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Integration of serum pharmacochemistry with network pharmacology to reveal the potential mechanism of yangqing Chenfei formula(养清尘肺方) for the treatment of silicosis

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Journal of Traditional Chinese Medicine 2024年第4期 784-793页

作者： HU Yuanyuan LIU Xinguang ZHAO Peng WU Jinyan YAN Xinhua hou Runsu WANG Xiangcheng YANG Fan TIAN Xinrong LI Jiansheng Longhua Hospital Shanghai University of Traditional Chinese Medicine Shanghai University of Traditional Chinese Medicine Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan and Education Ministry of P.R.China Henan Key Laboratory of Chinese Medicine for Respiratory Disease Henan University of Chinese Medicine

OBJECTIVE: To explore the mechanisms of yangqing Chenfei formula(养清尘肺方, YCF) in the treatment of silicosis through a comprehensive strategy consisting of serum pharmacochemistry, network pharmacology analysis, and in vitro validation. METHODS: An ultrahigh-performance liquid chromatography-tandem mass spectrometry method was used to confirm the active components in YCF-medicated serum. Then, we obtained targets for active components and genes for silicosis from multiple databases. Furthermore, a protein-protein interaction network was constructed, and Kyoto Encyclopedia of Genes and Genomes pathway and biological process analyses were conducted to elucidate the mechanisms of YCF for the treatment of silicosis. Finally, we validated the important components and mechanisms in vitro. RESULTS: Altogether, 19 active components were identified from rat serum after YCF administration. We identified 724 targets for 19 components, which were mainly related to inflammation [phosphatidy linositol 3 kinase/protein kinase B, forkhead box O, hypoxia inducible factor, and T-cell receptor signaling pathway, nitric oxide biosynthetic process], fibrotic processes [vascular endothelial growth factor signaling pathway, extracellular signal regulated kinase(ERK) 1 and ERK2 cascade, smooth muscle cell proliferation], and apoptosis(negative regulation of apoptotic process). In addition, 218 genes for silicosis were identified and were mainly associated with the inflammatory response and immune process [cytokine-cytokine receptor interaction, tumor necrosis factor alpha(TNF-α), toll-like receptor, and nucleotide binding oligomerization domain-like receptor signaling pathway]. Taking an intersection of active component targets and silicosis genes, we obtained 61 common genes that were mainly related to the inflammatory response and apoptosis, such as the phosphatidylinositol-3-kinase/protein kinase B signaling pathway, mitogen activated protein kinases signaling pathway, TNF signaling pathway, toll-like receptor signaling pathway, biosyn

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Back Frame Section Size Optimization of Large Aperture Telescope

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Journal of Harbin Institute of Technology(New Series) 2023年第4期30卷 76-84页

作者： Qinglong Wu Zhan Yao Tanhui Wu yangqing hou Huazhen Zhang Qian Xu Na Wang Shanghai YueSheng Information Technology Co Ltd.Shanghai 201109China Xinjiang Astronomical Observatory Chinese Academy of SciencesUrumqi 830011China

The back frame structure of a large radio telescope is an important component supporting the reflecting surface,which is directly related to the surface precision.Its optimal design is of key significance for ensuring the surface precision and reducing structural weight.Two methods are constructed to optimize the cross-section size of the telescope back frame in this paper,the criterion method and the first-order optimization method.The criterion method is based on the Lagrangian multiplier method and Kuhn-Tucker condition.This method first establishes the mathematical model by taking the inner and outer radiuses of the back frame beams as the design variables,the structural weight as the constraint condition,and the structural compliance as the objective function,then derives the optimization criterion.The first-order optimization method takes the inner and outer radiuses of the beams as the design variables,the back frame RMS as the objective function,and the structural weight as the constraint condition.Comparison of RMS,structural stress uniformity and optimization efficiency shows that both algorithms can effectively reduce structural deformation and improve RMS,but the criterion method has relatively better result than the first-order method.

关键词： radio telescope back frame size optimization RMS criterion method first-order method

Arrow型蜂窝结构抛物柱面成型蚁狮算法优化

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材料科学与工程学报 2022年第2期40卷 333-339页

作者：姜恒坤张华振候仰青伍科中国空间技术研究院西安分院陕西西安710000 上海跃盛信息技术有限公司上海200240

本研究将Arrow型零泊松比蜂窝结构用于空间抛物柱面天线。首先分析零泊松比蜂窝结构柱面成型所需加载方式,通过几何计算初步得到柱面成型所需载荷;再将ABAQUS分析、曲面拟合和型面精度计算整合为优化目标函数;然后采用蚁狮优化(ALO)算... 详细信息

本研究将Arrow型零泊松比蜂窝结构用于空间抛物柱面天线。首先分析零泊松比蜂窝结构柱面成型所需加载方式,通过几何计算初步得到柱面成型所需载荷;再将ABAQUS分析、曲面拟合和型面精度计算整合为优化目标函数;然后采用蚁狮优化(ALO)算法求解使得型面精度最高的优化载荷,使用有效集法(Active Set)作为对照组,验证蚁狮优化算法的性能。优化结果得到了RMS为0.071 mm的抛物柱面,证明了将零泊松比蜂窝结构用于柱面天线反射面的可行性。与几何计算结果相比,通过ALO算法使得其型面精度提高了45.5%,收敛所需迭代步数为有效集法的1/7,证明了ALO算法对于解决初值未知优化问题的有效性和高效性。研究结果对于零泊松比蜂窝结构的优化设计和ALO算法的应用具有一定意义。

关键词：零泊松比蜂窝结构 Arrow 柱面天线蚁狮优化算法(ALO) 型面精度

GNG4对卵巢癌顺铂耐药A2780/DDP细胞DNA损伤修复及化疗敏感性的影响

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肿瘤研究与临床 2022年第3期34卷 189-193页

作者：夏念李昱曾敏侯金凤葛阳清岳阳市人民医院妇科岳阳414000

目的探讨GNG4与卵巢癌顺铂耐药A2780/DDP细胞DNA损伤修复及化疗敏感性的关系。方法将A2780/DDP细胞分为500 ng/ml顺铂作用组(cDDP组)、短发夹RNA(shRNA)-GNG4沉默GNG4表达组(shRNA组)、500 ng/ml顺铂和shRNA-GNG4干预组(shRNA+cDDP组)、未经顺铂和shRNA-GNG4干预组(空白对照组)。采用蛋白质印迹法检测各组细胞GNG4和γH2AX蛋白的表达,单细胞凝胶电泳法检测各组细胞DNA损伤情况,免疫荧光法检测γH2AX基因在损伤位点的焦点形成情况,平板克隆形成实验检测细胞克隆形成能力。结果与其他3组相比,shRNA+cDDP组GNG4蛋白表达水平最低,γH2AX蛋白表达水平最高,差异均有统计学意义(均P<0.01)。单细胞凝胶电泳法检测显示,空白对照组、cDDP组、shRNA组、shRNA+cDDP组细胞彗星尾DNA百分含量分别为(7.7±2.5)%、(12.3±3.6)%、(20.1±2.1)%、(38.6±2.8)%,Olive尾距分别为5.12±1.89、8.23±2.97、14.99±3.65、22.43±3.17,shRNA+cDDP组细胞彗星尾DNA含量和Olive尾距均高于其他3组,差异均有统计学意义(均P<0.05)。免疫荧光法检测显示,空白对照组、cDDP组、shRNA组、shRNA+cDDP组每个细胞中γH2AX的焦点数分别为(4.2±0.7)个、(5.1±0.5)个、(26.8±3.3)个、(71.3±6.2)个,shRNA+cDDP组均高于其他3组,差异均有统计学意义(均P<0.05)。空白对照组、cDDP组、shRNA组、shRNA+cDDP组克隆形成率分别为(78.27±5.01)%、(45.67±3.29)%、(26.20±5.76)%、(1.56±0.21)%,shRNA+cDDP组均低于其他3组,差异均有统计学意义(均P<0.001)。结论下调GNG4的表达可增加卵巢癌A2780/DDP细胞对顺铂的敏感性,其可能是通过抑制顺铂诱导的DNA损伤修复功能实现的。

关键词：卵巢肿瘤 DNA断裂,双链顺铂 DNA修复 GTP结合蛋白质γ亚单位

yangqing Chenfei formula(养清尘肺方)alleviates crystalline silica induced pulmonary inflammation and fibrosis by suppressing macrophage polarization

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Journal of Traditional Chinese Medicine 2023年第6期43卷 1126-1139页

作者： TIAN Xinrong hou Runsu LIU Xinguang ZHAO Peng TIAN Yange LI Jiansheng Henan Key Laboratory of Chinese Medicine for Respiratory Disease Henan University of Chinese MedicineZhengzhou 450046China Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases co constructed by Henan province and Education Ministry of P.R.China Zhengzhou 450046China Academy of Chinese Medical Sciences Henan University of Chinese MedicineZhengzhou 450046China Department of Respiratory Diseases the First Affiliated Hospital of Henan University of Chinese MedicineZhengzhou 450000China

OBJECTIVE:To explore the underlying mechanisms of the effects of yangqing Chenfei formula(养清尘肺方,YCF) on inflammation and fibrosis in silicosis via inhibition of macrophage polarization.METHODS:A silicotic rat model was established via a single intratracheal instillation of silica particles on the first day of week 0.Subsequently,YCF was administered intragastrically to silicotic rats during weeks 0-2 and 5-8 twice daily.The mouse-derived alveolar macrophage cell line was used to investigate the mechanisms of YCF in M1/M2 polarization.RESULTS:YCF treatment effectively inhibited lung pathological changes,including inflammatory cell infiltration and tissue damage,and increased the forced expiratory volume in the first 0.3 s,functional residual capacity,and maximal mid-expiratory flow in weeks 2 and 8.Furthermore,the treatment improved lung functions by upregulating tidal volume,pause increase,and expiratory flow at 50% tidal volume from weeks 5 to 8.Moreover,YCF could significantly suppressed the progression of inflammation and fibrosis,by reducing the levels of inflammatory cytokines,as well as collagen-Ⅰ and Ⅲ.YCF treatment also decreased the numbers of macrophages and M1/M2 macrophages and the level of transforming growth factor-β(TGF-β).Additionally,YCF5,the effective substance in YCF,decreased lipopolysaccharide and interferon-γ-induced M1 macrophage polarization in a concentration-dependent manner.The mechanism of anti-M1 polarization might be related to a decrease in extracellular signal-regulated kinase,c-JUN N-terminal kinase,P38,and P65 phosphorylation.Furthermore,YCF5 inhibited interleukin-4-induced M2 macrophages by decreasing the protein and m RNA expressions of arginase-1 and CD206 as well as the levels of profibrotic factors,such as TGF-β and connective tissue growth factor.The mechanisms underlying the anti-M2 polarization of YCF5 were primarily associated with the inhibition of the nuclear translocation of phosphorylated signal transducer and activator of transcription 6(pSTAT6).CONCLUSION:YCF signi

关键词： heart failure risk factors fur Yin deficiency cohort studies

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基于数据挖掘的数据库语言查询过程分析

微型电脑应用 2021年第2期37卷 49-52页

作者：侯阳青福州职业技术学院阿里巴巴大数据学院福建福州350108

针对现有数据库语言查询过程分析方法由于切分法不完善,导致查询语句存在着查询准确率低的问题,为此提出基于数据挖掘的数据库语言查询过程分析方法研究。将以查询语句分词数组为基础,通过数据库语义分析识别查询目标与查询条件,构建语... 详细信息

针对现有数据库语言查询过程分析方法由于切分法不完善,导致查询语句存在着查询准确率低的问题,为此提出基于数据挖掘的数据库语言查询过程分析方法研究。将以查询语句分词数组为基础,通过数据库语义分析识别查询目标与查询条件,构建语义依存树,划分为集合块,并通过综合转换算法将自然语言查询转换为SQL语句。在此基础上,利用数据挖掘技术在数据库中定位用户需求数据,提取定位数据并反馈给用户,实现数据库语言查询过程的分析。实验结果显示:针对复杂查询语句来说,所提方法的查询目标与条件识别系数、SQL语句转换系数较大,表明该方法的查询准确率高,能够为用户提供更加便捷、准确的数据库服务。

关键词：数据挖掘数据库语言查询分析

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数据挖掘技术在数控机床可靠性分析中的运用

信息与电脑 2018年第16期30卷 110-112页

作者：侯阳青福州职业技术学院福建福州350108

随着我国经济的快速发展和科技技术的不断提升,信息化技术已在各行业得到广泛应用。其中,数据机床在发展中融合数据挖掘技术,能够有效提升数控机床可靠性。同时,在研究数据机床可靠性时,需对故障模式进行详细分析,并且使用频率方法对故... 详细信息

随着我国经济的快速发展和科技技术的不断提升,信息化技术已在各行业得到广泛应用。其中,数据机床在发展中融合数据挖掘技术,能够有效提升数控机床可靠性。同时,在研究数据机床可靠性时,需对故障模式进行详细分析,并且使用频率方法对故障进行准确分析。此外,数控机床故障信息采集主要由数据库信息系统完成,能够有效提升数控机床可靠性,促使数控机床得到进一步发展。

关键词：数据挖掘技术数控机床可靠性 FMECA方法

反相高效液相色谱法测定关木通及配伍中药中马兜铃酸A

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分析化学 2006年第S1期34卷 161-164页

作者：刘养清赵慧辉侯娜赵平山西中医学院北京中医药大学北京中医医院

为了全面、系统、深入地研究有毒中药关木通,采用乙腈-酸水系统,用反相高效液相色谱法分析关木通及其配伍中药中马兜铃酸A的含量,与文献方法相比,缩短了测定时间,并从总体上降低了分析成本。色谱条件是:C18柱,流速:1mL/min。检测波长:25... 详细信息

为了全面、系统、深入地研究有毒中药关木通,采用乙腈-酸水系统,用反相高效液相色谱法分析关木通及其配伍中药中马兜铃酸A的含量,与文献方法相比,缩短了测定时间,并从总体上降低了分析成本。色谱条件是:C18柱,流速:1mL/min。检测波长:250nm。流动相:乙腈∶(水+冰醋酸)=58∶42。该法精密度高,稳定性好,线性范围为0·070·42μg,回归方程为Y=5·0×106X-32827,相关系数r=0·9999,加样回收率为99·12%。实验结果表明,关木通与生地、当归、大黄、生甘草等中药配伍后,AAⅠ含量均有不同程度的降低,其中以关木通配伍生地组和大黄组最为明显,分别降低了57%和56%。

关键词：关木通马兜铃酸A 反相高效液相色谱

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聚酰亚胺复合膜的泊松比设计

空间电子技术 2022年第2期19卷 88-94页

作者：刘朋博张华振徐斌侯仰青兰澜中国空间技术研究院西安分院西安710000 航天跃盛(杭州)信息技术有限公司上海分公司上海201107

空间薄膜材料的泊松比是张拉中产生膜面褶皱的主要原因之一。为此,需要对薄膜的泊松比进行设计,从而削弱甚至消除薄膜的泊松比效应对膜面精度产生的影响。针对应用于空间结构中的新型正交各向异性纤维增强复合膜,使用正交椭圆孔阵列对... 详细信息

空间薄膜材料的泊松比是张拉中产生膜面褶皱的主要原因之一。为此,需要对薄膜的泊松比进行设计,从而削弱甚至消除薄膜的泊松比效应对膜面精度产生的影响。针对应用于空间结构中的新型正交各向异性纤维增强复合膜,使用正交椭圆孔阵列对复合膜胞元进行泊松比设计,分析其等效的经纬双向泊松比随孔间边界距离和椭圆本身的长短轴之比的变化规律。讨论了打孔后孔间边界距离g,以及椭圆长轴占长短轴之和的比例r,对打孔后薄膜胞元的等效泊松比产生影响的原因。g对等效泊松比的影响主要体现在两个方面:其一是g值影响了胞元的旋转能力,其二是g值决定了材料参数对打孔后的等效泊松比的影响程度。g值越大,等效泊松比越大。r值对等效泊松比的影响主要是胞元的旋转能力,r值越大,旋转能力越大,等效泊松比越小。

关键词：等效泊松比椭圆阵列打孔薄膜胞元旋转经向纬向