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Fusion of a rice endogenous N-methylpurine DNA glycosylase to a plant adenine base transition editor ABE8e enables A-toK base editing in rice plants

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aBIOTECH 2024年第2期5卷 127-139页

作者： Yucai Li Shaoya Li Chenfei Li Chen Zhang Lei Yan Jingying Li yubing he Yan Guo Lanqin Xia Institute of Crop Sciences(ICS) Chinese Academy of Agricultural Sciences(CAAS)Beijing 100081China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Hainan Yazhou Bay Seed Laboratory/National Nanfan Research Institute(Sanya) CAASSanya 572024China

Engineering of a new type of plant base editor for simultaneous adenine transition and transversion within the editing window will greatly expand the scope and potential of base editing in directed evolution and crop ***,we isolated a rice endogenous hypoxanthine excision protein,N-methylpurine DNA glycosylase(OsMPG),and engineered two plant A-to-K(K=G or T)base editors,rAKBE01 and rAKBE02,for simultaneous adenine transition and transversion base editing in rice by fusing OsMPG or its mutant mOsMPG to a plant adenine transition base editor,*** further coupled either OsMPG or mOsMPG with a transactivation factor VP64 to generate rAKBE03 and rAKBE04,*** these four rAKBEs,at five endogenous loci in rice protoplasts,indicated that rAKBE03 and rAKBE04 enabled higher levels of A-to-G base transitions when compared to ABE8e and ***,whereas rAKBE01 only enabled A-to-C/T editing at one endogenous locus,in comparison with rAKBE02 and rAKBE03,rAKBE04 could significantly improve the A-to-C/T base transversion efficiencies by up to 6.57-and 1.75-fold in the rice protoplasts,***,although no stable lines with A-to-C transversion were induced by rAKBE01 and rAKBE04,rAKBE04 could enable simultaneous A-to-G and A-to-T transition and transversion base editing,at all the five target loci,with the efficiencies of A-to-G transition and A-to-T transversion editing ranging from 70.97 to 92.31%and 1.67 to 4.84%in rice stable lines,***,these rAKBEs enable different portfolios of editing products and,thus,now expands the potential of base editing in diverse application scenario for crop improvement.

关键词： Rice(Oryza sativa L) Rice N-methylpurine DNA glycosylase(OsMPG) A-to-K base editor(AKBE) Transactivation module VP64

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中国高技术产业创新生态系统效率测度及提升路径研究

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系统工程理论与实践 2024年第2期44卷 546-562页

作者：何郁冰张思林婷福州大学经济与管理学院福州350108

基于生态效率视角将产业创新生态系统创新流程划分为同化、生长和利用三个阶段,构建产业创新生态系统效率评价指标体系,运用Super-SBM模型和Malmquist指数法对中国15个高技术产业创新生态系统效率进行测度,并提出相应的提升路径.研究结... 详细信息

基于生态效率视角将产业创新生态系统创新流程划分为同化、生长和利用三个阶段,构建产业创新生态系统效率评价指标体系,运用Super-SBM模型和Malmquist指数法对中国15个高技术产业创新生态系统效率进行测度,并提出相应的提升路径.研究结果表明:中国高技术产业创新生态系统效率处于中等水平,总体呈持续增长趋势,产业间差异性和两极化现象显著,需进一步协同发展;三个阶段效率差异明显,利用效率最高,生长效率次之,同化效率最低,演化趋势中,同化效率增长速度最快,利用效率次之,生长效率最慢(负增长);结合产业分阶段效率的差异性发现,“双三高”效率产业尚未形成,无法发挥“创新生态效应”的引领推动作用;中国高技术产业创新生态系统效率可表征为高效率-快增长、低效率-快增长、低效率-慢增长、高效率-慢增长四种类型,不同类型产业可通过流程优化式、机制完善式和双边突破式等路径提升创新生态系统效率.

关键词：创新生态系统静态效率动态效率中国高技术产业提升路径

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联盟组合多样性对企业持续创新的影响

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科技进步与对策 2024年第5期41卷 60-69页

作者：何郁冰占加峰福州大学经济与管理学院福建福州350108

基于联盟组合多样性构成视角,探讨联盟组合中“伙伴—功能—治理”多样性对企业持续创新的影响,以及结构洞、互补资产对二者关系的调节作用,并利用2013—2017年101家中国深市创业板上市公司面板数据进行实证检验。结果表明:伙伴多样性... 详细信息

基于联盟组合多样性构成视角,探讨联盟组合中“伙伴—功能—治理”多样性对企业持续创新的影响,以及结构洞、互补资产对二者关系的调节作用,并利用2013—2017年101家中国深市创业板上市公司面板数据进行实证检验。结果表明:伙伴多样性、功能多样性和治理多样性对企业持续创新具有不同程度的影响;互补资产正向调节功能多样性与持续创新的关系,对“治理多样性—持续创新”关系发挥负向调节作用;结构洞负向调节功能多样性与持续创新的关系,对“治理多样性—持续创新”关系发挥正向调节作用。

关键词：联盟组合多样性持续创新互补资产结构洞调节作用

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A key link between jasmonic acid signaling and auxin biosynthesis

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Science China(Life Sciences) 2015年第3期58卷 311-312页

作者： he yubing ZHAO Yunde National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research Huazhong Agricultural University Section of Cell and Developmental Biology University of California San Diego

A research team led by Xiang Cheng Bin from School of Life Sciences,University of Science and Technology of China discovered a molecular basis for crosstalk between jasmonic acid（JA）and auxin,two important plant *** findings were published in a recent issue of Nature Communication[1].Auxin is the plant growth hormone that plays an essential

关键词： auxin crosstalk biosynthesis Xiang hormones Arabidopsis drought signaling abiotic roots

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Technological breakthroughs in generating transgene-free and genetically stable CRISPR-edited plants

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aBIOTECH 2020年第1期1卷 88-96页

作者： yubing he Yunde Zhao National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China Section of Cell and Developmental Biology University of California San DiegoLa JollaCA 92093-0116USA

CRISPR/Cas9 gene-editing technologies have been very effective in editing target genes in all major crop plants and offer unprecedented potentials in crop improvement.A major challenge in using CRISPR gene-editing technology for agricultural applications is that the target gene-edited crop plants need to be transgene free to maintain trait stability and to gain regulatory approval for commercial *** this article,we present various strategies for generating transgene-free and target geneedited crop *** CRISPR transgenes can be removed by genetic segregation if the crop plants are reproduced ***-assisted tracking and eliminating transgenes greatly decrease the time and labor needed for identifying the ideal transgene-free *** can be programed to undergo self-elimination when CRISPR genes and suicide genes are sequentially activated,greatly accelerating the isolation of transgene-free and target gene-edited ***-free plants can also be generated using approaches that are considered non-transgenic such as ribonucleoprotein transfection,transient expression of transgenes without DNA integration,and ***,we discuss the advantages and disadvantages of the various strategies in generating transgene-free plants and provide guidance for adopting the best strategies in editing a crop plant.

关键词： CRISPR Transgene-free Marker-assisted selection TKC Cas9 gene editing

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Synergistic roles of LAX1 and FZP in the development of rice sterile lemma

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The Crop Journal 2020年第1期8卷 16-25页

作者： Yidong Wang Shanshan Wei yubing he Lang Yan Rongchen Wang Yunde Zhao aNational Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070HubeiChina College of Life Science and Technology Huazhong Agricultural UniversityWuhan 430070HubeiChina Section of Cell and Developmental Biology University of California San DiegoLa JollaCA 92093-0116USA

Rice florets are subtended by two sterile lemmas,whose origin and biological functions have not been studied *** we demonstrate that two putative transcription factors,LAX PANICLE1(LAX1)and FRIZZY PANICLE(FZP),synergistically control the development of sterile *** LAX1 and FZP are previously known for their roles in panicle and floret *** of either LAX1 or FZP greatly reduces the number of floret *** generated new lax1 mutants(lax1-c)using CRISPR/Cas9 gene editing *** addition to the expected lax panicle phenotypes,we noticed that a significant number of spikelets of lax1-c developed elongated sterile ***,our characterization of lax1-RNAi plants also revealed sterile lemma phenotypes similar to lax1-c *** isolated a weak allele of fzp(fzp-14)in a genetic screen for lax1–1 *** fzp-14 lax1–1 double mutants completely eliminated flower ***,the isolated fzp-14 produced spikelets with elongated sterile ***,fzp-14 was haploid-insufficient in the lax1–1 background whereas fzp-14 heterozygous plants were indistinguishable from wild type *** lax1–1 fzp-14+/−also developed elongated sterile lemma as observed in lax1-c,lax1-RNAi,and fzp-14,suggesting that LAX1 and FZP synergistically control sterile lemma development.

关键词： LAX1 FZP Rice CRISPR Flower development Sterile lemma

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A reporter for noninvasively monitoring gene expression and plant transformation

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Horticulture Research 2020年第1期7卷 821-826页

作者： yubing he Tao Zhang Hui Sun Huadong Zhan Yunde Zhao State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095China National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China Section of Cell and Developmental Biology University of California San Diego9500 Gilman DriveLa JollaCA 92093-0116USA Tata Institute for Genetics and Society-UCSD La JollaCA 92093-0335USA

Reporters have been widely used to visualize gene expression,protein localization,and other cellular activities,but the commonly used reporters require special equipment,expensive chemicals,or invasive ***,we construct a new reporter RUBY that converts tyrosine to vividly red betalain,which is clearly visible to naked eyes without the need of using special equipment or chemical *** show that RUBY can be used to noninvasively monitor gene expression in ***,we show that RUBY is an effective selection marker for transformation events in both rice and *** new reporter will be especially useful for monitoring cellular activities in large crop plants such as a fruit tree under field conditions and for observing transformation and gene expression in tissue culture under sterile conditions.

关键词： transformation reporter sterile

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端对端交易-云储能市场的非合作联合博弈出清模型

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南方电网技术 2024年第1期18卷 85-93页

作者：许丹莉顾慧杰何宇斌周华锋周尚筹陈根军中国南方电网电力调度控制中心广州510663 南方电网科学研究院广州510663 南京南瑞继保电气有限公司南京211111

在“碳达峰,碳中和”的背景下,分布式可再生能源的进一步推广是电力系统低碳化的重要路径之一。为了消纳用户侧大量的分布式可再生能源,提出了端对端交易-云储能联合市场以最大化能源卖家的市场收益、最小化能源买家的用电成本。首先将... 详细信息

在“碳达峰,碳中和”的背景下,分布式可再生能源的进一步推广是电力系统低碳化的重要路径之一。为了消纳用户侧大量的分布式可再生能源,提出了端对端交易-云储能联合市场以最大化能源卖家的市场收益、最小化能源买家的用电成本。首先将能源买卖双方建模为非合作博弈的关系;其次,在考虑云储能总容量限制的情况下,求解出能源卖家的Nash均衡和能源买家的广义Nash均衡点;然后基于核求解出云储能的合作剩余的最优分配方案;最后使用爱尔兰的社区级微电网作为算例,验证了提出的端对端交易-云储能联合市场的优越性。所提出的联合市场模型能够最大化买卖双方的市场收益,实现了市场参与者效益的帕累托改进,而对比实验中的端对端交易的参与者相较于传统现货市场的参与者而言,购电成本下降了39.68%,云储能市场使得传统市场参与者的总成本降低了27.4%,因此将端对端用能交易与云储能市场耦合起来可以极大地降低用户的发电和购电成本,具有较强的实际应用价值。

关键词：端对端交易市场分布式储能云储能聚合商广义Nash均衡

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Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants

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Rice science 2019年第2期26卷 109-117,I0016,I0017页

作者： he yubing ZHU Min WANG Lihao WU Junhua WANG Qiaoyan WANG Rongchen ZHAO Yunde National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University College of Life Science and Technology Huazhong Agricultural University College of Plant Science & Technology Huazhong Agricultural University Section of Cell and Developmental Biology University of California San Diego

Elimination of the CRISPR/Cas9 constructs in edited plants is a prerequisite for assessing genetic stability, conducting phenotypic characterization, and applying for commercialization of the plants. However, removal of the CRISPR/Cas9 transgenes by genetic segregation and by backcross is laborious and time consuming. We previously reported the development of the transgene killer CRISPR(TKC) technology that uses a pair of suicide genes to trigger self-elimination of the transgenes without compromising gene editing efficiency. The TKC technology enables isolation of transgene-free CRISPR-edited plants within a single generation, greatly accelerating crop improvements. here, we presented two new TKC vectors that show great efficiency in both editing the target gene and in undergoing self-elimination of the transgenes. The new vectors replaced the CaMV35 S promoter used in our previous TKC vector with two rice promoters to drive one of the suicide genes, providing advantages over our previous TKC vector under certain conditions. The vectors reported here offered more options and flexibility to conduct gene editing experiments in rice.

关键词： genome editing suicide gene transgene killer CRISPR Cas9 transgene-free

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Plant base editing and prime editing:The current status and future perspectives

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Journal of Integrative Plant Biology 2023年第2期65卷 444-467页

作者： Jingying Li Chen Zhang yubing he Shaoya Li Lei Yan Yucai Li Ziwei Zhu Lanqin Xia Institute of Crop Sciences(ICS) Chinese Academy of Agricultural Sciences(CAAS)Beijing 100081China National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural Sciences/Hainan Yazhou Bay Seed LaboratorySanya 572024China

Precise replacement of an allele with an elite allele controlling an important agronomic trait in a predefined manner by gene editing technologies is highly desirable in crop *** editing and prime editing are two newly developed precision gene editing systems which can introduce the substitution of a single base and install the desired short indels to the target loci in the absence of double-strand breaks and donor repair templates,*** their discoveries,various strategies have been attempted to optimize both base editor(BE)and prime editor(PE)in order to improve the precise editing efficacy,specificity,and expand the targeting ***,we summarize the latest development of various BEs and PEs,as well as their applications in *** on these progresses,we recommend the appropriate BEs and PEs for both basic plant research and crop ***,we propose the perspectives for further optimization of these two *** envision that both BEs and PEs will become the routine and customized precise gene editing tools for both plant biological research and crop improvement in the near future.

关键词： base editing CRISPR/Cas plants precision genome editing prime editing

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