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Genome sequence of Ralstonia solanacearum species complex phylotype Ⅰ strain RSCM isolated from Cucurbita maxima

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Genome sequence of Ralstonia solanacearum species complex ph...

创新植保科技,服务南粤大地——广东省农业科学院植物保护研究所建所五十周年记

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： She Xiaoman Lan Guobing Yu Lin Tang Yafei Li Zhenggang he zifu Guangdong Provincial Key Laboratory of High Technology for Plant Protection Plant Protection Research Institute Guangdong Academy of Agriculture Sciences

Ralstonia solanacearum species complex is a devastating phytopathogen with an unusually wide host range,and new host plants are continuously being *** June 2016,a new bacterial wilt on Cucurbita maxima(RSCM) was firstly observed in Guangdong ***,the complete genome of RSCM was sequenced and ***-genome sequencing was performed on the Illumina HiSeq 2500-PE 125 platform with MPS Illumina *** assembly is 6 000 712 bp in size with a GC content of 66.69%,consisting of a circular chromosome(3 788 542 bp;GC%:66.47%) and a megaplasmid(2 212 170 bp;GC%:67.07%).None additional plasmids were found.A total of 5 404 CDS were *** genome includes 59 interspersed repeated sequences and 596 tandem repeated *** RSCM genome contained 12 rRNAs(9 rRNAs are located on the chromosome and 3 rRNAs are located on the megaplasmid) and 58 tRNAs(40 tRNAs are located on the chromosome and18 tRNAs are located on the mega-plasmid).A total of 4 914 proteins were successful classified into 25 COG functional categories,while a total of 2 346 proteins have KEGG orthologs.

关键词： Ralstonia solanacearum species complex Cucurbita maxima Genome sequencing

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植物保护学报 2023年第5期50卷 1109-1112页

作者：何自福高燕隋晶晶广东省农业科学院植物保护研究所农业农村部华南果蔬绿色防控重点实验室广东省植物保护新技术重点实验室广州510640

广东省农业科学院植物保护研究所成立于1973年8月,是广东省农业科学院下属15个研究机构之一,为省属公益一类事业单位。前身可追溯到1950年1月成立的华南区农业推广繁殖站石牌试验场病虫害组及1960年1月成立的广东省农业科学院植物保护... 详细信息

广东省农业科学院植物保护研究所成立于1973年8月,是广东省农业科学院下属15个研究机构之一,为省属公益一类事业单位。前身可追溯到1950年1月成立的华南区农业推广繁殖站石牌试验场病虫害组及1960年1月成立的广东省农业科学院植物保护系。在50年的发展历程中,研究所持续为广东省农业生产安全和农作物健康保驾护航。

关键词：植物保护研究植保科技农业推广农业生产安全广东省农业科学院华南区发展历程

Transcriptome and Metabolome Analyses Reveal Jasmonic Acids May Facilitate the Infection of Cucumber Green Mottle Mosaic Virus in Bottle Gourd

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Transcriptome and Metabolome Analyses Reveal Jasmonic Acids ...

Identification and Genomic Characterization of Ralstonia pseudosolanacearum Strains Isolated from Pepino Melon in China

中国植物病理学会2023年学术年会

作者： LI Zhenggang TANG Yafei YU Lin LAN Guobing DING Shanwen She Xiaoman he zifu Guangdong Provincial Key Laboratory of High Technology for Plant Protection Plant Protection Research Institute Guangdong Academy of Agricultural Sciences

CGMMV is a typical seed-borne tobamovirus that mainly infects cucurbit *** to the rapid growth of international trade, CGMMV has spread worldwide and become a significant threat to cucurbit *** various studies focusing on the interaction between CGMMV and host plants,the molecular mechanism of CGMMV infection is still *** this study, we utilized transcriptome and metabolome analyses to investigate the anti-viral response of bottle gourd(Lagenaria siceraria) under CGMMV *** transcriptome analysis revealed that in comparison to mock-inoculated bottle gourd, 1929 differently expressed genes( DEGs) were identified in CGMMV-inoculated bottle *** them, 1397 genes were upregulated while 532 genes were *** pathway enrichment indicated that the DEGs were involved in metabolic, biosynthesis of secondary metabolites,plant hormone signal transduction, plant-pathogen interaction, and starch and sucrose metabolism *** metabolome result showed there were 76 differentially accumulated metabolites( DAMs), of which 69 metabolites were up-accumulated, and 7 metabolites were *** DAMs were clustered into several pathways, including biosynthesis of secondary metabolites, tyrosine metabolism, flavonoid biosynthesis, carbon metabolism, and plant hormone signal transduction *** the transcriptome and metabolome results, we further investigate the plant hormone signal transduction pathway, especially the jasmonic acids( JAs) *** with the mock treatment, the genes and metabolites involved in JAs synthesis pathway were induced upon CGMMV ***, most of the transcription factors( TFs) related with JAs signaling pathway were also ***, silencing of A OS gene, which is the key gene involved in JAs synthesis,reduced CGMMV *** findings suggest that JAs may facilitate CGMMV infection in bottle gourd.

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Identification and Genomic Characterization of Ralstonia pse...

我国46个棉花品种对木尔坦棉花曲叶病毒的抗性鉴定

中国植物病理学会2023年学术年会

作者： DING Shanwen YU Lin LAN Guobing TANG Yafei LI Zhenggang he zifu She Xiaoman Guangdong Provincial Key Laboratory of High Technology for Plant Protection Plant Protection Research Institute Guangdong Academy of Agricultural Sciences

The Ralstonia solanacearum species complex( RSSC) strains are severely destructive on a wide host range, and caused producing serious economic *** 2018, a bacterial wilt on pepino melon was observed in Huizhou city, Guangdong province, *** the pathogen of the pepino melon bacterial wilt and exploring its pathogenesis might be useful in developing control *** and biochemical, phylotyping, and pathogenicity were carried *** isolates of pepino melon were identified as Ralstonia pseudosolanacearum( *** phylotype I), race1, biovar 3.A strain RS24 has a genome of 6.10 Mb that consists of a 3 908 757 bp circular chromosome, a 2 077 399 bp circular megaplasmid and a 118 798 bp circular small plasmid with 5 217 protein-coding genes, 60 transfer RNAs, 5 non-coding RNAs, and 4 rRNAs(5 S, 16 S, and 23 S),and the G + C content of 66.69%.Functional annotation successfully classified 4 026 genes into 25 COG *** candidate CRISPRs, 77 genomic islands and 10 prophages were ***24 contained a small plasmid carrying type Ⅳ secretion system(T4 SS), and was different from GMI1000 in the number and type of T3 SS effectors and the gene encoding T6 *** genome sequencing and analysis of strain RS24 provided effective reference data for further study of the pathogenic mechanism and exploring the host specificity of *** was the first record of pepino melon bacterial wilt caused by ***.

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植物保护学报 2017年第4期44卷 601-608页

作者：汤亚飞何自福佘小漫蓝国兵广东省农业科学院植物保护研究所广东省植物保护新技术重点实验室广州510640

为明确我国主要棉花品种对木尔坦棉花曲叶病毒(Cotton leaf curl Multan virus,CLCuMuV)的抗性水平,采用烟粉虱田间自然传毒和CLCuMuV侵染性克隆人工接种方法分别对收集的46个棉花品种进行抗性鉴定。结果显示:46个棉花品种田间播种后90... 详细信息

为明确我国主要棉花品种对木尔坦棉花曲叶病毒(Cotton leaf curl Multan virus,CLCuMuV)的抗性水平,采用烟粉虱田间自然传毒和CLCuMuV侵染性克隆人工接种方法分别对收集的46个棉花品种进行抗性鉴定。结果显示:46个棉花品种田间播种后90 d,新海21号、新海31号、新37号及112-2号4个品种自然发病的病株率为41.70%~100.00%,病情指数为39.81~100.00;新陆早36号、新陆中52号、中棉35号、中棉43号、鲁棉研36号、金宏祥10号6个品种的病株率为8.30%~16.70%,病情指数为0.93~9.26;其余品种未见发病。46个棉花品种人工接种CLCuMuV后60 d,新海21号、新海31号、新海37号和112-2号4个品种的病情指数为75.00~100.00,表现为高感;新陆中66号和新陆早46号病情指数分别为20.83和20.37,表现为中感;新陆早62号等10个品种的病情指数为10.46~15.87,表现为中抗;新陆早47号等14个品种的病情指数为5.26~9.26,表现为抗病;豫棉15号等11个品种的病情指数为2.22~4.86,表现为高抗;新陆早13号、新陆早61号、新陆中32号、新陆中56号和中棉41号病情指数均为0,表现为免疫。

关键词：棉花品种木尔坦棉花曲叶病毒侵染性克隆农杆菌注射接种抗性鉴定

广东黄秋葵黄脉曲叶病样中检测到烟粉虱传双生病毒

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植物保护 2010年第1期36卷 65-68页

作者：董迪何自福柴兆祥甘肃农业大学草业学院兰州730070 广东省农业科学院植物保护研究所广州510640

黄秋葵是近几年来从日本和我国台湾引进的一种蔬菜作物。近期,广东的黄秋葵上发生了黄脉曲叶病。病株的典型症状表现为叶脉黄化,在叶片正面形成网络状,在叶背面叶脉肿大突起明显,病株幼叶小且向下卷曲,甚至整片幼叶黄化。植株早期被感... 详细信息

黄秋葵是近几年来从日本和我国台湾引进的一种蔬菜作物。近期,广东的黄秋葵上发生了黄脉曲叶病。病株的典型症状表现为叶脉黄化,在叶片正面形成网络状,在叶背面叶脉肿大突起明显,病株幼叶小且向下卷曲,甚至整片幼叶黄化。植株早期被感染表现矮化。在发生黄脉曲叶病的黄秋葵田间,其病株率高达60%以上。用烟粉虱传双生病毒简并引物对随机采集的病样进行PCR检测,从这些病样中均能扩增出1条预期大小为570 bp的特异片段;基因克隆及测序分析结果表明,与该特异片段同源的均属双生病毒科菜豆金色花叶病毒属病毒DNA,其中与木尔坦棉花曲叶病毒(Cotton leaf curl Multan virus,CLCuMV)分离物G6相似性最高,为99%。这些研究结果表明,广东黄秋葵黄脉曲叶病中存在烟粉虱传双生病毒,该病害可能也是由CLCuMV侵染引起的。

关键词：黄秋葵黄脉曲叶病烟粉虱传双生病毒木尔坦棉花曲叶病毒 PCR检测

An Improved Practical Byzantine Fault-Tolerant Algorithm Based on XGBoost Grouping for Consortium Chains

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Computers, Materials & Continua 2025年第1期82卷 1295-1311页

作者： Xiaowei Wang Haiyang Zhang Jiasheng Zhang Yingkai Ge Kexin Cui zifu Peng Zhengyi Li Lihua Wang College of Electronic and Information Engineering Shandong University of Science and TechnologyQingdao266590China

In response to the challenges presented by the unreliable identity of the master node,high communication overhead,and limited network support size within the Practical Byzantine Fault-Tolerant(PBFT)algorithm for consortium chains,we propose an improved PBFT algorithm based on XGBoost grouping called XG-PBFT in this ***-PBFT constructs a dataset by training important parameters that affect node performance,which are used as classification indexes for *** XGBoost algorithm then is employed to train the dataset,and nodes joining the system will be grouped according to the trained grouping *** them,the nodes with higher parameter indexes will be assigned to the consensus group to participate in the consensus,and the rest of the nodes will be assigned to the general group to receive the consensus *** order to reduce the resource waste of the system,XG-PBFT optimizes the consensus protocol for the problem of high complexity of PBFT ***,we evaluate the performance of *** experimental results show that XG-PBFT can significantly improve the performance of throughput,consensus delay and communication complexity compared to the original PBFT algorithm,and the performance enhancement is significant compared to other algorithms in the case of a larger number of *** results demonstrate that the XG-PBFT algorithm is more suitable for large-scale consortium chains.

关键词： Consortium chain PBFT grouping reputation XGBoost

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侵染广东番茄的番茄褪绿病毒分子鉴定

植物保护 2017年第2期43卷 133-137页

作者：汤亚飞何自福佘小漫蓝国兵广东省农业科学院植物保护研究所广州510640 广东省植物保护新技术重点实验室广州510640

在广东番茄上发现一种新病害,病株表现为叶片褪绿,叶脉颜色变深及叶片增厚等症状。利用番茄褪绿病毒Tomato chlorosis virus(ToCV)HSP70基因的两对特异引物对番茄病样进行RT-PCR检测,结果表明,从所采集的6份病样中均扩增到预期大小的DN... 详细信息

在广东番茄上发现一种新病害,病株表现为叶片褪绿,叶脉颜色变深及叶片增厚等症状。利用番茄褪绿病毒Tomato chlorosis virus(ToCV)HSP70基因的两对特异引物对番茄病样进行RT-PCR检测,结果表明,从所采集的6份病样中均扩增到预期大小的DNA特异片段。对其中1份样品的扩增片段进行克隆与序列分析,结果表明,扩增片段包括1个长度为1 665个核苷酸的完整病毒基因,其核苷酸序列与已报道的ToCV HSP70基因有较高的同源性,表明广东番茄受到了ToCV的侵染。但ToCV广东番茄分离物的HSP70序列与国内外已报道的各分离物的同源性均低于82%,存在较大差异,其中与塞浦路斯tomato、约旦JU_20分离物的同源性最高,为81.8%。这是ToCV在广东发生的首次报道,也是该病毒HSP70基因序列存在显著差异分离物的首次发现。

关键词：番茄病毒病番茄褪绿病毒 HSP70基因

香葱伴生番茄对青枯病及根际微生态特征的影响

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广东农业科学 2024年第1期51卷 10-20页

作者：佘小漫姚挺黄真珍何自福广东省农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点试验室/广东省植物保护新技术重点试验室广东广州510640 广东省农业技术推广中心广东广州510145

【目的】为明确香葱伴生对番茄青枯病及根际微生态特征的影响,揭示香葱伴生番茄降低青枯病发病率的机理。【方法】通过田间试验设置番茄单作和香葱伴生番茄2个处理,分别采集单作发病番茄(QK)、单作健康番茄(NBS)和香葱伴生番茄(BS)的根... 详细信息

【目的】为明确香葱伴生对番茄青枯病及根际微生态特征的影响,揭示香葱伴生番茄降低青枯病发病率的机理。【方法】通过田间试验设置番茄单作和香葱伴生番茄2个处理,分别采集单作发病番茄(QK)、单作健康番茄(NBS)和香葱伴生番茄(BS)的根际土壤,应用16S rDNA高通量测序和液相色谱-质谱联用(LC-MS)技术,探究香葱伴生对番茄根际微生态特征的影响。【结果】田间试验结果表明,番茄单作处理和香葱伴生番茄处理的青枯病平均病株率分别为50.48、31.43%,香葱伴生番茄处理对番茄青枯病的防治效果为37.74%。16S rDNA高通量测序结果表明,BS、NBS根际土壤细菌群落相对丰度和多样性均显著高于QK;与QK相比,BS和NBS根际土壤细菌茄科雷尔氏菌属相对丰度显著降低,而芽孢杆菌属相对丰度升高;与NBS相比,BS根际土壤细菌茄科雷尔氏菌属相对丰度降低,芽孢杆菌属相对丰度显著提高。利用LC-MS技术共鉴定出番茄根际土壤代谢物586种,其中正离子模式代谢物320种,负离子模式代谢物266种,并将代谢物的功能注释至新陈代谢、遗传信息加工和环境信息加工等代谢通路;与NBS相比,BS根际土壤代谢物中的缬氨酸、L-天冬酰胺和柠檬酸的含量均显著降低,L-赖氨酸、谷氨酸、D-谷氨酰胺和异亮氨酸的含量也低于NBS,但差异未达到显著水平。【结论】香葱伴生番茄显著降低番茄青枯病田间发病率,改变了番茄根际土壤中细菌群落结构和番茄根际土壤代谢物含量。

关键词：香葱伴生番茄青枯病根际土壤细菌根际土壤代谢物防效

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