检索结果-南通市图书馆

检索条件"作者=HILLESHEIM j"

共 1 条记录，以下是1-10 订阅

全选清除本页清除全部题录导出标记到"检索档案"

详细简洁

排序：

Defective jejunal and colonic salt absorption and altered Na+/H+ exchanger 3 (NHE3) activity in NHE3 regulatory factor 1(NHERF1) adaptor protein deficient mice

Defective jejunal and colonic salt absorption and altered Na...

引用

2008北京国际生理科学学术大会

作者： BROERE N CINAR A SINGH A.K hillesheim j BIEDERER B LNNEMANN M ROTTINGHAUS I KRABBENHfT A ENGELHARDT R RAUSCH B WEINMAN E.j DONOWITZ M HUBBARD A KOCHER O DE jONGE H.R HOGEMA B.M SEIDLER U Department of Biochemistry Erasmus University Medical Center3015GE Rotterdamthe Netherlands Department of Gastroenterology Hannover Medical SchoolHannover D-30625Germany Departments of Medicine and Physiology University of Maryland School of MedicineMaryland 21201 Departments of Medicine PhysiologyJohns Hopkins University School of MedicineBaltimoreMaryland21205-2195 Departement of Cell Biology Johns Hopkins University School of MedicineBaltimoreMaryland21205-2195 Department of Pathology Beth Israel Deaconess Medical Center and Harvard Medical SchoolBostonMA 02215

We investigated the role of the Na/H exchanger 3(NHE3)regulatory factor 1(NHERFl)on intestinal salt and water absorption,brush border membrane morphology and on the NHE3 mRNA expression,protein abundance,and transport activity in the murine ***1-deficient mice displayed reduced jejunal fluid absorption in vivo,as well as a attenuated Na absorption in isolated mucosa of jejunal and colonic tissue but not of ileal mucosa in ***,cAMP-mediated inhibition of both parameters remained *** basal NHE3 transport rate was decreased in surface colonocytes,while inhibition by cAMP and cGMP was normal. Immunodetection of NHE3 revealed normal NHE3 localization in the brush border membrane(BBM)of NHERF1 null mice,but NHE3 abundance,as measured by Western blot,was significantly reduced in isolated membranes from the small and large ***,the microvilli in the proximal colon,but not in the small intestine, were significantly shorter in NHERF1 null *** knockout of PDZK1(NHERF3),another member of the NHERF family of adapter proteins,which binds to both NHE3 and NHERF1,further reduced basal NHE3 activity and caused complete loss of cAMP-mediated NHE3 *** activator of the exchange protein activated by cAMP(EPAC)had no effect on jejunal fluid absorption in vivo,but slightly inhibited NHE3 activity in surface colonocytes in *** conclusion,NHERF1 has segment-specific effects on intestinal salt absorption,NHE3 transport rates and NHE3 membrane abundance without affecting mRNA ***,unlike PDZK1,NHERF1 is not required for NHE3 regulation by cyclic nucleotides.

关键词： PDZ domain proteins intestinal electrolyte transport sodium absorption colon small intestine PDZK1 NHERF1 knockout mice

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

全选清除本页清除全部题录导出标记到“检索档案”

共1页 << < 1 > >>

回到顶部

执行限定条件

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：