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Dual-objective two-photon microscope for volumetric imaging of dense scattering biological samples by bidirectional excitation and collection

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Photonics Research 2024年第6期12卷 1351-1361页

作者： MUYUE ZHAI JING YU YANHUI HU HANG YU BEICHEN XIE YI YU DAWEI LI AIMIN WANG heping cheng National Biomedical Imaging Center College of Future TechnologyPeking UniversityBeijing 100871China Research Unit of Mitochondria in Brain Diseases Chinese Academy of Medical SciencesPKU-Nanjing Institute of Translational MedicineNanjing 211500China Beijing Transcend Vivoscope Biotech Beijing 100085China College of Engineering Peking UniversityBeijing 100871China Academy for Advanced Interdisciplinary Studies Peking UniversityBeijing 100871China Center for Quantitative Biology Peking UniversityBeijing 100871China School of Electronics Peking UniversityBeijing 100871China State Key Laboratory of Advanced Optical Communication System and Networks Peking UniversityBeijing 100871China

Full view observation throughout entire specimens over a prolonged period is crucial when exploring the physiological functions and system-level ***-photon microscopy(MPM)has been widely employed for such purposes owing to its deep penetration ***,the current MPM struggles with balancing the imaging depth and quality while avoiding photodamage for the exponential increasement of excitation power with the imaging ***,we present a dual-objective two-photon microscope(Duo-2P),characterized by bidirectional two-photon excitation and fluorescence collection,for long-duration volumetric imaging of dense scattering ***-2P effectively doubles the imaging depth,reduces the total excitation energy by an order of magnitude for samples with a thickness five times the scattering length,and enhances the signal-to-noise ratio up to 1.4 *** these advantages,we acquired volumetric images of a 380-μm suprachiasmatic nucleus slice for continuous 4-h recording at a rate of 1.67 s/volume,visualized the calcium activities over 4000 neurons,and uncovered their state-switching *** conclude that Duo-2P provides an elegant and powerful means to overcome the fundamental depth limit while mitigating photodamages for deep tissue volumetric imaging.

关键词： excitation overcome exponential

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铁尾矿沥青混凝土性能研究和红外光谱分析

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矿产综合利用 2024年第3期45卷 143-149页

作者：王丽王有涛程和平内江职业技术学院土木工程系四川内江641000 贵州省遵义公路管理局贵州遵义563000 辽宁工程技术大学土木工程学院辽宁阜新123000 常州工程职业技术学院建筑工程学院江苏常州213164

这是一篇陶瓷及复合材料领域的论文。本文分析了不同铁尾矿掺量的沥青混合料的性能,并对沥青混合料的抵抗车辙性能、水稳定性、抗裂性能进行分析,进而验证铁尾矿应用于低等级道路中面层的可行性。结果表明:弯拉强度在在掺量从20%到40%... 详细信息

这是一篇陶瓷及复合材料领域的论文。本文分析了不同铁尾矿掺量的沥青混合料的性能,并对沥青混合料的抵抗车辙性能、水稳定性、抗裂性能进行分析,进而验证铁尾矿应用于低等级道路中面层的可行性。结果表明:弯拉强度在在掺量从20%到40%时下降幅度较大;浸水残留稳定度在掺量为20%后增长幅度开始下降。随着铁尾矿掺量不断增大,铁尾矿沥青混凝土FT-IR图谱在局部出现了较小的、新的吸收峰且对原有吸收峰也出现了增强,这说明了掺入铁尾矿后,沥青混凝土与其发生了化学反应,且改变了原有沥青内部结构,进而会产生新的官能团。随着铁尾矿掺量不断增大,铁尾矿沥青混凝土沥青环烷烃和烷烃C-H官能团面积占比却呈现出先增大后减小的趋势,且铁尾矿掺量为20%时,沥青环烷烃和烷烃C-H官能团面积占比达到较大。

关键词：陶瓷及复合材料铁尾矿抗车辙性能水稳定性抗裂性能吸收峰

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Visualizing Seizure Propagation in Freely-moving Mice via Miniature Two-photon Microscopy

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Neuroscience Bulletin 2022年第12期38卷 1593-1597页

作者： Zhuoran Zhang Shihe Jiang Kaibin Shi Yan Li Wei‑Na Jin Qiang Liu Ting Zhao heping cheng Fu‑Dong Shi Department of Neurology Tianjin Medical University General HospitalTianjin 300052China Center for Neurological Diseases China National Clinical Research Center for Neurological DiseasesBeijing Tiantan HospitalCapital Medical UniversityBeijing 100070China Nanjing Brain Observatory PKU-Nanjing Institute of Translational MedicineNanjing 211899China State Key Laboratory of Membrane Biology Institute of Molecular MedicinePeking-Tsinghua Center for Life SciencesCollege of Future TechnologyPeking UniversityBeijing 100091China

DearEditor,Due to the tight and precise associations between behaviors and neural activities,the investigation of neuronal activity in freely-movingand behavinganimals isamajor unaccomplished goal in *** seizures,characterized by hyper-synchronized discharges and hyper-excitation in neuronal networks,are one of the most common neurological disorders.

关键词： moving precise excitation

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A novel stochastic reaction-diffusion model of Ca^2＋ blink in cardiac myocytes

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Science Bulletin 2017年第1期62卷 5-8页

作者： Jinghui Li Wenjun Xie Xi Chen Yunlong Huo heping cheng Wenchang Tan state key laboratory of turbulence and complex systems and department of mechanics and engineering science college of engineeringpeking universityBeijing 100871China institute of molecular medicine and national laboratory of biomembrane and membrane biotechnology peking universityBeijing 100871China shenzhen graduate school peking universityShenzhen 518055China

In cardiac myocytes,the sarcoplasmic reticulum(SR)is the main storage organelle of free Ca^(2+).The concentration of free Ca^(2+)in the SR is 0.5–1.0 mmol/L and is 2–3 orders of magnitude greater than that in the *** SR is composed of interconnected cisternae(junctional SR,i.e.,JSR)and tubules(free SR network,i.e.,FSR)that extend throughout the cytosol[1].Ca^(2+)is released from the JSR into the cytosol via Ca^(2+)release units(CRUs,

关键词：心肌细胞反应扩散模型随机 Ca2+ 钙贮存器细胞内肌浆网

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Regulation of superoxide flashes by transient and steady mitochondrial calcium elevations

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Science China(Life Sciences) 2014年第5期57卷 495-501页

作者： JIAN ChongShu HOU TingTing YIN RongKang cheng heping WANG XianHua State Key Laboratory of Biomembrane and Membrane Biotechnology Beijing Key Laboratory of Cardiometabolic Molecular MedicineInstitute of Molecular MedicinePeking-Tsinghua Center for Life SciencesPeking University

The mitochondria play essential roles in both intracellular calcium and reactive oxygen species *** a newly discovered universal and fundamental mitochondrial phenomenon,superoxide flashes reflect transient bursts of superoxide production in the matrix of single *** and how the superoxide flash activity is regulated by mitochondrial calcium remain largely *** we demonstrate that elevating mitochondrial calcium either by the calcium ionophore ionomycin or by increasing the bathing calcium in permeabilized HeLa cells increases superoxide flash incidence,and inhibition of the mitochondrial calcium uniporter activity abolishes the flash ***,the superoxide flash incidence is correlated to the steady-state mitochondrial calcium elevation with 1.7-fold increase per 1.0?F/F0 of Rhod-2 *** contrast,large mitochondrial calcium transients(e.g.,peak△F/F0~2.8,duration^2 min)in the absence of steady-state elevations failed to alter the flash *** results indicate that physiological levels of sustained,but not transient,mitochondrial calcium elevation acts as a potent regulator of superoxide flashes,but its mechanism of action likely involves a multi-step,slow-onset process.

关键词：线粒体钙调节稳态超氧闪烁瞬态细胞内钙离子 HeLa细胞

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Miniature Fluorescence Microscopy for Imaging Brain Activity in Freely-Behaving Animals

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Neuroscience Bulletin 2020年第10期36卷 1182-1190页

作者： Shiyuan Chen Ziehen Wang Dong Zhang Aiming Wang Liangyi Chen heping cheng Runlong Wu State Key Laboratory of Membrane Biology Institute of Molecular MedicinePeking-Tsinghua Center for Life SciencesPKU-Nanjing Institute of Translational MedicinePeking UniversityBeijing 100871China Academy of Advanced Interdisciplinary Study Peking UniversityBeijing 100871China Department of Electronics Peking UniversityBeijing 100871China State Key Laboratory of Advanced Optical Communication System and Networks Peking UniversityBeijing 100871China

An ultimate goal of neuroscience is to decipher the principles underlying neuronal information processing at the molecular,cellular,circuit,and system *** advent of miniature fluorescence microscopy has furthered the quest by visualizing brain activities and structural dynamics in animals engaged in self-determined *** this brief review,we summarize recent advances in miniature fluorescence microscopy for neuroscience,focusing mostly on two mainstream solutions-miniature single-photon microscopy,and miniature two-photon *** discuss their technical advantages and limitations as well as unmet challenges for future *** of preliminary applications are also presented to reflect on a new trend of brain imaging in experimental paradigms involving body movements,long and complex protocols,and even disease progression and aging.

关键词： Miniature fluorescence microscopy Brain imaging Two-photon microscopy Neuronal information processing

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铁尾矿沥青混凝土混合料的路用特性研究

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金属矿山 2022年第3期51卷 232-236页

作者：程和平陆璐常州工程职业技术学院建筑工程学院江苏常州213164 辽宁工程技术大学土木工程学院辽宁阜新123000

为探究不同铁尾矿掺量下沥青混凝土的路用特性,以齐大山铁尾矿部分替代天然砂为细骨料,考察了铁尾矿掺量对沥青混凝土混合料软化点、高温稳定性、冻融劈裂、抗车辙性以及低温抗裂性的影响。结果表明:①随着铁尾矿掺量的增大,试样软化点... 详细信息

为探究不同铁尾矿掺量下沥青混凝土的路用特性,以齐大山铁尾矿部分替代天然砂为细骨料,考察了铁尾矿掺量对沥青混凝土混合料软化点、高温稳定性、冻融劈裂、抗车辙性以及低温抗裂性的影响。结果表明:①随着铁尾矿掺量的增大,试样软化点先增大后趋于稳定,动稳定度先升高后降低,铁尾矿掺量为20%时沥青混凝土混合料的软化点及动稳定度最高,分别达56℃、5292次/mm。②随着铁尾矿掺量的增大,试样最大弯曲应变先减小后增大,抗弯强度逐渐增大;最大弯曲应变值在铁尾矿掺量为30%时达到了最低,且此时试样的性能基本能够满足路面性能的要求。③随着铁尾矿掺量的增大,试样抗车辙因子先增大后略微降低,铁尾矿掺量为20%时达到最大值。④随着冻融循环次数的增加,试样劈裂强度比逐渐降低;在同一冻融循环次数作用下,随着铁尾矿掺量的增大,沥青试样的劈裂强度比呈现出先增大后减小的趋势。综合铁尾矿沥青混凝土试验结果可知,当铁尾矿掺量为20%~30%时,沥青混凝土的各项指标可达到最佳。

关键词：铁尾矿掺量高温稳定性低温抗裂性冻融劈裂抗车辙性劈裂强度比

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Triple reverse order law for the Drazin inverse

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Applied Mathematics(A Journal of Chinese Universities) 2024年第1期39卷 55-68页

作者： WANG Hua ZHONG cheng-cheng College of Sciences Inner Mongolia University of TechnologyHohhot 010051China College of Engineering University of Jinan Quancheng CollegeYantai 265600China

In this paper,we investigate the reverse order law for Drazin inverse of three bound-ed linear operators under some commutation ***,the Drazin invertibility of sum is also obtained for two bounded linear operators and... 详细信息

关键词： Drazin inverse reverse order law space decomposition

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Photocatalytic activation of sulfite by N-doped porous biochar/MnFe_(2)O_(4) interface-driven catalyst for efficient degradation of tetracycline

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Green Energy & Environment 2024年第3期9卷 481-494页

作者： Long cheng Yuanhui Ji Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research School of Chemistry and Chemical EngineeringSoutheast UniversityNanjing211189China

A novel photo-catalytic system composed of N-doped biochars(NBCs),MnFe_(2)O_(4) and sulfite activation under ultraviolet(NBCs/MnFe_(2)O_(4)/sulfite/UV)was constructed to realize the efficient eliminate of tetracycline(TC).As the carrier of MnFe_(2)O_(4),NBCs were synthesized from alfalfa,which has large specific surface area,graphite like structure and hierarchical porous *** adsorption isotherm indicated that NBCs/MnFe_(2)O_(4)-2:1 had the best adsorption performance for TC(347.56 mg g^(-1)).Through synergistic adsorption and photocatalysis,the removal rate of TC reached 84%,which was significantly higher than that of MnFe_(2)O_(4).Electrochemical impedance spectroscopy(EIS)and Photoluminescence(PL)characterization results showed that the introduction of NBCs improved the separation efficiency of photogenerated electron and hole pairs and enhanced the photocatalytic ***,the adsorption,degradation mechanism and degradation path of TC by the catalyst were systematically analyzed by coupling HPLC–MS measurement with the theoretical *** the advantages of excellent degradation performance,low cost,easy separation and environmental friendliness of NBCs/MnFe_(2)O_(4),this work was expected to provide a new path for the practical application of biochar.

关键词： Biochar MnFe_(2)O_(4) Sulfite Adsorption Photocatalysis

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An improved initial rotor position estimation method using high-frequency pulsating voltage injection for PMSM

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Defence Technology（防务技术） 2024年第3期33卷 19-29页

作者： Yang Jiang Ming cheng School of Electrical Engineering Southeast UniversityNanjing 210096China

High frequency pulsating voltage injection method is a good candidate for detecting the initial rotor position of permanent magnet synchronous ***,traditional methods require a large number of filters,which leads to the deterioration of system stability and dynamic *** order to solve these problems,a new signal demodulation method is proposed in this *** proposed new method can directly obtain the amplitude of high-frequency current,thus eliminating the use of filters,improving system stability and dynamic performance and saving the work of adjusting filter *** addition,a new magnetic polarity detection method is proposed,which is robust to current measurement ***,experiments verify the effectiveness of the method.

关键词： Initial position detection Signal demodulation algorithm Magnetic polarity detection Filter elimination

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