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Single and joint effects of pesticides and mercury on soil urease

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Journal of Environmental Sciences 2007年第2期19卷 210-216页

作者： YANG Chun-lu SUN Tie-hang he wen-xiang ZHOU Qi-xing CheN Su Key Laboratory of Environmental Engineering Shenyang University Shenyang 110044 China Key Laboratory of Terrestrial Ecological Process Institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 China

The influence of two pesticides including chlorimuron-ethyl and furadan and mercury （Hg） on urease activity in 4 soils （meadow burozem and phaeozem） was investigated. The soils were exposed to various concentrations of the two pesticides and Hg individually and simultaneously. Results showed that there was a close relationship between urease activity and organic matter content in soil. Chlorimuron-ethyl and furadan could both activate urease in the 4 soils. The maximum increment of urease activity by chlorimuronethyl was up to 14%-18%. There was almost an equal increase （up to 13%-21%） in the urease activity by furadan. On the contrary, Hg markedly inhibited soil urease activity. A logarithmic equation was used to describe the relationship （P〈0.05） between the concentration of Hg and the activity of soil urease in the 4 tested soils. Semi-effect dose （ED50） values by the stress of Hg based on the inhibition of soil urease in the 4 soils were 88, 5.5, 24 and 20 mg/kg, respectively, according to the calculation of the corresponding equations. The interactive effect of chlorimuron-ethyl or furadan with metal Hg on soil urease was mainly synergic at the highest tested concentrations.

关键词： chlorimuron-ethyl furadan mercury soil urease combined pollution

RT-nPCR Assays for Amplification and Sequencing of VP1 Genes in Human Enterovirus A–D from Clinical Specimens

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Biomedical and Environmental Sciences 2020年第11期33卷 829-838页

作者： CheN Wei wenG Yu Wei he wen xiang ZHU Ying YU Ting Ting XIE Jian Feng ZheNG Kui Cheng YAN Yan Sheng ZHANG Yong Jun ZHANG wen Chang The School of Public health Fujian Medical UniversityFuzhou 350122FujianChina Fujian Center for Disease Control and Prevention Fujian Provincial Key Laboratory of Zoonosis ResearchFuzhou 350001FujianChina

Objective To develop RT-nPCR assays for amplifying partial and complete VP1 genes of human enteroviruses(heVs)from clinical samples and to contribute to etiological surveillance of heV-related diseases.Methods A panel of RT-nPCR assays,consisting of published combined primer pairs for VP1 genes of heV A–C and in-house designed primers for heV-D,was established in this study.The sensitivity of each RT-nPCR assay was evaluated with serially diluted virus stocks of five serotypes expressed as CCID50 perμL and copies perμL,and the newly established methods were tested in clinical specimens collected in recent years.Results The sensitivity of RT-nPCR assays for amplifying partial VP1 gene of heVs was 0.1 CCID50 perμL and 10 virus copies perμL,and for the complete VP1 gene was 1 CCID50 perμL and 100 virus copies perμL,using serially-diluted virus stocks of five serotypes.As a proof-of-concept,25 serotypes were identified and complete VP1 sequences of 23 serotypes were obtained by this system among 858 clinical specimens positive for heVs during the past eight surveillance seasons.Conclusion This RT-nPCR system is capable of amplifying the partial and complete VP1 gene of heV A–D,providing rapid,sensitive,and reliable options for molecular typing and molecular epidemiology of heVs in clinical specimens.

关键词： Clinical specimens Human enterovirus A–D VP1 gene Polymerase chain reaction

Molecular Epidemiology of Coxsackievirus B1-5 Associated with HFMD in Fujian Province, China, 2011-2016

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Biomedical and Environmental Sciences 2019年第8期32卷 633-638页

作者： CheN Wei wenG Yu Wei ZHANG Yong Jun he wen xiang ZHU Ying YU Ting Ting XIE Jian Feng ZheNG Kui Cheng YAN Yan Sheng ZHANG wen Chang Fujian Centre for Disease Control and Prevention Fujian Provincial Key Laboratory of Zoonosis ResearchFuzhou 350001FujianChina School of Public health Fujian Medical UniversityFuzhou 350108FujianChina

Hand,foot and mouth disease(HFMD)is a common infectious disease that usually affects children less than 5 years of age.HFMD is caused by human enteroviruses(heVs).heVs,members of the Enterovirus genus of the Picornavi... 详细信息

关键词： Molecular Epidemiology Coxsackievirus B1-5 Associated HFMD

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基于深度学习的砂岩组分显微图像识别

科学技术与工程 2024年第9期24卷 3727-3736页

作者：任书杰胡勇何文祥高小洋万涛长江大学资源与环境学院武汉430100 中国石化中原油田物探研究院濮阳457000

识别砂岩中的石英、长石和岩屑对判断沉积环境具有重要意义,但传统的人工识别方法存在主观性强、对经验依赖程度高等问题。利用深度学习、卷积神经网络等技术构建了一种基于Faster R-CNN目标检测算法的砂岩显微组分图像识别方法,实现了... 详细信息

识别砂岩中的石英、长石和岩屑对判断沉积环境具有重要意义,但传统的人工识别方法存在主观性强、对经验依赖程度高等问题。利用深度学习、卷积神经网络等技术构建了一种基于Faster R-CNN目标检测算法的砂岩显微组分图像识别方法,实现了正交偏光下对薄片图像中石英、长石、岩屑3种组分的智能识别,3种组分平均识别准确率为89.28%。为了验证模型的可靠性,实验对比了不同算法和特征提取网络,结果表明:Faster R-CNN目标检测算法的识别效果优于YOLOv3、YOLOv4、YOLOv5s,ResNet50特征提取网络的表现效果优于VGG16。采用ResNet50特征提取网络的Faster R-CNN目标检测模型优势显著,它可以更好满足岩石薄片的识别要求,为传统的人工方法提供智能化技术方案。

关键词：深度学习卷积神经网络 Faster R-CNN 图像识别 ResNet50 岩石薄片

pH Induced Self-assembly of AIE Fluorescent Probe for Tumor Imaging

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Chinese Journal of Polymer Science 2024年第1期42卷 7-13,I0005页

作者： Jing-Yuan xiang wen-Long Zhang he-Ting Li Ya-Ning he Department of Chemical Engineering Key Laboratory of Advanced Materials(MOE)Tsinghua UniversityBejing 100084China

This work reported an AIE fluorescent probe for tumor imaging based on the p H induced self-assembly strategy. The fluorescent probe was composed of an acid-responsive soluble copolymer PEG-b-PAMA-DMMA with a maleic acid amide group and an anionic soluble aggregation-induced emission fluorogen(AIEgen) TPE-2SO_(3)^(-). The polymer could be transformed into a protonated amine-containing polymer after the hydrolysis of maleic acid amide in acidic tumor microenvironment, which would be further self-assembled with TPE-2SO_(3)^(-)to form aggregated nanoparticles. The transition of TPE-2SO_(3)^(-)from dispersed state to aggregated state led to an obvious increase in fluorescence intensity due to its AIE characteristics.

关键词： p H-responsive Self-assembly Fluorescent probe AIE Tumor imaging

Proteomic study of vitreous in proliferative diabetic retinopathy patients after treatment with aflibercept:a quantitative analysis based on 4D label-free technique

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International Journal of Ophthalmology(English edition) 2024年第4期17卷 676-685页

作者： Ting-Ting Feng xiang Gao An-Ran Liang Bo-wen Zhao Guang-Hui he Song Chen Clinical College of Ophthalmology Tianjin Medical UniversityTianjin 300070China Tianjin Key Laboratory of Ophthalmology and Visual Science Tianjin Eye InstituteTianjin Eye HospitalTianjin 300020China Key Laboratory of Ophthalmology Anyang Eye HospitalAnyang 455000Henan ProvinceChina Department of Ophthalmology Jining No.1 People’s HospitalJining 272011Shandong ProvinceChina

AIM:To identify different metabolites,proteins and related pathways to elucidate the causes of proliferative diabetic retinopathy(PDR)and resistance to anti-vascular endothelial growth factor(VEGF)drugs,and to provide biomarkers for the diagnosis and treatment of PDR.METHODS:Vitreous specimens from patients with diabetic retinopathy were collected and analyzed by Liquid Chromatography-Mass Spectrometry(LC-MS/MS)analyses based on 4D label-free technology.Statistically differentially expressed proteins(DEPs),Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway representation and protein interactions were analyzed.RESULTS:A total of 12 samples were analyzed.The proteomics results showed that a total of 58 proteins were identified as DEPs,of which 47 proteins were up-regulated and 11 proteins were down-regulated.We found that C1q and tumor necrosis factor related protein 5(C1QTNF5),Clusterin(CLU),tissue inhibitor of metal protease 1(TIMP1)and signal regulatory protein alpha(SIRPα)can all be specifically regulated after aflibercept treatment.GO functional analysis showed that some DEPs are related to changes in inflammatory regulatory pathways caused by PDR.In addition,protein-protein interaction(PPI)network evaluation revealed that TIMP1 plays a central role in neural regulation.In addition,CD47/SIRPαmay become a key target to resolve anti-VEGF drug resistance in PDR.CONCLUSION:Proteomic analysis is an approach of choice to explore the molecular mechanisms of PDR.Our data show that multiple proteins are differentially changed in PDR patients after intravitreal injection of aflibercept,among which C1QTNF5,CLU,TIMP1 and SIRPαmay become targets for future treatment of PDR and resolution of anti-VEGF resistance.

关键词： vitreous proliferative diabetic retinopathy proteome 4D label-free

Correlation and Pathway Analysis of the Carbon,Nitrogen,and Phosphorus in Soil-Microorganism-Plant with Main Quality Components of Tea(Camellia sinensis)

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Phyton-International Journal of Experimental Botany 2024年第3期93卷 487-502页

作者： Chun Mao Ji he Xuefeng wen Yangzhou xiang Jihong Feng Yingge Shu College of Agriculture Guizhou UniversityGuiyangChina School of Geography and Resources Guizhou Education UniversityGuiyangChina

The contents of carbon(C),nitrogen(N),and phosphorus(P)in soil-microorganisms-plant signiﬁcantly affect tea quality by altering the main quality components of tea,such as tea polyphenols,amino acids,and caffeine.However,few studies have quantiﬁed the effects of these factors on the main quality components of tea.The study aimed to explore the interactions of C,N,and P in soil-microorganisms-plants and the effects of these factors on the main quality components of tea by using the path analysis method.The results indicated that(1)The contents of C,N,and P in soil,microorganisms,and tea plants were highly correlated and collinear,and showed signiﬁcant correlations with the main quality components of tea.(2)Optimal regression equations were established to esti-mate tea polyphenol,amino acid,catechin,caffeine,and water extract content based on C,N,and P contents in soil,microorganisms,and tea plants(R^(2)=0.923,0.726,0.954,0.848,and 0.883,respectively).(3)Pathway analysis showed that microbial biomass phosphorus(MBP),root phosphorus,branch nitrogen,and microbial biomass carbon(MBC)were the largest direct impact factors on tea polyphenol,catechin,water extracts,amino acid,and caffeine content,respectively.Leaf carbon,root phosphorus,and leaf nitrogen were the largest indirect impact factors on tea polyphenol,catechin,and water extract content,respectively.Leaf carbon indirectly affected tea polyphenol content mainly by altering MBP content.Root phosphorus indirectly affected catechin content mainly by altering soil organic carbon content.Leaf nitrogen indirectly affected water extract content mainly by altering branch nitrogen content.The research results provide the scientiﬁc basis for reasonable fertilization in tea gardens and tea quality improvement.

关键词： Soil-microorganisms-plant system carbon nitrogen phosphorus tea quality path analysis

A 3D-printed microhemispherical shell resonator with electrostatic tuning for a Coriolis vibratory gyroscope

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Microsystems & Nanoengineering 2024年第2期10卷 25-35页

作者： Baoyin Hou Ye Zhu Chaofan he Weidong Wang Zhi Ding wen he Yong he Lufeng Che College of Information Science and Electronic Engineering Zhejiang UniversityHangzhou310027China Center for Microelectronics Shaoxing InstituteZhejiang UniversityShaoxing312035China State Key Laboratory of Fluid Power and Mechatronic Systems Zhejiang UniversityHangzhou310058China School of Mechanical Engineering Zhejiang UniversityHangzhou310058China

The emergence of microhemispherical resonant gyroscopes,which integrate the advantages of exceptional stability and long lifetime with miniaturization,has afforded new possibilities for the development of whole-angle gyroscopes.However,existing methods used for manufacturing microhemispherical resonant gyroscopes based on MEMS technology face the primary drawback of intricate and costly processing.here,we report the design,fabrication,and characterization of the first 3D-printable microhemispherical shell resonator for a Coriolis vibrating gyroscope.We remarkably achieve fabrication in just two steps bypassing the dozen or so steps required in traditional micromachining.By utilizing the intricate shaping capability and ultrahigh precision offered by projection microstereolithography,we fabricate 3D high-aspect-ratio resonant structures and controllable capacitive air gaps,both of which are extremely difficult to obtain via MEMS technology.In addition,the resonance frequency of the fabricated resonators can be tuned by electrostatic forces,and the fabricated resonators exhibit a higher quality factor in air than do typical MEMS microhemispherical resonators.This work demonstrates the feasibility of rapidly batch-manufacturing microhemispherical shell resonators,paving the way for the development of microhemispherical resonator gyroscopes for portable inertial navigation.Moreover,this particular design concept could be further applied to increase uptake of resonator tools in the MEMS community.

关键词： resonator tuning electrostatic

Low profile cavity-embedded ultra-wideband UHF array antenna with end-fire beams

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Journal of Electronic Science and Technology 2024年第2期22卷 24-35页

作者： Huan he wen-Song Wang Southwest China Institute of Electronic Technology Chengdu610036China School of Electrical and Electronic Engineering Department Nanyang Technological UniversitySingapore639798Singapore

A low-profile,vertically polarized,ultra-wideband array antenna with end-fire beams operating in an ultra-high frequency(UHF)band is developed in this paper.The array antenna consists of 1×16 log-periodic top-hat loaded monopole antenna arrays and is feasible to embed into a shallow cavity to further reduce the array height.Capacitance is introduced in the proposed antenna element to reduce profile height and the rectangular top hats are carefully designed to minimize the transverse dimension.Simulated results show that when the antenna array operates in a frequency range of 300 MHz-900 MHz,the end-fire radiation pattern achieves±45°scanning range in the horizontal plane.Then prototypes of the proposed end-fire antenna element and a uniformly spaced linear array(1×2)are fabricated and validated.The end-fire antenna array should be suitable for airborne applications where low-profile and conformal scanning phased antenna arrays with end-fire radiations are required.This design is attractive for airborne platform applications that are used to search,discover,identify,and scout the aerial target with vertically polarized beams.

关键词： End-fire radiation Low-profile Vertical polarization Log-periodic array