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Elimination of grapevine fleck virus and grapevine rupestris stem pitting-associated virus from Vitis vinifera 87-1 by ribavirin combined with thermotherapy

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Journal of Integrative Agriculture 2021年第9期20卷 2463-2470页

作者： hu guo-jun DONG Ya-feng ZHANG Zun-ping FAN Xu-dong REN Fan Research Institute of Pomology Chinese Academy of Agricultural SciencesXingcheng 125100P.R.China

Vitis vinifera 87-1 plants infected by grapevine fleck virus(GFkV) and grapevine rupestris stem pitting-associated virus(GRSPaV) were used as the plant materials for virus elimination treatment. This study evaluated the effects of ribavirin at different concentrations(15 and 25 μg mL^(–1);R15 and R25, respectively), thermotherapy(37°C;T), and the combination of ribavirin and thermotherapy(R15+T and R25+T) on eliminating viruses from grapevine plants in vitro. Both R15 and R25 had phytotoxic effects and weakened plant growth. Thermotherapy positively affected the growth of grapevine plants. Plant height was significantly greater in T, R15+T, and R25+T than in CK, R15 and R25. The proportion of dead plants after T, R15+T, and R25+T was 51.4, 11.4, and 8.6%, respectively. The survival rates of regenerated plants after all treatments were >68.0%. Ribavirin concentration and treatment time were related to the regeneration of shoot tips and elimination efficiencies of the two viruses. The survival rates of plants after R15+T for 30, 40, and 50 days were 97.3, 90.7, and 74.4%, respectively. The elimination rates of GRSPaV from plants in the three time quantum were 55.6, 84.6, and 93.8%, respectively. The elimination rate of GFkV was 23.9% higher in R25(35/44) than in R15(25/45), and that of GRSPaV was 7.0% higher in R25 than in R15. The combination of thermotherapy and chemotherapy was found to have a positive effect on the eradication of GFkV and GRSPaV, and R25+T for 50 days was able to completely eliminate the two viruses from in vitro grapevines.

关键词： in vitro grapevine grapevine fleck virus(GFk V) grapevine rupestris stem pitting-associated virus(GRSPaV) chemotherapy thermotherapy virus elimination

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Molecular characterization of Apple necrotic mosaic virus identified in crabapple(Malus spp.) tree of China

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Journal of Integrative Agriculture 2019年第3期18卷 698-701页

作者： hu guo-jun DONG Ya-feng ZHANG Zun-ping FAN Xu-dong REN Fang Research Institute of Pomology Chinese Academy of Agricultural Sciences

Apple necrotic mosaic virus(ApNMV) was identified in crabapple trees with mosaic symptoms from Zaozhuang, Shandong Province, China, by reverse transcription polymerase chain reaction(RT-PCR) analysis. The complete nucleotide sequences of one isolate from crabapple(ApNMV-Hai) and two isolates from apple(ApNMV-hua and-Qu) were determined. The sizes of genomic RNA1, 2 and 3 of the three isolates differed from those of the previously reported isolate ApNMV-P126 from Japanese apple, especially RNA3. Compared with the nucleotide(nt) sequence of RNA3 in isolate P126, those in the Hai and Qu isolates were 7 and 33 nt shorter, respectively, and that of isolate hua was 7 nt longer. Alignment analyses showed that these differences in size were mainly due to differences in the lengths of the 5′ untranslated region(UTR) and the UTR region between the ORFs encoding the movement protein and the coat protein. In the phylogenetic trees constructed using the full genomic sequences of RNA1, 2 and 3, the isolate Hai clustered into a group with the isolate Qu in the RNA1 tree, but formed an individual branch in the RNA2 and 3 trees. Three recombination events were identified in the nucleotide sequences of RNA1 and 2 among the isolates ApNMV-Hai,-hua, and-Qu. This is the first report of the full genome sequence of ApNMV in crabapple.

关键词： crabapple mosaic symptom Apple necrotic mosaic virus molecular characterization

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Prevalence and genetic diversity of grapevine fabavirus isolates from different grapevine cultivars and regions in China

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Journal of Integrative Agriculture 2020年第3期19卷 768-774页

作者： FAN Xu-dong ZHANG meng-yan ZHANG Zun-ping REN Fang hu guo-jun DONG Ya-feng National Center for Eliminating Viruses from Deciduous Fruit Tree Research Institute of PomologyChinese Academy of Agricultural Sciences

A total of 288 grapevine samples of 61 different grapevine cultivars,collected from 22 provinces and regions,were analyzed by reverse transcription-PCR(RT-PCR) for the presence of grapevine fabavirus(GFabV).PCR detection results showed the incidences of GFabV were 12.8%(30/235) and 48.1%(25/52) in the asymptomatic and symptomatic vines,*** genetic diversity of GFabV isolates was analyzed based on partial nucleotide and encoded amino acid sequences of the RNA1 and RNA2 polyprotein *** analyses of the RNA1 and RNA2 gene sequences divided the GFabV isolates into five well-defined *** 1,2,and 4 comprised only Chinese *** article represents the first report for the prevalence and genetic diversity of GFabV in grapevines grown in China.

关键词： GFabV RT-PCR polyprotein gene phylogenetic analysis

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A sensitive SYBR Green RT-qPCR method for grapevine virus E and its application for virus detection in different grapevine sample types

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Journal of Integrative Agriculture 2020年第7期19卷 1834-1841页

作者： REN Fang ZHANG Zun-ping FAN Xu-dong hu guo-jun ZHANG Meng-yan DONG Ya-feng National Center for Eliminating Viruses from Deciduous Fruit Trees Research Institute of PomologyChinese Academy of Agricultural SciencesXingcheng 125100P.R.China

To develop a rapid and high-sensitivity method for detection of grapevine virus E(GVE),a SYBR Green based real-time fluorescence quantitative RT-PCR method(RT-qPCR)was *** method could be used to detect GVE specifically,and the sensitivity was about 100 times greater than conventional *** excellent linear correlation(R=0.997)and a high amplification efficiency(E=97.5%)were obtained from the standard curve of this *** tests revealed that the coefficients of variation in the intra-and inter-assay results were 0.31-1.03%and 0.82--262%,respectively,indicating a good *** RT-qPCR method could be used to detect GVE in a wide range of grapevine sample *** detection rates of RT-qPCR for nearly all sample types from different positions and seasons were higher than conventional *** detection rates in spring,summer,autumn and winter increased *** in autumn and winter were best for detection,and the detection rates of most samples were 80-100%,which were 10 to 40%higher than conventional *** general,old petioles and branches were the best tissues for GVE *** detection rates of these samples in each season were all 100%,which were 20 to 40%higher than conventional *** second highest rates were in the old leaf,with detection rates for RT-qPCR of 80-100%in all seasons,which were 20 to 40%higher than conventional *** could be difficultly detected in young leaves by conventional RT-PCR,and the detection rates were only 0-50%,while by RT-qPCR the rates could increase to 0--80%.A total of 33 out of 363 samples(belonging to 68 cultivars)from 20 regions in China were detected to be positive by RT-qPCR(9.1%),which was more than twice the rate of the conventional RT-PCR(3.9%).

关键词： grapevine grapevine virus E detection RT-qPCR conventional RT-PCR

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Effect of pre-culture on virus elimination from in vitro apple by thermotherapy coupled with shoot tip culture

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Journal of Integrative Agriculture 2018年第9期17卷 2015-2023页

作者： hu guo-jun DONG Ya-feng ZHANG Zun-ping FAN Xu-dong REN Fang LI Zheng-nan Research Institute of Pomology Chinese Academy of Agricultural Sciences

We evaluated the role of pre-culture on survival rate of in vitro apple plants treated by thermotherapy. Two apple cultivars, Malusxdomestica cv. Pink Lady and huafu, were used in the experiment and both have widely grown in China and infected with Apple chlorotic leafspot virus （ACLSV） and Apple stem grooving virus （ASGV）. Results in growth and virus titer of apple plants did not exhibit clear trends during five different periods of pre-culture. Whilst, pre-culture increased the survival rate of the two cultivars during thermotherapy. The survival rate of plants pre-cultured for 13 d （P-13d） was 14 and 51% higher than that of P-ld plants for Pink Lady and huafu, respectively. Moreover, pre-culture positively influenced regeneration of huafu plants. The average survival rate of plants regenerated from P-ld and P-4d was 20% lower than that regenerated from P-7d, P-10d, and P-13d. The efficiency of virus eradication was determined by reverse-transcription PCR with two primer pairs for each virus, and the detection results showed that pre-culture scarcely affected apple virus elimination. Despite the fact that the two viruses were hardly detected at 5 d of thermotherapy, no virus-free plants were found in the two cultivars of regenerated apple plantlets after 30-d treatment.

关键词： apple in vitro culture pre-culture thermotherapy virus elimination

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P-tuned FeN2 binuclear sites for boosted CO2 electro-reduction

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Journal of Energy Chemistry 2025年第2期101卷 816-824页

作者： Cao guo Sanshuang Gao jun Li Menglin Zhou Abdukader Abdukayum Qingquan Kong Yingtang Zhou Guangzhi hu School of Materials and Energy Institute for Ecological Research and Pollution Control of Plateau Lakes School of Ecology and Environmental Science Yunnan University Xinjiang Key Laboratory of Novel Functional Materials Chemistry College of Chemistry and Environmental Sciences Kashi University School of Mechanical Engineering Chengdu University National Engineering Research Center for Marine Aquaculture Marine Science and Technology College Zhejiang Ocean University

The recycling of CO2through electrochemical processes offers a promising solution for alleviating the greenhouse effect;however,the activation of CO2and desorption of*CO in electrocatalytic CO2reduction（ECR） frequently encounter high energy barriers and competitive hydrogen evolution reactions（HERs）,which are urgent problems that need to be *** this study,a catalyst(P100-Fe-N/C) with homogeneous P-tuned FeN2binuclear sites（N2PFe-FePN2） was successfully synthesised,demonstrating satisfactory performance in the ECR to CO.P100-Fe-N/C attains a peak FECOof 98.01% and a normalized TOF of664.7 h-1at-0.7 VRHE,surpassing the performance of the Fe binuclear catalyst without P and singleatoms *** the MEA cell,a FECOexceeding 90% can still be *** functional theory analysis indicates that the asymmetric coordination configuration induced by the incorporation of P facilitates a reduction in the system's *** modulation of P results in the d-band centre of the catalyst being positioned closer to the Fermi level,which facilitates the interaction of the catalyst with CO2,allowing more electrons to be injected into the CO2molecule at the Fe binuclear sites and inhibiting the *** P-tuned FeN2binuclear sites effectively lower the*CO desorption barrier.

关键词： CO2 reduction reaction DFT calculation Diatomic catalyst Electrocatalysis Heteroatom doping

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Effect of Nb-V microalloying on the hot deformation behavior of medium Mn steels

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 360-368页

作者： Yumeng Wang Qinyi guo Bin hu Haiwen Luo School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China

The influence of Nb-V microalloying on the hot deformation behavior and microstructures of medium Mn steel(MMS)was investigated by uniaxial hot compression *** establishing the constitutive equations for simulating the measured flow curves,we successfully constructed deformation activation energy(Q)maps and processing maps for identifying the region of flow *** concluded the following consequences of Nb-V alloying for MMS.(i)The critical strain increases and the increment diminishes with the increasing deformation temperature,suggesting that NbC precipitates more efficiently retard dynamic recrystallization(DRX)in MMS compared with solute Nb.(ii)The deformation activation energy of MMS is significantly increased and even higher than that of some reported high Mn steels,suggesting that its ability to retard DRX is greater than that of the high Mn content.(iii)The hot workability of MMS is improved by narrowing the hot processing window for the unstable flow stress,in which fine recrystallized and coarse unrecrystallized grains are present.

关键词： medium manganese steel niobium-vanadium microalloying flow behavior dynamic recrystallization hot workability

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Expression of Overlapping PreS1 Fragment Recombinant Proteins for the Determination of Immunogenic Domains in HBsAg PreS1 Region

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Acta Biochimica et Biophysica Sinica 2004年第6期36卷 397-404页

作者： Wei-guo hu, jun WEI&, Xin-Xiu YANG, Heng-Chuan XIA, Feng LI, and Zu-Chuan ZHANG* Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,the Chinese Academy of Sciences, Shanghai 200031, China Key Laboratory of Proteomics Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of SciencesShanghai 200031 China

In this study, eight preS1 fragments overlapped in preS1 (21–119) region of HBV adr subtype, i.e. preS1 (21–47), preS1 (34–59), preS1 (48–70), preS1 (60–85), preS1 (71–94), preS1 (86–109), preS1 (95–119) and preS1 (21–119), were cloned by PCR, and expressed as GST fusion proteins. These GST- preS1 fusion proteins were highly expressed in soluble form in E. coli, and about 50 to 90 mg soluble fusion proteins were purified from 1 L culture. Using these fusion proteins, the immunogenic domains in preS1 (21– 119) region were identified by Western blot analysis and competitive ELISA. The results showed that the immunogenic domains mainly existed in preS1 (21–59) in N-terminus and preS1 (95–109) in C-terminus, and more importantly, a major immunogenic domain preS1 (34–59), which has much stronger immunogenicity, was identified. It was also supported by the predictions of secondary structure and immunological property in the preS1 (21–119) region. The results here would be helpful for the design of new vaccines against HBV.

关键词： HBV preS1 overlapping fragment immunogenic domain

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Gradient age-precipitation behavior induced by gradient nano-grain in Mg-Gd-Ag-Zr alloy

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Journal of Iron and Steel Research International 2025年第1期32卷 19-24页

作者： Bang-jun Li Jia-peng Sun Jing Han guo-song Wu College of Materials Science and Engineering Hohai UniversityNanjing 210024JiangsuChina School of Mechanical and Electrical Engineering China University of Mining and TechnologyXuzhou 221116JiangsuChina

Gradient nano-grained structure is frequently engineered into metallic materials,including Mg alloys,to achieve superior combination of strength and ***,the influence of this microstructural feature on aging precipitation behavior remains inadequately *** this study,the age-precipitation behavior of a gradient nano-grained Mg-Gd-Ag-Zr alloy prepared via ultrasonic severe surface rolling was *** result indicates that the aging precipitation behavior exhibits a depth-dependent variation within this *** the nano-grained surface layer,hierarchicalβnano-precipitates are predominant,while at greater depths,the precipitates consist ofβnanoparticles located at grain boundaries,along with intragranularβ′andγ″*** formation ofβnano-precipitates deviates from the conventional precipitation behavior observed in Mg-Gd-Ag alloys,and is attributed to the high density of defects induced by the surface nano-grained *** finding advances our understanding of the precipitation behavior in the alloys with heterogeneous microstructure.

关键词： Mg alloy Gradient Precipitation Aging behavior Microstructure

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Cubature Kalman Fusion Filtering Under Amplify-and-Forward Relays With Randomly Varying Channel Parameters

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IEEE/CAA Journal of Automatica Sinica 2025年第2期12卷 356-368页

作者： Jiaxing Li Zidong Wang jun hu Hongli Dong Hongjian Liu the Department of Applied Mathematics Harbin University of Science and Technology IEEE the Department of Computer Science Brunel University London the School of Automation Harbin University of Science and Technology the Artificial Intelligence Energy Research Institute Northeast Petroleum University the Heilongjiang Provincial Key Laboratory of Networking and Intelligent Control Northeast Petroleum University the National Key Laboratory of Continental Shale Oil of China Northeast Petroleum University the Key Laboratory of Advanced Perception and Intelligent Control of High-End Equipment Ministry of Education Anhui Polytechnic University the School of Mathematics and Physics Anhui Polytechnic University

In this paper, the problem of cubature Kalman fusion filtering(CKFF) is addressed for multi-sensor systems under amplify-and-forward(AaF) relays. For the purpose of facilitating data transmission, AaF relays are utilized to regulate signal communication between sensors and filters. Here, the randomly varying channel parameters are represented by a set of stochastic variables whose occurring probabilities are permitted to exhibit bounded uncertainty. Employing the spherical-radial cubature principle, a local filter under AaF relays is initially constructed. This construction ensures and minimizes an upper bound of the filtering error covariance by designing an appropriate filter gain. Subsequently, the local filters are fused through the application of the covariance intersection fusion rule. Furthermore, the uniform boundedness of the filtering error covariance's upper bound is investigated through establishing certain sufficient conditions. The effectiveness of the proposed CKFF scheme is ultimately validated via a simulation experiment concentrating on a three-phase induction machine.

关键词： Amplify-and-forward (AaF) relays covariance intersection fusion cubature Kalman filtering multi-sensor systems uniform boundedness

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