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内含子源性27nt-microRNA对大鼠血管平滑肌细胞表型转换的影响及其机制研究

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中国现代医学杂志 2024年第14期34卷 36-45页

作者：周炜潜罗雪兰王光耀黄凤欧和生广西中医药大学附属国际壮医医院壮瑶医药研究实验室广西南宁530201 广西中医药大学附属国际壮医医院心血管内科广西南宁530201

目的探讨内含子源性27nt-miRNA(27nt-miR)对血小板源性生长因子-BB(PDGF-BB)诱导大鼠血管平滑肌细胞(VSMCs)表型转换的影响及其分子机制。方法用27nt-miR表达慢病毒转染培养的VSMCs,观察其对PDGF-BB诱导的VSMCs表型转换的影响;将细胞... 详细信息

目的探讨内含子源性27nt-miRNA(27nt-miR)对血小板源性生长因子-BB(PDGF-BB)诱导大鼠血管平滑肌细胞(VSMCs)表型转换的影响及其分子机制。方法用27nt-miR表达慢病毒转染培养的VSMCs,观察其对PDGF-BB诱导的VSMCs表型转换的影响;将细胞分为对照组、PDGF-BB诱导组、27ntmiR组、27nt-miR-sponge组及27nt-miR NC组(27nt-miR NC组)。雷帕霉素(100 nmol/L)与MHY-1485(10μmol/L)作用于27nt-miR组、27nt-miR-sponge组及27nt-miR NC组,以验证27nt-miR是否参与mTOR与p70S6k通路的调控。用生物信息学预测27nt-miR与mTOR的靶向结合位点,用CCK-8法与EdU法分别测定VSMCs活力与增殖情况,划痕试验测定VSMCs迁移能力,实时荧光聚合酶链反应检测α-SMA、SM22α及OPN mRNA表达,Western blotting检测α-SMA、SM22α、OPN、mTOR、p-mTOR、p70S6k与pp70S6k蛋白表达。结果 CCK-8法结果示,PDGF-BB诱导组细胞增殖率较正常值高(P 0.05);27nt-miR组细胞增殖率较27nt-miR NC组低(P 0.05)。PDGFBB诱导组细胞迁移率较对照组高(P 0.05);27nt-miR组细胞迁移率较27nt-miR NC组低(P 0.05)。EdU法结果示,PDGF-BB诱导组细胞增殖率较对照组高(P 0.05);27nt-miR组细胞增殖率较27nt-miR NC组低(P 0.05)。PDGF-BB诱导组α-SMA mRNA相对表达量较对照组低(P 0.05)。27nt-miR NC组与PDGF-BB诱导组α-SMA、SM22α、OPN mRNA相对表达量比较,差异无统计学意义(P>0.05);27nt-miR组α-SMA、SM22α mRNA相对表达量较27nt-miR NC组高(P 0.05),27nt-miR-sponge组OPNm RNA相对表达量较27nt-miRNC组高(P<0.05)。PDGF-BB诱导组α-SMA、SM22α蛋白相对表达量较对照组低(P <0.05);27nt-miR NC组、PDGF-BB诱导组OPN蛋白相对表达量较27nt-miR组高(P <0.05);27nt-miR组α-SMA、SM22α蛋白相对表达量较27nt-miR NC组高(P <0.05),OPN蛋白相对表达量较27nt-miR NC组低(P <0.05);27nt-miR组p70S6k、p-p70S6k与p-mTOR蛋白相对表达量较27nt-miR NC组低(P <0.05);各组经雷帕霉素处理后,27ntmiR-sponge组与27nt-miR NC组各蛋白相对表达量比较,差异均有统计学意义(P <0.05)。27nt-miR组p70S6k、p-p70S6k、mTOR、p-mTOR蛋白相对表达量较27nt-miR NC组低(P <0.05),27nt-miR-sponge组与27nt-miR NC组p-p70S6k、mTOR、p-mTOR蛋白相对表达量比较,差异均有统计学意义(P <0.05)。各组经MHY-1485处理后,27nt-miR组p70S6k、p-p70S6k、mTOR、p-mTOR蛋白相对表达量较27ntmiR NC组低(P <0.05);27nt-miR-sponge组与27nt-miR NC组各蛋白相对表达量比较,差异均有统计学意义(P <0.05)。结论 27nt-miR可能通过靶向m TOR/p70S6k信号通路从而

关键词： 27nt-microRNA 血管平滑肌细胞 mTOR/p70S6k信号通路表型转换

A new synthetic approach to phosphatidylethanolamine

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Chinese Chemical Letters 2012年第2期23卷 154-156页

作者： Yang Song wei Yuan Yu luo wei Lu department of Chemistry East China Normal UniversityShanghai 200062China Institute of Drug Discovery and Development East China Normal UniversityShanghai 200062China

A new synthetic method for phosphatidylethanolamine head group was developed via ring-opening of cyclic dioxaphospholane 2 with sodium azide and subsequent *** advantage of this strategy included short reaction steps,... 详细信息

关键词： Phosphatidylethanolamine Synthesis

The telomere-to-telomere gap-free reference genome of wild blueberry (Vaccinium duclouxii) provides its high soluble sugar and anthocyanin accumulation

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Horticulture Research 2023年第11期10卷 141-154页

作者： Tuo Zeng Zhijiao he Jiefang he wei Lv Shixiang Huang Jiawen Li Liyong Zhu Shuang Wan Wanfei Zhou Zhengsong Yang Yatao Zhang Chong luo Jiawei he Caiyun Wang Liangsheng Wang School of Life Sciences Guizhou Normal UniversityGuiyang 550000China Institute of Alpine Economic Plant Yunnan Academy of Agricultural SciencesLijiang 674199YunnanChina School of Advanced Agricultural Sciences Peking University100871 BeijingChina National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops College of Horticulture&Forestry SciencesHuazhong Agricultural UniversityWuhan 430070China Wuhan Benagen Technology Co. LtdWuhan 430070China Key Laboratory of Plant Resources Institute of BotanyChinese Academy of SciencesBeijing 100093China china National Botanical Garden Beijing 100093China university of Chinese Academy of Sciences Beijing 100049China

Vaccinium duclouxii,endemic to southwestern china,is a berry-producing shrub or small tree belonging to the Ericaceae family,with high nutritive,medicinal,and ornamental value,abundant germplasm resources,and good edible *** addition,*** exhibits strong tolerance to adverse environmental conditions,making it a promising candidate for research and offering wide-ranging possibilities for ***,the lack of *** genome sequence has hampered its development and ***,a high-quality telomere-to-telomere genome sequence of *** was de novo assembled and *** of 12 chromosomes were assembled into gap-free single contigs,providing the highest integrity and quality assembly reported so far for *** *** genome is 573.67 Mb,which encodes 41953 protein-coding *** transcriptomics and metabolomics analyses,we have uncovered the molecular mechanisms involved in sugar and acid accumulation and anthocyanin biosynthesis in *** provides essentialmolecular information for further research on the quality of ***,the high-quality telomere-to-telomere assembly of the *** genome will provide insights into the genomic evolution of Vaccinium and support advancements in blueberry genetics and molecular breeding.

关键词： sugar offering berry

Simple inorganic base promoted polycyclic construction using mucohalic acid as a C_(3) synthon: synthesis and AIE probe application of benzo[4,5]imidazo[1,2-a]pyridines

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Organic Chemistry Frontiers 2022年第4期9卷 1127-1136页

作者： Kai Yang Zhi-Xi Chen Yong-Jun Zhou Qi Chen Shi-wei Yu Shi-he luo Zhao-Yang Wang School of Chemistry South China Normal UniversityKey Laboratory of Theoretical Chemistry of EnvironmentMinistry of EducationGuangzhou Key Laboratory of Analytical Chemistry for BiomedicineGDMPA Key Laboratory for Process Control and Quality Evaluation of Chiral PharmaceuticalsGuangzhou 510006P.R.China College of pharmacy Gannan Medical UniversityGanzhou 341000P.R.China

A transition metal-free and efficient synthesis of fused azapolycycles via a multicomponent reaction has been developed using mucohalic acid as a C_(3) *** reaction promoted by the simple inorganic base K_(2)CO_(3) gives serial C_(1)-functionalized benzo[4,5]imidazo[1,2-a]pyridine products using symmetrical and unsymmetrical substrates with satisfactory yields.

关键词： inorganic promoted synthesis

Effects of Ischemia and Anoxia on Cell Activation and Cell Cycle of Cultured Astrocytes in vitro

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Journal of Huazhong university of Science and Technology(Medical Sciences) 2006年第1期26卷 21-24页

作者：骆翔喻志源冯永东王伟 department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

To examine the effects of ischemia and anoxia on cell activation and cell cycle of astrocytes in vitro, the cell cycles and the proliferation of astrocytes in different time points after ischemia and anoxia were studied by flow cytometry and BrdU labeling and the expression of GFAP and cyclin D1 was detected by the fluorescence immunochemistry. After ischemia and anoxia in vitro, the astrocytes in S phase were significantly increased as compared with those in the normal group and the proliferating ability of the astrocytes was highest 6 h after the treatment as revealed by BrdU pulse labeling, but the astrocytes in S phase and proliferating ability were decreased after 6 h. At the early stages of ischemia and anoxia, the positive staining intensity of GFAP was increased, peaked at 6th h, while 12 h after the ischemia and anoxia, the positive staining intensity of GFAP became weak, and the expression of cyclin D1 was gradually increased after the ischemic and anoxic damage. It is concluded that astrocytes are activated to proliferate and enter new cycle events by ischemia and anoxia, and cyclin D1 is implicated in the proliferation and repair of astrocytes. The cell cycle events are closely associated with the proliferation and activation of astrocytes.

关键词： brain ischemia astrocytes cell cycle

A Localization Method of High Energy Transients for All-sky Gamma-ray Monitor

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Research in Astronomy and Astrophysics 2024年第10期 27-37页

作者： Yi Zhao Wangchen Xue Shaolin Xiong Qi luo Yuanhao Wang Jiacong Liu heng Yu Xiaoyun Zhao Yue Huang Jinyuan Liao Jianchao Sun Xiaobo Li Qibin Yi Ce Cai Shuo Xiao Shenglun Xie Chao Zheng Yanqiu Zhang Chenwei Wang Wenjun Tan Zhiwei Guo Chaoyang Li Zhenghua An Gang Chen Yanqi Du Min Gao Ke Gong Dongya Guo Jiang he Jianjian he Bing Li Gang Li Xinqiao Li Jing Liang Xiaohua Liang Yaqing Liu Xiang Ma Rui Qiao Liming Song Xinying Song Xilei Sun Jin Wang Ping Wang Xiangyang Wen Hong Wu Yanbing Xu Sheng Yang Dali Zhang Fan Zhang Hongmei Zhang Peng Zhang Shu Zhang Zhen Zhang Shijie Zheng Keke Zhang Xingbo Han Haiyan Wu Hu Tai Hao Geng Gaopeng Lu wei Xu Fanchao Lyu Hongbo Zhang Fangjun Lu Shuangnan Zhang department of Astronomy Beijing Normal University Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of Sciences university of Chinese Academy of Sciences School of Physics and Optoelectronics Xiangtan University College of Physics Hebei Normal University Guizhou Provincial Key Laboratory of Radio Astronomy and Data Processing Guizhou Normal University School of Physics and Electronic Science Guizhou Normal University Institute of Astrophysics Central China Normal University College of physics Sciences Technology Hebei University Physics and Space Science College China West Normal University School of Computing and Artificial Intelligence Southwest Jiaotong University College of Electronic and information Engineering Tongji University Innovation Academy for Microsatellites of Chinese Academy of Sciences National Space Science Center Chinese Academy of Sciences School of Earth and Space Sciences University of Science and Technology of China Electronic Information School Wuhan University Key Laboratory of Transportation Meteorology of china Meteorological Administration Nanjing Joint Institute for Atmospheric Sciences Key Laboratory of Middle Atmosphere and Global Environment Observation Institute of Atmospheric PhysicsChinese Academy of Sciences

Fast and reliable localization of high-energy transients is crucial for characterizing the burst properties and guiding the follow-up observations. Localization based on the relative counts of different detectors has been widely used for all-sky gamma-ray monitors. There are two major methods for this count distribution localization: χ2minimization method and the Bayesian method. here we propose a modified Bayesian method that could take advantage of both the accuracy of the Bayesian method and the simplicity of the χ2method. With comprehensive simulations, we find that our Bayesian method with Poisson likelihood is generally more applicable for various bursts than the χ2method, especially for weak bursts. We further proposed a location-spectrum iteration approach based on the Bayesian inference, which could alleviate the problems caused by the spectral difference between the burst and location templates. Our method is very suitable for scenarios with limited computation resources or timesensitive applications, such as in-flight localization software, and low-latency localization for rapidly follow-up observations.

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空心螺钉联合同种异体腓骨治疗股骨颈骨折的三维有限元力学研究

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中国医学工程 2024年第4期32卷 1-7页

作者：葛辉苏丽霞陈鹏汤永南罗东斌焦锋何伟广州市中西医结合医院骨科广东广州510800 广州中医药大学第一附属医院骨科广东广州510405 广州中医药大学第三附属医院骨科广东广州510378

目的探讨空心螺钉联合同种异体腓骨术式治疗股骨颈骨折的生物力学稳定性,为股骨颈骨折临床治疗新方法提供理论依据。方法采用三维有限元分析方法对空心螺钉联合同种异体腓骨术式治疗股骨颈骨折进行力学分析。基于1位45岁骨盆及股骨正常... 详细信息

目的探讨空心螺钉联合同种异体腓骨术式治疗股骨颈骨折的生物力学稳定性,为股骨颈骨折临床治疗新方法提供理论依据。方法采用三维有限元分析方法对空心螺钉联合同种异体腓骨术式治疗股骨颈骨折进行力学分析。基于1位45岁骨盆及股骨正常解剖的男性志愿者的CT数据,应用三维重建、逆向工程、有限元力学分析等技术,建立空心螺钉联合同种异体腓骨和多枚空心螺钉两种术式三维有限元模型,进行力学分析,评估两种术式内固定构型的生物力学稳定性。结果空心螺钉联合同种异体腓骨术式与多枚空心螺钉术式的股骨头颈部皮质的最大应力和位移及骨组织内部和内固定的最大应力分布位置无明显差异,但空心螺钉联合同种异体腓骨术式的股骨头颈部皮质的最大应力和位移及骨组织内部和内固定的最大应力值均比多枚空心螺钉术式小。结论空心螺钉联合同种异体腓骨术式固定股骨颈骨折的力学稳定性优于多枚空心螺钉术式,能够为股骨颈骨折愈合提供良好的力学稳定性,在股骨头发生坏死后腓骨亦可以提供力学支撑,预防股骨头塌陷,为较理想的股骨颈骨折治疗术式。

关键词：股骨颈骨折股骨头坏死生物力学三维有限元空心螺钉同种异体腓骨

Intestinal epithelial chemokine (C-C motif) ligand 7 overexpression protects against high fat diet-induced obesity and hepatic steatosis in mice

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Chinese Medical Journal 2020年第15期133卷 1805-1814页

作者： Zhi-Hong luo Meng-wei Niu Shen-Hai Gong Guang-Yan Wu Teng Wang Fang-Zhao Wang Guo-Quan wei Zhan-Ke he Yong Jiang Peng Chen department of Pathophysiology Guangdong Provincial Key Laboratory of ProteomicsSchool of Basic Medical SciencesSouthern Medical UniversityGuangzhouGuangdong 510515 China

Background:We previously found that the intestinal epithelial chemokine(C-C motif)ligand 7(CCL7)plays an important role in the development of toxin-induced acute liver *** detailed effects of intestinal epithelial CCL7 on chronic diseases;however,are still ***,we aimed to investigate the impact of intestinal epithelial CCL7 overexpression on high-fat diet(HFD)-induced obesity and steatohepatitis in ***:Intestinal epithelial CCL7 overexpression(CCL7tgIEC)mice and their wild-type(WT)littermates were fed with normal chow or HFD for 16 weeks to induce obesity and non-alcoholic fatty liver *** weight gain,as well as adipose tissue index were *** injury was monitored by histological analysis and real time polymerase chain *** microbial composition was analyzed by 16S rRNA gene ***:We found that the CCL7tgIEC mice on a HFD had markedly decreased weight gain(8.9 vs.17.0 g,Phepatic lipid accumulation and lower expression of inflammatory factors than WT mice.16S rRNA gene sequencing demonstrated that CCL7 overexpression in intestinal epithelial cells improved HFD-induced gut microbial ***:Our study revealed that CCL7 overexpression in the intestinal epithelium protects mice against the progression of diet-induced obesity,hepatic steatosis,and enteric dysbiosis.

关键词： Chemokine(C-C motif)ligand 7 Gut microbiota High-fat diet Obesity Steatohepatitis

Dithienylethene metallodendrimers with high photochromic efficiency

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Chinese Chemical Letters 2022年第3期33卷 1613-1618页

作者： Yuxuan Wang Qifeng Zhou Xiaoxiao he Ying Zhang Hongwei Tan Jianhua Xu Cuihong Wang wei Wang Xiping luo Jinquan Chen Lin Xu Shanghai Key Laboratory of Green Chemistry and Chemical Processes School of Chemistry and Molecular EngineeringEast China Normal UniversityShanghai 200062China State Key Laboratory of Precision Spectroscopy East China Normal UniversityShanghai 200241China Zhejiang Provincial Key Laboratory of Chemical Utilization of Forestry Biomass Department of ChemistryZhejiang A&F UniversityHangzhou 311300China College of Chemistry Beijing Normal UniversityBeijing 100050China

It has been challenging to achieve multi-photochromic systems without affecting the individual photoswitching properties of the constituent units. herein, we present the design and synthesis of a new family of platinum-acetylide dendrimers containing up to twenty-one photochromic dithienylethene(DTE)units that exhibit both high photochromic efficiency and individual switching properties. Upon irradiation with ultraviolet(UV) and visible(vis) light, the resultant metallodendrimers display high conversion yield and good fatigue resistance. More interestingly, cyclization-cycloreversion kinetics revealed that the photochromic property of each DTE unit in these metallodendrimers is unaffected by its neighbor and the full ring-closure of up to twenty-one DTE units in one single dendrimer has been achieved.

关键词： Photochromic compounds Stimulus response Dendrimers Platinum-acetylide complexes Dithienylethene