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HPLC-APCI-MS Determination of Free Fatty Acids in Tibet Folk Medicine Lomatogonium rotatum with Fluorescence Detection and Mass Spectrometry Identification

HPLC-APCI-MS Determination of Free Fatty Acids in Tibet Folk...

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首届中国中西部地区色谱学术交流会暨仪器展览会

作者： Yulin Li, Xian’en Zhao, Jinmao You, Yourui Suo, fengzu hu, Guichen Chen ( Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, 810001, PR china department of Chemistry, qufu normal university, qufu, 273165, PR china Graduate university of Chinese Academy of Sciences, Beijing, 100039, PR china)

A simple and sensitive method for the determination of free fatty acids (FFAs) using acridone-9-ethyl-p-toluenesulfonate (AETS) as fluorescence derivatization reagent by high-performance liquid chromatography (HPLC) has been developed. Free fatty acid derivatives were separated on Eclipse XDB-Cs column with a good baseline resolution and detected with the fluorescence which excitation and emission wavelengths of derivatives were set at λex=404 and λem=440nm, respectively. Identification of 19 fatty acid derivatives was carried out by online post-column mass spectrometry with atmospheric pressure chemical ionization (APCI) source under positive-ion detection mode. 19 FFAs from the extract of Lomatogonium rotation are sensitively determined. The results indicate that the plant Lomatogonium rotatum enriches an abundance of FFAs and FFAs of higher contents mainly focus on that of even carbon atoms C14, C16 and C18. The validation of the method including linearity, repeatability, and detection limits was examined. Most linear correlation coefficients for fatty acid derivatives are >0.9989, and detection limits (at signal-to-noise of 3:1) are 12.3-43.7 fmol. The relative standard deviations (RSDs) of the peak areas and retention times for 19 FFAs standards are 2.24 % and 0.45 %, respectively. The established method is rapid and reproducible for the separation determination of FFAs from the extract of Lomatogonium rotatum with satisfactory results.

关键词： HPLC-APCI-MS FFAs AETS fluorescence detection derivatization Lomatogonium rotatum

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Fluorescence of Rhodamine B doped SiO2 gels by the sol-gel method

Fluorescence of Rhodamine B doped SiO2 gels by the sol-gel m...

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第九届全国发光学术会议

作者： Ji hui-hui,Li Qing-shan,Wang Qing-tao,Dong Yan-feng (qufu normal university,qufu,Shandong,273165)

The sol-gel process is an inorganic polymerization procedure based on the hydrolysis and condensationreactions of metal qlkoxides. It has gained importance with respect to scientific and application *** this method, a great variety of materials,e.g.,oxidic semiconductors,glasses and even conducting filmscan be obtained.

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IKIP downregulates THBS1/FAK signaling to suppress migration and invasion by glioblastoma cells

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Oncology Research 2024年第7期32卷 1173-1184页

作者： ZHAOYING Zhu YANJIA hu feng YE HAIBO TENG GUOLIANG YOU YUNhuI ZENG MENG TIAN JIANGUO XU JIN LI ZHIYONG LIU HAO LIU NIANDONG ZHENG department of Neurosurgery The Affiliated Hospital of Southwestern Medical UniversityLuzhouChina department of Neurosurgery Sichuan University West China HospitalChengduChina

Background:Inhibitor of NF-κB kinase-interacting protein(IKIP)is known to promote proliferation of glioblastoma(GBM)cells,but how it affects migration and invasion by those cells is ***:We compared levels of IKIP between glioma tissues and normal brain tissue in clinical samples and public *** examined the effects of IKIP overexpression and knockdown on the migration and invasion of GBM using transwell and wound healing assays,and we compared the transcriptomes under these different conditions to identify the molecular mechanisms ***:Based on data from our clinical samples and from public databases,IKIP was overexpressed in GBM tumors,and its expression level correlated inversely with *** overexpression in GBM cells inhibited migration and invasion in transwell and wound healing assays,whereas IKIP knockdown exerted the opposite *** overexpression in GBM cells that were injected into mouse brain promoted tumor growth but inhibited tumor invasion of surrounding *** effects of IKIP were associated with downregulation of THBS1 mRNA and concomitant inhibition of THBS1/FAK ***:IKIP inhibits THBS1/FAK signaling to suppress migration and invasion of GBM cells.

关键词： Inhibitor of NF-κB kinase-interacting protein(IKIP) Glioblastoma(GBM) Migration Thrombospondin 1(THBS1) FAK signaling

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Caliper-based precise positioning of the target(CALIPPOT)for transcranial magnetic stimulation without neuronavigation system

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General Psychiatry 2024年第2期37卷 311-314页

作者： Yunsong hu Rong Zeng Juan Yue Qiu Ge Hongxiao Wang Zijian feng Jue Wang Yufeng Zang Center for Cognition and Brain Disorders The Affiliated Hospital of Hangzhou Normal UniversityHangzhouZhejiangChina Zhejiang Key Laboratory for Research in Assessment of Cognitive Impairments HangzhouZhejiangChina Institutes of Psychological Sciences Hangzhou Normal UniversityHangzhouZhejiangChina Transcranial Magnetic Stimulation Center Deqing Hospital of Hangzhou Normal UniversityDeqingZhejiangChina Institute of Sports Medicine and Health Chengdu Sport UniversityChengduSichuanChina department of Neurology The Affiliated Hospital of Hangzhou Normal UniversityHangzhouZhejiangChina

To the editor:Transcranial magnetic stimulation(TMS)is a non-invasive brain modulation *** important usage of TMS is the transient interruption of cognitive brain function(also named virtual lesion)for investigating precisely where and when a specific cortical region contributes to a specific cognitive function.1 A more important usage of TMS is the treatment of brain disorders by repetitive TMS(rTMS).

关键词： stimulation technique. precise

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不同生境蚶形无齿蚌的形态观察

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四川动物 2004年第4期23卷 322-324,F003页

作者：舒凤月欧阳珊曲阜师范大学生命科学学院山东曲阜273165 南昌大学生物科学工程系

对不同生境下蚶形无齿蚌壳的形态、育儿囊的类型和结构以及钩介幼虫等进行了比较研究。结果表明 ,在不同生境下 ,蚶形无齿蚌个体大小有很大差异 ,壳具有高度的可塑性 ;育儿囊由两片外鳃构成 ,为外鳃类的同生型 ,钩介幼虫在育儿囊内呈散... 详细信息

对不同生境下蚶形无齿蚌壳的形态、育儿囊的类型和结构以及钩介幼虫等进行了比较研究。结果表明 ,在不同生境下 ,蚶形无齿蚌个体大小有很大差异 ,壳具有高度的可塑性 ;育儿囊由两片外鳃构成 ,为外鳃类的同生型 ,钩介幼虫在育儿囊内呈散乱状态存在 ;在不同区域 ,钩介幼虫的大小不同 ,但其壳高与壳长的比例却是一致的 ,且不同生境钩介幼虫的超微结构相同。

关键词：不同生境育儿形态观察超微结构可塑性状态幼虫无齿蚌壳长壳高

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CT examination, clinical situation and experimental characteristics of infants with intracranial hemorrhage induced by delayed vitamin K deficiency

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Neural Regeneration Research 2006年第9期1卷 801-805页

作者： Zhiqing Lin1, feng Fang1, Min Chen2, Guoxiang Cai3 1department of Pediatrics, Tongji Hospital Affiliated to Tongji Medical College, huazhong university of Science and Technology, Wuhan 430030, hubei Province, china 2department of Pediatrics, 3department of Radiation Medicine, Zhongshan Hospital Affiliated to Xiamen university, Xiamen 361004, Fujian Province, china department of Pediatrics Tongji Hospital Huazhong University of Science and Technology Wuhan 430030 Hubei Province China department of pediatrics Zhongshan Hospital Xiamen University Xiamen 361004 Fujian Province China department of Radiation Medicine Zhongshan Hospital Xiamen University Xiamen 361004 Fujian Province China

BACKGROUND: Delayed vitamin K deficiency is characterized by acute onset, severe illness and high fatality rate. 33%-50% survivors accompany with other various nervous system sequelas. Therefore, diagnosis and treatment of intracranial hemorrhage in time become a key factor for improving healing rate and reducing fatality rate and incidence of sequela. OBJECTIVE: To investigate the clinical situation, experimental characteristics, CT examination and terminative characteristics of infants with intracranial hemorrhage induced by delayed vitamin K deficiency. DESIGN: Case analysis. SETTING: department of Pediatrics, Zhongshan Hospital Affiliated to Xiamen university. PARTICIPANTS: A total of 17 infants with intracranial hemorrhage induced by delayed vitamin K deficiency aged 1-3 months including 11 boys and 6 girls were selected from department of Pediatrics, Zhongshan Hospital Affiliated to Xiamen university from January 1994 to December 2005. All infants had drowsiness, rejective milk, spiting milk, gaze of both eyes, tic, coma, full anterior fontanelle, high muscular tension and cerebral hernia, etc. Experimental examination demonstrated that infants had anemia at various degrees; prothrombin time and partial thromboplastin time were prolonged; platelet count was normal. CT examination indicated that screenages of subarachnoid hemorrhage, subdural hematoma, cerebral parenchyma hemorrhage and intraventricular hemorrage were changed. Hemorrhage was stopped by the application of vitamin K. All patients provided informed consent. METHODS: ① Clinical situation and physical sign of infants were observed after hospitalization and scanned with rapid spiral CT scanning system. The thickness and average space of layers were 8-10 mm and the scanning time was 5 s with window width of 30-80 hu and window position of 28-35 hu. ② After hospitalization, four items of blood coagulation was measured with Futura meter and biochemical indexes of blood, such as serum calcium, serum alka

关键词： clinical situation and experimental characteristics of infants with intracranial hemorrhage induced by delayed vitamin K deficiency CT examination

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英汉语言文化中礼貌原则的差异

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山东行政学院山东省经济管理干部学院学报 2004年第1期 113-114页

作者：胡玮曲阜师范大学山东曲阜273165

英汉语言文化中礼貌原则的差异,实质上就是中西方在总的文化背景方面差异的具体体现。以中西方文化背景的差异为主线,从隐私语、称呼语、谦辞与客套等方面进行对比研究,对于提高学生的跨文化交际能力大有裨益。

关键词：英汉语言文化礼貌原则文化差异

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Establishment of a humanized ST6GAL1 mouse model for influenza research

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Animal Models and Experimental Medicine 2024年第3期7卷 337-346页

作者： Lyu Chao Han feng Gao Qian Lv Limin Lu Ziwei Lu Shuangshuang Li Xiaoyan hu Yuechao Yang Mengjie Zhao Yingze Liu Jun Lu Xuancheng Duo Shuguang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Chinese Center for Disease Control and PreventionBeijingChina Institute of Zoology Chinese Academy of SciencesBeijingChina NHC Key Laboratory of Biosafety National Key Laboratory of Intelligent Tracking and Forecasting for Infectious DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Zhejiang Provincial Key Laboratory of Medical Genetics Key Laboratory of Laboratory MedicineMinistry of EducationSchool of Laboratory Medicine and Life SciencesWenzhou Medical UniversityWenzhouChina

Background:This study aimed to construct and characterize a humanized influenza mouse model expressing ***:humanized fragments,consisting of the endothelial cell-specific K18 promoter,human ST6GAL1-encoding gene,and luciferase gene,were microinjected into the fertilized eggs of *** manipulated embryos were transferred into the oviducts of pseudopregnant female *** offspring were identified using *** exhibiting elevated expression of the hST6GAL1 gene were selectively bred for propagation,and in vivo analysis was performed for *** of the humanized gene was tested by performing immunohistochemical(IHC)*** and biochemical analyses using the whole blood and serum of humanized hST6GAL1 mice were ***:Successful integration of the human ST6GAL1 gene into the mouse genome led to the overexpression of human SiaT *** mice were identified as carrying copies of the humanized gene,and the in vivo analysis indicated that hST6GAL1gene expression in positive mice mirrored influenza virus infection *** IHC results revealed that hST6GAL1 was expressed in the lungs of humanized ***,the hematologic and biochemical parameters of the positive mice were within the normal ***:A humanized influenza mouse model expressing the hST6GAL1 gene was successfully established and characterized.

关键词： hST6GAL1 humanized mice influenza animal model

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Kinematic Study on Motoman HP6 Arc Welding Robot Based on Unigraphics

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Transactions of Tianjin university 2011年第3期17卷 199-202页

作者：冯胜强胡绳荪申俊琦 Tianjin Key Laboratory of Advanced Joining Technology School of Material Science and Engineering Tianjin University Ningbo Branch of china Academy of Ordnance Science

The links of Motoman HP6 arc welding robot are considered as an open kinematic chain which consists of a series of rotational joints through concatenation. One end of the open chain is fixed to the base or the earth, and the other end which is free fastens the end executor to complete various duties. Each link of this arc welding robot has four kinds of Denavit-Hartenberg parameters: common normal length between two adjacent links, angle of two adjacent joints, distance between the crossing of common normal length and two joints axes, and angle of two adjacent links. The displacement relation between each link of the Motoman HP6 arc welding robot is introduced, and the kinematic positive-going solution and the kinematic passive-going solution are calculated.

关键词： arc welding robot link Denavit-Hartenberg parameter kinematic positive-going solution kinematicpassive-going solution off-line programming

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对性别助长假设的初步验证:在场观众的性别影响作业效绩吗?

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心理科学 2004年第6期27卷 1400-1403页

作者：李朝旭莫雷 feng Wenlu 曲阜师范大学教育科学学院心理学系曲阜273165 华南师范大学心理应用研究中心广州510631 department of Management Studies School of Business Centennial College Ontario M1K 5E9

为了检验金盛华于 1 989年提出的“性别助长假设” ,采用现场实验探讨观众魅力对性别助长效应的干涉作用。 32个中专学生 (男女各半 )在 4种观众条件下测试BAH时间。结果表明 ,观众条件对女性被试的作业效绩无影响 ;而高魅力异性观众的... 详细信息

为了检验金盛华于 1 989年提出的“性别助长假设” ,采用现场实验探讨观众魅力对性别助长效应的干涉作用。 32个中专学生 (男女各半 )在 4种观众条件下测试BAH时间。结果表明 ,观众条件对女性被试的作业效绩无影响 ;而高魅力异性观众的在场能助长男性被试的效绩 ,而低魅力的异性观众在场会削弱其效绩。因此 ,性别助长效应受到诸多因素 (如被试的性别、观众的魅力 )的干涉 ,男性易出现助长现象 ,且高魅力的异性观众才对作业效绩具有助长作用。

关键词：性别助长假设在场观众作业效绩社会助长自我展示印象管理异性效应观众效应

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