检索结果-南通市图书馆

Screening Helicobacter pylori genes induced during infection of mouse stomachs

世界胃肠病学杂志：英文版 2012年第32期18卷 4323-4334页

作者： Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei gu Hong Sang Emmalena gregory-Bryson william g Wood Yisheng Ni Kimberly Smith Sharon H Jackson william g Coleman Laboratory of Biochemistry and genetics National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda MD 20892 United States Laboratory of Host Defenses National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda MD 20892 United States

AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(***) as it relates to its survival in the ***:In vivo expression technology(IVET) systems are used to identify microbial virulence *** modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and *** novel IVET systems were developed by the fusion of random Sau3A DNA fragments of *** and a tandem-reporter system of chloramphenicol acetyltransferase and ***,each vector contains a kanamycin resistance *** used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that *** expresses in *** expression studies were conducted by infecting RAW 264.7 cells with *** was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced ***:In this study,we have identified 31 in vivo induced(ivi) genes in the initial *** 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse *** factors,vacA and cagA,were found in this screen and are known to play important roles in *** infection,colonization and *** detection validates the efficacy of these screening *** of the identified ivi genes have already been implicated to play an important role in the pathogenesis of *** and other bacterial pathogens such as Escherichia coli and Vibrio *** profiles of allivi genes were confirmed by real time PCR analysis of *** RNA isolated from *** infected RAW 264.7 *** compared the expression profile of *** and RAW 264.7 coculture with that of *** *** ge

关键词：幽门螺旋杆菌基因表达感染过程诱导基因胃炎小鼠筛选卡那霉素抗性基因

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Screw dislocation structure and mobility in body centered cubic Fe predicted by a gaussian Approximation Potential

引用

npj Computational Materials 2018年第1期4卷 90-96页

作者： Francesco Maresca Daniele Dragoni gábor Csányi Nicola Marzari william A.Curtin Laboratory for Multiscale Mechanics Modeling(LAMMM) Institute of Mechanical EngineeringÉcole Polytechnique Fédérale de LausanneLausanne CH-1015Switzerland Theory and Simulation of Materials(THEOS) and National Centre for Computational Design and Discovery of Novel Materials(NCCR MARVEL)École Polytechnique Fédérale de LausanneLausanne CH-1015Switzerland Dipartimento di Scienza dei Materiali Universitàdi Milano-BicoccaVia R.Cozzi 55Milan I-20125Italy Department of Engineering University of CambridgeTrumpington StreetCambridge CB21PZUK

The plastic flow behavior of bcc transition metals up to moderate temperatures is dominated by the thermally activated glide of screw dislocations,which in turn is determined by the atomic-scale screw dislocation core structure and the associated kink-pair nucleation mechanism for *** complex plasticity phenomena requires the simulation of many atoms and interacting dislocations and *** sizes are beyond the scope of first-principles methods and thus require empirical interatomic *** for the technological important case of bcc Fe,existing empirical interatomic potentials yield spurious ***,the structure and motion of the screw dislocations in Fe are studied using a new gaussian Approximation Potential(gAP)for bcc Fe,which has been shown to reproduce the potential energy surface predicted by density-functional theory(DFT)and many associated *** Fe gAP predicts a compact,non-degenerate core structure,a single-hump Peierls potential,and glide on{110},consistent with DFT *** thermally activated motion at finite temperatures occurs by the expected kink-pair nucleation and propagation *** stress-dependent enthalpy barrier for screw motion,computed using the nudgedelastic-band method,follows closely a form predicted by standard theories with a zero-stress barrier of~1 eV,close to the experimental value of 0.84 eV,and a Peierls stress of~2 gPa consistent with DFT predictions of the Peierls potential.

关键词： structure dislocation cubic

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fast and Stable Zinc Anode‑Based Electrochromic Displays Enabled by Bimetallically Doped Vanadate and Aqueous Zn^(2+)/Na^(+)Hybrid Electrolytes

引用

Nano-Micro Letters 2023年第12期15卷 231-241页

作者： Zhaoyang Song Bin Wang Wu Zhang Qianqian Zhu Abdulhakem YElezzabi Linhua Liu william WYu Haizeng Li College of Chemistry and Chemical Engineering Qingdao UniversityQingdao 266071People’s Republic of China Optics and Thermal Radiation Research Center Institute of Frontier and Interdisciplinary ScienceShandong UniversityQingdao 266237People’s Republic of China School of Chemistry and Chemical Engineering Shandong UniversityJinan 250100People’s Republic of China Ultrafast Optics and Nanophotonics Laboratory Department of Electrical and Computer EngineeringUniversity of AlbertaEdmontonAB T6G 2V4Canada

Vanadates are a class of the most promising electrochromic materials for displays as their multicolor ***,the slow switching times and vanadate dissolution issues of recently reported vanadates significantly hinder their diverse practical ***,novel strategies are developed to design electrochemically stable vanadates having rapid switching *** show that the interlayer spacing is greatly broadened by introducing sodium and lanthanum ions into V_(3)O_(8)interlayers,which facilitates the transportation of cations and enhances the electrochemical *** addition,a hybrid Zn^(2+)/Na^(+)electrolyte is designed to inhibit vanadate dissolution while significantly accelerating electrochemical *** a result,our electrochromic displays yield the most rapid switching times in comparison with any reported Zn-vanadate electrochromic *** is envisioned that stable vanadate-based electrochromic displays having video speed switching are appearing on the near horizon.

关键词： Vanadates Hybrid electrolytes Displays Electrochromic

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Broadband usage for rural communities in the North Island of Aotearoa New Zealand

引用

Intelligent and Converged Networks 2022年第3期3卷 244-259页

作者： Ming Xiang william Liu Edmund Lai Jairo gutierrez Luca Chiaraviglio Jinsong Wu Control Science and Engineering South China University of TechnologyGuangzhou 510300Chinaand also with the South China Sea Institute of Planning and Environmental ResearchMinistry of Natural ResourcesGuangzhou 510300China Department of Computer Science and Software Engineering School of EngineeringComputer and Mathematical SciencesAuckland University of TechnologyAuckland 1010New Zealand Department of Electronic Engineering University of Rome“Tor Vergata”Rome 00133Italy Consorzio Nazionale Interuniversitario per le Telecomunicazioni(CNIT) Parma 43124Italy Department of Electrical Engineering Universidad de ChileSantiago 92101Chile

New Zealand government’s Rural Broadband Initiative(RBI)aims to invest 400 million New Zealand dollar to provide 99%of New Zealanders with access to 50 Mbps peak broadband speed,with the remaining 1%at 10 Mbps by *** 2017,stage 1 of the RBI has been completed,and research is needed to find out the level of usage,proficiency,and productivity achieved by ***,a survey was carried out to learn whether the rural residents and their communities are making good use of the new fast broadband *** survey collected data from 217 rural residents from North Island,and the results indicate that about half of the interview respondents are satisfied with the new broadband speed and the reliability which is offered by RBI stage ***,there is about 28%of the respondents disagreed with *** majority of the Internet uses for rural residents are information searching,reading news,online entertainment,and online *** that,only a small proportion of respondents know how to utilize the Internet in their work/business and benefit/profit from it,e.g.,using cloud technology capabilities and online marketing campaigns,*** ***,we argue that information and communications technologies(ICT)adoption is not only the availability of the infrastructure but also the beneficial outcomes of Internet *** other words,Internet skills in-depth training and education need to catch up with the infrastructure deployment,which is useful to fuel the digital productivity and inclusion for booming rural *** survey data-driven findings presented in this paper could serve as a reference to inform government policymakers and those who wish to create,invest,and take actions to speed up the economic and social growth of rural communities in Aotearoa New Zealand through the Internet while shifting from the Internet speed and traffic volume-driven to a more effective Internet connectivity and value-added driven rural economy.

关键词： data driven approach data-supported decision and policy making digital exclusion information and communications technologies(ICT)adoption effective connectivity rural broadband usage rural economic development

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Isolated Igg4-associated autoimmune hepatitis or the first manifestation of Igg4-related disease?

引用

Hepatobiliary & Pancreatic Diseases International 2023年第4期22卷 415-417页

作者： Carmela Cosentino Daniel Clayton-Chubb Catriona McLean Stuart K Roberts william Kemp Department of gastroenterology and Hepatology Alfred HealthMelbourne 3004VICAustralia Central Clinical School Monash UniversityMelbourne 3004VICAustralia Department of Anatomical Pathology Alfred HealthMelbourne 3004VICAustralia

To the Editor:Immunoglobulin g4(Igg4)-associated autoimmune hepatitis(Igg4-AIH)is a novel and rare disease entity,characterized by sig-nificant infiltration of Igg4-expressing plasma cells in the *** classification of of Igg4-AIH as a subtype of AIH or an early manifestation of Igg4-related disease(Igg4-RD)remains ***,we discuss an interesting clinical vignette of Igg4-AIH in a gentleman with no significant past medical history,who presented with undifferentiated symptoms and elevated aminotransferases.

关键词： Igg4 AIH autoimmune

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

User-friendly finite element design of main entries, barrier pillars,and bleeder entries

引用

International Journal of Mining Science and Technology 2018年第1期28卷 3-10页

作者： william g.Pariseau Mark K.Larson Heather E.Lawson Douglas R.Tesarik Department of Mining Engineering University of Utah Spokane Mining Research Division NIOSH

This contribution describes development and application of a user-friendly finite element program,UT3PC, to address three important problems in underground coal mine design:(1) safety of main entries,(2) barrier pillar size needed for entry protection, and(3) safety of bleeder entries during the advance of an adjacent longwall *** the finite element method is by far the most popular engineering design tool of the digital age, widespread use by the mining community has been impeded by the relatively high cost of and the need for lengthy specialized training in numerical *** of UT3PC overcomes these impediments in three easy ***, a material properties file is prepared for the considered ***, mesh generation is automatic through an interactive process.A third and last step is simply execution of the *** using data from several western coal mines illustrate the ease of using the application for analysis of main entries, barrier pillars, and bleeder entry safety.

关键词： Finite element Coal Mine layout design Main entries Barrier pillars Bleeder entries

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Refractory very early-onset inflammatory bowel disease associated with cytosolic isoleucyl-tRNA synthetase deficiency:A case report

引用

World Journal of gastroenterology 2020年第15期26卷 1841-1846页

作者： Andrew Fagbemi william g Newman Stuart g Tangye Stephen M Hughes Edmund Cheesman Peter D Arkwright Department of Paediatric gastroenterology Royal Manchester Children’s HospitalManchester M199WLUnited Kingdom Department of Medical genetics Manchester University NHS Foundation TrustManchester M139WLUnited Kingdom Evolution and genomic Sciences University of ManchesterManchester M139WLUnited Kingdom garvan Institute of Medical Research DarlinghurstNSW 2010Australia St Vincent’s Clinical School Faculty of MedicineUNSW AustraliaSydneyNSW 2010Australia Department of Paediatric Allergy and Immunology Royal Manchester Children’s HospitalManchester M139WLUnited Kingdom Department of Paediatric Histopathology St Mary’s HospitalManchester M139WLUnited Kingdom Lydia Becker Institute of Immunology and Inflammation University of ManchesterManchester M139WLUnited Kingdom

BACKgROUND Aminoacyl tRNA synthetases/ligases(ARSs)are highly conserved enzymes involved in attaching amino acids to tRNA promoting protein *** deficiencies of ARSs localized to the mitochondria classically present with neuropathology,the clinical features of cytosolic ARS deficiencies are more *** have previously been associated with neonatal hepatitis,but never with early-onset inflammatory bowel *** SUMMARY A nine-year-old Bangladeshi boy presented with neonatal liver failure and deranged clotting,transaminitis and *** parents were first *** older brothers and a sister were *** patient suffered from loose stools from early infancy which became more troublesome and persistent from five years old with ten bloody motions a *** endoscopies showed persistent pancolitis,which was refractory to mesalazine,corticosteroids,azathioprine,sirolimus and anti-TNF(adalimumab)therapy,but has improved recently with subcutaneous *** genome Sequencing revealed a novel pathogenic missense variant(c.290A>g)in the cytosolic isoleucyl-tRNA synthetase gene,leading to an amino acid substitution(***97gly).Pathogenic variants in other genes associated with inflammatory bowel disease(IBD)(ADAM17,EgFR,FOXP3,IL10RA,IL10RB,IL21R,NCF4,STAT3)were *** assays demonstrated markedly elevated IL-2,IL-5,IL-13,IL-9 and IL-10 by the patient’s CD4+T-cells,while IL-17A,IL-17F,IFNβwere lower,and TNFαnot significantly different when compared to healthy *** This case report provides evidence that recessive mutations in cytosolic isoleucyltRNA synthetase are a novel monogenic cause of IBD,which should be considered,particularly in infants and children with a history of neonatal hepatitis and very early-onset IBD poorly responsive to treatment.

关键词： Inflammatory bowel disease Hepatitis gene Cytosolic isoleucine tRNA synthase

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Traditional Chinese medicine extraction method by ethanol delivers drug-like molecules

引用

Chinese Journal of Natural Medicines 2019年第9期17卷 713-720页

作者： william g.Miao Chunping Tang Yang Ye Ronald J.Quinn Yunjiang Feng griffith Institute of Drug Discovery(gRIDD) Griffith UniversityQueensland 4111Australia Shanghai Institute of Materia Medica Chinese Academy of SciencesShanghai 201203China

Traditional Chinese Medicine(TCM)is an important reservoir for bioactive natural *** extraction methods by water decoction and wine tincture are an integral part of TCM and essential for their widely acknowledged *** this study,we selected 6 common TCMs that are rich in chemistry to investigate whether the TCM extraction methods deliver molecules with drug-like physical chemical *** TCM herbal materials were extracted by water,95%ethanol,and sequential hexane,d ichloromethane and *** extracts were analyzed by HPLC and^1H *** on one of the extracts yielded 32 compounds,their physical chemical properties were analyzed by Instant *** results showed that ethanol extraction,which mimics TCM wine tincture,delivered compounds with physical chemical properties compliant to Lipinski’s rule of 5.

关键词： Extraction Traditional Chinese medicine(TCM) Natural products Lipinski’s Ro5Physicochemical properties

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

How tightly is the nuclear symmetry energy constrained by a unitary Fermi gas?

引用

Nuclear Science and Techniques 2017年第12期28卷 248-254页

作者： Nai-Bo Zhang Bao-Jun Cai Bao-An Li william g.Newton Jun Xu Department of Physics and Astronomy Texas A&M University-Commerce Shandong Provincial Key Laboratory of Optical Astronomy and Solar-Terrestrial Environment Institute of Space Sciences Shandong University Department of Physics Shanghai University Department of Physics and Astronomy Shanghai Key Laboratory for Particle Physics and Cosmology Shanghai Jiao Tong University Shanghai Institute of Applied Physics Chinese Academy of Sciences

We examine critically how tightly the density dependence of nuclear symmetry energy E_(sym)(q) is constrained by the universal equation of state of the unitary Fermi gas EUg(q) considering currently known uncertainties of higher order parameters describing the density dependence of the equation of state of isospin asymmetric nuclear matter. We found that E_(Ug)(q) does provide a useful lower boundary for the E_(sym)(q). However, it doesnot tightly constrain the correlation between the magnitude E_(sym)(q_0) and slope L unless the curvature K_(sym)of the symmetry energy at saturation density q_0 is more precisely known. The large uncertainty in the skewness parameters affects the E_(sym)(q_0) versus L correlation by the same almost as significantly as the uncertainty in K_(sym).

关键词： Symmetry energy Unitary gas Equation of state Nuclear matter

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Fundamentals of business / 1st ed. 1st ed.

引用

2005年

作者： Nickels william g.

来源：畅想之星图书光盘版（南... 评论

在线全文

畅想之星图书光盘版（南通市图书馆镜像）

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：