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Expression of a soluble form of CTLA4 on macrophage and its biological activity

Cell Research 1999年第3期9卷 189-199页

作者： GAO YUE HUA PICHAO WANG KUNIAKI TAKAGI OSAMU SHlMOZATO hideo yagita TOHRU OKIGAKI MASAfOSHI MATASUMURA(Institute of APPlied Biochemistry, University ofTsukuba, Ibamki 305-8572, Japan)(Department of Environmental Biochemistry, Schoolof Pharmaceutical Scie Institute of Applied Biochemistry University of Tsukuba Ibaraki Japan Department of Environmental Biochemistry School of Pharmaceutical Science Univer sity of Shizuoka Shizuoka Japan Department of Immunology Juntendo University School of Medicine Tokyo Japan Division of Molecular and Cell Biology Shigei Medical Research Institute Okayama Japan

Interaction between cytotoxic T lymphocyte-asso-ciated antigen-4 (CTLA4, CD152) and B7 molecules (B7-1and B7-2) is of importance in the cellular events of lym-phocyte, including antigen-specific T-cell activation andinduction of autoreactive T-cell. We describe here the firstintroduction of a murine soluble CTLA4 gene, CTLA4Ig,to Mm1 cells, a macrophagic cell line. CTLA4Ig waJssuccessfully expressed on Mm1 cells and the expressedCTLA4Ig was found to be functionally active in their bind-ing to B7 molecules by flow cytometry and immunofluo-rescence studies- The biological activity of CTLA4Ig fromthe transfected Mm1 cells was studied and showed in-hibitory activity on mixed lymphocyte culture. A highCTLA4Ig producing macrophagic cell line was *** Mm1 cells were regarded as difficult for gene transfec-tion and there had so far been no report on expression ofCTLA4Ig gene on Mm1 cells, these results suggested thatthe CTLA4Ig expressing Mm1 cells could be useful forExpression of CTLA4 on Mml and its biological activityanalysis of CTLA4 and B7 molecule interaction in bothmacrophage and T-cell.

关键词： Macrophage fusion protein CTLA4 B7 transfection

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