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Transcriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus

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Journal of Oceanology and Limnology 2024年第1期42卷 332-344页

作者： Yuting SONG Maqsood Ahmed SOOMRO Xianzhi DONG guobin hu College of Marine Life Sciences Ocean University of ChinaQingdao 266003China Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdao 266003China Institute of Biophysis Chinese Academy of SciencesBeijing 100101China

Turbot Scophthalmus maximus is an important mariculture fish species with high economic value.However,the bacterial diseases caused by Vibrio anguillarum infection bring huge economic losses to the turbot aquaculture industry.To understand the immune response of the turbot against V.anguillarum infection and to explore novel immune-related genes,the transcriptome analysis of turbot spleen and gills were conducted after V.anguillarum infection.Differentially expressed genes(DEGs)were identified in spleen and gill of the turbot amounted to 17261 and 16436,respectively.A large number of immunerelated DEGs were enriched in cytokine-cytokine receptor interaction signaling pathway,and the others by the kyoto encyclopedia of genes and genomes(KEGG)enrichment.The gene ontology(GO)classification analysis revealed that V.anguillarum infection had the greatest effect on biological processes and cellular components.Twelve immune-related DEGs were identified in the spleen(cstl.1,egfl6,lamb21,v2rx4,calcr,and gpr78a)and gills(ghra,sh3gl2a,cst12,inhbaa,cxcl8,and il-1b)by heat map.The proteinprotein interaction(PPI)networks were constructed to analyze the immune mechanism.The results demonstrate that the maturation and antigen processing of major histocompatibility complex(MHC)class II molecule,and calcitonin-or adrenomedullin-regulated physiological activity were important events in the immunity of turbot against V.anguillarum infection.In the gills,the protein interactions in TGF-βsignaling pathway,production of inflammatory factors,and endocytosis regulation were most significant.Our research laid a foundation for discovering novel immune-related genes and enriching the knowledge of immune mechanisms of turbot against V.anguillarum infection.

关键词： Scophthalmus maximus Vibrio anguillarum transcriptome differentially expressed genes immune mechanism

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Presence of a long nuclear-localization signal sequence in homeodomain transcription factor Nkx 1.2

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Journal of Oceanology and Limnology 2024年第2期42卷 620-626页

作者： Xinyi LI Lihui CHEN Xinyuan WANG Chen SUN Guangdong JI guobin hu Zhenhui LIU College of Marine Life Science and Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdao 266003China Laoshan Laboratory Qingdao 266237China

Homeodomains,a 60-amino acid sequence encoded by 180 nucleotides,are highly conserved DNA-binding motifs that are present in a variety of transcription factors in species ranging from yeast to humans.The NKX proteins belong to the homeodomain(HD)-containing transcription factor family.They play vital roles in the regulation of morphogenesis.NKX1-2 is one member of the NKX subfamily.At present,information about its nuclear localization signal(NLS)sequence is limited.We studied the NLS sequence of zebrafish Nkx1.2 by introducing sequence changes such as deletion,mutation,and truncation,and identified an NLS motif(QNRRTKWKKQ)that is localized at the C-terminus of the homeodomain.Moreover,the deletion of two amino acid residues(RR)in this NLS motif prevents Nkx1.2 from entering the nucleus,indicating that the two amino acids are essential for Nkx1.2 nuclear localization.However,the NLS motif alone is unable to target cytoplasmic protein glutathione S-transferase(GST)to the nucleus.An intact homeodomain is necessary for mediating the complete nuclear transport of cytoplasmic protein.Unlike most nuclear import proteins with short NLS sequences,a long NLS is present in zebrafish Nkx1.2.We also demonstrated that the sequences of homeodomain of NKX1.2 are well conserved among different species.This study is informative to verify the function of the NKX1.2 protein.

关键词： NKX1.2 NKX protein homeodomain nuclear localization signal(NLS) nuclear transport

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Regulating the inner Helmholtz plane structure at the electrolyte-electrode interface for highly reversible aqueous Zn batteries

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Journal of Energy Chemistry 2024年第8期95卷 57-67页

作者： Jianghe Liu Sanlue hu Hexin Guo guobin Zhang Wen Liu Jianwei Zhao Shenhua Song Cuiping Han Baohua Li Shenzhen Key Laboratory on Power Battery Safety and Shenzhen Geim Graphene Center Tsinghua Shenzhen International Graduate School Tsinghua University Shenzhen Key Laboratory of Advanced Materials School of Materials Science and Engineering Harbin Institute of Technology Faculty of Materials Science and Energy Engineering/Institute of Technology for Carbon Neutrality Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen huASUAN Technology Co. Ltd.

The development of aqueous Zn batteries is limited by parasitic water reactions,corrosion,and dendrite growth.To address these challenges,an inner Helmholtz plane（IHP） regulation method is proposed by employing low-cost,non-toxic maltitol as the electrolyte additive.The preferential adsorption behavior of maltitol can expel the water from the inner Helmholtz plane,and thus hinder the immediate contact between Zn metal and H2O.Meanwhile,strong interaction between maltitol and H2O molecules can restrain the activity of H2O.Besides,the "IHP adsorption effect" along with the low LUMO energy level of maltitol-CF3SO3-can promote the in-situ formation of an organic-inorganic complex solid electrolyte interface（SEI） layer.As a result,the hydrogen/oxygen evolution side reaction,corrosion,and dendrites issues are effectively suppressed,thereby leading to highly reversible and dendrite-free Zn plating/stripping.The Zn‖I2battery with hybrid electrolytes also demonstrates high electrochemical performance and ultralong cycling stability,showing a capacity retention of 75% over 20000 charge-discharge cycles at a large current density of 5 A g-1.In addition,the capacity of the device has almost no obvious decay over20000 cycles even at-30℃.This work offers a successful electrolyte regulation strategy via the IHP adsorption effect to design electrolytes for high-performance rechargeable Zn-ion batteries.

关键词： Adsorption effect Dendrite suppression Inner Helmholtz plane SEI layer Zn||I2 battery

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M6PR interacts with the HA2 subunit of influenza A virus to facilitate the fusion of viral and endosomal membranes

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Science China(Life Sciences) 2024年第3期67卷 579-595页

作者： Yuzhen hu Li Jiang Guangwen Wang Yangming Song Zhibo Shan Xuyuan Wang Guohua Deng Jianzhong Shi guobin Tian Xianying Zeng Liling Liu hualan Chen Chengjun Li State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China

Influenza A virus(IAV) commandeers numerous host cellular factors for successful replication. However, very few host factors have been revealed to be involved in the fusion of viral envelope and late endosomal membranes. In this study, we identified cation-dependent mannose-6-phosphate receptor(M6PR) as a crucial host factor for the replication of IAV. We found that siRNA knockdown of M6PR expression significantly reduced the growth titers of different subtypes of IAV, and that the inhibitory effect of M6PR siRNA treatment on IAV growth was overcome by the complement of exogenously expressed M6PR. When A549 cells were treated with siRNA targeting M6PR,the nuclear accumulation of viral nucleoprotein(NP) was dramatically inhibited at early timepoints post-infection, indicating that M6PR engages in the early stage of the IAV replication cycle. By investigating the role of M6PR in the individual entry and post-entry steps of IAV replication, we found that the downregulation of M6PR expression had no effect on attachment, internalization, early endosome trafficking,or late endosome acidification. However, we found that M6PR expression was critical for the fusion of viral envelope and late endosomal membranes. Of note, M6PR interacted with the hemagglutinin(HA) protein of IAV, and further studies showed that the lumenal domain of M6PR and the ectodomain of HA2 mediated the interaction and directly promoted the fusion of the viral and late endosomal membranes,thereby facilitating IAV replication. Together, our findings highlight the importance of the M6PR–HA interaction in the fusion of viral and late endosomal membranes during IAV replication.

关键词： influenza A virus M6PR HA membrane fusion late endosome

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Spatial Heterogeneity of Marine Environment Changes:A Case Study in Laizhou Bay and Its Adjacent Waters,China

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Journal of Ocean University of China 2021年第1期20卷 111-123页

作者： WANG Youxiao YU Ge hu guobin LIU Dahai College of Environmental Science and Engineering Ocean University of ChinaQingdao 266100China Key Laboratory of Marine Environment and Ecology Ministry of EducationQingdao 266100China State Key Laboratory of Resources and Environmental Information System Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China College of Marine Life Sciences Ocean University of ChinaQingdao 266003China First Institute of Oceanography Ministry of Natural ResourcesQingdao 266061China

Temporal and spatial heterogeneity identifications of marine environment and its changes have great significances in marine spatial planning and offshore pollution control.In this study,considering the integration of marine ecological environment and sea-land interaction,we built a spatialized evaluation model named Marine Environment Change Spatial Assessment(MECSA)to quantify the marine environment status and changes.In MECSA,we applied the geospatial model and the pressure-state-response(PSR)model for processing and integrating evaluation indicators.A case study in the Laizhou Bay showed that the marine environment quality was generally in a declining state from 2009 to 2015.In 2015,the Marine Environment Index(MEI)had decreased by 0.1 compared with 2009,although the two years all reached a‘Good'level.The spatial MEI layers of the two years showed a same distribution:the southwestern part was in poorer status,with a fan ring shape gradually getting better to the northeast.The Marine Organisms Response Index(MORI)contributed the most to the MEI.Therefore,future marine environmental assessment and spatial planning should focus on the identification the marine environment with its changes from the perspective of spatial heterogeneity and systemicity.Based on single indicators and comprehensive evaluation results,we can propose spatially targeted policies and recommendations scientifically.

关键词： marine environment quality spatial heterogeneity PSR Laizhou Bay

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深井救援技术与装备研究现状和发展趋势

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工矿自动化 2024年第5期50卷 14-22,35页

作者：文虎侯宗宣郑学召蔡国斌严瑞锦西安科技大学安全科学与工程学院陕西西安710054 国家矿山救援西安研究中心陕西西安710054

深井救援技术是指在深井事故救援过程中对被困人员进行救援各环节涉及的关键技术,主要包括环境侦测技术、生命探测技术、深井快速破拆技术、应急通信网络构建技术及保障深井事故救援顺利进行的其他辅助技术。深井救援装备是指在深井救... 详细信息

深井救援技术是指在深井事故救援过程中对被困人员进行救援各环节涉及的关键技术,主要包括环境侦测技术、生命探测技术、深井快速破拆技术、应急通信网络构建技术及保障深井事故救援顺利进行的其他辅助技术。深井救援装备是指在深井救援技术实施过程中必要的硬件装备和软件平台等。研究深井救援技术和装备对于保障被困人员生命安全、减少事故损失至关重要。分析了深井救援装备及关键技术的研究现状,指出现有的救援技术和装备并不能完全满足复杂多变的环境要求,存在救援装备的通用性与专用性研究不足、救援装备智能化程度有待提升、网络协同能力难以满足救援复杂环境要求、救援装备创新性研究不足等问题。针对上述问题,展望了深井救援装备与技术的发展趋势:①深井救援装备应通过不同救援场景进行专用性和通用性划分,单一装备向多功能性、高可靠性、高机动性发展。②救援装备智能化、精准化、自主决策化,实现智能装备为主、人员为辅的救援模式。③构建急速组网、多模式组网、一体化救援网络平台。④集成TDLAS虽然目前并没有达到救援标准,但其高分辨率、高灵敏度和可集成化在未来将会发挥重要作用,以实现环境监测装备的高集成、轻量化、高效化。

关键词：深井救援救援装备空天地一体智能救援侦测装备挖掘/破拆装备通信装备吊升装备

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Siglec-15与肿瘤免疫关系的研究进展

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中华肿瘤防治杂志 2024年第10期31卷 632-636页

作者：刘子文李涛于源滋付国斌胡锦华山东第一医科大学附属省立医院消化内科山东第一医科大学附属省立医院肿瘤科

目的总结唾液酸结合免疫球蛋白样凝集素-15(Siglec-15)在肿瘤免疫领域的研究进展，以期为肿瘤免疫治疗提供新的理论依据。方法以“Siglec-15、肿瘤、免疫调节、信号通路、免疫治疗”为中文关键词，以“Siglec-15、tumor、immunoregula... 详细信息

目的总结唾液酸结合免疫球蛋白样凝集素-15(Siglec-15)在肿瘤免疫领域的研究进展，以期为肿瘤免疫治疗提供新的理论依据。方法以“Siglec-15、肿瘤、免疫调节、信号通路、免疫治疗”为中文关键词，以“Siglec-15、tumor、immunoregulation、signal pathway、immunotherapy”为英文关键词，系统检索中国知网和PubMed数据库2013-01-01-2023-12-31发表的相关文献。纳入标准：(1)Siglec-15的发现及分子特征；(2)Siglec-15在肿瘤免疫调节中的作用机制及相关信号通路；(3)Siglec-15的免疫治疗前景。排除标准：低质量以及数据陈旧的文献。最终纳入51篇文献进行分析。结果现有研究表明，Siglec-15在多种肿瘤细胞以及肿瘤相关巨噬细胞(TAM)中均高表达。其通过抑制T细胞的活化和增殖来诱导肿瘤的免疫逃逸。此外，Siglec-15还可通过介导特定的分子信号通路调节肿瘤微环境。目前，多种具有抗肿瘤活性的Siglec-15相关药物正在积极开发中，这为肿瘤免疫治疗提供了新的思路。结论近年来，随着程序性死亡受体1(PD-1)及其配体(PD-L1)抗体耐药性问题的出现，Siglec-15可被认为是替代或协同抗PD-1/PD-L1治疗的新兴靶点，有望发挥重要作用。

关键词： Siglec-15 肿瘤免疫调节信号通路免疫治疗

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转录因子HOXC13通过上调PCNA表达促进喉鳞状细胞癌AMC-HN-8细胞的恶性生物学行为

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中国肿瘤生物治疗杂志 2024年第6期31卷 558-565页

作者：兰利利牛云峰胡国斌刘猛徐玉茹王晶田河北医科大学第四医院耳鼻咽喉头颈外科河北石家庄050011 联勤保障部队第九八〇医院病理科河北石家庄050082

目的:本研究旨在探讨转录因子HOXC13在喉鳞状细胞癌(LSCC)中的表达、功能及可能的调控机制。方法:常规培养LSCC细胞,将其分为sh-NC组、sh-HOXC13组、pcDNA3.1-NC组、pcDNA3.1-HOXC13组、pcDNA3.1-PCNA组和sh-HOXC13+pcDNA3.1-PCNA组,用转染试剂将相应核酸和质粒转染各组细胞。用数据库数据分析HOXC13mRNA在LSCC组织中的表达;收集2019年1月至2022年12月在联勤保障部队第九八〇医院耳鼻咽喉头颈外科手术切除的62对LSCC组织及配对的癌旁组织,免疫组织化学法检测中国人LSCC组织中HOXC13蛋白的表达,qPCR检测中国人LSCC组织、癌旁组织以及各组细胞中HOXC13和PCNAmRNA的表达,MTS法检测各组AMC-HN-8细胞的增殖能力,平板克隆实验检测各组AMC-HN-8细胞的克隆形成能力,Transwell小室实验检测各组AMC-HN-8细胞的迁移和侵袭能力,双萤光素酶报告基因实验和染色质免疫沉淀技术(ChIP)验证HOXC13与PCNA之间的结合关系。结果:HOXC13和PCNA在LSCC组织和细胞中均呈高表达(P<0.05或P<0.01)且两者的表达水平呈正相关(P<0.01),HOXC13的表达水平与TNM分期有明显关联(P<0.01)。敲减HOXC13可明显抑制AMC-HN-8细胞的增殖、迁移和侵袭能力(均P<0.01),过表达HOXC13则促进TU686细胞的增殖、迁移和侵袭能力(均P<0.01)。HOXC13可与PCNA启动子区结合并调控其转录。敲低PCNA可部分逆转HOXC13对AMC-HN-8细胞的恶性生物学行为的促进作用(均P<0.01)。结论:HOXC13通过上调PCNA促进LSCC细胞的恶性生物学行为,HOXC13是LSSC临床诊断和治疗的潜在靶点。

关键词：转录因子 HOXC13 喉鳞状细胞癌 AMC-HN-8细胞增殖侵袭迁移 PCNA

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基于无人机多光谱遥感的棉花生长参数和产量估算

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中国农机化学报 2024年第2期45卷 227-234页

作者：赵胜利王国宾胡连槟徐海钰巩道财兰玉彬山东理工大学农业工程与食品科学学院山东淄博255049 山东省农业航空智能装备工程技术研究中心山东淄博255049 山东理工大学生态无人农场研究院山东淄博255049

及时准确地监测棉花长势和产量是精准农业栽培管理的关键。无人机(UAV)平台能够快速获取高时空分辨率的遥感数据,在作物生长参数和产量估算方面显示出巨大的潜力。以山东省滨州市棉花为研究对象,利用安装在无人机上的多光谱相机获取遥... 详细信息

及时准确地监测棉花长势和产量是精准农业栽培管理的关键。无人机(UAV)平台能够快速获取高时空分辨率的遥感数据,在作物生长参数和产量估算方面显示出巨大的潜力。以山东省滨州市棉花为研究对象,利用安装在无人机上的多光谱相机获取遥感影像,分别提取各波段反射率,筛选出8种植被指数,采用多元线性回归(MLR)、随机森林(RF)、人工神经网络(BPNN)3种方法分别构建棉花的株高、叶绿素相对含量、单株产量的估计模型并进行验证。结果表明,基于BPNN的预测模型精度明显优于MLR和RF模型,盛花期与成熟期棉花株高估计模型验证集的R^(2)分别为0.842和0.670;叶绿素相对含量估算模型验证集的R^(2)分别为0.725和0.765;产量估算模型验证集的R^(2)分别为0.860和0.846。为无人机遥感在作物生长参数与产量估算领域中的应用提供理论依据,为进一步优化农业生产管理、科学决策提供参考。

关键词：棉花无人机遥感植被指数株高叶绿素相对含量产量

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超声法制备非金属及其化合物量子点研究进展

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光电工程 2024年第4期51卷 1-16页

作者：张泰玮胡坤李国彬杨奥夏溢坪李学铭唐利斌杨培志云南师范大学能源与环境科学学院可再生能源材料先进技术与制备教育部重点实验室云南昆明650500 昆明物理研究所云南昆明650223 云南省先进光电材料与器件重点实验室云南昆明650223

量子点(Quantum dots,QDs)晶粒直径在1~20 nm之间,是一种零维的纳米材料,具有尺寸可调、激发光谱宽、高量子效率、宽波长范围、光化学稳定性高、不易光解等优异的光学特性。当量子点的尺寸接近或小于激子波尔半径时,原有的连续能带结构... 详细信息

量子点(Quantum dots,QDs)晶粒直径在1~20 nm之间,是一种零维的纳米材料,具有尺寸可调、激发光谱宽、高量子效率、宽波长范围、光化学稳定性高、不易光解等优异的光学特性。当量子点的尺寸接近或小于激子波尔半径时,原有的连续能带结构量子化,其性质也发生显著变化,表现出优异的光电性能。本综述介绍了量子点的制备方法,其中,超声法作为一种常见的“自上而下”方法,因操作简单、对环境友好等优点被广泛使用。首先,概述了利用超声法制备非金属和非金属化合物量子点的机理和表征技术,并分析了分散剂、超声功率和时间等因素对其尺寸及形貌的影响。随后,对超声法制备的非金属和非金属化合物量子点在激光器、太阳能电池等领域的应用展开了讨论,并针对目前研究存在的一些挑战和难题,提出了自己的观点和见解。最后,对超声法制备非金属单质及非金属化合物量子点进行了总结和展望。

关键词：量子点纳米材料超声法非金属单质非金属化合物

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