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In Vitro and In Vivo Evaluation of Antitumor Activity ofLigustrum robustum, A Chinese Herbal Tea

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Chinese Journal of Integrative Medicine 2019年第6期25卷 425-430页

作者： zuo Hao-jiang LIU Shan YAN chun LI Li-man PEI Xiao-fang West China Hospital/West China School of Nursing Sichuan University Chengdu (610041) China Department of Public Health Laboratory Sciences West China School of Public Health Sichuan University Chengdu (610041) China Department of Laboratory Medicine Affiliated Hospital of University of Electr onic Scie nee and Tech no logy of China Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital Chengdu (610072) China The Second Clinical College of Wuhan University Wuhan (430000) China

Objective: To examine the effect of the aqueous extract of Ligustrum robustum on tumor growth in vitro and in vivo and explore the possible molecular mechanisms. Methods: In in vitro study, cell viabilities of human cervical carcinoma cells(HeLa), human breast cancer cells(MCF-7), human prostate cancer cells(PC-3),human hepatoma cells(7721) and human colon carcinoma cells(SW480) were evaluated with cell counting *** L. robustum-treated Hela cells, early or late apoptosis were evaluated by annexin V/PI staining. Mitochondrial membrane potential was measured by staining cells with JC-1. Apoptosis was monitored by nuclear morphology based on chromatin condensation and fragmentation by 4’,6-diamidino-2-phenylinole(DAPI) staining. Caspase-3 and-8 activity levels were measured by a colorimetric assay. In vivo, to evaluate the possible mechanism of L. robustum-mediated antitumor effect, nude mouse xenograft study was also conducted. Results: In in vitro study, L. robustum was found to be toxic to HeLa, MCF-7, PC-3, 7721, SW480, with an half maximal inhibitory concentration value of 2–5 mg/mL(P<0.05). Moreover, externalization of phosphatidylserine, loss of mitochondrial membrane potential, DNA fragmentation and activation of caspase-3 and-8 were detected in L. robustumtreated Hela cells. Using a nude mouse model bearing Hela xenografts, we found that L. robustum reduced tumor volume and tumor weight(P<0.05), but had no effect on body weight and histological damage of important organs. Intraperitoneal injection of L. robustum caused a signi?cant reduction in serum aspartate transaminase and alanine transaminase levels(P<0.05). Furthermore, cleaved caspase-3-positive and terminal nucleotidyl transferase-mediated nick end labeling(TUNEL)-positive cells were observed in L. robustum-treated tumor ***: L. robustum inhibits tumor cell growth both in vitro and in vivo by inducing apoptosis in a caspasedependent way without apparent hepatic toxicity and histol

关键词： Ligustrum robustum antitumor effect Ku-Ding-Cha herbal tea

Genesis of the Binh Do Pb-Zn Deposit in Northern Vietnam:Evidence from H-O-S-Pb Isotope Geochemistry

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Journal of Earth Science 2019年第4期30卷 679-688页

作者： Chaowen huang Huan Li chun-Kit Lai Key Laboratory of Metallogenic Prediction of Nonferrous Metals and Geological Environment Monitoring Ministry of EducationSchool of Geosciences and Info-PhysicsCentral South UniversityChangsha 410083China Faculty of Science Universitiy Brunei Darussalam Gadong BE1410Brunei Darussalam Centre of Excellence in Ore Deposits (CODES) University of TasmaniaHobartTasmania 7001Australia

The Binh Do Pb-Zn deposit in the Phu Luong region(Thai Nguyen Province,northern Vietnam)is located on the southern margin of the South China *** fault-controlled Pb-Zn orebodies are mainly hosted in Upper Paleozoic carbonate *** order to reveal the mineralization type and metallogenesis of this deposit,multi-isotopic(S,Pb,H and O)analyses on typical ore and gangue minerals were *** average ore sulfide δ^34 S∑S value is 4.3‰,suggestive of magmatic *** ore sulfide Pb isotope compositions are homogeneous,with the 206 Pb^204 Pb,207 Pb^204 Pb and 208 Pb^204 Pb values of 18.501 to 18.673,15.707 to 15.798,and 38.911 to 39.428,*** isotope model ages of the ore sulfides(240-220 Ma)are consistent with the timing of regional Triassic S-type granite emplacement(250-220 Ma),suggesting that the metals may have been *** quartz δDV-SMOW(-82.4‰ to-70.5‰)and δ^18 OH2 O(-0.4‰ to +6.4‰)values suggest that the ore-forming fluids were composed of mixed magmatic and meteoric *** with the geological features of the Pb-Zn deposit in the region,we propose that the Pb-Zn deposits belong to magmatic-hydrothermal type,rather than MVT-type as previously *** Triassic granites may have contributed the ore-forming material and heat that drove the hydrothermal *** ore-forming fluids may have migrated into interlayer faults and fractures of the carbonate strata,diluted by subsurface meteoric water and deposited successively the vein-type and stratiform-type Pb-Zn ores.

关键词： Binh Do depposit northernn Vietnam Pbb-Zn belt Triaassic granitoidds isotope geochemistry

A continuing 30-year decline in water quality of Jiaojiang Estuary, China

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Water Science and Engineering 2015年第1期8卷 20-29页

作者： chun-ye Wang Bin Zhou Bei huang College of Marine Science Shanghai Ocean University College of Science Hangzhou Normal University Zhejiang Provincial Zhoushan Ocean Ecological Environment Monitoring Station

A quantitative description of a long-term series of aquatic environmental factors and their spatial distributions was generated using measured data from the Jiaojiang Estuary from 1982 to 2011. The aquatic environmental factors included suspended matter, salinity, and nutrients. Based on these factors, the aquatic ecosystem health in the Jiaojiang Estuary over the last 30 years was analyzed. The results indicated that the suspended matter concentration in the estuary was mainly affected by the amounts of suspended sediment and solid waste, with the value fluctuating over a long period, and the range of high concentration expanded continually; the salinity was mainly affected by precipitation and surface water resources, showing an overall decreasing trend, and the region with low salinity moved seaward and toward the reclamation areas; and the nutritional status, mainly affected by discharge of industrial wastewater and domestic sewage, was satisfactory in the 1980s and 1990s, but the status became severe in recent years. Reclamation had a great influence on these three factors： high reclamation strength led to a significant increase in the suspended matter concentration and a deterioration of the nutritional status, and the reclamation rate was negatively related with the salinity in the estuary. There was a significant positive correlation between the health status of the aquatic ecosystem and salinity, with a correlation coefficient of 0.93. The correlation coefficient between the health status and nutritional status was -0.71, while the correlation between the suspended matter concentration and health status was not as significant as that of the other two factors. The dynamics of the aquatic environment could be divided into four stages： sustainable health from the 1980s to the 1990s, continued deterioration from 2000 to 2003, improvement from 2004 to 2005, and secondary deterioration from 2006 to 2011. The Jiaojiang Estuary is faced with imminent environmental

关键词： Water quality decline Aquatic ecosystem health Aquatic environmental factor Suspended matter concentration Salinity Nutritional status Jiaojiang Estuary

Numerical Simulation on the Resistance Performance of Ice-Going Container Ship Under Brash Ice Conditions

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China Ocean Engineering 2018年第5期32卷 546-556页

作者： GUO chun-yu ZHANG zuo-tian TIAN Tai-ping LI Xia-yan ZHAO Da-gang College of Shipbuilding Engineering Harbin Engineering University SUMEC Marine Co. Ltd.

Ice resistance prediction is a critical issue in the preliminary design of ships navigating brash ice conditions, which is closely related to the safety of a ship to navigate encounter brash ice, and has significant effects on the kinds of propellers and motor power needed. In research on this topic, model tests and full-scale tests on ships have thus far been the primary approaches. In recent years, the application of the finite element method(FEM) has also attracted interest. Some researchers have conducted numerical simulations on ship–ice interactions using the fluid–structure interaction(FSI) method. This study used this method to predict and analyze the resistance of an ice-going ship, and compared the results with those of model ship tests conducted in a towing tank with synthetic ice to discuss the feasibility of the FEM. A numerical simulation and experimental methods were used to predict the brash ice resistance of an ice-going container ship model in a condition with three concentrations of brash ice(60%, 80%, and 90%). A comparison of the results yielded satisfactory agreement between the numerical simulation and the experiments in terms of both observed phenomena and resistance values, indicating that the proposed numerical simulation has significant potential for use in related studies in the future.

关键词： brash ice resistance fluid–structure interaction(FSI) finite element method(FEM) numerical simulation model ship test

Removal of jamming using independent component analysis in non-cooperative passive detection system

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High Technology Letters 2016年第2期22卷 177-182页

作者：应涛 huang Gaoming Shan Hongchang zuo Wei Gao Jun College of Electronic Engineering Naval University of Engineering

Due to electronic jamming transmitted by hostile electromc jamming equtpmcnts tional jamming from other illuminating sources in the complex electromagnetic environment, the per- formance of non-cooperative passive detection systems may degrade it significantly. To solve the problem, a receiving frame with multiple channels for signal preprocessing is designed and a theoret- ical analysis to the received signals in the complex electromagnetic environment is provided. Fur- thermore, a scheme for jamming removal using independent component analysis is proposed. Simula- tion results demonstrate the proposed scheme appears as a very appealing solution for removal of jam- ming and an approximate lOdB signal to distortion ratio over traditional schemes is obtained.

关键词： non-cooperative passive detection system jamming removal independent compo-nent analysis (ICA)

The expression of Lin28B was co-regulated by H3K4me2 and Wnt5a/β-catenin/TCF7L2

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Journal of Integrative Agriculture 2020年第12期19卷 3054-3064页

作者： ZHANG Ya-ni HU Cai WANG Ying-jie zuo Qi-sheng LI Bi-chun Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education Yangzhou UniversityYangzhou 225009P.R.China

Lin28A and Lin28B are homologous RNA-binding proteins that participate in the development of primordial germ cells. The mechanisms underlying expression and regulation of Lin28A have been well documented, but such information for Lin28B is limited. In this study, a fragment of the Lin28B promoter was cloned, the pEGFP-pLin28B vector was constructed. DF-1 chicken fibroblasts were transfected and the expression of green fluorescent protein (GFP) was measured. Furtherly, Lin28B promoter of different lengths fragments was cloned using the chromosome-walking method and the fragments were ligated into the PGL3-Basic vector, and transfected into DF-1 cells. Results of dual-luciferase reporter assay showed that the core of the Lin28B promoter was included in the sequence from –1 431 to –1 034 bp. The binding sites of the transcription factor TCF7L2 was showed within this sequence by bioinformatics analysis. The promoter activity of Lin28B was downregulated (P<0.05) when the TCF7L2 binding site was mutated. Further experiments suggested that Lin28B promoter activity responded to the activation or inhibition of Wnt signaling. Results of chromatin immunoprecipitation and quantitative PCR showed that β-catenin-TCF7L2 may be enriched in the Lin28B promoter core area. In vivo and in vitro activation or inhibition of Wnt signaling significantly up- or down-regulated (P<0.05) Lin28B expression. H3K4me2 enriched in the promoter of Lin28B, which affected the regulation of Wnt signaling to Lin28B. In conclusion, our results showed that H3K4me2 and Wnt5a/β-catenin/TCF7L2 were the positive regulators of Lin28B expression. Findings of this study may lay a theoretical foundation for illuminating the mechanism underlying Lin28B expression.

关键词： primordial germ cells Lin28B promoter H3K4me2 Wnt5a/β-catenin/TCF7L2

Earthquake damage potential and critical scour depth of bridges exposed to flood and seismic hazards under lateral seismic loads

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Earthquake Engineering and Engineering Vibration 2015年第4期14卷 579-594页

作者： Shin-Tai Song chun-Yao Wang Wen-Hsiu huang Department of Civil Engineering Chung-Hsing University Structural Design Department Giant Home Engineering and Consultant

Many bridges located in seismic hazard regions suffer from serious foundation exposure caused by riverbed scour. Loss of surrounding soil significantly reduces the lateral strength of pile foundations. When the scour depth exceeds a critical level, the strength of the foundation is insufficient to withstand the imposed seismic demand, which induces the potential for unacceptable damage to the piles during an earthquake. This paper presents an analytical approach to assess the earthquake damage potential of bridges with foundation exposure and identify the critical scour depth that causes the seismic performance of a bridge to differ from the original design. The approach employs the well-accepted response spectrum analysis method to determine the maximum seismic response of a bridge. The damage potential of a bridge is assessed by comparing the imposed seismic demand with the strengths of the column and the foundation. The versatility of the analytical approach is illustrated with a numerical example and verified by the nonlinear finite element analysis. The analytical approach is also demonstrated to successfully determine the critical scour depth. Results highlight that relatively shallow scour depths can cause foundation damage during an earthquake, even for bridges designed to provide satisfactory seismic performance.

关键词： soil-structure interaction seismic analysis bridge pile foundation scour

A Hybrid Method of Domain Lexicon Construction for Opinion Targets Extraction Using Syntax and Semantics

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Journal of Computer Science & Technology 2016年第3期31卷 595-603页

作者： chun Liao Chong Feng Sen Yang He-Yan huang Department of Computer Science and Technology Beijing Institute of Technology Beijing 100081 China Beijing Engineering Research Center of High Volume Language Information Processing and Cloud Computing Applications Beijing Institute of Technology Beijing 100081 China

Opinion targets extraction of Chinese microblogs plays an important role in opinion mining. There has been a significant progress in this area recently, especially the method based on conditional random field （CRF）. However, this method only takes lexicon-related features into consideration and does not excavate the implied syntactic and semantic knowledge. We propose a novel approach which incorporates domain lexicon with groups of syntactical and semantic features. The approach acquires domain lexicon through a novel way which explores syntactic and semantic information through Part- of-Speech, dependency structure, phrase structure, semantic role and semantic similarity based on word embedding. And then we combine the domain lexicon with opinion targets extracted from CRF with groups of features for opinion targets extraction. Experimental results on COAE2014 dataset show the outperformance of the approach compared with other well-known methods on the task of opinion targets extraction.

关键词： domain lexicon opinion targets extraction syntactic structure semantic role word embedding

Hot Deformation Behaviors in Ti-6Al-4V/(TiB+TiC)Composites

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Acta Metallurgica Sinica(English Letters) 2021年第12期34卷 1747-1757页

作者： Xuejian Lin Hongjun huang Fuyu Dong Yue Zhang Xiaoguang Yuan Bowen Zheng Xiaojiao zuo School of Materials Science and Engineering Shenyang University of TechnologyShenyang 110870China

Thermal compression testing was investigated using the Gleeble 3800 thermal simulator,and thermal deformation behavior of particle-reinforced titanium matrix composites(TMCs)was studied under deformation temperatures of 750-900°C,strain rates of 0.001-1 s^(-1),and experimental deformation of 60%.According to obtained flow stress curves,the hot deformation characteristics were *** on the Arrhenius hyperbolic sinusoidal model,the constitutive equation at high temperature was *** on the theory of dynamic material models,a hot processing map of TMCs at high temperature was established,and the peak region of power dissipation rate and the instability region in the hot processing map were both *** the same time,the corresponding microstructures in the peak power dissipation rate and rheological instability regions were *** results showed that flow stress decreased with increasing deformation temperature and increased with increasing strain *** thermal deformation activation energy of titanium matrix composites was 301.8 kJ/*** Ti-6Al-4V/(TiB+TiC)composites possessed only one instability zone under high-temperature compression at a strain of 0.5,with corresponding temperatures at 750-840°C and strain rates at 0.1-1 s^(-1).The optimal thermal deformation parameters included corresponding temperatures of 830-880°C and strain rates of 0.001-0.05 s^(-1).The microstructures corresponding to optimal hot working parameters in processing maps were more homogeneous than the microstructures in the instability zone,including the distribution uniformity of reinforcement and the degree of dynamic recrystallization,and no instability phenomena including abnormal grain growth,microcracks or intensive fracture of reinforcements were found,indicating that the hot processing map had a positive guiding effect on the option of desirable material thermal-working parameters.

关键词： Ti-6Al-4V/(TiC+TiB)composites Hot deformation Flow stress behavior Processing map