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Establishment of a Multiplex Detection Method for Common Bacteria in Blood Based on Human Mannan-Binding Lectin Protein-Conjugated Magnetic Bead Enrichment Combined with Recombinase-Aided PCR Technology

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Biomedical and Environmental Sciences 2024年第4期37卷 387-398页

作者： ZHAO Zi Jin CHEN Xiao Ping hua shao wei LI Feng Yu ZHAO Meng XING Chen Hao WANG Jie TIAN Feng Yu ZHANG Rui Qing LYU Xiao Na HAN Zhi Qiang WANG Yu Xin LI Hong Yi SHEN Xin Xin MA Xue Jun TIE Yan Qing Graduate School Hebei North UniversityZhangjiakou 075000HebeiChina Department of Clinical Laboratory Hebei General HospitalShijiazhuang 050051HebeiChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases NHC Key Laboratory of Medical Virology and Viral DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China Department of Clinical Laboratory Pathology Diagnostic Center Shiyan General HospitalShiyan 442000HubeiChina Graduate School Hebei Medical University Shijiazhuang 050031HebeiChina

Objective Recombinase-aided polymerase chain reaction(RAP)is a sensitive,single-tube,two-stage nucleic acid amplification *** study aimed to develop an assay that can be used for the early diagnosis of three types of bacteremia caused by Staphylococcus aureus(SA),Pseudomonas aeruginosa(PA),and Acinetobacter baumannii(AB)in the bloodstream based on recombinant human mannanbinding lectin protein(M1 protein)-conjugated magnetic bead(M1 bead)enrichment of pathogens combined with *** Recombinant plasmids were used to evaluate the assay *** blood influenza bacteria were used for the specific *** and clinical plasma samples were enriched with M1 beads and then subjected to multiple recombinase-aided PCR(M-RAP)and quantitative PCR(qPCR)*** analysis was used to evaluate the consistency between the two *** The M-RAP method had sensitivity rates of 1,10,and 1 copies/μL for the detection of SA,PA,and AB plasmids,respectively,without cross-reaction to other bacterial *** M-RAP assay obtained results for<10 CFU/mL pathogens in the blood within 4 h,with higher sensitivity than qPCR.M-RAP and qPCR for SA,PA,and AB yielded Kappa values of 0.839,0.815,and 0.856,respectively(P<0.05).Conclusion An M-RAP assay for SA,PA,and AB in blood samples utilizing M1 bead enrichment has been developed and can be potentially used for the early detection of bacteremia.

关键词： Staphylococcus aureus Pseudomonas aeruginosa Acinetobacter baumannii Human Mannan-binding lectin protein Bloodstream infection Recombinase-aided PCR assay Multiple detection

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An analytical approach to modeling stress-strain relationship of particle-reinforced metal matrix composites

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Rare Metals 2022年第11期41卷 3903-3910页

作者： Zhao-Bing Xiang Jun-Hui Nie shao-hua wei Tao Zuo Jian-Zhong Fan National Engineering Technology Research Center for Nonferrous Metals Composites General Research Institute for Nonferrous MetalsBeijing100088China

An analytical model was established to simulate the stress-strain curves of particle-reinforced metal matrix composites by the classical stress equilibrium equation and secant modulus method,where particle cracking and matrix tearing were taken into *** stress-strain curves of the composites predicted by the model fit the experimental curves very *** stressstrain relationship of the matrix affected by the particles is predicted exactly,and it is found that the yield stress of the matrix is influenced obviously by the particle *** yield strength of the composites is also predicted by the model,and the results fit the experimental data very well.

关键词： Strengthening mechanism Yield strength Particle fracture Matrix stress-strain curve

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Adenomyoma of the distal common bile duct demonstrated by endoscopic ultrasound: A case report and review of the literature

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World Journal of Clinical Cases 2019年第21期7卷 3615-3621页

作者： Li-Ming Xu Duan-Min Hu Wen Tang shao-hua wei wei Chen Guang-Qiang Chen Department of Gastroenterology The Second Affiliated Hospital of Soochow UniversitySuzhou 215004Jiangsu ProvinceChina Endoscopy Center The Second Affiliated Hospital of Soochow UniversitySuzhou 215000Jiangsu ProvinceChina Department of Hepatological Surgery The Second Affiliated Hospital of Soochow UniversitySuzhou 215000Jiangsu ProvinceChina Imaging Department The Second Affiliated Hospital of Soochow UniversitySuzhou 215000Jiangsu ProvinceChina

BACKGROUND Adenomyomatous hyperplasia of the distal common bile duct(CBD)is very rare,with only scarce case reports in the *** is usually based on imaging findings,and endoscopic biopsy is very difficult before *** is believed that adenomyomatous hyperplasia has little or no risk of malignant *** SUMMARY A 68-year-old woman with abdominal pain in the right upper quadrant was referred to our *** ultrasonography in the emergency ward revealed acute cholecystitis and dilated *** findings showed elevated levels of transaminases,phosphatase,andγ-*** treatment for 3 d did not relieve the *** resonance cholangiopancreatography(MRCP)and computed tomography(CT)showed proximal bile duct dilatation but could not identify the *** ultrasonography(EUS)demonstrated a mixed echogenic mass in the distal *** surgery,a firm mass was found in the distal CBD and the Whipple procedure was performed with the initial concern of *** showed diffuse adenomyomatous *** EUS may be a useful choice to diagnose adenomyoma of the distal CBD before operation,especially in patients with ambiguous MRCP/CT findings.

关键词： Adenomyoma Common bile duct Endoscopic ultrasound Diagnosis Casereport

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Autofocus by Lissajous scanning in time reversal optical scanning holography

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CHINESE OPTICS LETTERS 2024年第8期22卷 080501-080501页

作者： Liu, Jie Ou, Haiyan Wang, hua Peng, Lin shao, wei Univ Elect Sci & Technol China Shenzhen Inst Adv Study Shenzhen 518000 Peoples R China Univ Elect Sci & Technol China Sch Phys Chengdu 611731 Peoples R China Guilin Univ Elect Technol Key Lab Cognit Radio & Informat Proc Guilin 541004 Peoples R China

In this Letter, an autofocusing method in optical scanning holography (OSH) system is proposed. By introducing Lissajous scanning into multiple signal classification (MUSIC) method in time-reversal (TR) OSH, the axial locations of the targets can be retrieved with better resolution and the peak prominence increases from 0.21 to 0.34. The feasibility of this method is confirmed by simulation as well as experiment.

关键词： digital holography Lissajous scanning image reconstruction axial localization SECTIONAL IMAGE-RECONSTRUCTION SCATTERING MICROSCOPY TARGETS

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Advanced hermetic electronic packaging based on lightweight silicon/aluminum composite produced by powder metallurgy technique

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Rare Metals 2020年第11期39卷 1307-1313页

作者： Yan-Qiang Liu Jian-Zhong Fan Xin-Xiang Hao shao-hua wei Jun-Hui Nie Zi-Li Ma Ming-Kun Liu Ya-Bao Wang National Engineering and Technology Research Center for Nonferrous Metals Composites General Research Institute for Nonferrous MetalsBeijing100088China

Silicon/aluminum(Si/Al)composite is a kind of lightweight electronic packaging material that received a lot of attention in the past 20 *** this paper,a series of Si/Al composites with lowered coefficient of thermal expansion(CTE)and high thermal conductivity were produced by powder metallurgy(PM).The Si/Al composites are fully dense and have fine Si particles uniquely distributed within pure Al *** 50%Si/Al composites were designed to have strength in the range of 185-290 MPa to meet different demands,while the other properties keep *** toughness of the composites is measured to be 9-10 MPa·m^(1/2).The composites were machined to 50%Si/Al housings and 27%Si/Al *** the hermeticities of housings before and after laser-beam-welding sealing are *** measured leak rate of composites and sealed housings is in magnitude order of 1×10^(-10)and 1×10^(-9)Pa·m^(3)·s^(-1),respectively,suggesting high *** good hermeticity is attributed to the full dense materials,good weldability,and extremely low weld *** present Si/Al composites are expected to be extensively used in highly hermetic electronic packages.

关键词： Si/Al composite Electronic packaging Strength Hermetic Powder metallurgy

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Atroposelective Synthesis of 2-Arylindoles via Chiral Phosphoric Acid-Catalyzed Direct Amination of Indoles

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Chinese Journal of Chemistry 2024年第7期42卷 731-735页

作者： Wen Bao Ye-Hui Chen Yu-wei Liu shao-hua Xiang Bin Tan School of Pharmacy&StateKey Laboratory of Applied Organic Chemistry Lanzhou UniversityLanzhouGansu 730000China Shenzhen Grubbs Institute Department of ChemistrySouthern University of Science and TechnologyShenzhenGuangdong 518055China

Indole-based atropisomers are a very important class of axially chiral ***,the atroposelective synthesis of axially chiral 2-arylindole remains largely *** this study,we report the successful synthesis of atropisomeric 2-arylindoles using direct amination of indoles with p-quinonediimines in the presence of chiral phosphoric acid as a *** acts as an aminating reagent through formal polarity inversion of *** malonate group on the 2-aryl of 2-indoles was found to be essen-tial for high enantioselectivity of the *** could be due to the additional interaction between the ester group and the cata-lyst,as well as the intramolecular hydrogen *** findings provide a new strategy for the asymmetric construction of 2-arylindole atropisomers.

关键词： Chiral phosphoric acid Quinonediimines Direct amination Nucleophilic addition Asymmetric catalysis Enantioselectivity Axially chiral 2-arylindole Atroposelective synthesis

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Aerial target threat assessment based on gated recurrent unit and self-attention mechanism

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Journal of Systems Engineering and Electronics 2024年第2期35卷 361-373页

作者： CHEN Chen QUAN wei shao Zhuang School of Automation Beijing Institute of TechnologyBeijing 100081China State Key Laboratory of Intelligent Control and Decision of Complex Systems Beijing 100081China

Aerial threat assessment is a crucial link in modern air combat, whose result counts a great deal for commanders to make decisions. With the consideration that the existing threat assessment methods have difficulties in dealing with high dimensional time series target data, a threat assessment method based on self-attention mechanism and gated recurrent unit(SAGRU) is proposed. Firstly, a threat feature system including air combat situations and capability features is established. Moreover, a data augmentation process based on fractional Fourier transform(FRFT) is applied to extract more valuable information from time series situation features. Furthermore, aiming to capture key characteristics of battlefield evolution, a bidirectional GRU and SA mechanisms are designed for enhanced ***, after the concatenation of the processed air combat situation and capability features, the target threat level will be predicted by fully connected neural layers and the softmax classifier. Finally, in order to validate this model, an air combat dataset generated by a combat simulation system is introduced for model training and testing. The comparison experiments show the proposed model has structural rationality and can perform threat assessment faster and more accurately than the other existing models based on deep learning.

关键词： target threat assessment gated recurrent unit(GRU) self-attention(SA) fractional Fourier transform(FRFT)

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Intelligent diagnosis of retinal vein occlusion based on color fundus photographs

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International Journal of Ophthalmology(English edition) 2024年第1期17卷 1-6页

作者： Yu-Ke Ji Rong-Rong hua Sha Liu Cui-Juan Xie shao-Chong Zhang wei-hua Yang Eye Hospital Nanjing Medical UniversityNanjing 210000Jiangsu ProvinceChina College of Electronic Information Engineering Nanjing University of Aeronautics and AstronauticsNanjing 210000Jiangsu ProvinceChina Shenzhen Eye Institute Shenzhen Eye HospitalJinan UniversityShenzhen 518000Guangdong ProvinceChina

AIM:To develop an artificial intelligence(AI)diagnosis model based on deep learning(DL)algorithm to diagnose different types of retinal vein occlusion(RVO)by recognizing color fundus photographs(CFPs).METHODS:Totally 914 CFPs of healthy people and patients with RVO were collected as experimental data sets,and used to train,verify and test the diagnostic model of *** the images were divided into four categories[normal,central retinal vein occlusion(CRVO),branch retinal vein occlusion(BRVO),and macular retinal vein occlusion(MRVO)]by three fundus disease *** Transformer was used to build the RVO diagnosis model,and different types of RVO diagnosis experiments were *** model’s performance was compared to that of the ***:The accuracy of the model in the diagnosis of normal,CRVO,BRVO,and MRVO reached 1.000,0.978,0.957,and 0.978;the specificity reached 1.000,0.986,0.982,and 0.976;the sensitivity reached 1.000,0.955,0.917,and 1.000;the F1-Sore reached 1.000,0.9550.943,and 0.887 *** addition,the area under curve of normal,CRVO,BRVO,and MRVO diagnosed by the diagnostic model were 1.000,0.900,0.959 and 0.970,*** diagnostic results were highly consistent with those of fundus disease experts,and the diagnostic performance was ***:The diagnostic model developed in this study can well diagnose different types of RVO,effectively relieve the work pressure of clinicians,and provide help for the follow-up clinical diagnosis and treatment of RVO patients.

关键词： deep learning artificial intelligence Swin Transformer diagnostic model retinal vein occlusion color fundus photographs

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Diabetic retinopathy identification based on multi-sourcefree domain adaptation

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International Journal of Ophthalmology(English edition) 2024年第7期17卷 1193-1204页

作者： Guang-hua Zhang Guang-Ping Zhuo Zhao-Xia Zhang Bin Sun wei-hua Yang shao-Chong Zhang School of Big Data Intelligent Diagnosis&Treatment Industry Taiyuan UniversityTaiyuan 030032Shanxi ProvinceChina Shanxi Key Laboratory of Intelligent Optimization Computing and Blockchain Technology Taiyuan Normal UniversityTaiyuan 030619Shanxi ProvinceChina Shanxi Eye Hospital Taiyuan 030002Shanxi ProvinceChina College of Computer Science and Technology Taiyuan Normal UniversityTaiyuan 030619Shanxi ProvinceChina Shenzhen Eye Institute Shenzhen Eye HospitalJinan UniversityShenzhen 518040Guangdong ProvinceChina

AIM:To address the challenges of data labeling difficulties,data privacy,and necessary large amount of labeled data for deep learning methods in diabetic retinopathy(DR)identification,the aim of this study is to develop a source-free domain adaptation(SFDA)method for efficient and effective DR identification from unlabeled ***:A multi-SFDA method was proposed for DR *** method integrates multiple source models,which are trained from the same source domain,to generate synthetic pseudo labels for the unlabeled target ***,a softmax-consistence minimization term is utilized to minimize the intra-class distances between the source and target domains and maximize the inter-class *** is performed using three color fundus photograph datasets(APTOS2019,DDR,and EyePACS).RESULTS:The proposed model was evaluated and provided promising results with respectively 0.8917 and 0.9795 F1-scores on referable and normal/abnormal DR identification *** demonstrated effective DR identification through minimizing intra-class distances and maximizing inter-class distances between source and target ***:The multi-SFDA method provides an effective approach to overcome the challenges in DR *** method not only addresses difficulties in data labeling and privacy issues,but also reduces the need for large amounts of labeled data required by deep learning methods,making it a practical tool for early detection and preservation of vision in diabetic patients.

关键词： diabetic retinopathy multisource-free domain adaptation pseudo-label generation softmaxconsistence minimization

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Thermodynamic nature of black holes in coexistence region

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Science China(Physics,Mechanics & Astronomy) 2024年第5期67卷 27-32页

作者： shao-Wen wei Yu-Xiao Liu Lanzhou Center for Theoretical Physics Key Laboratory of Theoretical Physics of Gansu Provinceand Key Laboratory of Quantum Theory and Applications of Mo ELanzhou UniversityLanzhou 730000China Institute of Theoretical Physics&Research Center of Gravitation Lanzhou UniversityLanzhou 730000China

Studying the system state of coexistence regions will peek into to reveal microscopic interactions between different phases of a thermodynamic ***,there is no effective method to study thermodynamic nature of the coexistence black hole regions for the failure of the equation of *** at these coexistence states,in this work,we develop a general approach by introducing two new ratio *** first one is the ratio of the horizon radii of the saturated coexistence small and large black holes,and the second one measures that of the small black hole molecule number to the total molecule *** demonstrate that the first parameter can serve as an order parameter to characterize the first-order phase *** study also shows that the black hole state in the coexistence region is uniquely determined by these two introduced parameters bounded between 0 and *** results are quite significant in the analytical study of phase transition and the microscopic nature of black hole in the coexistence regions.

关键词： transition. state. thermodynamic

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