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Epiphytic zooplankton community profiles in a typical urban wetland as revealed by DNA metabarcoding

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Journal of Oceanology and Limnology 2024年第5期42卷 1571-1585页

作者： Diwen liang Chunrong huang Senjie LIN Jiahua dong Mingyi liang Hailin LUO State Environmental Protection Key Laboratory of Urban Ecological Simulation and Protection South China Institute of Environmental SciencesMinistry of Ecology and Environment of the People’s Republic of ChinaGuangzhou 510530China Department of Marine Sciences University of ConnecticutGrotonConnecticut 06340USA

Zooplankton,a crucial component of urban wetland,are one of the effective bioindicators for monitoring the feeding stocks of organisms at higher trophic levels and assessing the ecological quality of ***,information about the characteristics of epiphytic zooplankton community structure resulted from traditional methods is limited and hindered by the large amount of detritus and sludge attached to the *** investigated the epiphytic zooplankton communities associated with macrophytes(Vallisneria,Nymphaea,and Thalia dealbata)in a subtropical wetland using as DNA markers of the 18 S rRNA gene and the mitochondrial cytochrome c oxidase subunit I(COI)gene.A total of 241 OTUs of zooplankton were obtained from COI amplicons,including 194 OTUs of Rotifera,22 of Cladocera,and 25 of Copepoda,while only 62 OTUs of zooplankton were obtained from 18 S rDNA amplicons including 34 OTUs of Rotifera and 28 of *** zooplankton communities associated with the three macrophytes were similar,but they differed significantly from those in the open ***,there were no significant temporal differences among the zooplankton *** zooplankton communities were dominated by littoral zooplankton such as Testudinella,Lecane,and ***,especially littoral species,utilize macrophytes as food sources and as refuges against *** further led to an increase inαandβdiversity of zooplankton communities in urban *** result suggests that the joint use of multiple molecular markers could improve the taxonomic resolution and generate a comprehensive biodiversity profile of zooplankton.

关键词： environmental DNA metabarcoding diversity macrophyte cytochrome c oxidase subunit I(COI) 18 S rRNA

甘蔗茎尖组织培养过程酚类物质变化及其与褐变的关系

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南方农业学报 2016年第5期47卷 622-626页

作者：汪淼唐云仙杨柳廖芬黄东亮杨丽涛李杨瑞广西农业科学院甘蔗研究所/中国农业科学院甘蔗研究中心/农业部甘蔗遗传改良生物技术重点实验室/广西甘蔗遗传改良重点实验室南宁530007 广西大学农学院南宁530005 广西农业科学院生物技术研究所南宁530007

【目的】探讨甘蔗茎尖组织培养过程中酚类物质种类及其含量变化与甘蔗茎尖褐变的关系,为甘蔗茎尖离体培养褐变机理研究及解决褐变问题提供理论依据。【方法】利用高效液相色谱对甘蔗茎尖组织培养过程中的酚类物质（绿原酸、没食子酸、... 详细信息

【目的】探讨甘蔗茎尖组织培养过程中酚类物质种类及其含量变化与甘蔗茎尖褐变的关系,为甘蔗茎尖离体培养褐变机理研究及解决褐变问题提供理论依据。【方法】利用高效液相色谱对甘蔗茎尖组织培养过程中的酚类物质（绿原酸、没食子酸、儿茶素、表儿茶素）进行定性和定量分析,研究酚类物质组成和含量在茎尖组织褐变过程中的变化规律及其与褐变的关系。【结果】甘蔗茎尖褐变率增加量随接种时间的延长呈先上升后下降的变化趋势,接种后第3~6 d褐变严重,褐变率增加量在第6 d达峰值（16%）,之后呈下降趋势。接种培养后0~12 d,正常生长和褐变的甘蔗茎尖均检测出没食子酸、绿原酸、儿茶素和表儿茶素4种酚类物质。接种当天4种多酚物质含量均最高;接种后3~6 d,褐变现象严重,褐变率迅速增加,没食子酸、儿茶素和表儿茶素含量显著下降（P〈0.05）。相关性分析结果表明,褐变率增加量与酚类物质含量呈负相关,其中与表儿茶素含量的负相关达极显著水平（P〈0.01）,Pearson相关性系数为0.825。【结论】甘蔗茎尖褐变率增加量与酚类物质含量呈负相关,而表儿茶素为甘蔗茎尖组织培养过程中的主要酚类物质。

关键词：甘蔗茎尖组织培养酚类物质褐变

养殖场牧草周年轮供种植方案初探——以贵州麦坪基地为例

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草业与畜牧 2014年第5期 26-30页

作者：卢敏黄东良李辰琼张锦华贵州省草业研究所贵州贵阳550006 贵州省晴隆县马场乡东良养殖农民专业合作社贵州晴隆561400 贵州省畜牧兽医研究所贵州贵阳550005

针对养殖场全年饲草料的需求状况及现有条件而制定的牧草周年轮供种植方案。该方案从草种选择、各草种及草种组合的种植利用方式和时期、各种草的种植技术要点等方面进行讨论,提出了A、B两个方案,并进行了比较,最终确定B方案以供参考。

关键词：营养特点饲草轮供种植方案

High Fe‑Loading Single‑Atom Catalyst Boosts ROS Production by Density Effect for Efficient Antibacterial Therapy

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Nano-Micro Letters 2025年第2期17卷 187-203页

作者： Si Chen Fang huang Lijie Mao Zhimin Zhang Han Lin Qixin Yan Xiangyu Lu Jianlin Shi Department of Cardiology Shanghai Tenth People’s HospitalSchool of MedicineTongji UniversityShanghai 200092People’s Republic of China Shanghai Institute of Ceramics Chinese Academy of SciencesShanghai 200050People’s Republic of China Shanghai Frontiers Science Center of Nanocatalytic Medicine School of MedicineTongji UniversityShanghai 200092People’s Republic of China

The current single-atom catalysts(SACs)for medicine still suffer from the limited active site ***,we develop a synthetic method capable of increasing both the metal loading and mass-specific activity of SACs by exchanging zinc with *** constructed iron SACs(h^(3)-FNC)with a high metal loading of 6.27 wt%and an optimized adjacent Fe distance of~4 A exhibit excellent oxidase-like catalytic performance without significant activity decay after being stored for six months and promising antibacterial ***,a“density effect”has been found at a high-enough metal doping amount,at which individual active sites become close enough to interact with each other and alter the electronic structure,resulting in significantly boosted intrinsic activity of single-atomic iron sites in h^(3)-FNCs by 2.3 times compared to low-and medium-loading ***,the overall catalytic activity of h^(3)-FNC is highly improved,with mass activity and metal mass-specific activity that are,respectively,66 and 315 times higher than those of commercial Pt/*** addition,h^(3)-FNCs demonstrate efficiently enhanced capability in catalyzing oxygen reduction into superoxide anion(O_(2)·^(−))and glutathione(GSH)*** in vitro and in vivo assays demonstrate the superior antibacterial efficacy of h^(3)-FNCs in promoting wound *** work presents an intriguing activity-enhancement effect in catalysts and exhibits impressive therapeutic efficacy in combating bacterial infections.

关键词： Nanocatalytic medicine Single-atom catalysts Reactive oxygen species(ROS) High metal loading Oxidase catalysis

Longitudinal assessment of peripheral organ metabolism and the gut microbiota in an APP/PS1 transgenic mouse model of Alzheimer’s disease

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Neural Regeneration Research 2025年第10期20卷 2982-2997页

作者： Hongli Li Jianhua huang Di Zhao Lemei Zhu Zheyu Zhang Min Yi Weijun Peng Department of Integrated Traditional Chinese&Western Medicine The Second Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina National Clinical Research Center for Metabolic Diseases ChangshaHunan ProvinceChina Hunan Academy of Chinese Medicine ChangshaHunan ProvinceChina Academician Workstation Changsha Medical UniversityChangshaHunan ProvinceChina

Alzheimer’s disease not only affects the brain,but also induces metabolic dysfunction in peripheral organs and alters the gut *** aim of this study was to investigate systemic changes that occur in Alzheimer’s disease,in particular the association between changes in peripheral organ metabolism,changes in gut microbial composition,and Alzheimer’s disease *** do this,we analyzed peripheral organ metabolism and the gut microbiota in amyloid precursor protein-presenilin 1(APP/PS1)transgenic and control mice at 3,6,9,and 12 months of ***-month-old APP/PS1 mice exhibited cognitive impairment,Alzheimer’s disease-related brain changes,distinctive metabolic disturbances in peripheral organs and fecal samples(as detected by untargeted metabolomics sequencing),and substantial changes in gut microbial composition compared with younger APP/PS1 ***,a strong correlation emerged between the gut microbiota and kidney metabolism in APP/PS1 *** findings suggest that alterations in peripheral organ metabolism and the gut microbiota are closely related to Alzheimer’s disease development,indicating potential new directions for therapeutic strategies.

关键词： Alzheimer’s disease APP/PS1 mice brain-kidney axis gut microbiota heart-brain axis liver-brain axis lung-brain axis microbiota-gut-brain axis peripheral organ metabolism spleen-brain axis

Cholesterol metabolism: physiological versus pathological aspects in intracerebral hemorrhage

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Neural Regeneration Research 2025年第4期20卷 1015-1030页

作者： Ruoyu huang Qiuyu Pang Lexin Zheng Jiaxi Lin Hanxi Li Lingbo Wan Tao Wang Department of Forensic Science School of Basic Medicine and Biological SciencesSuzhou Medicine College of Soochow UniversitySuzhouJiangsu ProvinceChina

Cholesterol is an important component of plasma membranes and participates in many basic life functions,such as the maintenance of cell membrane stability,the synthesis of steroid hormones,and *** plays a key role in the establishment and maintenance of the central nervous *** brain contains 20%of the whole body’s cholesterol,80%of which is located within myelin.A huge number of processes(e.g.,the sterol regulatory element-binding protein pathway and liver X receptor pathway)participate in the regulation of cholesterol metabolism in the brain via mechanisms that include cholesterol biosynthesis,intracellular transport,and *** brain injuries or diseases involving crosstalk among the processes above can affect normal cholesterol metabolism to induce detrimental ***,we hypothesized that cholesterol-related molecules and pathways can serve as therapeutic targets for central nervous system *** hemorrhage is the most severe hemorrhagic stroke subtype,with high mortality and *** cholesterol levels are associated with the risk of intracerebral ***,secondary pathological changes after intracerebral hemorrhage are associated with cholesterol metabolism dysregulation,such as neuroinflammation,demyelination,and multiple types of programmed cell *** cholesterol accumulation in the brain has been found after intracerebral *** this paper,we review normal cholesterol metabolism in the central nervous system,the mechanisms known to participate in the disturbance of cholesterol metabolism after intracerebral hemorrhage,and the links between cholesterol metabolism and cell *** also review several possible and constructive therapeutic targets identified based on cholesterol metabolism to provide cholesterol-based perspectives and a reference for those interested in the treatment of intracerebral hemorrhage.

关键词： cell death cholesterol metabolism intracerebral hemorrhage myelination therapeutic target

Metal-organic frameworks bonded with metal N-heterocyclic carbenes for efficient catalysis

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National Science Review 2022年第6期9卷 64-73页

作者： Chang He Jun liang Yu-huang Zou Jun-dong Yi Yuan-Biao huang Rong Cao State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences University of Chinese Academy of Sciences Science and Technology Innovation Laboratory for Optoelectronic Information of China

Metal N-heterocyclic carbenes(M-NHCs) on the pore walls of a porous metal-organic framework(MOF)can be used as active sites for efficient organic catalysis. Traditional approaches that need strong alkaline reagents or insoluble Ag2O are not, however, suitable for the incorporation of NHCs on the backbones of MOFs because such reagents could destroy their frameworks or result in low reactivity. Accordingly,development of facile strategies toward functional MOFs with covalently bound M-NHCs for catalysis is needed. Herein, we describe the development of a general and facile approach to preparing MOFs with covalently linked active M-NHC(M = Pd, Ir) single-site catalysts by using a soluble Ag salt AgOC(CF3)3as the source and subsequent transmetalation. The well-defined M-NHC-MOF(M = Pd, Ir) catalysts obtained in this way have shown excellent catalytic activity and stability in Suzuki reactions and hydrogen transfer reactions. This provides a general and facile strategy for anchoring functional M-NHC single-site catalysts onto functionalized MOFs for different reactions.

关键词： metal-organic framework N-heterocyclic carbene MIL-101 Suzuki reaction transfer hydrogenation

Sulfolane‑Based Flame‑Retardant Electrolyte for High‑Voltage Sodium‑Ion Batteries

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Nano-Micro Letters 2025年第2期17卷 498-516页

作者： Xuanlong He Jie Peng Qingyun Lin Meng Li Weibin Chen Pei Liu Tao huang Zhencheng huang Yuying Liu Jiaojiao Deng Shenghua Ye Xuming Yang Xiangzhong Ren Xiaoping Ouyang Jianhong Liu Biwei Xiao Jiangtao Hu Qianling Zhang Graphene Composite Research Center College of Chemistry and Environmental EngineeringShenzhen UniversityShenzhen 518060People’s Republic of China Center of Electron Microscopy State Key Laboratory of Silicon and Advanced Semiconductor MaterialsSchool of Materials Science and EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China GRINM(Guangdong)Research Institute for Advanced Materials and Technology FoshanGuangdong 528051People’s Republic of China College of Energy Engineering Zhejiang UniversityHangzhouZhejiang 310027People’s Republic of China School of Materials Science and Engineering Xiangtan UniversityXiangtan 411105People’s Republic of China Shenzhen Eigen-Equation Graphene Technology Co.Ltd Shenzhen 518000People’s Republic of China

Sodium-ion batteries hold great promise as next-generation energy storage ***,the high instability of the electrode/electrolyte interphase during cycling has seriously hindered the development of *** particular,an unstable cathode–electrolyte interphase(CEI)leads to successive electrolyte side reactions,transition metal leaching and rapid capacity decay,which tends to be exacerbated under high-voltage ***,constructing dense and stable CEIs are crucial for high-performance *** work reports localized high-concentration electrolyte by incorporating a highly oxidation-resistant sulfolane solvent with non-solvent diluent 1H,1H,5H-octafluoropentyl-1,1,2,2-tetrafluoroethyl ether,which exhibited excellent oxidative stability and was able to form thin,dense and homogeneous *** excellent CEI enabled the O3-type layered oxide cathode NaNi_(1/3)Mn_(1/3)Fe_(1/3)O_(2)(NaNMF)to achieve stable cycling,with a capacity retention of 79.48%after 300 cycles at 1 C and 81.15%after 400 cycles at 2 C with a high charging voltage of 4.2 *** addition,its nonflammable nature enhances the safety of *** work provides a viable pathway for the application of sulfolane-based electrolytes on SIBs and the design of next-generation high-voltage electrolytes.

关键词： Sodium-ion batteries Sulfolane-based electrolyte High voltage Layered oxide cathode Flame retardant

Relative difficulty distillation for semantic segmentation

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Science China(Information Sciences) 2024年第9期67卷 126-145页

作者： dong liang Yue SUN Yun DU Songcan CHEN Sheng-Jun huang MIIT Key Laboratory of Pattern Analysis and Machine Intelligence College of Computer Science and Technology Nanjing University of Aeronautics and Astronautics Shenzhen Research Institute Nanjing University of Aeronautics and Astronautics

Current knowledge distillation(KD) methods primarily focus on transferring various structured knowledge and designing corresponding optimization goals to encourage the student network to imitate the output of the teacher network. However, introducing too many additional optimization objectives may lead to unstable training, such as gradient conflicts. Moreover, these methods ignored the guidelines of relative learning difficulty between the teacher and student networks. Inspired by human cognitive science, in this paper, we redefine knowledge from a new perspective — the student and teacher networks' relative difficulty of samples, and propose a pixel-level KD paradigm for semantic segmentation named relative difficulty distillation(RDD). We propose a two-stage RDD framework: teacher-full evaluated RDD(TFE-RDD) and teacher-student evaluated RDD(TSE-RDD). RDD allows the teacher network to provide effective guidance on learning focus without additional optimization goals, thus avoiding adjusting learning weights for multiple losses. Extensive experimental evaluations using a general distillation loss function on popular datasets such as Cityscapes, Cam Vid, Pascal VOC, and ADE20k demonstrate the effectiveness of RDD against state-ofthe-art KD methods. Additionally, our research showcases that RDD can integrate with existing KD methods to improve their upper performance bound. Codes are available at https://***/sunyueue/***.

关键词： knowledge distillation semantic segmentation relative difficulty sample weighting prediction discrepancy