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Evaluating the Mechanism of Tropical Expansion Using Idealized Numerical Experiments

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Ocean-Land-Atmosphere Research 2023年第1期2卷 44-56页

作者： hu Yang Gerrit Lohmann xiaoxu Shi Juliane Müller Alfred Wegener Institute Helmholtz Centre for Polar and Marine ResearchBremerhavenGermany University of Bremen Center for Marine Environmental SciencesMARUMBremenGermany

A wide range of evidence reveals that the tropical belt is *** mechanisms have been proposed to contribute to this expansion,some of which even contradict each *** study of Yang et *** that the poleward advancing mid-latitude meridional temperature gradient(MTG),originating from enhanced subtropical ocean warming,plays a leading role in driving tropical ***,the abrupt4xCO_(2) experiment indicates that tropical expansion occurs at a faster rate than is indicated by changes related to ocean temperature *** idealized amip4K experiment illustrates that without introducing any ocean warming pattern,uniform ocean surface warming also drives tropical *** results based on these idealized experiments seem to contradict the hypothesis proposed by Yang et *** this study,we revisit these 2 experiments and show that both experiments actually support the hypothesis that MTG migration is driving tropical *** specifically,in the abrupt4xCO_(2) experiment,although the rate of ocean warming is relatively slow,the poleward shift of the MTG is as rapid as tropical *** the amip4K experiment,although ocean surface warming is uniform,the heating effect of the ocean on the atmosphere is nonuniform because of the nonlinear relationship between temperature,evaporation,and thermal *** nonuniform oceanic heating to the atmosphere introduces a poleward shift of the MTG within the upper troposphere and drives a shift in the jet *** conducting an additional idealized experiment in which tropical expansion occurs under both a migrating MTG and a cooling climate,we argue that the migration of the MTG,rather than global warming,is the key mechanism in driving tropical expansion.

关键词： warming ocean tropical

乙型脑炎病毒的实时荧光定量逆转录PCR检测方法的建立

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分子诊断与治疗杂志 2017年第5期9卷 341-346页

作者：孙莉陈路王华贵赵相胜胡小许迟洪霞祁荣吴伟立华北石油管理局总医院检验科河北任丘062552 北京宏微特斯生物科技有限公司北京101300 中国科学院北京基因组研究所北京100101 华北石油管理局总医院消化科河北任丘062552

目的建立一种可以准确、快速、特异检测乙型脑炎病毒(Japanese encephalitis virus,JEV)的一步法实时荧光定量逆转录PCR(reverse transcription-quantitative real time PCR,RT-q PCR)体系。方法参照NCBI数据库的乙脑病毒全基因组序列,经过一致性比对分析,在乙脑病毒保守的3′非翻译区(3′untranslated region,3′UTR)区段,设计相应的特异性引物以及Taqman-MGB探针,优化检测体系及反应条件,建立一步法RT-q PCR方法。利用登革热、森林脑炎病毒、肠道病毒71型(EV71)、柯萨奇病毒A16型(Cox A16)、狂犬病毒培养物和临床诊断为登革热及手足口病人的血清样本检测其交叉反应。选择15例疑似脑炎症状的病人血清样本,以国家食品药品监督管理总局(CFDA)批准的乙脑病毒检测试剂盒作对照试剂盒,检测其临床适用性。结果所建立的乙脑病毒一步法RT-q PCR检测方法重复性试验Ct值的变异系数CV在0.46%~0.53%之间,最低可检出5 copies/反应样本。检测体系与登革热、森林脑炎病毒等黄病毒及EV71、Cox A16、狂犬病毒培养物等未发现交叉反应;64份临床诊断为登革热和手足口病人的血清样本检测也均为阴性。15份疑似乙脑感染的病人血清样本的检测结果与国家食品药品监督管理总局批准的乙型脑炎病毒检测试剂盒检测结果一致,但本方法操作步骤更少,检测时间更短。结论本实验建立的乙型脑炎病毒一步法实时荧光RT-q PCR检测方法具有较高灵敏度、特异性,可用于乙型脑炎病毒的检测。

关键词：乙型脑炎病毒 Taqman-MGB 流行性乙型脑炎 RT-qPCR

754例Ⅰ期-Ⅲa期可手术切除非小细胞肺癌患者EGFR和KRAS基因突变状态及其临床意义

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中国肺癌杂志 2017年第9期20卷 617-622页

作者：赵静高洁郭李平胡晓许刘琦赵金银刘利成姜君王孟昭梁智勇徐燕陈闽江张力李龙芸钟巍中国医学科学院北京协和医学院北京协和医院呼吸科北京100730 中国医学科学院北京协和医学院北京协和医院病理科北京100730 大连晶泰医学检验所大连116635 北京宏微特斯生物科技有限公司北京101300

背景与目的表皮生长因子受体(epidermal growth factor receptor,EGFR)和KRAS基因是非小细胞肺癌(non-small cell lung cancer,NSCLC)重要的分子靶点,但目前研究主要集中在晚期NSCLC组织和血浆标本的EGFR检测,早期NSCLC组织样本中EGFR和KRAS突变特征尚不清楚。本研究将探讨I期-IIIa期NSCLC EGFR和KRAS基因突变与相关临床病理特征的关系。方法采用突变扩增系统(amplification refractory mutation system,ARMS)PCR方法检测北京协和医院病理科提供的754例I期-IIIa期NSCLC组织样本的EGFR和KRAS基因突变状况,分析基因突变率及其与临床病理特征的关系。结果 EGFR和KRAS基因热点突变的突变率分别为34.5%和13.1%,其中有3例样本具有EGFR和KRAS基因的双突变。EGFR基因在女性中的突变率高于男性(39.5%vs 29.4%,P=0.076),在腺癌中的突变率(38.7%)高于鳞癌、腺鳞癌、大细胞癌(P<0.01),但仍明显低于其他研究报道的亚裔晚期腺癌突变率(-50%)。KRAS基因突变在男性中的突变率高于女性(16.6%vs 9%,P=0.048),且在腺癌中的突变率也高于其他类型,但差异不显著(P=0.268)。与KRAS基因突变阳性组相比,EGFR基因突变阳性组在年龄分布上有年轻化的趋势(P=0.031,5),在性别分布上有显著性差异(P<0.01)。结论 I期-IIIa期NSCLC EGFR基因突变率较晚期患者低,且EGFR和KRAS基因双突变的发生率为0.9%。

关键词：肺肿瘤 EGFR基因 KRAS基因可手术切除标本

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一起35kV开关柜电缆终端故障案例分析

黑龙江电力 2022年第5期44卷 441-444页

作者：张德文张朋胡晓旭魏新宇徐嘉明张光中国网黑龙江省电力有限公司电力科学研究院哈尔滨150030 国网黑龙江省电力有限公司检修公司哈尔滨150090 国网黑龙江省电力有限公司大兴安岭供电公司黑龙江加格达奇165000 国网黑龙江省电力有限公司牡丹江供电公司黑龙江牡丹江157000

高压电力电缆在城市电网输电通道中的占比逐年提高,一旦发生故障将会造成重大损失。阐述一起发生在某500 k V变电站的主变35 k V低压侧电缆终端故障,结合谐波电流在线监测系统计算低压侧系统的电容电流,并结合保护动作时刻的故障录波波... 详细信息

高压电力电缆在城市电网输电通道中的占比逐年提高,一旦发生故障将会造成重大损失。阐述一起发生在某500 k V变电站的主变35 k V低压侧电缆终端故障,结合谐波电流在线监测系统计算低压侧系统的电容电流,并结合保护动作时刻的故障录波波形,对电缆的故障发展过程进行分析。通过解体电缆终端头对故障原因进行定性分析,进而对高压电缆线路的安全运行和故障预防提出建议。

关键词：开关柜电缆终端故障录波

TaGW2L,a GW2-like RING finger E3 ligase,positively regulates heading date in common wheat(Triticum aestivum L.)

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The Crop Journal 2022年第4期10卷 972-979页

作者： Daijing Zhang xiaoxu Zhang Wu Xu Tingting hu Jianhui Ma Youfu Zhang Jian Hou Chenyang Hao Xueyong Zhang Tian Li College of Life Sciences Henan Normal UniversityXinxiang 453007HenanChina Key Laboratory of Crop Gene Resources and Germplasm Enhancement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

RING finger E3 ligases play an important role in regulating plant growth and development by mediating substrate *** this study,we identified TaGW2L,encoding a Grain width and weight2(GW2)-like RING finger E3 ligase,as a novel positive regulator of heading date in wheat(Triticum aestivum L.).TaGW2L exhibited high amino acid sequence similarities with TaGW2 homoeologs,particularly in the conserved RING finger *** analysis indicated that TaGW2L was constitutively expressed in various wheat ***2L showed transactivation activity in yeast and could interact with the ubiquitin-conjugating enzymes E2_(s).An in vitro ubiquitination assay verified that TaGW2L possessed a similar E3 ligase activity to *** of the TaGW2L-7A homoeolog in wheat led to a significantly earlier heading date under both natural conditions and long-day *** analysis revealed that multiple known genes positively regulating wheat heading were significantly upregulated in the TaGW2L-7A-overexpression plants compared with the wild-type ***,our findings shed light on the role of TaGW2L in wheat heading date and provide potential applications of TaGW2L for the adaptation improvement of crops.

关键词： TaGW2L RING finger domain E3 ligase Heading date Triticum aestivum

Decreased Amino Acid Transporter LAT2 Is the Main Determinant of Impaired Protein Utilization During Aging

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Engineering 2024年第11期42卷 88-98页

作者： Rui Song Guang Li Liang Zhao Lili Qiu Xiyu Qin xiaoxu Zhang xiaoxue Liu Jun Zhou Mengxiao hu Liwei Zhang Jiaqi Su Xinjuan Liu Xiaoyu Wang Department of Nutrition and Health China Agricultural UniversityBeijing 100083China Department of Gastroenterology Beijing Chao-yang HospitalCapital Medical UniversityBeijing 100069China Department of Health Sciences and Technology ETH ZurichZürich CH-8092Switzerland

As the global demographic shifts toward an aging population,understanding the efficiency of protein uti-lization in older adults becomes *** study explores the intricate relationship between protein intake and aging,with a focus on precision nutrition for older *** a meta-analysis,we con-firm a decline in protein-utilization capacity in older individuals and examine the different contributions of plant and animal *** experiments involving mice of different ages,older mice exhibited decreases in the biological utilization of four proteins(casein,beef protein,soy protein,and gluten),par-ticularly *** subsequent research,casein was studied as a key protein.A decline in gastric digestion function was observed through peptidomics and the examination of pepsin levels using ***,this decline did not significantly affect the overall protein digestion during the aging *** combined application of targeted amino acid metabolomics identified abnormal absorption of amino acids as the underlying cause of decreased protein utilization during aging,particularly emphasiz-ing a reduction in branched-chain amino acids(BCAAs)in older *** deeper into the proteomics of the intestinal protein digestion and absorption pathway,a reduction of over 60%in large neutral amino acid transporter 2(LAT2)protein expression was observed in both older humans and aged *** reduction in LAT2 protein was found to be a key factor influencing the diminished BCAA ***,our study establishes the significance of amino acid absorption through LAT2 in protein utiliza-tion during aging and offers a new theoretical foundation for improving protein utilization in the older adults.

关键词： Aging Protein utilization Peptide Amino acid Large neutral amino acid transporter 2

The primary assessment of QT/QTc interval prolongation risks of CPRC01 by concentration-QTc model in Chinese healthy subjects

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The primary assessment of QT/QTc interval prolongation risks...

Protective mechanism of Paeoniae Radix Alba against chemical liver injury based on network pharmacology,molecular docking,and in vitro experiments

2018年中国药理学会定量药理学专业委员会学术年会

作者： xiaoxu Wang Dongyang Liu Pei hu Phase I Unit Clinical Pharmacology Research CenterPeking Union Medical College Hospital and Chinese Academy of Medical Sciences

Objectives:A well-designed and conducted QTc assessment based on C-QTc modeling in early phase Ⅰ studies can be an alternative approach to a thorough QT study for some drugs to reliably exclude clinically relevant QTc *** evaluate the effect of CPRC01 on QT/QTc interval in Chinese healthy subjects after a single dose and the dose-effect relationship between the plasma concentration of CPRC01 and QTc interval prolongation,we aimed to develop a C-QTc model of oral CPRC01 in healthy adult ***,to preliminary evaluate the cardiac safety of CPRC01 and provide a basic reference for later clinical ***:The PK data and ECG data was collected from 56 Chinese healthy subjects who received administration of *** mixed effect model as the primary analysis was used to describe the relation between drug concentration and ΔQTcF to exclude a 10 ms QTc prolongation *** preferred dependent variable in the model is the baseline-adjusted and Fridericia heart-rate-corrected QT interval(ΔQTcF).The model parameters were estimated using the restrictive maximum likelihood *** model was evaluated by goodness-of-fit plots,precision of parameters,diagnostic plots,physiological meanings and validated by visual predictive check(VPC) and bootstrap ***,the two-sided 90% confidence interval for model-derived QTcF interval change(ΔΔQTcF) through placebo and baseline double correction was simulated with the model and estimated parameters to analyze the dose-effect relationship between the plasma concentration and QTc interval prolongation,thereby,to ensure the possible maximum concentration that maybe result in QTc interval ***:The model showed that all mean observed values of ΔQTcF were in the band of the two-sided 90% confidence interval of the mean predicted ΔQTcF ***,the solid error bars about the confidence intervals for the mean ΔQTcF interval within each bin were slightly beyond the *** expl

关键词： C-QTc model QT interval prolongation CPRC01 Chinese healthy subjects modeling and simulation

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Journal of Traditional Chinese Medical Sciences 2024年第1期11卷 55-66页

作者： Shuangqiao Liu Xin Liu Sijia Jiang Min Fu Jinxi hu Jiaqi Liu xiaoxu Fan Yingtong Feng Shujing Zhang Jingxia Wang School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing 102488China School of Traditional Chinese Medicine Xinjiang Medical UniversityUrumqi 830017China The Centre for Translational Biology Research Institute of McGill University Health CentreMontr eal H4A 3J1Canada

Objective:To explore and validate the potential targets of Paeoniae Radix Alba(***,Bai Shao)in protecting against chemical liver injury through network pharmacology,molecular docking technology,and in vitro cell ***:Network pharmacology was used to identify the common potential targets of *** and chemical liver *** docking was used to fit the components,which were subsequently verified in vitro.A cell model of hepatic fibrosis was established by activating hepatic stellate cell(HSC)-LX2 cells with 10 ng/mL transforming growth factor-β*** cells were exposed to different concentrations of total glucosides of paeony(TGP),the active substance of ***,and then evaluated using the cell counting kit-8 assay,enzyme-linked immunosorbent assay,and western ***:Analysis through network pharmacology revealed 13 key compounds of ***,and the potential targets for preventing chemical liver injury were IL-6,AKT serine/threonine kinase 1,jun protooncogene,heat shock protein 90 alpha family class A member 1(HSP90AA1),peroxisome proliferator activated receptor gamma(PPARG),PTGS2,and *** Ontology(GO)enrichment analysis indicated the involvement of response to drugs,membrane rafts,and peptide *** Encyclopedia of Genes and Genomes(KEGG)enrichment analysis revealed that the main pathways involved lipid and atherosclerosis and chemical carcinogenesis-receptor *** and albiflorin exhibited strong affinity for HSP90AA1,PTGS2,PPARG,and *** concentrations of TGP can inhibit the expression of COL-I,COL-III,IL-6,TNF-a,IL-1β,HSP-90a,and PTGS2 while increasing the expression of PPAR-γand CASP3 in activated HSC-LX2 ***:*** primarily can regulate targets such as HSP90AA1,PTGS2,PPARG,***,the main active compound of ***,protects against chemical liver injury by reducing the inflammatory response,activating apoptotic proteins,and promoting the apoptosis of activated HSCs.

关键词： Paeoniae Radix Alba Total glycosides of paeony Chemical liver injury Liver fibrosis Network pharmacology Hepatic stellate cells

Pretreatment with Danhong injection protects the brain against ischemia-reperfusion injury

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Neural Regeneration Research 2014年第15期9卷 1453-1459页

作者： Shaoxia Wang Hong Guo Xumei Wang Lijuan Chai Limin hu Tao Zhao Buchang Zhao xiaoxu Tan Feifei Jia Tianjin State Key Laboratory of Modern Chinese Medicine Tianjin University of Traditional Chinese Medicine Tianjin Key Laboratory of Chinese Medicine Pharmacology Tianjin University of Traditional Chinese Medicine Buchang Pharmaceutical Co. Ltd.

Danhong injection （DHI）, a Chinese Materia Medica standardized product extracted from Radix Salviae miltiorrhizae and Flos Carthami tinctorii, is widely used in China for treating acute isch-emic stroke. In the present study, we explored the neuroprotective efficacy of DHI in a rat model of temporary middle cerebral artery ocdusion, and evaluated the potential mechanisms under-lying its effects. Pretreatment with DHI （0.9 and 1.8 mL/kg） resulted in a significantly smaller infarct volume and better neurological scores than pretreatment with saline. Furthermore, DHI significantly reduced the permeability of the blood-brain barrier, increased occludin protein expression and decreased neutrophil infiltration, as well as profoundly suppressing the upreg-ulation of matrix metallopeptidase-9 expression seen in rats that had received vehicle. Matrix metallopeptidase-2 expression was not affected by ischemia or DHI. Moreover, DHI （1.8 mL/kg） administered 3 hours after the onset of ischemia also improved neurological scores and reduced infarct size. Our results indicate that the neuroprotective efficacy of DHI in a rat model of cerebral ischemia-reperfusion injury is mediated by a protective effect on the blood-brain barrier and the reversal of neutrophil infiltration.

关键词： nerve regeneration Danhong injection Radix Salviae Miltiorrhiae Flos Carthami cerebral ischemia-reperfusion neutrophil infiltration matrix metallopeptidase blood-brain barrier NSFC grant neural regeneration