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Mechanism Exploration of Euphorbia fischeriana Steud. for Liver Cancer Based on Aspartic Acid Identification in Metabolomics

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Chinese Journal of Integrative Medicine 2024年第6期30卷 507-514页

作者： ZHAO Jing TIAN Xin-chen ZHANG Jia-qi huang chen SUN Yan QIAO Sen JIANG Shu-long Clinical Medical Laboratory Center Jining First People’s HospitalJiningShandong Province272000China Cheeloo College of Medicine Shandong UniversityJinan250000China

Objective:To investigate the anti-liver cancer effects and aspartic acid(Asp)-related action mechanism of Euphorbia fischeriana Steud.(LD).Methods:The mice model of liver cancer was established by injection of H22 *** 5 days,mice were randomly divided into model group,sorafenib group(20 mg/kg),LD high-dose(LDH,1.36 g/kg) group,LD medium-dose(LDM,0.68 g/kg) group,and LD low-dose(LDL,0.34 g/kg)group,10 mice each *** were intragastrically administered to the mice once daily for 10 days,*** weight,tumor size and tumor weight were *** index was *** changes of liver cancer tissues were evaluated by hematoxylin and eosin staining and TUNEL *** chromatography-mass spectrometer was used to analyze different metabolites between the model and LDH ***:After LD treatment,tumor weight,tumor size and hepatic index were reduced compared with the model *** and karyorrhexis of tumor cells were ***,61 differential metabolites(18 up-regulated,43 down-regulated) were affirmed and 20 pathways of KEGG(P<0.05) were *** addition,Bel-7402,HepG2 and H22 cell viabilities were significantly increased after adding Asp into the *** then,the cell proliferation effect induced by Asp was ameliorated by ***:The anti-liver cancer efficacy of LD extract was validated in H22 mice model,and inhibition of Asp level might be the underlying mechanism.

关键词： liver cancer Euphorbia fischeriana Steud. aspartic acid metabolomics

Complex microstructure fabrication by integrating silicon anisotropic etching and UV-LIGA technology

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High Technology Letters 2007年第4期13卷 369-372页

作者：靖向萌 chen Di huang Chuang chen Xiang Liu Jingquan Zhu Jun National Key Laboratory of Micro/Nano Fabrication Technology. Key Laboratory for Thin Films and Microtechnology of Ministry of Education Research Institute of Micro/Nano Science and Technology Shanghai Jiaotong University Shanghai 200030 P.R. China

A fabrication method which integrates silicon anisotropic etching micromachining with UV-LIGA technology to make complex microstmetures is presented. This proposed combined process enables the fabrication of high-aspect-ratio and three-dimensional （3D） microstructures, which cannot be fabricated by silicon bulk mieromachining or UV-LIGA alone. To demonstrate this combined method, the 100μm thick SU-8 micro gears were fabricated on the silicon convex square structure, which is 100μm × 100μm× 80μm in dimension. In the subsequent micro hot embossing process, a novel type of plastics polyethylene terephtalate glycol （PETG） was tried for use. Through optimizing process parameters, PETG shows the potential of being used as plastic replica in micro-electro-mechanical system （MEMS）. This fabrication technology provides a new option for the increasing need of functionality, quality and economy of MEMS.

关键词： UV-LIGA bulk silicon micro machining 3D microfabrication polyethylene terephtalate glycol (PETG)

Fabrication of Multi-layered Composite Scaffolds by Bi-directional Electrospinning Method

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Journal of Donghua University(English Edition) 2014年第5期31卷 625-629页

作者： WU Tong huang chen chen Jian-feng LIU Wei MO Xiu-mei State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Chemistry Chemical Engineering andBiotechnology Donghua University Shanghai 201620 China College of Textiles Donghua University Shanghai 201620 China College of Materials Science and Engineering Donghua University Shanghai 201620 China

A multi-layered composite scaffolds consisting of poly （ L- ne）（ P （LLA-CL））, collagen （COL） and chitosan （CS） were fabricated by a bi-directional electrospinnlng method. Synthetic P （LLA-CL） was used as the middle layer to enhance the strength, while natural COL/CS blending （9： 1, v/v） was used as the bioactive surfaces （inner and outer layers ） to improve the biocompatibility. Each three transitional layers were set between inner/outer layer and middle layer for delamination resistance. Scanning electron microscopy （SEM） was used to observe the fiber morphology. The Fourier transform infrared attenuated total reflectance spectroscopy （FTIR-ATR） spectra, X- ray diffraction （XRD） and thermogravimetry （TG） tests were used to analyze the physical properties of the scaffolds. The results showed that the modified clectrospinning method bad no negative effect on the components, crystal structure and thermostability of the scaffolds, but could effectively combine the mechanical property of synthetic material and biocompatibility of natural materials. Such method could be applied to the fabrication of composite scaffolds for vascular, skin. and nerve tissue engineering.

关键词： electrospinning poly ( L.lactide-co-ε-caprolactone )( P(LLA-CL) ) collagen chitosan composite nanofibers

Visualization of MF/Diesel RCCI Combustion Process and Soot Emission

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Journal of Thermal Science 2024年第2期33卷 779-792页

作者： huang chen NI Xiao GE Dameng LI Song ZHANG Wanzhi LIU Jinping ZHANG Tingting School of Mechanical and Electronic Engineering Shandong Agricultural UniversityTaian271018China Shandong Provincial Engineering Laboratory of Agricultural Equipment Intelligence Taian271018China Taian Technician Institute Taian271000China Sinotruk Taian Wuyue Special Vehicle Co. Ltd.Taian271000China School of Mechanical Engineering Shaanxi University of TechnologyHanzhong723001China School of Mechanical Engineering Anyang Institute of TechnologyAnyang455000China

Based on the optical engine,the ignition characteristics,combustion process and soot emission characteristics of diesel under different 2-Methylfuran(MF) atmospheres were investigated by high-speed photography and in-cylinder combustion analysis *** results show that at the same main injection timing,the ignition time of reactivity controlled compression ignition(RCCI) combustion mode is earlier than pure diesel combustion,and the ignition point is concentrated near the *** acts as a spark plug to ignite the mixture,but the flame develops slowly in the early stages and the pressure in the cylinder rises *** with pure diesel,RCCI combustion model has smaller peak values of in-cylinder pressure and heat release rate,shorter ignition delay period,earlier combustion phase and shorter combustion *** main spray time at 6℃A BTDC and 12℃A BTDC,with the increase of MF premixing ratio from 0 to 0.75,the peak cylinder pressure decreased by 19.6% and 26% *** addition,with the increase of the MF heat value ratio,the area of KL factor> 1.5 in the combustion chamber decreased and the space integral natural luminescence(SINL) peak value decreased by 48.37%,and the soot formation rate and yield decreased ***,when the MF heat value ratio was too large(75% of the total calorific value),the ignition delay period increased,and misfire occurred at the main injection timing of 0℃A *** RCCI mode of MF/diesel dual fuel has better stability,and better control effect can be obtained at different main inj ection timing.

关键词： 2-methylfuran RCCI visualization two-color method KL factor

Real-time identification of multiple nanoclusters with a protein nanopore in single-cluster level

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Nano Research 2024年第1期17卷 262-269页

作者： Ling Zhang Peilei He huang chen Qingda Liu Libo Li Xun Wang Jinghong Li Department of Chemistry Center for BioAnalytical ChemistryKey Laboratory of Bioorganic Phosphorus Chemistry&Chemical BiologyTsinghua UniversityBeijing 100084China School of Chemistry and Chemical Engineering Guangdong Prov Key Lab Green Chem Prod TechnolSouth China University of TechnologyGuangzhou 510640China New Cornerstone Science Laboratory Shenzhen 518054China Beijing Institute of Life Science and Technology Beijing 102206China Center for BioAnalytical Chemistry Hefei National Laboratory of Physical Science at MicroscaleUniversity of Science and Technology of ChinaHefei 230026China

It is important and challenging to analyze nanocluster structure with atomic ***,α-hemolysin nanopore was used to identify nanoclusters at the single molecule level by providing two-dimensional(2D)dwell time–current blockage spectra and translocation event frequency which sensitively depended on their *** such as Anderson,Keggin,Dawson,and a few lacunary Dawson polyoxometalates with very similar structures,even with only a two-atom difference,could be *** nanopore device could simultaneously measure multiple nanoclusters in a mixture qualitatively and ***,molecular dynamics(MD)simulations provided microscopic understandings of the nanocluster translocation dynamics and yielded 2D dwell time–current blockage spectra in close agreement with *** nanopore platform provides a novel powerful tool for nanocluster characterization.

关键词： nanopore single-molecule nanocluster polyoxometalates real-time detection

A"messenger zone hypothesis"based on the visual three-dimensional spatial distribution of motoneurons innervating deep limb muscles

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Neural Regeneration Research 2024年第7期19卷 1559-1567页

作者： chen huang Shen Wang Jin Deng Xinyi Gu Shuhang Guo Xiaofeng Yin MoE Key Laboratory for Trauma Treatment and Nerve Regeneration Peking UniversityBeijingChina Department of Orthopedics and Trauma Peking University People’s HospitalBeijingChina

Coordinated contraction of skeletal muscles relies on selective connections between the muscles and multiple classes of the spinal motoneuro *** r,current research on the spatial location of the spinal motoneurons innervating differe nt muscles is *** this study,we investigated the spatial distribution and relative position of different motoneurons that control the deep muscles of the mouse hindlimbs,which were innervated by the obturator nerve,femoral nerve,inferior gluteal nerve,deep pe roneal nerve,and tibial *** were visualized by combining a multiplex retrograde tracking technique compatible with three-dimensional imaging of solvent-cleared o rgans(3DISCO)and 3-D imaging technology based on lightsheet fluorescence microscopy(LSFM).Additionally,we propose the hypothesis that"messenger zones"exist as interlaced areas between the motoneuron pools that dominate the synergistic or antagonist muscle *** hypothesize that these interlaced neurons may participate in muscle coordination as messenger *** revealed the precise mutual positional relationships among the many motoneurons that innervate different deep muscles of the *** only do these findings update and supplement our knowledge regarding the overall spatial layout of spinal motoneurons that control mouse limb muscles,but they also provide insights into the mechanisms through which muscle activity is coordinated and the architecture of motor circuits.

关键词： 3-D imaging motoneurons multiple retrograde tracing muscle coordination skeletal muscle spatial distribution optical tissue clearing

Molecular dynamics simulation on DNA translocating through MoS_(2) nanopores with various structures

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Frontiers of Chemical Science and Engineering 2021年第4期15卷 922-934页

作者： Daohui Zhao huang chen Yuqing Wang Bei Li Chongxiong Duan Zhixian Li Libo Li School of Chemistry and Chemical Engineering South China University of TechnologyGuangzhou 510640China School of Chemistry and Chemical Engineering Hubei UniversityWuhan 430062China School of Materials Science and Hydrogen Energy Foshan UniversityFoshan 528231China

The emergence of MoS_(2) nanopores has provided a new avenue for high performance DNA sequencing,which is critical for modem chemical/biological research and ***,molecular dynamics simulations were performed to design a conceptual device to sequence DNA with MoS_(2) nanopores of different structures(e.g.,pore rim contained Mo atoms only,S atoms only,or both Mo and S atoms),where various unfolded single-stranded DNAs(ssDNAs)translocated through the nanopores driven by transmembrane bias;the sequence content was identified by the associating ionic *** ssDNAs adsorbed onto the MoS_(2) surface and translocated through the nanopores by transmembrane electric field in a stepwise manner,where the pause between two permeation events was long enough for the DNA fragments in the nanopore to produce well-defined ionic blockage current to deduce the DNA’s base *** transmembrane bias and DNA-MoS_(2) interaction could regulate the speed of the translocation ***,the structure(atom constitution of the nanopore rim)of the nanopore considerably regulated both the translocate process and the ionic ***,MoS_(2) nanopores could be employed to sequence DNA with the flexibility to regulate the translocation process and ionic current to yield the optimal sequencing performance.

关键词： DNA sequencing MoS_(2) molecular dynamics simulation nanopore ionic current

Ipsilateral and contralateral patent processus vaginalis in pediatric patients with a unilateral nonpalpable testis

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Asian Journal of Andrology 2023年第6期25卷 695-698页

作者： Ming-Ming Yu Hua Xie Yi-chen huang Yi-Qing Lv Fang chen Xiao-Xi Li Department of Urology Shanghai Children’s HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200062China Department of Urology Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai 200233China Shanghai Eastern Urological Reconstruction and Repair Institute Shanghai 200233China

This study aimed to investigate the incidence of patent processus vaginalis(PPV)in pediatric patients with a unilateral nonpalpable testis and explore the associated *** May 2014 to April 2017,152 boys who were diagnosed with a unilateral nonpalpable testis and underwent laparoscopy in Shanghai Children’s Hospital(Shanghai,China)were included in this *** data were collected and reviewed,and the results were analyzed regarding the age at operation,side,development,and position of the nonpalpable *** mean age of the patients was 2.6(standard deviation:2.3)*** testis was absent in 14 cases,nonviable in 81 cases,and viable in 57 *** incidence of PPV was 37.5%(57 of 152)on the ipsilateral side and 16.4%(25 of 152)on the contralateral *** ipsilateral PPV was more prevalent when the nonpalpable testis occurred on the right side(P<0.01).Besides,patients with a viable testis had a greater incidence of ipsilateral PPV than those with a nonviable or absent testis(P<0.01).Moreover,this rate was the highest when the testis was in the abdominal cavity and the lowest when the testis was in the scrotum(both P<0.01).However,the incidence of contralateral PPV was independent of all the *** conclusion,in children with a nonpalpable testis,the incidence of an ipsilateral PPV was significantly related to the side,development,and position of the testis,while it was independent of these factors on the contralateral side.

关键词： children laparoscopy nonpalpable testis patent processus vaginalis

One-step laparoscopic pancreatic necrosectomy verse surgical step-up approach for infected pancreatic necrosis:a case-control study

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World Journal of Emergency Medicine 2022年第4期13卷 274-282页

作者： Sheng-bo Han Ding chen Qing-yong chen Ping Hu Hai Zheng Jin-huang chen Peng Xu Chun-you Wang Gang Zhao Department of Emergency Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Pancreatic Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

BACKGROUND:The surgical step-up approach often requires multiple debridements and might not be suitable for infected pancreatic necrosis(IPN)patients with various abscesses or no safe route for percutaneous catheter drainage(PCD).This case-control study aimed to investigate the safety and effectiveness of one-step laparoscopic pancreatic necrosectomy(LPN)in treating ***:This case-control study included IPN patients undergoing one-step LPN or surgical step-up in our center from January 2015 to December *** short-term and long-term complications after surgery,length of hospital stay,and postoperative ICU stays in both groups were *** and multivariate logistic regression analyses were performed to explore the risk factors of major complications or ***:A total of 53 IPN patients underwent one-step LPN and 37 IPN patients underwent surgical step-up approach in this *** was no significant difference in the incidence of death,major complications,new-onset diabetes,or new-onset pancreatic exocrine insufficiency between the two ***,the length of hospital stay in the one-step LPN group was significantly shorter than that in the surgical step-up *** regression analysis showed that the surgical approach(one-step/step-up)was not the risk factor for major complications or *** logistic regression analysis indicated that computed tomography(CT)severity index,American Society of Anesthesiologists(ASA)class IV,and white blood cell(WBC)were the significant risk factors for major complications or ***:One-step LPN is as safe and effective as the surgical step-up approach for treating IPN patients,and reduces total hospital stay.

关键词： Infected pancreatic necrosis One-step laparoscopic pancreatic necrosectomy Surgical step-up approach