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Choline suppresses hepatocellular carcinoma progression by attenuating AMPK/mTOR-mediated autophagy via choline transporter SLC5A7 activation

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Hepatobiliary Surgery and Nutrition 2024年第3期13卷 393-411,I0001,I0002页

作者： Chen Wang Zhao-Yan Liu Wen-Ge Huang Zhi-jun Yang Qiu-Ye Lan Ai-Ping Fang Meng-jun hou Xiao-Lin Luo Yao-jun Zhang Si Chen Hui-Lian Zhu Department of Nutrition School of Public HealthSun Yat-sen UniversityGuangzhouChina Guangdong Provincial Key Laboratory of Food Nutrition and HealthSchool of Public HealthSun Yat-sen UniversityGuangzhouChina Center of Experimental Animals Sun Yat-sen UniversityGuangzhouChina Experimental and Teaching Center for Public Health School of Public HealthSun Yat-sen UniversityGuangzhouChina Department of Hepatobiliary Oncology Sun Yat-sen University Cancer CenterGuangzhouChina

Background:Hepatocellular carcinoma(HCC)is one of the leading causes of cancer-associated *** evidence suggests that autophagy plays a critical role in HCC tumorigenesis,metastasis,and *** is an essential nutrient related to prolonged survival and reduced risk of ***,it remains unclear whether this phenomenon is mediated by ***:Two HCC cell lines(HUH-7 and Hep3B)were used in the present *** growth was evaluated by cell counting kit 8(CCK-8),colony formation,and in vivo mouse xenografts *** motility was calculated by wound healing and transwell *** were measured by transmission electron microscope(TEM),and autophagy flux was detected by mRFP-GFP-labeled LC3 *** mRNA level of genes was measured by quantitative real-time polymerase chain reaction(qRT-PCR).The protein levels were detected by Western blotting(WB).Results:We found that choline inhibited the proliferation,migration,and invasion of HCC cells by downregulating autophagy in vitro and in *** expression of the solute carrier family 5 member 7(SLC5A7),a specific choline transporter,correlated with better HCC *** further discovered that choline could promote SLC5A7 expression,upregulate cytoplasm p53 expression to impair the AMPK/mTOR pathway,and attenuate ***,we found that choline acted synergistically with sorafenib to attenuate HCC development in vitro and in ***:Our findings provide novel insights into choline-mediated autophagy in HCC,providing the foothold for its future application in HCC treatment.

关键词： Hepatocellular carcinoma(HCC) autophagy choline solute carrier family 5 member 7(SLC5A7)

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Sphingosine 1-phosphate acts as an activator for the porcine Gpr3 of constitutively active G protein-coupled receptors

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第7期13卷 555-566页

作者： Bao-le ZHANG Ye LI Jian-hua DING Fu-lu DONG Yan-jun hou Bao-chun JIANG Fang-xiong SHI Yin-xue XU College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095China

We cloned the complete coding sequences of porcine Gpr3, Gpr6, and Gpr12 genes. Further, on the basis of their high levels of sequence similarity, these genes are identified as a subfamily of G protein-coupled receptors. These putative protein sequences also showed high sequence identity with other mammalian orthologs, including several highly conserved motifs. A wide expression of the Gpr3 gene in pigs was observed through tissue distribution analysis by reverse transcriptase-polymerase chain reaction （RT-PCR） and real-time PCR, specially in the brain, pituitary, fat, liver and oocyte, where its strong expression was observed. The Gpr3 gene was found to be located on chromosome 6 and a single exon coded for the entire open reading frame. Expression of porcine Gpr3 in HEK293 cells resulted in constitutive activation of adenylate cyclase （AC） similar in amplitude to that produced by fully stimulated Gs coupled receptors. Moreover, sphingosine 1-phosphate （S1P） could increase AC activation via the constitutively active Gpr3 receptor. When a Gpr3-green fluorescent protein （GFP） construct was expressed in HEK293 cells, GFP-labeled Gpr3 protein was shown to be localized in the plasmalemma and subcellular membranes. After S1P treatment, agonist-mediated internalization could be visualized by confocal microscopy. In short, our findings suggest the porcine Gpr3, Gpr6, and Gpr12 genes as a subfamily of G protein-coupled receptors, and porcine Gpr3 was a constitutively active G protein-coupled receptor. Constitutive activation of AG and agonist-mediated internalization of Gpr3 receptor could be modulated by the S1 P, suggesting that S1P might act as an activator for porcine Gpr3 receptor.

关键词： G protein-coupled receptor Constitutive activity Sphingosine 1-phosphate Receptor internalization Porcine Gpr3 Molecular cloning

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An integrated strategy for comprehensive characterization of metabolites and metabolic profiles of bufadienolides from Venenum Bufonis in rats

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Journal of Pharmaceutical Analysis 2022年第1期12卷 136-144页

作者： Wen-Long Wei Hao-Jv Li Wen-Zhi Yang Hua Qu Zhen-Wei Li Chang-Liang Yao Jin-jun hou Wan-Ying Wu De-An Guo Shanghai Research Center for Modernization of Traditional Chinese Medicine National Engineering Laboratory for TCM Standardization TechnologyShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai201203China University of Chinese Academy of Sciences Beijing100049China

Comprehensive characterization of metabolites and metabolic profiles in plasma has considerable significance in determining the efficacy and safety of traditional Chinese medicine(TCM)in ***,this process is usually hindered by the insufficient characteristic fragments of metabolites,ubiquitous matrix interference,and complicated screening and identification procedures for *** this study,an effective strategy was established to systematically characterize the metabolites,deduce the metabolic pathways,and describe the metabolic profiles of bufadienolides isolated from Venenum Bufonis in *** strategy was divided into five ***,the blank and test plasma samples were injected into an ultra-high performance liquid chromatography/linear trap quadrupole-orbitrap-mass spectrometry(MS)system in the full scan mode continuously five times to screen for valid matrix compounds and ***,an extension-mass defect filter model was established to obtain the targeted precursor ions of the list of bufadienolide metabolites,which reduced approximately 39%of the interfering ***,an acquisition model was developed and used to trigger more tandem MS(MS/MS)fragments of precursor ions based on the targeted ion *** acquisition mode enhanced the acquisition capability by approximately four times than that of the regular data-dependent acquisition ***,the acquired data were imported into Compound Discoverer software for identification of metabolites with metabolic network *** main in vivo metabolic pathways of bufadienolides were elucidated.A total of 147 metabolites were characterized,and the main biotransformation reactions of bufadienolides were hydroxylation,dihydroxylation,and ***,the main prototype bufadienolides in plasma at different time points were determined using LC-MS/MS,and the metabolic profiles were clearly *** strategy could be widely used to elucidate the metabolic profiles

关键词： Metabolic profiles Extension-mass defect filter Multidimensional data acquiring Metabolic network prediction Bufadienolides of Venenum Bufonis

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Study on the influence of curcumin on chemosensitivity of nephroblastoma cells

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Asian Pacific Journal of Tropical Medicine 2016年第8期9卷 779-782页

作者： Xiao-Yong Li Yuan-Yuan Feng Wei Dan Deng Pan Guo-Feng Zhang Xian-Liang Wang Guang-jun hou Department of Pediatric Surgery Children's Hospital of Zhengzhou Department of Paediatrics Children's Hospital of Zhengzhou Department of Paediatrics Maternal and Child Health Hospital of Tonglu County

Objective:To study the influence of curcumin on chemosensitivity of nephroblastoma ***:Human nephroblastoma cells line SK-NEP-1 was transplanted to the nude mice subcutaneously to establish the implantation tumor model of human nephroblastoma cells.A total of 30 tumor-bearing mice were divided into three groups of ten *** routine chemotherapy group was given vincristine(0.05 mg/mL·0.2 mL/d) and actinomycin D(15 ng/mL·0.2 mL/d) combined chemotherapy *** curcumin chemotherapy group was given the same combined chemotherapy regimens and curcumin(30 mg/kg/d) by intraperitoneal *** control group was given normal saline(NS) of the same volume by intraperitoneal *** administration would be kept for 4 weeks and 3 days a *** volumetric changes of every group were *** serum of every group in different time was collected and the VEGF content was detected by *** mice were cercrificed and the tumor tissues were stripped and weighed after 4 weeks’ *** tumor inhibition rate was *** cell proliferation activity and apoptosis rate were detected by MTT and flow cytometry *** data were statistically analyzed by SPSS ***:The tumor volume,serum VEGF content,tumor inhibition rate,cell proliferation activity and apoptosis rate of routine chemotherapy group and curcumin chemotherapy group had significant differences comparing with the control group(P<0.05) after 4-week’s *** cancer growth of curcumin chemotherapy group was obviously decreased and even tended to shrink comparing with routine chemotherapy group(χ2=15.732,P=0.007).The cell proliferation activity was significantly reduced and the apoptosis rate was significantly higher,(χ~2=9.427,P=0.012)which showing the effect of chemotherapy was ***:The chemosensitivity of nephroblastoma cells could be improved by curcumin,then the effect of preoperative adjuvant chemotherapy scheme would be enhanced,the gro

关键词： Nephroblastoma Transplanted tumor model Curcumin Chemosensitivity Vincristine

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Hyperlanins A and B, Two Highly Rearranged Meroterpenoids from Hypericum lancasteri

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Chinese Journal of Chemistry 2024年第18期42卷 2180-2186页

作者： Jin-Qiu You Yun-Qi Jin Meng-Yu Li Bo-Chao Zhang Xin-Yu Sun Chun-Yong Ding Jing-Ya Li Ai-jun hou Shanghai Frontiers Science Center of Drug Target Identification and Delivery School of Pharmaceutical SciencesShanghai Jiao Tong UniversityShanghai200240China National Key Laboratory of Innovative Immunotherapy Shanghai Jiao Tong UniversityShanghai 200240China Shanghai Jiao Tong University School of Medicine Shanghai 200025China Shanghai Institute of Materia Medica Chinese Academy of SciencesShanghai 201203China

Hyperlanins A(1)and B(2),two highly rearranged polycyclic polyprenylated acylphloroglucinol(PPAP)-related meroterpenoids based on different new carbon skeletons,were isolated from Hypericum *** 1 incorporates an unprecedented 5/6/7/5 ring system featuring a 3,13-dioxatetracyclo[9.***.1.12.5.01.8]pentadecane *** 2 possesses a unique compact 6/6/5/6/6/5/6 ring system with a caged tetracyclo[6.***.1.13.8.05,11]dodecane *** structures were established by spectroscopic data,X-ray diffraction,and computational *** compounds showed anti-inflammatory activity in *** 1 and 2 could decrease the lipopolysaccharide(LPS)-/nigericin-induced IL-1βrelease in THP-1 *** compounds also showed inhibition in hypoxia-inducible factor-1α(HIF-1αa)pathway luciferase reporter assay.

关键词： Natural products Hypericum lancasteri Meroterpenoids Structureelucidation Inflammation

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生产仿真技术在砂处理系统改造中的应用

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铸造 2001年第2期50卷 52-56页

作者：李彤吴浚郊侯骏清华大学机械工程系北京100084 中国第一汽车集团公司吉林长春130011

本文简要介绍了生产过程仿真的基本概念 ,并结合一条砂处理线改造的实例介绍了生产仿真技术在铸造业中的应用 ,阐述了建立计算机生产仿真模型的过程和基本方法 ,仿真模型的验证 ,以及如何利用仿真模型模拟和评估不同的生产方案。

关键词：生产过程仿真虚拟制造砂处理系统铸造

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Multi-model ensemble learning for battery state-of-health estimation:Recent advances and perspectives

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Journal of Energy Chemistry 2025年第1期100卷 739-759页

作者： Chuanping Lin jun Xu Delong Jiang Jiayang hou Ying Liang Zhongyue Zou Xuesong Mei State Key Laboratory for Manufacturing Systems Engineering Xi'an Jiaotong University Shaanxi Key Laboratory of Intelligent Robots School of Mechanical Engineering Xi'an Jiaotong University Department of Electrical Engineering and Automation Luoyang Institute of Science and Technology School of Mechanical Engineering Hangzhou Dianzi University

The burgeoning market for lithium-ion batteries has stimulated a growing need for more reliable battery performance monitoring. Accurate state-of-health(SOH) estimation is critical for ensuring battery operational performance. Despite numerous data-driven methods reported in existing research for battery SOH estimation, these methods often exhibit inconsistent performance across different application scenarios. To address this issue and overcome the performance limitations of individual data-driven models,integrating multiple models for SOH estimation has received considerable attention. Ensemble learning(EL) typically leverages the strengths of multiple base models to achieve more robust and accurate outputs. However, the lack of a clear review of current research hinders the further development of ensemble methods in SOH estimation. Therefore, this paper comprehensively reviews multi-model ensemble learning methods for battery SOH estimation. First, existing ensemble methods are systematically categorized into 6 classes based on their combination strategies. Different realizations and underlying connections are meticulously analyzed for each category of EL methods, highlighting distinctions, innovations, and typical applications. Subsequently, these ensemble methods are comprehensively compared in terms of base models, combination strategies, and publication trends. Evaluations across 6 dimensions underscore the outstanding performance of stacking-based ensemble methods. Following this, these ensemble methods are further inspected from the perspectives of weighted ensemble and diversity, aiming to inspire potential approaches for enhancing ensemble performance. Moreover, addressing challenges such as base model selection, measuring model robustness and uncertainty, and interpretability of ensemble models in practical applications is emphasized. Finally, future research prospects are outlined, specifically noting that deep learning ensemble is poised to advance ens

关键词： Contrastive Learning

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Alkaloid Constituents of Ficus hispida and Their Antiinfammatory Activity

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Natural Products and Bioprospecting 2020年第1期10卷 45-49页

作者： Xin-Yu Jia Yong-Mei Wu Jing-Ya Li Chun Lei Ai-jun hou School of Pharmacy State Key Laboratory of Medical NeurobiologyFudan UniversityShanghai 201203China National Center for Drug Screening Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

Four new alkaloids,fcuhismines A-D(1-4),together with three known ones,were isolated from Ficus *** structures were elucidated by spectroscopic analysis and chemical *** new compounds represent the frst amine alkaloids with a rhamnosyl moiety(1-2)or with a N-oxide motif(2-4)from the genus *** 2 showed potent inhibitory efect in nuclear factor-κB(NF-κB)pathway luciferase assay with IC_(50) value of 0.52±0.11μM.

关键词： Ficus hispida Moraceae Alkaloids Antiinfammation Nuclear factor-κB

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Red mud and fly ash-based ceramic foams using starch and manganese dioxide as foaming agent

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Transactions of Nonferrous Metals Society of China 2017年第3期27卷 591-598页

作者： Li-jun hou Tao-yong LIU An-xian LU School of Materials Science and Engineering Central South University Changsha 410083 China

Ceramic foams were prepared using red mud and fly ash as raw materials with sodium borate as sintering aid agent,starchand MnO2as foaming agent,*** influence of the amount of starch or MnO2on the crystalline phase,pore morphologyand physical–chemical porosities was *** results showed that the main crystal phases of samples with starch addition andMnO2addition were sodalite phase Na6(AlSiO4)6and Na8(SiAlO4)6MnO4,*** SEM images showed that the variation ofporous structure was mainly dominated by the addition of foaming *** the increase of foaming agent,the samples exhibitedbetter comprehensive properties:bulk density of0.59?0.96g/cm3,porosity of41.82%?63.51%,water absorption of3.16%?9.17%,compressive strength of4.22?8.38MPa,flexural strength of2.44?5.82MPa,acid resistance of95.59%?99.60%,alkali resistance of99.82%?99.99%.Based on these properties,the ceramic foams can be used in building field.

关键词： red mud fly ash starch manganese dioxide ceramic foam

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Polarimetric super-resolution algorithm for radar range imaging via spatial smoothing processing

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Journal of Beijing Institute of Technology 2016年第3期25卷 397-402页

作者： LI Zhang-feng ZHAO Guo-qiang LI Shi-yong LIU Fang SUN hou-jun TAO Ran School of Information and Electronics Beijing Institute of Technology Beijing 100081 China

A full-polarimetric super-resolution algorithm with spatial smoothing processing is presented for one-dimensional（1-D）radar *** coherence between scattering centers is minimized by using spatial smoothing processing（SSP）.Then the range and polarimetric scattering matrix of the scattering centers are *** impact of different lengths of the smoothing window on the imaging quality is mainly analyzed with different signal-to-noise ratios（SNR）.Simulation and experimental results show that an improved radar super-resolution range profile and more precise estimation can be obtained by adjusting the length of the smoothing window under different SNR conditions.

关键词： super-resolution imaging MUSIC imaging polarimetric radar spatial smoothing processing(SSP) signal-to-noise ratio(SNR)

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