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Global Insight into N-Glycome and N-Glycoproteome of Three Most Abundant Snake Venoms in Asia

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Chemical Research in Chinese Universities 2014年第5期30卷 726-730页

作者： cao Weiqian huang Jiangming cao Jing YANG Pengyuan Institutes of Biomedical Sciences and Department of Chemistry Fudan University Shanghai 200433 P. R. China

Snake venom is a complex cocktail including a variety of biological active proteins and proteinaceous components, which have considerable medical and pharmacological importance. N-Glycosylation is widely impli- cated as a common modification in numerous venom proteins and impacts the in vivo venomic functions. However, systematic survey of N-glycome and N-glycoproteome on snake venoms has not been undertaken. In this study, em- ploying combination of N-glycomics and N-glycoproteomics strategies, we explored the N-glycosylation including both N-glycoproteins and N-glyco-chains in three venoms from Agkistrodon blomhoffii, Naja naja atra Cantor and Vipera russelii siamensis Smith, respectively, which are amongst the most abundant venomous snakes in Asia. As a result, numbers of N-glycoproteins and N-glycans were identified. However, the overlaps of N-glycoproteins and N-glycans of the three venoms were small. Thus, the exploration results of N-glycome and N-glycoproteome indicate that N-glycosylation increases the complexity and variety of the three venoms. Our research provided some new horizons for the comprehensive understanding of venoms variation, which is helpful for the basic venom re- search as well as the management of snake envenomation.

关键词： Glycosylation N-Glycomics N-Glycoproteomics Snake venom

Lentivirus-mediated shRNA interference targeting STAT3 inhibits human pancreatic cancer cell invasion

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World Journal of Gastroenterology 2009年第30期15卷 3757-3766页

作者： Guang Yan Chen huang Jun cao Ke-Jian huang Tao Jiang Zheng-Jun Qiu Department of General Surgery Affiliated First People's Hospital Shanghai Jiao Tong University Shanghai 200080 China

AIM： To investigate RNA interference targeting signal transducer and activator of transcription-3 （STAT3） on invasion of human pancreatic cancer cells.METHODS： We constructed three plasmids of RNA interference targeting the STAT3 gene. After LV （lentivirus）-STAT3siRNA （STAT3 small interfering RNA） the vector was transfected into the human pancreatic cell line, SW1990 and cell proliferation was measured by the MTT assay. Flow cytometry was used to assess cell cycle. Vascular endothelial growth favor （VEGF） and matrix metalloproteinase-2 （MMP-2） mRNA and protein expression were examined by quantitative PCR and western blotting, respectively. The invasion ability of SW1990 cells was determined by cell invasion assay.RESULTS： We successfully constructed the LVSTAT3siRNA lentivirus vector and proved that it can suppress expression of STAT3 gene in SW1990 cells. RNA interference of STAT3 by the LV-STAT3siRNA construct significantly inhibited the growth of SW1990 cells, in addition to significantly decreasing both VEGF and MMP-2 mRNA and protein expression. Moreover, suppression of STAT3 by LV-STAT3siRNA decreased the invasion ability of SW1990 cells.CONCLUSION： The STAT3 signaling pathway may provide a novel therapeutic target for the treatment of pancreatic cancer since it inhibits the invasion ability of pancreatic cancer cells.

关键词： Signal transducer and activator of transcription3 RNA interference Lentivirus vector Pancreatic cancercells Invasion

The Expression and Location of Midkine in Gastric Carcinomas of Chinese Patients

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Cellular & Molecular Immunology 2007年第2期4卷 135-140页

作者： Yaling huang Guochun cao Hui Wang Qingling Wang Yayi Hou Immunology and Reproduction Biology Lab Medical School ＆ State Key Laboratory of Pharmaceutical Biotechnology Nanjing University Nanjing 210093 China

Midkine （MK）, a heparin-binding growth factor, can regulate cell growth, survival and differentiation. MK is expressed at high levels in a variety of human carcinomas. Recently, the urine and serum MK concentration was analyzed in gastric cancer patient. However, the association of the cytokine mRNA expression with the categorical clinicopathological variables of the tumors and the location of its protein expression in the tumor tissues are still elusive. MK mRNA expression from the surgically resected specimens of healthy gastric tissues （9 cases）, gastric cancer tissues and the matched non-cancerons tissues adjacent to the cancer （37 cases） were assessed by reverse transcriptase-polymerase chain reaction （RT-PCR） and real-time PCR. Immunohistochemical analysis was performed to locate MK in gastric cancer. The expression of MK mRNA in gastric cancer was much higher in tumor tissues than that in the non-cancerons tissues and control tissue samples. And its expression was significantly associated with the pTNM stage and distant metastasis, but not with the differentiation grade, tumor size and nodal involvement. MK protein was ubiquitous in the tumor, especially in the adenoid part of tumors. In addition, it was found in the cytoplasm of tumor cells and highly concentrated in nucleus and nucleolns. The expression level and location of MK in gastric tumor tissues of Chinese Patients may be related to the tumor genesis and progression. Further study is necessary on the mechanism of MK in gastric tumorigenesis and tumor growth.

关键词： midkine gastric cancer RT-PCR real-time PCR immunohistochemical analysis

Synthesis of Antiviral Acyclic C-nucleosides Incorporating 4-Thiazolinones Structure

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有机化学 2003年第Z1期23卷 318-319页

作者： huang Yan cao Ling-Hua College of Chemistry and Chemical Engineering Xinjiang University Urumqi 830046 College of Chemistry and Chemical Engineering Xinjiang University Urumqi 830046 State Key Laboratory of Elemento-Organic Chemistry Nankai University Tianjin 300071

　　Carbohydrates are useful molecules to creatures. They take part in life processes in different ways. [ 1] C-nucleo sides are well known nucleoside analogues. A number of nucleoside analogues have been found to sho... 详细信息

关键词： biological activity

Self-doped n-type small molecular electron transport materials for high-performance organic solar cells

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Science China Chemistry 2017年第8期60卷 1136-1144页

作者： jinju liu nannan zheng zhicheng hu zhenfeng wang xiye yang fei huang yong cao Institute of Polymer Optoelectronic Materials and Devices State Key Laboratory of Luminescent Materials and Devices South China University of Technology Guangzhou 510640 China

Two naphthalene diimide （NDI） and perylene diimide （PDI） based n-type water/alcohol soluble small molecules （NFN and PFP） are designed and utilized as electron transport layers （ETLs） for organic solar cells （OSCs）. NFN and PFP are synthesized by using Sonogashira coupling from alkynyl modified fluorene with mono-bromo substituted NDI and PDI. Density functional theory study results of NFN and PFP show that they possess excellent planarity due to the employment of triple bonds as connection units. Moreover, it was shown by electron paramagnetic resonance study that both NFN and PFP possess obvious self-doping behaviors, which may effectively enhance their charge transporting capability as ETLs in OSCs. Power conversion efficiencies of 8.59% and 9.80% can be achieved for OSCs with NFN and PFP as ETLs, respectively. The higher power conversion efficiency （PCE） of PFP based photovoltaic device is originated from the stronger doping property and higher mobility of PFR

关键词： self-doped n-type small molecules organic solar cells electron transport layers

A compilation of known QSOs for the Gaia mission

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Research in Astronomy and Astrophysics 2019年第2期19卷 151-160页

作者： Shi-Long Liao Zhao-Xiang Qi Su-Fen Guo Zi-huang cao Shanghai Astronomical Observatory Chinese Academy of Sciences School of Astronomy and Space Science University of Chinese Academy of Sciences National Astronomical Observatories Chinese Academy of Sciences

Quasars are essential for astrometry in the sense that they are spatially stationary because of their large distance from the Sun. The European Space Agency(ESA) astrometric satellite Gaia is scanning the whole sky with unprecedented accuracy up to the level of a few μas. However, Gaia's two-fields-ofview-observation strategy may introduce a parallax bias in the Gaia catalog. Since it presents no significant parallax, a quasar is the perfect natural object to detect such bias. More importantly, quasars can be used to construct a celestial reference frame in the optical wavelengths for the Gaia mission. In this paper, we compile a list of known quasars that have been published in the literatures. The final compilation(designated as Known Quasars Catalog for Gaia mission, KQCG) contains 1 842 076 objects, among which797 632 objects are found in Gaia DR1 after cross-identifications. Redshift, color information and position uncertainties of the objects are also provided if available. This catalog will be very useful for the Gaia mission.

关键词： quasars:general astrometry parallaxes proper motions reference systems catalogs

Integrated Wireless Communication System Using MANET for Remote Pastoral Areas of Tibet

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China Communications 2016年第4期13卷 49-57页

作者： huang cao ZHOU Qing ZHANG Dongchen School of Information Engineering Xizang Minzu University International school Beijing University of Posts and Telecommunications China Mobile Group Design Institute Co. Ltd.

To reduce the network deployment cost and provide voice, message and low rate data services in remote pastoral areas of Tibet effectively, an integrated wireless communication system utilizing MANET(Mobile Ad hoc Network) is proposed. The sparse mobile devices, assisted with the solar-powered multi-functional standing stations mainly on networking maintenance and routing arrangement, self-organize into a MANET. The topology of the standing stations is designed for networking robust and to simplify the routing method and energy strategy. Then in the OMNe T++(Objective Modular Network Test bed in C++) simulation, the energy consumption is analysis while adjusting routing with the different energy status of the standing stations. The result shows that the standing stations should adjust routing as well as control the mobile devices' activity level according to the energy states of the standing stations and their adjacent mobile devices.

关键词： wireless communication system OMNeT++ MANET Tibet

The role of AGFA high-energy CR in the calibration and quality con-trol of multileaf collimators(MLC)

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Nuclear Science and Techniques 2007年第3期18卷 145-149页

作者： LI Zhaobin XIONG Fei huang Guofeng cao Zheng JIANG Ruiyao FU Shen Department of Radiation Oncology the No.6 Hospital of Shanghai Jiaotong University Shanghai 200233 China

Linear accelerators equipped with multileaf collimators (MLC) are becoming more common and are widely used in the intensity-modulated radiotherapy (IMRT). There is an imperative need to ensure the commissioning specification of the linear accelerators for the sake of quality assurance (QA) and quality control (QC). This paper is aimed to investigate the role of AGFA high-energy CR (Computed Radiography) in calibrating dynamic multileaf col- limators and evaluating the accuracy of the leaf position. The result shows that AGFA high-energy CR can easily and conveniently be used to calibrate MLC and verify its position. Hence, the application of AGFA high-energy CR is proved to be an accurate and time-saving method for routine MLC QC, especially when MLC calibration adjustments are required.

关键词：高能量CR MLC校准质量控制放射疗法

Parasitism-induced effects on host growth and metabolic efficiency in Plutella xylostella larvae parasitized by Cotesia vestalis or Diadegma semiclausum

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Insect Science 2008年第3期15卷 237-243页

作者： Fang huang Ting-Ting cao Min Shi Ya-Feng Chen Xue-xin Chen Institute of Insect Sciences Zhejiang University Hangzhou China

The nutritional physiology of the diamondback moth, Plutella xylostella, larvae was examined after parasitization by the solitary endoparasitoids Cotesia vestalis or Diadegma semiclausum. Examinations were performed in two phases, one was examined at the time point of 24 h post-parasitization, and the other was done at the end of the 4th instar larvae of host. Rates of growth, food consumption, assimilation, excretion, and respiration were calculated as well as approximate digestibility and the rate ratios ECI （percent efficiency of conversion of ingested food to body substance）, and ECD （percent efficiency of conversion of digested food to body substance）. Parasitization by C. vestalis resulted in significant decrease in the rates of growth, feeding, excretion, assimilation, and respiration, but the final dry rate of respiration at the end of last larval stadium was elevated. The ECI and ECD were also reduced as the result of parasitization, but digestibility was increased. All these parameters in the larvae parasitized by D. semiclausum at 24 h post-parasitization were also significantly changed compared to the control; however, these differences were quantitatively, but not qualitatively before pupation, similar to those resulted from parasitization by C. vestalis. In spite of the similarities of the parasitism-induced effects caused by these endoparasitoids, the final metabolic rate, that is, the rate of intake of nutrients required to compensate for metabolism, was much lower in the larvae parasitized by C. vestalis than that of the larvae parasitized by D. semiclausum. All of the results discussed here will contribute toward explaining the different ways these two wasps regulate the parasitoid-host relationship.

关键词： approximate digestibility Cotesia vestalis Diadegma semiclausum foodconsumption Plutella xylostella