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Efficacy of Dangua Fang(丹瓜方) on endothelial cells damaged by oxidative stress

Journal of Traditional Chinese Medicine 2022年第6期42卷 900-907页

作者： HENG Xianpei LI Liang YANG Liuqin WANG Zhita Department of Endocrinology People's Hospital Affiliated to Fujian University of Traditional Chinese MedicineFuzhou 350004China

OBJECTIVE: To evaluate the protective effects of serum containing Dangua Fang( 丹瓜方) on vascular endothelium damaged by oxidative stress. METHODS: Five experiments were completed in this paper. In the first experiment, we found the most suitable serum containing Dangua Fang by comparing groups with different serum containing Dangua Fang. In the second experiments we analyzed Dangua Fang influencing endothelial cell viability and apoptosis and cell cycle. The third experiment on Dangua Fang intervention of mitochondrial respiratory chain. The fourth experiment on Dangua Fang intervention of mitochondrial membrane potential. And finally, on the fifth experiment we researched the mechanism of Dangua Fang improving mitochondrial function by comparing the Na~+-k~+-ATPase and peroxisome proliferator-activated receptor-gamma coactivator-1alpha(PGC-1α) in the Dangua group with the diazoxide group and Co Q+Vit C group. RESULTS: We compared the control group in the first experiments and the OD values in DZ1 group was the most significant in all intervening groups. The recipe of DZ1(5% serum containing Dangua Fang) was used in the following experiments. Compared with the control group, cell viability, cell cycle(G2 + S), cytochrome c oxidase(COX), R3 red/green, R2 red/green, R1 red/green decreased and apoptosis, succinate dehydrogenase(SDH), green(R2 + R3), Na+-k+-ATPase, PGC-1α increased in the model group. Compared with the model group, cell viability, G2+S, COX, R3 red/green, R2 red/green, R1 red/green raised and apoptosis, green(R2 + R3), Na-K-ATPase decreased in the Dangua group;G2 + S, R3 red/green, R2 red/green, R1 red/green raised and green(R2 + R3) decreased in the Co Q + Vit C group. Na-K-ATPase increased in the combined group(P < 0.05 or < 0.01). CONCLUSIONS: Dangua Fang protects oxidative stressinduced endothelial cells damaged by promotion of mitochondrial biogenesis, reduction of Na~+-K~+-ATPase activity and regulation of mitochondrial respiratory chain function restoring mitochondrial membrane potential.

关键词： mitochondria oxidative stress endothelial cells peroxisome proliferator-activated receptor gamma coactivator 1-alpha Dangua Fang

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衡先培治疗糖尿病便秘经验

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中医药临床杂志 2018年第5期30卷 857-859页

作者：丁香衡先培福建中医药大学福建福州350108 福建中医药大学附属人民医院福建福州350004

便秘是糖尿病慢性并发症之一,且多见于年龄较大者,不仅影响患者生活质量和情绪,且使得热量无出路,影响降血糖药物的作用。衡先培教授认为,其病因病机以脾肾功能失调、气虚津亏为本,气滞痰瘀互结为标。治疗上,衡老师认为不能专顾泻下通下... 详细信息

便秘是糖尿病慢性并发症之一,且多见于年龄较大者,不仅影响患者生活质量和情绪,且使得热量无出路,影响降血糖药物的作用。衡先培教授认为,其病因病机以脾肾功能失调、气虚津亏为本,气滞痰瘀互结为标。治疗上,衡老师认为不能专顾泻下通下,而应辨清病因,根据病因不同,审因论治,辨证治之。

关键词：衡先培教授糖尿病便秘经验

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Dangua Fang(丹瓜方)regulating tricarboxylic acid cycle and respiratory chain and its mechanism in diabetic rats

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Journal of Traditional Chinese Medicine 2023年第6期43卷 1150-1159页

作者： HENG Xianpei WANG Zhita YANG Liuqing LI Liang HUANG Suping Department of Endocrinology People's Hospital Affiliated to Fujian University of Traditional Chinese MedicineFuzhou 350004China Academy of Integrative Medicine Fujian Fujian University of Traditional Chinese MedicineFuzhou 350122China

OBJECTIVE:To investigate the influence and possible targets of Dangua Fang(丹瓜方)on tricarboxylic acid(TCA)cycle and respiratory chain to enrich the prescription’s mechanism of effective intervention on glycolipid metabolic diseases such as type 2 diabetes.METHODS:After interventional rats were fed with high glucose and high fat diet ad libitum for 4 weeks,intraperitoneally injected streptozotocin to induce diabetic model.According to blood glucose level,28 diabetic rats were selected and continued to be fed with high glucose and high fat diet,were stratified by body weight,and divided randomly by blood glucose into Model group(was given sterile water by gastric perfusion and injected aquae pro injection intraperitoneally),Dangua group[Dangua liquor(丹瓜方液)20.5 g·kg^(-1)·d^(-1) by perfusion and aquae pro injection intraperitoneally],Inhibitor group[sterile water by perfusion and nicotinamide phosphoribosyl transferase(Nampt)specific blocker GEN-6171.25 mg/kg intraperitoneally],DanInhit group(Dangua liquor and GEN-617 synchronously).Control group were continuously fed with ordinary diet.The intervention was last for 10 weeks.Body weight(BW),liver index(LI),glycosylated hemoglobin(HbA1c),TC,TG,free fatty acids(FFA),creatinine(Cr),and A-ketoglutarate(α-KG),Iso-citric acid(ICA),oxaloacetic acid(OAA)were tested.The cytochrome C oxidase(COX)and Succinate dehydrogenase(SDH)were evaluated by Colorimetry;Nampt protein,Adenosine triphosphate(ATP)synthase(ATPs),Nicotinamide adenine dinucleotide(NAD^(+))and its reduced(NADH)in liver were measured by enzyme linked immunosorbent assay.The expressions of Nampt and mitochondrialnadhdehydrogenase-1(mt-ND1)gene in liver was assessed by real-time polymerase chain reaction.Hepatic tissue staining was also completed.RESULTS:The levels of BW,ICA,α-KG and Nampt-mRNA in the Model group are lower than that in the Normal group(P<0.05),conversely,liver weight,LI,TC,HbA1c,SDH and ATPs,mt-ND1-mRNA,and Nampt protein in the Model group are higher(P<0.01,P<0.05).Compared with Model group,the levels

关键词： Dangua Fang diabetes mellitus citric acid cycle electron transport nicotinamide phosphoribosyltransferase

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衡先培应用活血化瘀法治疗糖尿病临床经验

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中医药临床杂志 2019年第7期31卷 1247-1250页

作者：阮艳艳衡先培福建中医药大学

糖尿病一般认识从消渴阴虚燥热论治。但其发生、发展、变化过程与瘀血紧密相关。衡先培教授认为,口干是消渴的代表性临床特征,古代医籍中不少关于口干的论述都与消渴有关。瘀血既可以是消渴的病因,也可以是消渴进展所导致的病理改变。... 详细信息

糖尿病一般认识从消渴阴虚燥热论治。但其发生、发展、变化过程与瘀血紧密相关。衡先培教授认为,口干是消渴的代表性临床特征,古代医籍中不少关于口干的论述都与消渴有关。瘀血既可以是消渴的病因,也可以是消渴进展所导致的病理改变。临床实践中瘀血可与多种病机同时并存,治疗也当标本两兼。衡教授常用联合治法包括活血化瘀法,清热生津、活血化瘀,益气健脾、活血化瘀,益精补肾、活血化瘀,化痰祛湿、活血化瘀。临床疗效显著提高。糖尿病以气阴两虚为本,气虚血瘀或阴虚血瘀;脾虚失运,水湿内生,聚而为痰,日久痰阻血瘀。提出应该将活血化瘀法贯穿糖尿病治疗的由始至终。

关键词：糖尿病活血化瘀临床经验

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Mechanisms of Dangua Fang(丹瓜方)in multi-target and multi-method regulation of glycolipid metabolism based on phosphoproteomics

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Journal of Traditional Chinese Medicine 2024年第2期44卷 334-344页

作者： HENG Xianpei WANG Zhita LI Liang YANG Liuqing HUANG Suping JIN Lang HE Weidong Department of Endocrinology People's Hospital Affiliated to Fujian University of Traditional Chinese MedicineFuzhou 350004China Academy of Integrative Medicine Fujian University of Traditional Chinese MedicineFuzhou 350122China Faculty of Humanities and Management Fujian University of Traditional Chinese MedicineFuzhou 350122China Department of Geriatrics People's Hospital Affiliated to Fujian University of Traditional Chinese MedicineFuzhou 350004China

OBJECTIVE:To explore the mechanism of Dangua Fang(丹瓜方,DGR)in multi-target and multi-method regulation of glycolipid metabolism based on phosphoproteomics.METHODS:Sprague-Dawley rats with normal glucose levels were randomly divided into three groups,including a conventional diet control group(Group A),high-fat-highsugar diet model group(Group B),and DGR group(Group C,high-fat-high-sugar diet containing 20.5 g DGR).After 10 weeks of intervention,the fasting blood glucose(FBG),2 h blood glucose[PBG;using the oral glucose tolerance test(OGTT)],hemoglobin A1c(HbA1c),plasma total cholesterol(TC),and triglycerides(TG)were tested,and the livers of rats were removed to calculate the liver index.Then,hepatic portal TG were tested using the Gross permanent optimization-participatiory action planning enzymatic method and phosphoproteomics was performed using liquid chromatography with tandem mass spectrometry(LC-MS/MS)analysis followed by database search and bioinformatics analysis.Finally,cell experiments were used to verify the results of phosphoproteomics.Phosphorylated mitogen-activated protein kinase kinase kinase kinase 4(MAP4k4)and phosphorylated adducin 1(ADD1)were detected using western blotting.RESULTS:DGR effectively reduced PBG,TG,and the liver index(P1.2),DGR upregulated the modification of 228 phosphorylation sites involving 204 corresponding function proteins,and downregulated the modification of 358 phosphorylation sites involving 358 corresponding function proteins,which included correcting 75 phosphorylation sites involving

关键词： phosphoproteomics glycolipid metabolism therapeutic mechanism compound formula Dangua Fang

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衡先培教授治疗糖尿病人尿液浑浊临床经验

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辽宁中医药大学学报 2015年第11期17卷 192-194页

作者：刘冲衡先培福建中医药大学福建福州350122 福建中医药大学附属人民医院福建福州350004

尿液浑浊是糖尿病病人常见的伴随症状之一,严重影响病人的心理、生理健康,但目前尚无系统的治疗方法。衡先培教授认为尿液浑浊属水液代谢失常的病症,肾是致病的关键脏腑,且涉及到肝、肺、脾等脏,并在此基础上进行辨证论治,认为"恢复肾... 详细信息

尿液浑浊是糖尿病病人常见的伴随症状之一,严重影响病人的心理、生理健康,但目前尚无系统的治疗方法。衡先培教授认为尿液浑浊属水液代谢失常的病症,肾是致病的关键脏腑,且涉及到肝、肺、脾等脏,并在此基础上进行辨证论治,认为"恢复肾气、清肺生津、运脾化热、疏理肝气"是其治疗大法,最后将临床常用药物略举一二。

关键词：衡先培糖尿病尿液浑浊病因病机治疗思路

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全域旅游背景下河流生态廊道规划设计

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水利技术监督 2022年第4期30卷 97-100页

作者：衡先培北京土人城市规划设计股份有限公司北京海淀100871

文章通过河道生态修复、滨河慢道系统构建、休闲旅游功能提升等策略,打造一条集生态保护、休闲旅游、文化体验、滨水生活于一体的百里丰溪绿廊。丰溪河慢生活走廊是践行“两山”理论,打造广丰样本的关键举措;实施乡村振兴,统筹城乡发展... 详细信息

文章通过河道生态修复、滨河慢道系统构建、休闲旅游功能提升等策略,打造一条集生态保护、休闲旅游、文化体验、滨水生活于一体的百里丰溪绿廊。丰溪河慢生活走廊是践行“两山”理论,打造广丰样本的关键举措;实施乡村振兴,统筹城乡发展的内在要求;促进全域旅游、城乡高品质发展的有力抓手。

关键词：全域旅游河流生态廊道生态修复

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Study on Dangua recipe regulating miR-34a/Nampt Axis in diabetic rats

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中国医学文摘:内科学分册(英文版) 2023年第2期40卷 74-75页

作者：衡先培 Dept Endocrinol People's HospFujian TCM UnivFuzhou 350004

Objective To study the effect of Dangua Recipe regulating microRNA(miR)-34a/Nampt niacin nicotinate phosphoribosyl transferase(Nampt)axis controlling glycolipid metabolism.Methods After being raised for 4 weeks with h... 详细信息

关键词： Nampt rats sugar

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