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青岛小麦产区丛枝菌根真菌调查

青岛农业大学学报（自然科学版） 2017年第2期34卷 90-94,132页

作者：王维华胡玉金王小珅赵洪海刘润进青岛农业大学菌根生物技术研究所山东青岛266109

丛枝菌根(AM)真菌是重要的种质资源。作者对青岛小麦种植区小麦根区土壤中AM真菌种类、分布、菌根侵染等状况进行调查,旨在初步了解该地区小麦产区AM真菌资源情况,为进一步开展山东省小麦主产区AM真菌群落结构及其生理生态功能奠定基础... 详细信息

丛枝菌根(AM)真菌是重要的种质资源。作者对青岛小麦种植区小麦根区土壤中AM真菌种类、分布、菌根侵染等状况进行调查,旨在初步了解该地区小麦产区AM真菌资源情况,为进一步开展山东省小麦主产区AM真菌群落结构及其生理生态功能奠定基础。自青岛城阳区和胶州小麦主产区采集小麦(济麦22)根围样品,共分离鉴定AM真菌4属17种,其中无梗囊霉属(Acaulospra)4种、管柄囊霉属(Funneliformis)1种、球囊霉属(Glomus)11种和巨孢囊霉属(Gigaspora)1种。其中,Acaulospra bireticulata、Acaulospra denticulate、Glomus geosporum、Rhizophagus intraradices和Gigaspora margarita是青岛小麦产区土壤中AM真菌的优势种。该区小麦根系AM侵染率在13%~30%之间,2月份侵染率最高,4~5月份则最低;AM真菌孢子数量4月份最多。本研究初步明确了山东青岛地区小麦根区土壤中AM真菌发育与分布情况,为进一步探究山东麦区麦根区土壤中AM真菌分布规律,筛选促进小麦生长的AM真菌提供了条件和技术基础。

关键词：丛枝菌根真菌小麦田间调查青岛优势菌种

Main flow channel index in porous sand reservoirs and its application

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Petroleum Exploration and Development 2020年第5期47卷 1055-1061页

作者： LI Xizhe LUO Ruilan hu Yong XU Xuan JIAO Chunyan GUO Zhenhua WAN yujin LIU Xiaohua LI Yang PetroChina Research Institute of Petroleum Exploration&Development Beijing 100083China

Based on well test interpretation,production performance analysis,overburden permeability and porosity test,gas-water core flooding test and high-pressure mercury injection,a quantitative correlation has been built of in-situ effective permeability with routine permeability and water saturation,and the ranges of Main Flow Channel Index(MFCI)are determined for different permeability levels in porous sand gas reservoirs.A new method to evaluate the in-situ effective permeability of porous sand reservoir and a correlation chart of reserves producing degree and main flow channel index are *** results reveal that the main flow channel index of porous sand gas reservoirs has close correlation with routine matrix permeability and water *** lower the routine matrix permeability and the higher the water saturation,the lower the MFCI *** the routine matrix permeability is greater than 5.0×10-3,the MFCI is generally greater than *** the routine matrix permeability is from 1.0×10-3 to 5.0×10-3,the MFCI is mainly between 0.2 and *** the routine matrix permeability is less than 1.0×10-3,the MFCI is less than *** evaluation method of in-situ effective permeability can be used to evaluate newly discovered or not tested porous sand gas reservoirs quickly and identify whether there is tight sand *** correlation chart of reserves producing degree and main flow channel index can provide basis for recoverable reserves evaluation and well infilling,and provide technical support for formulation of reasonable technical policy of gas reservoir.

关键词： porous sand reservoir main flow channel index influence factor evaluation method producing reserves

Genome-wide alternative splicing variation and its potential contribution to maize immature-ear heterosis

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The Crop Journal 2021年第2期9卷 476-486页

作者： Xiaojiao hu Hongwu Wang Kun Li Xiaogang Liu Zhifang Liu yujin Wu Shuqiang Li Changling huang National Engineer Laboratory of Crop Molecular Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Heterosis is a well-known phenomenon widely applied in *** studies have suggested that differential gene and protein expression between hybrids and their parents play important roles in *** splicing(AS)is an essential posttranscriptional mechanism that can greatly affect the transcriptome and proteome diversity in ***,genome-wide AS divergence in hybrids compared to their parents and its potential contribution to heterosis have not been comprehensively *** report the direct profiling of the AS landscape using RNA sequencing data from immature ears of the maize hybrid ZD808 and its parents NG5 and *** results revealed a large number of significant differential AS(DAS)events in ZD808 relative to its parents,which can be further classified into parental-dominant and novel DAS ***-dominant,especially NG5-dominant,events were prevalent in the hybrid,accounting for 42%of all analyzed DAS *** enrichment analysis revealed that the NG5-dominant AS events were involved mainly in regulating the expression of genes associated with carbon/nitrogen metabolism and cell division processes and contributed greatly to maize ear *** ZD808,CL11,and NG5,32.5%of DAS contained or lacked binding sites of at least one annotated maize microRNA(miRNA)and may be involved in miRNA-mediated posttranscriptional *** regulation was the predominant contributor to AS variation and participates in many important biological processes associated with immature ear *** study provides a comprehensive view of genome-wide alternative splicing variation in a maize hybrid.

关键词： Maize Heterosis Dominant alternative splicing Cis-regulation

Genetic analysis and QTL mapping of stalk cell wall components and digestibility in maize recombinant inbred lines from B73 × By804

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The Crop Journal 2020年第1期8卷 132-139页

作者： Qi Wang Kun Li Xiaojiao hu huimin Shi Zhifang Liu yujin Wu Hongwu Wang Changling huang National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijingChina National Engineering Laboratory for Crop Molecular Breeding BeijingChina

The cell wall composition and structure of the maize stalk directly affects its digestibility and in turn its feed *** studies of stem quality have focused mostly on common maize germplasm,and few studies have focused on high-oil cultivars with high grain and straw *** of the genetic basis of cell wall composition and digestibility of maize stalk using high-oil maize is desirable for improving maize forage *** the present study,a high-oil inbred line(By804)was crossed as male parent with the maize inbred line B73 to construct a population of 188 recombinant inbred lines(RILs).The phenotypes of six cell-wall-related traits were recorded,and QTL analysis was performed with a genetic map constructed with SNP *** traits were significantly correlated with one another and showed high broad-sense *** 20 QTLs mapped,the QTL associated with each trait explained 10.0%–41.1%of phenotypic *** half of the QTL each explained over 10%of the phenotypic *** results provide a theoretical basis for improving maize forage quality by marker-assisted selection.

关键词： Maize QTL Cell wall Digestibility Recombinant inbred lines

QTL analysis of ear leaf traits in maize(Zea mays L.)under different planting densities

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The Crop Journal 2017年第5期5卷 387-395页

作者： Hongwu Wang Qianjin Liang Kun Li Xiaojiao hu yujin Wu hui Wang Zhifang Liu Changling huang Institute of Crop Science Chinese Academy of Agricultural Sciences

Modem maize varieties have become more productive than ever, owing largely to increased tolerance of high plant density. However, the genetics of ear leaf traits under different densities remains poorly understood. In this study, Zhongdan 909 recombinant inbred lines(RILs) derived from a cross between Z58 and HD568 were genotyped for 3072 single-nucleotide polymorphisms(SNPs), and phenotyped for leaf length(LL), leaf width(LW), and leaf angle(LA) of the uppermost ear leaf under three planting densities(52,500,67,500, and 82,500 plants ha-1, respectively). A genetic map was then constructed using1358 high-quality SNPs. The total length of the linkage map was 1985.2 cM and the average interval between adjacent markers 1.46 cM. With increasing density, LL and LW decreased from 63.68 to 63.02 cm and from 8.56 to 8.21 cm, respectively, while LA increased from19.42° to 19.66°. All three traits had high heritabilities, of 0.75, 0.78, and 0.84, *** inclusive composite interval mapping, 23, 25, and 17 quantitative trait loci(QTL) were detected for LL, LW, and LA, respectively. Of these, 35 were simultaneously detected under two or three plant densities, while 30 were detected under only one. Sixty-five individual QTL explained 2.41% to 16.53% of phenotypic variation, while eight accounted for >10%.These findings will help us understand the genetic basis of leaf traits in maize as well as the response of maize to increased plant density.

关键词： Maize Quantitative trait loci Leaf traits Density treatment

Optimization of ultrasonic-assisted freezing of Penaeus chinensis by response surface methodology

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Food Quality and Safety 2021年第2期5卷 143-151页

作者：应宇斌向迎春刘佳琳陈欣胡玲萍 yujin Li 胡亚芹 College of Biosystems Engineering and Food Science National-Local Joint Engineering Laboratory of Intelligent Food Technology and EquipmentZhejiang Key Laboratory for Agro-Food ProcessingIntegrated Research Base of Southern Fruit and Vegetable Preservation TechnologyZhejiang International Scientific and Technological Cooperation Base of Health Food Manufacturing and Quality ControlNingbo Research InstituteFuli Institute of Food ScienceZhejiang UniversityHangzhouChina College of Food Science and Technology Hainan Tropical Ocean UniversitySanyaChina College of Food Science and Engineering Ocean University of ChinaQingdaoChina

Objectives:Optimization of ultrasonic-assisted freezing of Penaeus chinensis by response surface methodology was studied in order to(1)obtain frozen Penaeus chinensis of high quality and(2)provide practical guidance for the application of ultrasonic-assisted freezing in Penaeus *** and Methods:Three independent and major variables were selected,including initial ultrasonic temperature(℃),ultrasonic power(W)and ultrasonic time(s on/2 s off).On the basis of one-factor experiments,17 groups of experiments were established by response surface methodology according to Box-Behnken *** multiple regression analysis the experimental data were fitted into a second-order polynomial equation,which was tested by proper statistical ***:The optimal ultrasonic conditions were as follows:initial ultrasonic temperature 0℃,ultrasonic power 180 W,ultrasonic time 5 son/2 s *** the optimization conditions,the time of passing through maximum ice crystal generation zone was 105.500 s,which was very close to the predictive passage time of 101.541 ***:Initial ultrasonic temperature,ultrasonic time and ultrasonic power played an important role in the process of ultrasonic-assisted freezing of Penaeus *** surface methodology was used to optimize the three factors in ultrasonic-assisted freezing,which could greatly shorten the time of passing through the maximum ice crystal generation zone and maintain the tissue structure of Penaeus chinensis well.

关键词： Ultrasonic-assisted freezing Penaeus chinensis response surface methodology time of passing through the maximum ice crystal generation zone optimization

雷公藤甲素通过调控NLRP3通路抑制LPS诱导的BV2小胶质细胞炎性反应

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山西大同大学学报（自然科学版） 2024年第1期40卷 75-80页

作者：穆秉桃李玉璐刘淇源孙肖乐胡芬琦曹佳蕾杨凤君李佩佩毕宇锦王泽涛张慧宇山西大同大学脑科学研究所/分子细胞免疫学大同市重点实验室山西大同037009 山西大同大学医学院山西大同037009 山西大同大学中医药健康服务学院山西大同037009

目的基于炎症小体(NLRP3)通路探讨雷公藤甲素(Triptolide TP)抑制小胶质细胞炎性反应的机制。方法脂多糖(LPS)刺激BV2小胶质细胞促细胞炎症损伤,建立体外模型,观察TP对LPS刺激的小胶质细胞的影响。BV2小胶质细胞分为对照组、LPS诱导的... 详细信息

目的基于炎症小体(NLRP3)通路探讨雷公藤甲素(Triptolide TP)抑制小胶质细胞炎性反应的机制。方法脂多糖(LPS)刺激BV2小胶质细胞促细胞炎症损伤,建立体外模型,观察TP对LPS刺激的小胶质细胞的影响。BV2小胶质细胞分为对照组、LPS诱导的模型组(1 mg/L LPS)、TP组(1 nmol/L TP+1 mg/L LPS)。对照组不做处理,其它组药物作用24 h,镜下观察药物组细胞发生的形态变化并和对照组比较;细胞上清液用Griess法测定一氧化氮(NO)的释放量;免疫荧光染色和Western blot法检测各组细胞间炎症小体蛋白3(NLRP3)、凋亡相关斑点样蛋白(ASC)、半胱氨酸天冬氨酸蛋白酶(caspase-1)和白细胞介素-18(IL-18)的水平差别。结果LPS诱导BV2细胞炎性活化,形态呈阿米巴样,降低细胞活性,促进NO的释放。TP作用后降低NO水平,使NLRP3炎性小体激活受到抑制,NLRP3、ASC、caspase-1和IL-18表达水平低下。结论TP对小胶质细胞炎性反应有抑制作用,发挥了TP的抗炎作用,抑制了NLRP3炎性小体的活化和表达。

关键词：雷公藤甲素脂多糖 BV2细胞 NLRP3炎症小体炎症反应

2.7 Ga Monzogranite on the Songnen Massif and Its Geological Implications

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Acta Geologica Sinica(English Edition) 2018年第3期92卷 1265-1266页

作者： WU Xinwei ZHANG Chao ZHANG yujin GUO Wei ZHANG Chao CUI Tianri YANG Yajun hu Jianfei SONG Wanbing Shenyang Institute of Geology and Mineral Resources China Geological Survey Shenyang 110034 China College of Earth Sciences Jilin University Changchun 130061 China Resources and Environmental Engineering of Shandong University of Technology Zibo 255049Shandong China

Objective The formation and evolution of Songnen massif has always been a hot topic,and the presence of Precambrian basement on the Songnen massif is still controversial:(1)Lacking of Pre-Paleozoic dating results,the Precambrian basement on Songnen massif does not extend largely according to the isotopic dating results of core from basement,(2)the existence of gneiss from deep drill holes

关键词： the Songnen Massif Its Geological Implications Ga Monzogranite

Internal friction behaviors of Ni-Mn-In magnetic shape memory alloy with two-step structural transformation

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Progress in Natural Science:Materials International 2017年第3期27卷 356-361页

作者： Zhen-ni Zhou Liang Yang Jun-ge Wang Tianfan Jin yujin huang Jun Li Qiaodan hu Jian-guo Li School of Materials Science and Engineering Shanghai Jiao Tong University Collaborative Innovation Center for Advanced Ship and deep-Sea Exploration Shanghai Jiao Tong University

The internal friction（IF） behaviors of dual-phase Ni;Mn;In;alloy with two-step structural transformation were investigated by dynamic mechanical *** IF peak for the martensite transformation（MT） is an asymmetric shoulder rather than those sharp peaks for other shape memory *** intermartensitic transformation（IMT） peak has the maximum IF *** the heating rate increases,the height of the IMT peak increases and its position is shifted to higher *** comparison with the IMT peak,the MT peak is independent on the heating *** starting temperatures of the IMT peak are strongly dependent on frequency,while the MT peak is weakly ***,the heights of both the MT and IMT peak rapidly decrease with increasing the *** work also throws new light on their structural transformation mechanisms.

关键词： Magnetic shape memory alloys Martensite transformation Ni-Mn-In Internal friction Dynamic mechanical analysis