检索结果-南通市图书馆

Time-dependent matrix metalloproteinases and tissue inhibitor of metalloproteinases expression change in fusarium solani keratitis

引用

International Journal of Ophthalmology(English edition) 2016年第4期9卷 512-518页

作者： Qian Li Xin-Rui Gao hong-Ping Cui Li-Li Lang Xiu-Wen Xie Qun Chen Department of Ophthalmology Shanghai East HospitalTongji University School of Medicine Department of Ophthalmology Shanghai Tenth People's Hospital Tenth People's Hospital of Tongji University Department of Ophthalmology Changzhou Third People's Hospital Department of Ophthalmology Shanghai Pudong New Area Gongli Hospital

AIM： To investigate matrix metalloproteinases（MMPs）and tissue inhibitor of metalloproteinases（TIMPs）expression during the progress of fusarium solani（***） keratitis in a rat model.· METHODS： A rat model of *** keratitis was produced using corneal scarification and a hand-made contact lens. MMPs and TIMPs expressiond were explored in this rat model of *** keratitis using real-time polymerase chain reaction（PCR） and ***6001（400 μmol/m L） was used to treat infected corneas. The keratitis duration, amount and area of corneal neovascularization（CNV） were evaluated.·RESULTS： MMP-3 expression was 66.3 times higher in infected corneas compared to normal corneas. MMP-8,-9,and-13 expressions were significantly upregulated in the mid-period of the infection, with infected- to-normal ratios of 4.03, 39.86, and 5.94, respectively. MMP-2.and-7expressions increased in the late period, with the infected-to-normal ratios of 5.94 and 16.2., ***-1 expression was upregulated in the early period,and it was 43.17 times higher in infected compared to normal corneas, but TIMP-2.-3, and-4 expressions were mildly downregulated or unchanged. The results of DIF were consistent with the result of real-time ***6001, a MMPs inhibitor, decreased the duration of *** infection and the amount and area of CNV.·CONCLUSION： MMPs and TIMPs contributed into the progress of *** keratitis.

关键词： fungal keratitis fusarium solani metalloproteinases tissue inhibitors of metalloproteinases

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Survivin和Ki-67在横纹肌肉瘤中的表达及意义

引用

医学研究生学报 2003年第3期16卷 174-176,F003页

作者：郑洪周桥杨光华四川大学华西医院病理研究室四川成都610041

目的 :探讨横纹肌肉瘤 (RMS)中凋亡调控因子Survivin和Ki 6 7的表达及其意义。　方法 :采用免疫组化技术 (S P法 )和图像分析技术检测 4 3例横纹肌肉瘤及 10例正常横纹肌组织中Survivin和Ki 6 7表达水平 ,并结合病理特征进行分析。　结... 详细信息

目的 :探讨横纹肌肉瘤 (RMS)中凋亡调控因子Survivin和Ki 6 7的表达及其意义。　方法 :采用免疫组化技术 (S P法 )和图像分析技术检测 4 3例横纹肌肉瘤及 10例正常横纹肌组织中Survivin和Ki 6 7表达水平 ,并结合病理特征进行分析。　结果 :Survivin在 86 % (37/ 4 3)的RMS中表达 ,且表达水平高于正常横纹肌组织 (P 0 .0 5 ) ,而在不同分化程度的RMS之间差异显著 (P 2.1.4 % )显著高于正常横纹肌组织的Ki 6 7指数 (0 .1% ) ,ERMS和ARMS的Ki 6 7指数差异不显著 (P >0 .0 5 ) ,而在不同分化程度的RMS中 ,Ki 6 7指数差异显著 (P <0 .0 5 )。同样 ,Ki 6 7指数在复发或转移的RMS和无复发或转移的RMS之间的差异显著 (P <0 .0 5 )。　结论 :Survivin表达水平和Ki 6 7指数在RMS中增加 ,其与瘤细胞分化有关。

关键词：横纹肌肉瘤 Survivin Ki-67 免疫组化图像分析技术

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Pan-cancer analysis of DNA epigenetic modifications by hydrophilic interaction liquid chromatography-tandem mass spectrometry

引用

Chinese Chemical Letters 2023年第7期34卷 214-218页

作者： Yiqiu Hu Xiujuan hong Zhijun Yuan Jiayi Mu Xiaoxiao Zhang Zhihao Fang Ying Yuan Shu Zheng Cheng Guo Cancer Institute(Key Laboratory of Cancer Prevention and Intervention China National Ministry of Education)The Second Affiliated HospitalZhejiang UniversitySchool of MedicineHangzhou310009China Cancer Center Zhejiang UniversityHangzhou 310058China

Accumulating evidence in recent years indicates that DNA methylation(5-methyl-2.deoxycytidine,5-mdC) and hydroxymethylation(5-hydroxymethyl-2.deoxycytidine, 5-hmd C) have been implicated in various biological processes, and the aberrations of these DNA cytosine modifications is tightly associated with cancer. N6-methyl-2.deoxyadenosine(m~6dA), as a newly discovered epigenetic modification in genome of mammals, has been demonstrated to play vital regulatory roles in tumorigenesis. However, the content information of m~6dA in human tumor tissues is still limited and pan-cancer analysis of these DNA epigenetic modifications is lacked. Herein, we developed a sensitive and robust stable isotopediluted hydrophilic interaction liquid chromatography-tandem mass spectrometry(HILIC-MS/MS) method for accurate quantification of m~6dA, 5-mdC and 5-hmdC in genomic DNA from 82.pairs of human tumor tissues and matched tumor-adjacent normal tissues. The types of tumors included esophagus cancer, lung cancer, breast cancer, liver cancer, pancreatic cancer, gastric cancer, stromal tumor and colorectal *** to the normal tissues, we revealed the level of m6dA was increased in tumor tissues of esophagus cancer, lung cancer and liver cancer, whereas the level of m~6dA was diminished in tumor tissues of pancreatic cancer and gastric cancer;while the contents of 5-mdC and 5-hmdC exhibited significant decrease in tumor tissues of most types of cancer. It is worth noting that we revealed, for the first time,the content of genomic m~6dA in pancreatic cancer, stromal tumor and colorectal cancer. The significant changes of these DNA epigenetic modifications indicate they may serve as indicators of cancers. In addition, this study will benefit for better understanding of the regulatory roles of these DNA epigenetic modifications in cancers.

关键词： HILIC-MS/MS Pan-cancer analysis N~6-methyl-2.deoxyadenosine 5-Methyl-2.deoxycytidine 5-Hydroxymethyl-2.deoxycytidine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Tumor-homing peptide-based NIR-II probes for targeted spontaneous breast tumor imaging

引用

中国化学快报：英文版 2020年第6期31卷 1382-1386,F0002页

作者： Hui Zhou Shanshan Li Xiaodong Zeng Mengxian Zhang Lin Tang Qianqian Li Deliang Chen Xianli Meng Xuechuan hong Innovative Institute of Chinese Medicine and Pharmacy Chengdu University of Traditional Chinese Medicine.Chengdu 611137China Key Laboratory of Combinatorial Biosynthesis and Drug Discovery(MOE) Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals.Shenzhen Institute of Wuhan UniversityShenzhen 518057China College of Science Innovation Center for Traditional Tibetan Medicine Modernization and Quality ControlMedical CollegeTibet UniversityLasa 850000China Jiangxi Key Laboratory of Organo-Pharmaceutical Chemistry Chemistry and Chemical Engineering CollegeGannan Normal UniversityGanzhou 341000China

Fluorescence imaging in the second near-infrared window(NIR-Ⅱ,1000-1700 nm)is a promising modality for real-time imaging of cancer and image-guided surgery with superior in vivo optical *** far,very few NIR-Ⅱfluorophores have been reported for in vivo biomedical imaging of chemically-induced spontaneous breast ***,a NIR-Ⅱfluorescent probe CH1055-F3 with the nucleolin-targeted tumor-homing peptide F3 was demonstrated to prefe rentially accumulate in 4 T1 *** importantly,CH1055-F3 exhibited specific NIR-Ⅱsignals with high spatial and temporal resolution,strong tumor uptake,and remarkable NIR-Ⅱimage-guided surgery in dimethylbenzanthracene(DMBA)-induced spontaneous breast tumor *** report presents the first tumor-homing peptide-based NIR-Ⅱprobe to diagnose transplantable and spontaneous breast tumors by the active targeting.

关键词： NIR-Ⅱimaging F3 peptide Image-guided surgery Spontaneous breast carcinoma NIR-Ⅱprobes

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

树突状细胞在榄香烯复合瘤苗主动免疫效应中的作用

引用

上海免疫学杂志 2003年第4期23卷 225-227,231页

作者：屈虹郭连英施广霞李丹朴花沈洁钱振超大连医科大学肿瘤生物治疗研究所大连116027

本实验以HCa F或L6 1 5榄香烯复合瘤苗 (H TCV或L TCV )、H TCV溶解物 (TH )、短小棒状菌 (CP )免疫小鼠 ,分离制备其脾脏DC ,在体外分别用相应瘤细胞溶解物 (H或L )和瘤苗溶解物 (TH或TL )冲激后 ,以MTT法检测其诱导同系小鼠脾不粘附... 详细信息

本实验以HCa F或L6 1 5榄香烯复合瘤苗 (H TCV或L TCV )、H TCV溶解物 (TH )、短小棒状菌 (CP )免疫小鼠 ,分离制备其脾脏DC ,在体外分别用相应瘤细胞溶解物 (H或L )和瘤苗溶解物 (TH或TL )冲激后 ,以MTT法检测其诱导同系小鼠脾不粘附细胞增殖的能力。结果表明用榄香烯复合瘤苗或其溶解物免疫DC供鼠和体外冲激DC ,可增强其诱导同系小鼠脾不粘附细胞增殖的作用。

关键词：树突状细胞榄香烯复合瘤苗抗原递呈

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

An improved method for the investigation of high-order harmonic generation from graphene

引用

Chinese Physics B 2020年第10期29卷 268-275页

作者： Zhong Guan Lu Liu Guo-Li Wang Song-Feng Zhao Zhi-hong Jiao Xiao-Xin Zhou College of Physics and Electronic Engineering Northwest Normal UniversityLanzhou 730070China Department of Physics College of ScienceNational University of Defense TechnologyChangsha 410073China

High-order harmonic generation (HHG) of bulk crystals in strong laser field is typically investigated with semiconductor Bloch equations (SBEs). However, in the length gauge, it suffers from the divergence for the crystals with a zero band gap, such as graphene, using both Bloch- and Houston-states expansion methods. Here, we present a method of solving the SBEs based on time-dependent Bloch basis, which is equivalent to semiconductor Bloch equations in the velocity gauge. Using this method, we investigate the HHG of a single-layer graphene. It is found that our results for population are in good agreement with the other results. For a initial condition py = 0, we find the electrons just move in single valence band or conduction band, which are in accord with classical results. Our simulations on the HHG dependence of polarization of driving laser pulse confirm that 5th, 7th, and 9th harmonic yields increase to the maximal value when laser ellipticity ε ≈ 0.3. What is more, similar to the case of atoms in the laser field, the total strength of 3rd harmonic decrease monotonically with the increase of ε. In addition, we simulate the dependence of HHG on crystallographic orientation with respect to the polarization direction of linear mid-infrared laser pulse, and the results reveal that for higher harmonics, their radiation along with the change of rotation angle θ reflects exactly the sixfold symmetry of graphene. Our method can be further used to investigate the behaviors of other materials having Dirac points (i.e., surface states of topological insulators) in the strong laser fields.

关键词： high-order harmonic generation graphene velocity gauge divergence

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Light-fueled dissipative self-assembly at molecular and macro-scale enabled by a visible-light-responsive transient hetero-complementary quadruple hydrogen bond

引用

Chinese Chemical Letters 2023年第3期34卷 308-312页

作者： Shi-Tao Han hong-Ying Duan Tian-Guang Zhan Xiao-Bo Hu Li-Chun Kong Kang-Da Zhang Key Laboratory of the Ministry of Education for Advanced Catalysis Materials College of Chemistry and Life ScienceZhejiang Normal UniversityJinhua 321004China Key Laboratory of Precise Synthesis of Functional Molecules of Zhejiang Province School of ScienceWestlake UniversityHangzhou 310024China

The development of out-of-equilibrium self-assembly systems using light as input fuel is highly desirable and promising for the fabrication of smart supramolecular materials. Herein, we report the construction of new artificial light-fueled dissipative molecular and macroscopic self-assembly systems based on a visible-light-responsive transient quadruple H-bonding array, which consists of an azobenzene-modified ureidopyrimidinone(UPy) module(Azo-O-UPy) and a nonphotoactive diamidonaphthyridine(DAN) derived competitive binder(Napy-1). The visible light(410 nm) irradiation can induce the E to Z isomerization of the azobenzene unit of E-Azo-O-UPy to produce Z-Azo-O-UPy with an opened UPy binding site, which can complex with Napy-1 to form a quadruply H-bonded heterodimer. The heterodimer is metastable and can be quickly disassembled in dark, owing to the fast thermal relaxation of Z-Azo-O-UPy to E-Azo-O-UPy. While introducing such transient quadruple H-bonding interaction into a linear polymer system or a polymeric gel system, light-fueled out-of-equilibrium polymeric assembly both at molecular and macro-scale can be achieved.

关键词： Dissipative self-assembly Macroscopic self-assembly Photo-responsiveness Quadruple hydrogen bonds Supramolecular chemistry

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Probing top-philic new physics via four-top-quark production

引用

Chinese Physics C 2021年第9期45卷 81-87页

作者： Qing-hong Cao Jun-Ning Fu Yandong Liu Xiao-Hu Wang Rui Zhang Department of Physics and State Key Laboratory of Nuclear Physics and Technology Peking UniversityBeijing 100871China Collaborative Innovation Center of Quantum Matter Beijing 100871China Center for High Energy Physics Peking UniversityBeijing 100871China Key Laboratory of Beam Technology of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China beijing Radiation Center Beijing 100875China Theoretical Physics Division Institute of High Energy PhysicsBeijing 100049China

We explore constraints on various new physics resonances from four top-quark production based on current experimental *** light and heavy resonances are studied in this work.A comparison of the full width effect and n... 详细信息

关键词： top quark new physics collider physics

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

社区护理干预对高体重指数人群的影响

引用

实用全科医学 2008年第1期6卷 70-71页

作者：孙洪梅朱翠凤孟繁华邵梅陈苹苹北京大学深圳医院大学城社康中心广东省深圳市518000

目的探讨社区护理干预对高体重指数人群的影响。方法随机抽取40名体重指数(BMI)≥2.的人群为其实施护理干预,量身定做运动处方,同时给予膳食指导,观察前后体重指数变化。结果实施对策5个月后,监测各项指标,体重指数明显降低,差异具有统... 详细信息

目的探讨社区护理干预对高体重指数人群的影响。方法随机抽取40名体重指数(BMI)≥2.的人群为其实施护理干预,量身定做运动处方,同时给予膳食指导,观察前后体重指数变化。结果实施对策5个月后,监测各项指标,体重指数明显降低,差异具有统计学意义(P<0.01)。结论对高体重指数人群实施护理干预既经济又安全,可帮助社区人树立新的健康观念,自愿采纳有利于健康的行为和生活方式,增进社区人的健康,防治疾病发生。

关键词：社区护理干预体重指数运动处方

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Room temperature non-balanced electric bridge ethanol gas sensor based on a single ZnO microwire

引用

Chinese Physics B 2020年第1期29卷 463-468页

作者： Yun-Zheng Li Qiu-Ju Feng Bo Shi Chong Gao De-Yu Wang hong-Wei Liang School of Physics and Electronic Technology Liaoning Normal UniversityDalian 116029China School of Microelectronics Dalian University of TechnologyDalian 116024China

In this paper,ultra-long and large-scaled ZnO microwire arrays are grown by the chemical vapor deposition method,and a single ZnO microwire-based non-balanced electric bridge ethanol gas sensor is *** experimental results show that the gas sensor has good repeatability,high response rate,short response,and recovery time at room temperature(2.℃).The response rate of the gas sensor exposed to 90-ppm ethanol is about 93%,with a response time and recovery time are 0.3 s and 0.7 s *** a contrast,the traditional resistive gas sensor of a single ZnO microwire shows very small gas response ***,ethanol gas sensor based on non-balanced electric bridge can obviously enhance gas sensing characteristics,which provides a feasible method of developing the high performance ZnO-based gas sensor.

关键词： ZnO microwire gas sensor room temperature ethanol

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：