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数据驱动的混凝土材料-结构一体化设计研究进展

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建筑结构学报 2024年第8期45卷 20-33页

作者：胡张莉刘加平赵羽习赵海涛戚家南王育江韩方玉金鸣东南大学材料科学与工程学院江苏南京211102 浙江大学建筑工程学院浙江杭州310058 河海大学土木与交通学院江苏南京210024 东南大学土木工程学院江苏南京211102 江苏省建筑科学研究院有限公司江苏南京210024

材料-结构一体化设计是指在结构设计中综合考虑材料的性能,而在材料设计中则以结构的需求为出发点,形成材料到结构跨多尺度的设计模式,近年来已成为现代混凝土工程领域的研究重点。基于数据及人工智能算法的混凝土材料、结构设计方法可... 详细信息

材料-结构一体化设计是指在结构设计中综合考虑材料的性能,而在材料设计中则以结构的需求为出发点,形成材料到结构跨多尺度的设计模式,近年来已成为现代混凝土工程领域的研究重点。基于数据及人工智能算法的混凝土材料、结构设计方法可颠覆传统设计依赖经验且需重复试配的范式,实现高效节材及结构创新。梳理了国内外混凝土材料智能设计及材料-结构一体化设计的研究现状,总结了材料层面数据驱动的正向预测及逆向设计的研究范畴和关键问题,梳理了考虑温-湿-约束复杂交互的材料-结构一体化抗裂设计途径与效果,以氯盐侵蚀环境为例分析了混凝土材料-结构耐久性设计方案及提升手段,并以超高性能混凝土为例阐述了混凝土材料-结构高性能设计的方法,提出未来数据驱动的混凝土材料-结构一体化设计框架及生产、设计、制备及结构应用全综合的研究思路,并建议未来应在数据库建设、数据与理论融合及多尺度关联的协同算法等方面开展研究。

关键词：材料-结构一体化设计数据驱动抗裂耐久性高性能

Regenerating Plants from Cryopreserved Adventitious Buds of Haploids in Rice

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Wuhan University Journal of Natural Sciences 1999年第1期4卷 117-119页

作者： Zhang, Zhihong hu, zhangli Wuhan University College of Life Sciences Wuhan China (GRID:grid.49470.3e) (ISNI:***)

A large number of adventitious buds were induced from in vitro cultured young inflorescences of haploids of *** been subcultured on solidified subculture media at 26 ℃ for 7 days ,the adventitious buds were loaded into 1.8 mL plastic cryotubes with cryoprotectant and kept on ice for 45 60 *** cooled at a rate of 1.0℃/min down to -40℃,the samples were kept in liquid *** adventitious buds which have been cryopreserved for about 30 days were thawed rapidly in 38 40℃water and then plated on solidified MS medium containing 3 % sucrose,0.5 mg/L NAA and 2.0 mg/L *** plated, 23% 32 % of adventitious buds resumed growth and 15% 22 % regenerated *** results of this work indicated that the adventitious buds derived from in vitro cultured young inflorescences is a critical factor for the success and subculturing adventitious buds on MS medium containing 3% sucrose and 4% sorbitol or 20% potato extract is essential to the *** effective cryoprotectant is 10 % DMSO(dimethyl sulfoximide)+0.5 mol/L sorbitol.

关键词： Oriza sativa ,haploid adventitious bud,cryopreservation

Heterotrophic bacteria of the Dapeng Bay in the South China Sea: community structure,abundance,and the relationships of culturablity with environmental factors

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Acta Oceanologica Sinica 2010年第4期29卷 88-97页

作者： JIANG Fajun hu zhangli hu Chaoqun South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China Guangxi Academy of Sciences Nanning 530007 China College of Life Sciences Shenzhen University Shenzhen 518060 China

The community composition,abundance and culturablity of heterotrophic bacterioplankton in the Dapeng Bay of the South China Sea were investigated by morphological method,bacterial cultivation and biochemical methods over an annual *** and Pseudomonas were the predominant genera among the Gram-negative *** bacterioplankton abundance fluctuated from 2.07×10 8 to 2.44×10 9 cells/dm 3 ,and culturable bacteria abundance ranged between 0.1×10 6 and 16.07×10 6 CFU/dm 3 .The Culturablity of heterotrophic bacteria ranged between 0.01% and 1.31%.The relationship between bacterial culturablity and the environmental factors was also studied in different sites of the Dapeng *** the bay,a significantly positive relationship was evidenced between bacterial culturability and temperature,meanswhile bacterial culturability was significantly inversely related with *** positive relationship was shown at the central bay while a negative relationship was observed at the bay mouth and there was no significant correlation at top bay between bacterial culturablity and chlorophyll a of sea water.A positive relationship was found between culturability and DIN all the bay,but DIP showed a significantly negative relationship with bacterial culturability only at the mouth of the bay.

关键词： heterotrophic bacteria culturable bacteria culturablity environmental factors

Expression of bkt and bch genes from Haematococcus pluvialis in transgenic Chlamydomonas

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Science China(Life Sciences) 2014年第10期57卷 1028-1033页

作者： ZHENG KaiJing WANG ChaoGang XIAO Ming CHEN Jun LI JianCheng hu zhangli Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science Shenzhen Engineering Laboratory for Marine Algal Biotechnology College of Life Science Shenzhen University

β-carotene ketolase and β-carotene hydroxylase encoded by bkt and bch, respectively, are key enzymes required for astaxanthin biosynthesis in Haematococcu pluvialis 34-1n. Two expression vectors containing cDNA sequences of bkt and bch were constructed and co-transformed into cell-wall-deficient Chlamydomonas reinhardtii CC-849. Transgenic algae were screened on TAP agar plates containing 10 gg mL 1 Zeomycin. PCR-Southern analysis showed that bkt and bch were integrated into the genomes of C. reinhardtii. Transcripts of bkt and bch were further confirmed by RT-PCR-Southern analysis. Compared with the wild type, transgenic algae produced 29.04% and 30.27% more carotenoids and xanthophylls, respectively. Moreover, the transgenic algae could accumulate 34% more astaxanthin than wild type. These results indicate that foreign bkt and bch genes were successfully translated into β-carotene ketolase and β-carotene hydroxylase, which were responsible for catalyzing the biosynthesis of astaxanthin in transgenic algae.

关键词： Chlamydomonas β-carotene ketolase β-carotene hydroxylase carotenoids xanthophylls astaxanthin

Distinct roles of alternative oxidase pathway during the greening process of etiolated algae

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Science China(Life Sciences) 2021年第5期64卷 816-827页

作者： Ying Liu hua Zhang Yan Cui Yanli Zheng hui Chen zhangli hu Qingyu Wu College of Life Sciences and Oceanography Shenzhen UniversityShenzhen 518055China Shenzhen Academy of Environmental Science Shenzhen 518001China Gansu Innovation Center of Microalgae Technology Hexi UniversityZhangye 734000China Key Laboratory of Algal Biology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of the Chinese Academy of Sciences Beijing 100039China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityKaifeng 475004China

The vital function of mitochondrial alternative oxidase(AOX) pathway in optimizing photosynthesis during plant de-etiolation has been well recognized. However, whether and how AOX impacts the chloroplast biogenesis in algal cells remains unclear. In the present study, the role of AOX in regulating the reassembly of chloroplast in algal cells was investigated by treating Auxenochlorella protothecoides with salicylhydroxamic acid(SHAM), the specific inhibitor to AOX, in the heterotrophy to autotrophy transition process. Several lines of evidences including delayed chlorophyll accumulation, lagged reorganization of chloroplast structure, altered PSI/PSII stoichiometry, and declined photosynthetic activities in SHAM treated cells indicated that the impairment in AOX activity dramatically hindered the development of functioning chloroplast in algal cells. Besides, the cellular ROS levels and activities of antioxidant enzymes were increased by SHAM treatment, and the perturbation on the balance of NAD+/NADH and NADP+/NADPH ratios was also observed in A. protothecoides lacking AOX activity, indicating that AOX was essential in promoting ROS scavenging and keeping the redox homeostasis for algal chloroplast development during greening. Overall, our study revealed the essentiality of mitochondrial AOX pathway in sustaining algal photosynthetic performance and provided novel insights into the physiological roles of AOX on the biogenesis of photosynthetic organelle in algae.

关键词： alternative oxidase pathway chloroplast reconstruction greening microalgae reactive oxygen species redox

Directional Breeding of High Itaconic Acid Yielding Strain of <i>Aspergillus terreus</i>with a New Plate Technique

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Advances in Microbiology 2013年第4期3卷 376-381页

作者： Lingyun Wei Jianhua Wang Hezhi Zhou Wenbiao Jin zhangli hu Jiazuan Ni Key Laboratory for Green Chemical Process of Ministry of Education Wuhan Institute of Technology Wuhan China

Itaconic acid is commercially produced by the cultivation of Aspergillus terreus using starch hydrolysate as carbon source. The degree of hydrolysis had a great influence on itaconic acid production which was suitable when corn starch was saccharified at 35 DE. The α-amylase was sufficient to drive the starch hydrolysis to the degree. The agar plate assay with LiCl treatment provided a rapid, simple and unequivocal method for screening large numbers of colonies for itaconic acid producing strains. It was learned by experience that the strains on the plates with thick hyphae and light-colored spores often accompanied high itaconic acid production. A strain, designated Ast165, producing itaconic acid with a high yield, was successfully obtained by directional breeding of metabolic end products resistant strains. The itaconic acid concentration produced by Ast165 was 53.8 g/l from 100 g/l of starch hydrolysate in shake flasks. The conversion rate was 61.3%, which was the highest value found in tests.

关键词： Aspergillus terreus Itaconic Acid Corn Starch Dextrose Equivalent Enzymatic Hydrolysis

Immune reaction characteristics and the mechanism of anergy induced by recombinant enterotoxin a of Staphylococcus aureus

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Journal of Biomedical Science and Engineering 2010年第6期3卷 543-549页

作者： Shang Wu Renli Zhang Dana huang Yijie Geng Shitong Gao Xiaoheng Li zhangli hu 不详

To study immune reactions and the mechanism of anergy induced by recombinant enterotoxin A (rSEA) of Staphylococcus aureus. The gene encoding SEA was cloned from standard strain of S. aureus and high efficiently expressed in E. coli. After immunization with purified rSEA, mice were examined for production of specific antibody, subtype of IgG, cytokine mRNA levels such as IFN-γ, IL-2 secretion and T-cell surface PD-1 expression. Results showed that high levels of specific antibodies were produced in two weeks of primary immunization shot. During this time, humoral immune reactions prevailed (IgG2a/ IgG1 <1). During the early phase, Th1 type cytokine mRNA is expressed at a higher level than Th2 type, indicating cellular immune reaction prevailed. Splen- ocyte IFN-γ secretion was significantly decreased after boosting immunization. The PD-1 expression was detected by a flow cytometry examination in the surface of T- lymphocytes which were induced by rSEA, and the expression of PD-1 molecules increased along with the number of boosting and the time after immunization.

关键词： Staphylococcus Aureus Enterotoxin A Im- mune Respose

细菌活的非可培养(VBNC)状态及其机理研究进展

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应用与环境生物学报 2014年第6期20卷 1124-1131页

作者：邸聪聪胡平胡章立陈辉蓉深圳大学生命科学学院、深圳市海洋生物资源与生态环境重点实验室、深圳市海洋藻类开发与应用工程实验室深圳518060

细菌活的非可培养(Viable but non-culturable,VBNC)状态是指在一定环境胁迫条件下,一些不形成芽孢的细菌仅保留很低代谢活性但无法分裂,失去在培养基上形成菌落能力的状态.细菌的这一状态也可逆转而恢复生长形成菌落,称为复苏.自1982... 详细信息

细菌活的非可培养(Viable but non-culturable,VBNC)状态是指在一定环境胁迫条件下,一些不形成芽孢的细菌仅保留很低代谢活性但无法分裂,失去在培养基上形成菌落能力的状态.细菌的这一状态也可逆转而恢复生长形成菌落,称为复苏.自1982年这一状态被发现以来,已有60余种非芽孢菌被报道可进入VBNC状态,其中包括许多常见致病菌,从而给以菌落培养为基础的常规检测手段带来了挑战.目前VBNC菌研究领域仍以现象报道为主,对其形成条件、生物学特性、遗传机制等尚未完全了解.本综述重点介绍了近期关于VBNC状态与群体感应效应及环境应力机制相关的一些发现和假说,并简述了VBNC状态细菌在细胞形态结构、蛋白表达及毒性方面的变化规律,以及在不同细菌中发现的与复苏过程相关的细胞复苏因子类似物等;揭示了VBNC作为适应机制在不同细菌种群中的普遍性存在及其与细胞间信号传导之间可能存在的密切联系;最后列举了用于VBNC细菌检测的新技术,并提出该领域未来的研究范围应从致病菌扩大到所有环境细菌.

关键词：诱导胁迫复苏促进因子休眠

Expression and function analysis of the metallo-thionein-like (MT-like) gene from Festuca rubra in Chlamydomonas reinhardtii chloroplast

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Science China(Life Sciences) 2008年第12期51卷 1076-1081页

作者： HAN SiHai1,2, hu zhangli1 & LEI AnPing1 1 Institute of Eco-Environment, College of Life Science, Shenzhen University, Shenzhen 518060, China 2 Research Center for Analytical Instrumentation, State Key Lab of Industrial Control Technology, Institute of Cyber-Systems and Control, Zhejiang University, Hangzhou 310058, China Institute of Eco-Environment College of Life Science Shenzhen University Shenzhen China Research Center for Analytical Instrumentation State Key Lab of Industrial Control Technology Institute of Cyber-Systems and Control Zhejiang University Hangzhou China

The cDNA of the metallothionein-like (MT-like) gene from Festuca rubra cv. Merlin was optimized with bias codon of Chlamydomonous reinhardtii chloroplast genome. The optimized MT-like gene was de-livered into C. reinhardtii chloroplast and the transgenic strains expressing MT-like gene was obtained. PCR-Southern blot and RT-PCR-Southern blot analysis demonstrated that the MT-like gene was inte-grated into chloroplast genome of C. reinhardtii and expressed at the transcriptional level. The cad-mium binding capacity of the transgenic C. reinhardtii was determined by hydride generation-atomic fluorescence spectrometry (HG-AFS) and the binding properties were analyzed. Results showed that the transgenic C. reinhardtii expressing the MT-like gene exhibited remarkably higher Cd2+ binding capacity and grew to higher densities at toxic Cd2+ concentrations (40-100 μmol/L) than the wild type strain, and that the IC50 of Cd2+ (3-d treating ) to algal cell growth of transgenic strain was 55.43% higher than that of the wild type strain, indicating that the Cd2+ binding capacity and Cd2+ tolerance of C. reinhardtii was enhanced through the expression of the foreign MT-like gene in chloroplast.

关键词： Chlamydomonas reinhardtii chloroplast metallothionein-like (MT-like) gene cadmium binding properties