立的“围产期因素下的多中心儿童脑智发展数据库(Multicenter Database on Perinatal Factors in Child Brain-Mind Development,PeriCBD)”,旨在建立围产期因素下3–10岁儿童脑智发育规律与个体差异相关数据信息平台,为全国脑智发展追踪队列研究、儿童NDDs预测诊疗提供重要基础数据。本文以在河南省安阳市妇幼保健院、重庆市梁平区人民医院已完成的数据采集工作为例,系统介绍PeriCBD组织框架,明确被试行为数据及磁共振成像数据的统一采集、清理、分析流程,数据抽取、转换、加载数据库链接等相关标准化流程,为后续PeriCBD数据采集及数据库使用建立范式。

关键词：围产期神经发展性障碍脑智发展多中心数据库磁共振成像