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Studies on the bioactivity of marine microorganisms using Pyricularia oryzae model

Acta Oceanologica Sinica 2006年第6期25卷 153-160,161页

作者： WEN Zhanbo PEI Yuehu TIAN Li GAO Aiguo hong xuguang LI Xianchun Qingdao Key Laboratory of Analytical Technology Development and Standardization of Chinese Medicines First Institute of Oceanography State Oceanic Administration Qingdao 266061 China Institute of Microbiology ＆ Epidemiology Academy of Military Medicine Science Beijing 100071 China Shenyang Pharmaceutical University Shenyang 110016 China Qingdao University of Science and Technology Qingdao 266042 China

A total of 2 088 marine microorganisms including 1 392 strains of bacteria and 696 strains of fungi were isolated from diverse marine environment such as the South Pole, the North Pole, and Qingdao tideland, etc. Through a systematic screening process involving the use of Pyricularia oryzae mode, 90 bacteria and 31 fungi strains demonstrating positive bioactivities were identified. The rates of active strains of marine bacteria and marine fungi were 6.5% and 4.5%, respectively. The relationships between the active strains and the sampling locations and hosts were studied. For marine bacteria, the three locations showing the highest rotes of active strains were 8.4% in the Zhujiang Estuary, 7.2% at the North Pole and 6.3% in the Bohai Sea. For marine fungi, the highest rates observed were 7.8% at the South Pole, 7.4% in the South China Sea and 6.8% at the Qingdao tideland. An analysis of the host showed that the three highest rates of active strain for marine bacteria were 8.2% for seawater from the Zhujiang Estuary, 8% for alga from the Qingdao tideland and 7.6% for sea mud from the North Pole. For marine fungi, the highest rates observed were 8.3% for Qingdao actiniae, 7.4% for South China Sea poriferan and 4.5% for soil of Qingdao Suaeda salsa. Four fungi and two bacteria samples were selected for further study because of their high bioactivity. They were found to be active towards several pathogenic microorganisms, and demonstrated stable genetic and thermal characteristics and conservation activities.

关键词： marine microorganisms activitve substances Pyricularia oryzae model

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A preliminary study on benthos food web structure oftidal zone in the Laoshan Bay by using stable carbon isotopes

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Acta Oceanologica Sinica 2000年第4期19卷 81-89页

作者： Cai Deling hong xuguang Mao Xinghua Zhang Shufang Gao Sulan First Institute of Oceanography State Oceanic AdministrationQingdao 266061China

By anayses of carbon isotopic composition (δ13C values) of the benthos collected in the Laoshan Bay in August 1993 and February and May 1994, it is found that the main food sources of the benthos can be divided into four groups in terms of carbon isotope composition: Particulate Organic Matter (POM), benthic diatoms, benthic macroalgae and the organic matter in sediments. The results show that the carbon isotopic composition of the benthic animals has a close relation with that of the food they take in. The Carbon isotopic compositions of benthos may be useful in elucidating their food sources. The carbon isotopic data have confirmed that POM is the main food source of the benthic filter feeders such as bivalves; crustaceans have a wide range of δ13C values, showing their food source has diversity; benthic diatoms are an important fraction of the food for most of gastropods. A preliminary investigation of the benthic-pelagic coupling in that region using the stable carban isotopic tracers has confirmed the importance of POM as a food source for benthos in this region, but the organic matter in sediments and benthic diatoms are also relatively important for a lot of benthic animals. The benthic-pelagic coupling in the Laoshan Bay temperate ecosystem is not so tight as that in ecosystems at higher latitudes such as northeastern water polynya.

关键词： Carbon isotopes food web benthos trophic level

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Characterization of Nutrients,Heavy Metals,Petroleum and Their Impact on Phytoplankton in Laizhou Bay:Implications for Environmental Management and Monitoring

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Journal of Ocean University of China 2024年第3期23卷 822-834页

作者： WANG Kai ZHAO Linlin ZHU Yugui YANG Liqiang WANG Yunfeng hong xuguang College of Marine Life Science Ocean University of ChinaQingdao 266003China First Institute of Oceanography Ministry of Natural ResourcesQingdao 266061China College of Fisheries Ocean University of ChinaQingdao 266003China Institute of Oceanology Chinese Academy of SciencesQingdao 266071China Southern Marine Science and Engineering Guangdong Laboratory Guangzhou 511458China

The Laizhou Bay(LB)represents a substantial ecological area that is vulnerable to human activities and confronts diverse environmental *** study provides a comprehensive characterization of nutrients,petroleum,heavy metals,and phytoplankton community structure across seven distinct areas in *** results indicate relatively high concentrations of NO_(2)-N,SiO_(4)-Si,and NO_(3)-N in the Southwest Laizhou Bay(SWLB)and Huanghe River Estuary(HRE).In contrast,the East Laizhou bay(ELB)and the North of Huanghe River Estuary(NHRE)exhibit the highest concentrations of heavy metals(As,Cr and Hg).The areas with high phytoplankton density and community diversity are mainly located in the *** adjusting for basic environmental factors,phytoplankton density and Margalef richness index D are significantly associated with nutrients(NO_(3)-N,NO_(2)-N,NH_(4)-N,SiO_(4)-Si),and heavy metal(Cr)*** highlight that,in addition to Xiaoqinghe River,nutrients brought by the Mihe River in the SWLB and heavy metal(Cr)pollution in the ELB resulting from industrial and mining activities along the coast significantly influence phytoplankton growth and community ***,it is recommended that more monitoring and management efforts be focused on these regions in the future.

关键词： Huanghe River Estuary Laizhou Bay nutrients heavy metals phytoplankton biodiversity

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Cross-breeding of Different Geological Populations of Scallop, Chlamys farreri

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High Technology Letters 2002年第3期8卷 28-31页

作者：吴谡琦 Sun Xiuqin Zhang Jinxing LI Taiwu hong xuguang First Institute of Oceanography SOA Qingdao 266061 P.R.China Ningbo University Ningbo 315211 P.R.China

Wild populations of Chlamys farreri derived from Japan and China are used as parents in a cross breeding experiment conducted in Zhangzi Island of Liaoning Province in the early May of 2000. Four different first filial generations of the crossbreeding, namely JC, CJ, CC and JJ, which represent different cross combinations of the parents, are differentiated from each other in some observed quantitative traits including survival rate, hatchery rate and attachment rate, etc. By ANOVA analysis, significant differences in growth rate are found among the four populations of the offspring after one year of culturing in Shandong Province. JJ population shows the fastest growth rate and has shell height of 46.1±0.71(SD)mm in average, whereas the other three populations, CC, CJ and JC, has the shell height of 43.1±0.42mm, 41.5±0.48mm and 37.2 ±0.31mm, respectively. No heterosis is detected in the experiment.

关键词： cross breeding scallop Chlamys farreri

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STUDIES ON POLYCHAETA OF THE HAINAN ISLAND WATERS Ⅰ

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海洋科学进展 1993年第4期 57-63页

作者： Meng Fan hong xuguang Wu Baoling (First Institute of Oceanography, SOA, Qingdao,266003)

The polychaete study of the waters of Hainan Island was begun when Wu Baoling collected the specimens of Eunice aphroditois (Pallas) in Lingshui Xincun and Luhuitou and Xizhoudao of Sanya, Hainan Island in 1955. In 1960, a preliminary report of P. V. Uschakov and Wu Baoling on the study of polychaeta in the China seas recorded the representatives of the common polycheates from Hainan, in 1963 V. V. Chlebovitsch published "The Littoral Nereids from Hainan Island" with a description of 29 species. Professor Uschakov described in the Phyllodociformia and Aphroditiformia in Fauna USSR 42 species in all collected from Hainan Island. In 1981 Wu Baoling et al recorded in "The Nereidae of the Chinese Coast" 56 species of nereids from the South China Sea.

关键词：孟凡多毛类 STUDIES ON POLYCHAETA OF THE HAINAN ISLAND WATERS Eu 黄渤海海洋海南岛

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温度刺激下栉孔扇贝不同组织热休克蛋白HSP70的表达研究

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高技术通讯 2005年第5期15卷 96-100页

作者：曲凌云相建海孙修勤洪旭光张进兴中国科学院海洋研究所青岛266071国家海洋局第一海洋研究所青岛266061中国科学院北京研究生院北京100039 中国科学院海洋研究所青岛266071 国家海洋局第一海洋研究所青岛266061

采用免疫印迹(Western blot)和流式细胞术(flow cytometry,FCM)方法,研究了热激栉孔扇贝不同组织内热休克蛋白70(heat shock protein70,HSP70)的表达变化.免疫印迹结果显示,HSP70的诱导表达有组织特异性,即热休克可以使扇贝鳃组织中HSP7... 详细信息

采用免疫印迹(Western blot)和流式细胞术(flow cytometry,FCM)方法,研究了热激栉孔扇贝不同组织内热休克蛋白70(heat shock protein70,HSP70)的表达变化.免疫印迹结果显示,HSP70的诱导表达有组织特异性,即热休克可以使扇贝鳃组织中HSP70的表达明显升高,但在肌柱、外套膜和性腺组织中,未检测到HSP70 的表达.流式细胞术结果表明:在不同的季节,相同的热刺激对扇贝血淋巴中HSP70的诱导表达存在差异.高温刺激后,栉孔扇贝血淋巴细胞中HSP70的表达量逐渐升高,在诱导7h时达到最高.

关键词：栉孔扇贝热休克蛋白 HSP70 免疫印迹流式细胞术组织特异性血淋巴细胞温度刺激

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中国南极长城站室内空气微生物状况

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应用与环境生物学报 2000年第1期6卷 90-92页

作者：陈皓文洪旭光国家海洋局第一海洋研究所青岛266003

The total average value ( n du t(CFU)/ m -3 )of indoor air borne microbial number of Chinese Great Wall Station, Antarctica investigated in Feb. 1994 was 660.2 m -3 , with variation range <26.2～ 7231.2 m -3 . Generally the number of indoor air borne microbes was 233.0 m -3 on the average except under particular circumstances. There were higher mildew detection rates and mildew number/total microbial number in some sampling sites. The variation in the indoor air borne microbial number was related closely with human activities and environmental hygienical conditions. Generally speaking, the indoor air quality of the Great Wall Station was fairly good. But it still need good hygienical habits and more attention to further improve the indoor air conditions and the whole circumstance conditions of the Great Wall Station. Tab 3, Ref

关键词：南极长城站空气微生物环境质量大气卫生

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牙鲆淋巴囊肿病毒核酸疫苗的安全性研究

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高技术通讯 2006年第1期16卷 106-110页

作者：郑风荣孙修勤刘洪展吴谡琦张进兴曲凌云洪旭光中国海洋大学生命科学与技术学部青岛266003 国家海洋局第一海洋研究所生物活性物质实验室青岛266061 山东大学威海分校海洋学院威海264209

对牙鲆淋巴囊肿病毒核酸疫苗进行了安全性鉴定。试验采用PCR方法，通过检测该疫苗是否与牙鲆染色体整合，以及疫苗中的卡那霉素抗性基因是否对牙鲆体内和环境中抗性菌数量及种类产生影响，来确认疫苗的安全性。结果表明，该疫苗不与牙... 详细信息

对牙鲆淋巴囊肿病毒核酸疫苗进行了安全性鉴定。试验采用PCR方法，通过检测该疫苗是否与牙鲆染色体整合，以及疫苗中的卡那霉素抗性基因是否对牙鲆体内和环境中抗性菌数量及种类产生影响，来确认疫苗的安全性。结果表明，该疫苗不与牙鲆染色体发生整合，也未对环境中的抗性苗数量和种类产生影响，表明本实验用核酸疫苗对于受免牙鲆和环境都是安全的。

关键词：核酸疫苗染色体整合抗性基因安全性

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牙鲆淋巴囊肿病毒主要衣壳蛋白基因片段真核表达载体的构建、表达与免疫效果评价

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高技术通讯 2006年第7期16卷 740-745页

作者：孙修勤郑风荣李继业吴兴安曲凌云张进兴洪旭光国家海洋局第一海洋研究所生态室青岛266061 第四军医大学分子病毒实验室西安710032

用淋巴囊肿病毒LCDV-cn感染牙鲆鳃细胞系FG-9307,提取细胞总RNA,用RTPCR法获得了LCDV-cn主要衣壳蛋白（MCP）0.6kb基因片段.将该LCDV-cn-MCP0.6kb片断克隆入真核表达载体pEGFP-N2,得到重组质粒pEGFP-N2-LCDV-cn-MCP 0.6.采用脂质体法将重组质粒转染入牙鲆鳃细胞系FEC,并进行瞬时表达.通过荧光显微镜观察和特异性RT-PCR检测,证实重组质粒pEGFP-N2-LCDV-cn-MCP 0.6kb已成功转染到FEC细胞,并得到了初步表达.将重组质粒肌注入牙鲆体内,检测牙鲆外周血、肠、脾脏、前肾和淋巴细胞的增殖反应、呼吸爆发活性及抗体产生水平.结果表明,构建的核酸疫苗pEGFP-N2-LCDV-cn-MCP 0.6可诱导牙鲆特异性体液免疫和细胞免疫,具有明显的免疫保护作用.

关键词：淋巴囊肿病毒(LCDV) 核酸疫苗真核表达载体构建免疫效果

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军曹鱼淋巴囊肿病毒的系统关系研究及基因型分析

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高技术通讯 2007年第10期17卷 1087-1091页

作者：闫秀英孙修勤吴淑勤吴谡琦张进兴洪旭光郑凤荣曲凌云中国海洋大学生命科学与技术学部青岛266003 国家海洋局第一海洋研究所青岛266061 中国水产科学院珠江水产研究所广州510380

从患淋巴囊肿病的养殖军曹鱼肿瘤中分离到一种虹彩病毒,命名为淋巴囊肿病毒军曹鱼分离株（LCDV-rc）。为探讨LCDV-rc的系统地位,从DNA水平上分析了该病毒株与淋巴囊肿病毒中国牙鲆分离株（LCDV-cn）、淋巴囊肿病毒韩国鲈鱼分离株（LCDV... 详细信息

从患淋巴囊肿病的养殖军曹鱼肿瘤中分离到一种虹彩病毒,命名为淋巴囊肿病毒军曹鱼分离株（LCDV-rc）。为探讨LCDV-rc的系统地位,从DNA水平上分析了该病毒株与淋巴囊肿病毒中国牙鲆分离株（LCDV-cn）、淋巴囊肿病毒韩国鲈鱼分离株（LCDV-sb）、淋巴囊肿病毒日本牙鲆分离株（LCDV-jf）、淋巴囊肿病毒欧洲分离株（LCDV-1）和淋巴囊肿病毒日本岩鱼分离株（LCDV-rf）的系统关系。以核衣壳蛋白（MCP）基因为分子标记,通过PCR扩增和克隆测序获得了长度为1356bp的含178个信息位点的mcp基因序列;用最大简约法、最大似然法、邻接法和Bayesian法构建了分子系统树并进行了置信度检验。结果表明,构建的淋巴囊肿病毒ML、NJ、MP和Bayesian系统树的拓扑结构一致,LCDV-rc和LCDV-sb聚为一支,LCDV-cn和LCDV-jf聚为一支,LCDV-1和LCDV-rf分别各为一支。在35种虹彩病毒的系统关系分析中,LCDV-rc与另5株淋巴囊肿病毒在系统树上聚为一大支,说明LCDV-rc与淋巴囊肿病毒的关系近于其他虹彩病毒。根据遗传距离和系统关系分析认为,LCDV-rc属于一种新的基因型,称其为淋巴囊肿病毒Ⅳ型基因型。

关键词：虹彩病毒淋巴囊肿病毒军曹鱼分离株(LCDV-rc) 系统关系基因型

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