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Microwave/UV-Vis/H2O2 Process for environmental Remediation:Elimination of Phototransformation of Malachite Green(MG) into Leuco-Malachite Green(LMG)

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Microwave/UV-Vis/H2O2 Process for Environmental Remediation:...

Microbial PAH-Degradation in Soil: Degradation Pathways and Contributing Factors

第五届全国环境化学大会

作者： yongming ju shaogui yang cheng sun~* (state key laboratory of pollution control and resource reuse,school of the environment, nanjing university,nanjing 210093,P.R.china

Phototransformations of malachite green(MG) into leuco-malachite green(LMG) with UV-Vis irradiation from microwave-powered electrodeless discharge lamps (EDL) during MW/UV-Vis and MW/UV-Vis/HO process were comparatively *** gain insight about the degradation courses during MW/UV-Vis-based and MW/UV-Vis/HO-based processes,high performance liquid

关键词： Microwave/UV-Vis/H2O2 phototransformation malachite green(MG) Leuco-Malachite Green(LMG)

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Pedosphere 2006年第5期16卷 555-565页

作者： ZHANG Xu-Xiang cheng Shu-Pei ZHU cheng-jun sun Shi-Lei state key laboratory of pollution control and resource reuse of china Department of Environmental Sciences Nanjing University Nanjing 210093 （China）

Adverse effects on the environment and high persistence in the microbial degradation and environmental fate of polycyclic aromatic hydrocarbons (PAHs) are motivating interest. Many soil microorganisms can degrade PAHs and use various metabolic pathways to do so. However, both the physio-chemical characteristics of compounds as well as the physical, chemical, and biological properties of soils can drastically influence the degradation capacity of naturally occurring microorganisms for field bioremediation. Modern biological techniques have been widely used to promote the efficiency of microbial PAH-degradation and make the biodegradation metabolic pathways more clear. In this review microbial degradation of PAHs in soil is discussed, with emphasis placed on the main degradation pathways and the environmental factors affecting biodegradation.

关键词： bioremediation microbial degradation PAHs soil

A systematic understanding of microbial reductive dechlorination towards an improved “one health” soil bioremediation: A review and perspective

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Science china.Technological Sciences 2024年第10期67卷 3009-3031页

作者： he Yan LI ShuYao YUAN Jing cheng Jie DOU JiBo yang XueLing XU JianMing Zhejiang Provincial key laboratory of Agricultural resources and environment Institute of Soil and Water Resources and Environmental ScienceCollege of Environmental and Resource SciencesZhejiang University key laboratory of environment Remediation and Ecological health Ministry of Education Microbiome Network and Department of Agricultural Biology Colorado State University Departments of Chemistry and Chemical & Biomolecular Engineering Institute for Functional Intelligent Materials (I-FIM)National University of Singapore

Chlorinated organic pollutants(COPs), both emerging and traditional, are typical persistent pollutants that harm soil health worldwide. Dechlorinators mediated reductive dechlorination is the optimal way to completely remove COPs from anaerobic soil through a redox reaction driven by electron transfer during microbial anaerobic respiration. Generally, the dechlorinated depletion of COPs in situ often interacts with multiple element biogeochemical activities, e.g., methanogenesis, sulfate reduction, iron reduction, and denitrification. Elucidating the relevance of biogeochemical cycles between COPs and multiple elements and the coupled mechanisms involved, thus, helps to develop effective pollution control strategies with the balance between pollution degradation and element cycles in heterogeneous soil, ultimately contributing to “one health” goal. In this review, we summarized the microbial-chemical coupling redox processes and the driving factors, elucidated the interspecies metabolites exchange and electron transfer mechanisms within COP-dechlorinating communities, and further proposed a detailed design, construction, and analysis framework of engineering COP-dechlorinating microbiomes via “top-down” selfassembly and “bottom-up” synthesis to pave the way from laboratory to practical field application. Especially, we delve into the major challenges and perspectives surrounding the design of state-of-the-art synthetic microbial communities. Our goal is to improve the understanding of the microbial-mediated coupling between reductive dechlorination and element biogeochemical cycling, with a particular focus on the implications for health-integrated soil bioremediation under the “one health” concept.

关键词： reductive dechlorination biogeochemical redox processes interspecies interaction synthetic microbiomes, anaerobic soils

Bioaccumulation,Depuration and Biotransformation of 4,4'-dibromodiphenyl ether(BDE 15) in freshwater fish Crucian carp(Carassius auratus)

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Bioaccumulation,Depuration and Biotransformation of 4,4'-dib...

Adsorption of Pb2+ from water by new chelating fiber

中国毒理学会环境与生态毒理学专业委员会第二届学术研讨会暨中国环境科学学会环境标准与基准专业委员会2011年学术研讨会

作者： Jie cheng,Zhigang Zhao,Mengnan Shen,Shixiang Gao~* state key laboratory of pollution control and resource reuse,school of the environment,nanjing university,nanjing 210046,PR china

Polybrominated diphenyl ethers(PBDEs) are a class of flame retardants that known to have bioaccumulation and potential toxicity to *** PBDEs in animals have been studied in many previous researches,little is known about the single low brominated diphenylethers of their fate in the aquatic *** the present study,we investigated the tissue distribution,excretion, depuration,and potential biotransformed products of 4,4’-dibromodiphenyl ether(BDE 15) after water exposure using freshwater fish Crucian carp(Carassius auratus).Fish were exposed to BDE 15 at different concentrations for 50 days,followed by a 14-day depuration period,to examine the levels of BDE 15 in the muscle,gill,liver and gut content *** analyses,bioaccumulation parameters, such as rate constants of uptake(k) and depuration(k),and corresponding half-life(t) were also *** showed that obviously rapid uptake and elimination of BDE 15 by carps was observed in both treated groups,and BDE 15 was most concentrated in the gill and liver to reach the peak concentrations on day *** corresponding estimated bioconcentration factor(BCF) values obtained for liver and gill in the low-dose treatment(1.28×10 and 1.69×10 dry weight,respectively) were higher than for ***,five unknown compounds were detected as BDE 15 metabolites in the exposed fish,a novel finding which indicated that debromination of BDEs was not the only metabolic pathway observed in *** 4-bromodiphenyl ether and diphenyl ether, another three products were proposed tentatively as mono-OH-BDE 15,diOH-BDE 15 and bromophenol using GC/EI-MS/*** the best of our knowledge,it is the first indications for the oxidation metabolism products of PBDEs in vivo exposed freshwater *** results will be useful for determination of the origin of OH-PBDEs present in wild aquatic organisms.

关键词： BDE 15 bioaccumulation parameters metabolism OH-PBDEs Crucian carp

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Adsorption of Pb2+ from water by new chelating fiber

酿酒酵母与球形红假单胞菌原生质体跨界融合研究

持久性有机污染物论坛2012暨第七届持久性有机污染物全国学术研讨会

作者： Xin yang 1 ,Jinnan Wang 1, 2 ,cheng cheng 1 ,Aimin Li 1, 2 1 state key laboratory of pollution control and resource reuse & school of the environment 2 nanjing university, nanjing, china. 210046

*** The discharge of lead into the environment is a serious pollution problem affecting water quality. Long-term drink of water at high lead level will cause anemia, headache, chills, diarrhea and poisoning leading to the dysfunction of kidneys, reproductive system, liver, brain and central nervous system. Thus, lead had been a serious public health issue worldwide.

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生物工程学报 1996年第S1期12卷 52-57页

作者：王玉水邹贵华崔益斌程树培南京大学环境科学与工程系

测定了球形红假单胞菌原核细胞与酿酒酵母真核细胞的原生质体形成、再生及融合最佳组合条件。以溶菌酶ＥＤＴＡ反应系统处理球形红假单胞菌Ｐ９４７９，最适脱壁条件为：溶菌酶浓度０５ｍｇ／ｍｌ，ＥＤＴＡ浓度为０２％，蔗糖浓... 详细信息

测定了球形红假单胞菌原核细胞与酿酒酵母真核细胞的原生质体形成、再生及融合最佳组合条件。以溶菌酶ＥＤＴＡ反应系统处理球形红假单胞菌Ｐ９４７９，最适脱壁条件为：溶菌酶浓度０５ｍｇ／ｍｌ，ＥＤＴＡ浓度为０２％，蔗糖浓度２０％，酶作用时间为４０ｍｉｎ；此条件下原生质体形率为７８９％，再生率为１１２％。用蜗牛酶巯基乙醇反应系统处理酿酒酵母Ｙ９４０７，最适脱壁条件为：蜗牛酶浓度１０％，巯基乙醇浓度０１％，蔗糖浓度２０％，酶作用时间为３０ｍｉｎ；此条件下酵母原生质体形成率为９９８％，再生率为９７％。用聚乙二醇诱导Ｐ９４７９与Ｙ９４０７的原生质体发生融合，当聚乙二醇的浓度为３０％，Ｃａ２＋浓度为５０ｍｍｏｌ／Ｌ，ｐＨ为６５，时间为１０ｍｉｎ时，有最高的融合率为７６×１０－６。

关键词：酿酒酵母球形红假单胞菌跨界融合最佳条件

Preparation of hapten-specific monoclonal antibody for cadmium and its ELISA application to aqueous samples

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Frontiers of environmental Science & Engineering 2011年第3期5卷 409-416页

作者： Huan he Bo TANG cheng sun shaogui yang Weijuan ZheNG Zichun HUA The state key laboratory of pollution control and resource reuse School of the EnvironmentNanjing UniversityNanjing 210093China The state key laboratory of Pharmaceutical Biotechnology School of Life ScienceNanjing UniversityNanjing 210093China

High-affinity and specific monoclonal antibodies against cadmium-ethylene diamine tetraacetic acid(EDTA)complex have been produced using the hybridoma technique.A hapten was synthesized and characterized by Fourier Transform Infrared Spectroscopy(FT-IR)and *** enzyme-linked immunosorbent assay(ELISA)for quantitative detection of cadmium in aqueous sample was *** monoclonal antibody with high level of binding affinity for Cd-IEDTA-BSA and high specificity for soluble Cd-EDTA complex showed less than 0.99%cross-reactivity with other 11 *** limit of detection was 0.10μg·L^(-1),and the effective linear range was 10^(-1)-10^(3)μg·L^(-1).The intra-and inter-assay coefficient variations were 1.5%-6.3%and 3.2%-7.4%,*** spike recovery in different water samples were between 98.5%and 110.3%.The detection limit of this assay was well below the allowable concentration of cadmium(3μg·L^(-1)),and the working range was wider than that of other methods which showed the range of 2.19-86.38 and 0-10^(3)μg·L^(-1).The competitive ELISA established in this paper was sensitive and accurate in the screening of cadmium in aqueous *** results will lay a solid foundation for construction of an immunoassay kit for cadmium.

关键词： cadmium hapten monoclonal antibody enzyme-linked immunosorbent assay(ELISA)

Nitrated and parent PAHs in the surface water of Lake Taihu,china.Occurrence,distribution,source,and human health risk assessment

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Journal of environmental Sciences 2021年第4期33卷 159-169页

作者： Jijie Kong Yuxuan Dai Mengshu Han Huan he Jiapeng Hu junyi Zhang junzhe Shi Qiming Xian shaogui yang cheng sun The state key laboratory of pollution control and resource reuse School of the EnvironmentNanjing UniversityNanjing 210023China school of environment Nanjing Normal UniversityNanjing 210023China College of Ecological and resource Engineering Fujian Provincial Key laboratory of Eco-Industrial Green TechnologyWuyi UniversityWuyishan 354300China Wuxi environmental Monitoring Centre Wuxi 214121China

Nitrated polycyclic aromatic hydrocarbons(NPAHs)have toxic potentials that are higher than those of their corresponding parent polycyclic aromatic hydrocarbons(PAHs)and thus have received increasing attention in recent *** this study,the occurrence,distribution,source,and human health risk assessment of 15 NPAHs and 16 PAHs were investigated in the surface water from 20 sampling sites of Lake Taihu during the dry,normal,and flood seasons of *** PAH concentrations ranged from 255 to 7298 ng/L and the NPAH concentrations ranged from not-detected(ND)to 212 ng/*** the target analytes,2-nitrofluorene(2-n Flu)was the predominant NPAH,with a detection frequency ranging from 85%to 90%and a maximum concentration of 56.2 ng/*** three-ringed and four-ringed NPAHs and PAHs comprised the majority of the detected *** terms of seasonal variation,the highest levels of the NPAHs and PAHs were in the dry season and flood season,*** ratio analysis indicated that the prime source of NPAHs was direct combustion,whereas in the case of PAHs the contribution was predominantly from a mixed pattern including pollution from unburned petroleum and petroleum *** human health risk of NPAHs and PAHs was evaluated using a lifetime carcinogenic risk assessment *** carcinogenic risk level of the targets ranged from 2.09×10^(-7)to 5.75×10^(-5)and some surface water samples posed a potential health risk.

关键词： Nitrated polycyclic aromatic hydrocarbons Surface water Occurrence Seasonal variation Human health risk assessment

Occurrence, partition behavior, source and ecological risk assessment of nitro-PAHs in the sediment and water of Taige Canal, china

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Journal of environmental Sciences 2023年第2期124卷 782-793页

作者： Jijie Kong Tao Ma Xiaoyu Cao Weidi Li Fengxiao Zhu Huan he cheng sun shaogui yang Shiyin Li Qiming Xian school of environment Nanjing Normal UniversityNanjing 210023China school of Geography Nanjing Normal UniversityNanjing 210023China The state key laboratory of pollution control and resource reuse School of the EnvironmentNanjing UniversityNanjing 210023China Fujian Provincial key laboratory of Eco-Industrial Green Technology College of Ecological and Resource EngineeringWuyi UniversityWuyishan 354300China nanjing Institute of environmental Sciences Ministry of Ecology and EnvironmentNanjing 210042China

Nitrated polycyclic aromatic hydrocarbons(NPAHs)are widespread organic pollutants that possess carcinogenic and mutagenic properties,so they may pose a risk to the environment and human *** this study,the concentrations of 15 NPAHs and 16 polycyclic aromatic hydrocarbons(PAHs)in 30 surface water samples and 26 sediment samples were measured in 2018 from the Taige Canal,one of the main rivers flowing into Taihu Lake,*** total NPAH concentrations in water and sediment ranged from 14.7 to 235 ng/L and 22.9 to 96.5 ng/g dw,respectively.9-nitrophenanthrene(nd–76.3 ng/L)was the dominant compound in surface water,while 2+3-nitrofluoranthene(1.73–18.1 ng/g dw)dominated in *** PAHs,concentration ranging from 1,097 to 2,981 ng/L and 1,089 to 4,489 ng/g dw in surface water and sediment,*** was a strong positive correlation between the log octanol-water partition coefficient(Kow)and log sediment-water partition coefficient due to hydrophobic *** fugacity fraction value increased with the decrease of log Kow,and chrysene was transferred from water into *** residual NPAHs in surface water and sediment of the Taige Canal have partial *** engine and coal combustion emissions were probably the principal sources of NPAHs in surface water and *** results of ecological risk assessment showed that some NPAHs inwater(e.g,1-nitropyrene and 6-nitrochrysene)and sediment(e.g.,2-nitrobiphenyl,5-nitroacenaphthene,9-nitrophenanthrene and 2+3-nitrofluoranthene)had moderate ecological risks,which should be of concern.

关键词： Nitrated polycyclic aromatic hydrocarbons(NPAHs) Occurrence Partition behavior Source apportionment Ecological risk assessment