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Enhanced hyperplasia in muscles of transgenic zebrafish expressing Follistatin1

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Science China(Life Sciences) 2011年第2期54卷 159-165页

作者： LI Xi NIE Fen YIN Zhan he jiangyan Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China

Myostatin is a member of the transforming growth factor-β(TGF-β) super-family and functions as a negative regulator of muscle *** of the specific receptor,Activin receptor IIB(Act RIIB),with myostatin or other related TGF-β members,could be inhibited by the activin-binding protein follistatin(Fst) in *** Fst in mouse skeletal muscle leads to muscle hypertrophy and *** determine if Fst has similar roles in fish,we generated transgenic zebrafish expressing high levels of zebrafish Fst1 using the promoter of the zebrafish skeletal muscle-specific gene,myosin,light polypeptide 2,skeletal muscle(Mylz2).Independent transgenic zebrafish lines exhibited elevated expression levels of myogenic regulatory genes MyoD and Pax7 in muscle *** Fst1 overexpressing transgenic zebrafish exhibited a slight body weight *** high level of Fst1 expression dramatically increased myofiber numbers in skeletal muscle,without significantly changing the fiber *** findings suggest that Fst1 overexpression can promote zebrafish muscle growth by enhancing myofiber hyperplasia.

关键词： Follistatin1 myostatin muscle growth transgenic zebrafish

Neuroendocrine regulation of somatic growth in fishes

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Science China(Life Sciences) 2015年第2期58卷 137-147页

作者： DAI XiangYan ZHANG Wei ZHUO ZiJian he jiangyan YIN Zhan Key Laboratory of Aquatic Biodiversity and Conservation of the Chinese Academy of Sciences Institute of Hydrobiology Chinese Academy of Sciences University of Chinese Academy of Sciences

Growth is a polygenic trait that is under the influence of multiple physiological pathways regulating energy metabolism and muscle *** the possible growth-regulating pathways in vertebrates,components of the somatotropic axis are thought to have the greatest *** is growing body of literature focusing on the somatotropic axis and its role regulating growth in *** includes research into growth hormone,upstream hypothalamic hormones,insulin-like growth factors,and downstream signaling *** of these signals have both somatic effects stimulating the growth of tissues and metabolic effects that play a role in nutrient *** of other endocrine axes exhibit profound effects on the function of the somatotropic axis in *** this review we highlight recent advances in our understanding of the teleost fish endocrine somatotropic axis,including emerging research using genetic modified *** studies have revealed new aspects and challenges associated with regulation of the important steps of somatic growth.

关键词： somatic growth endocrine regulation fish

A color-based FHGC approach facilities DNA-based clinical molecular diagnostics

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Genes & Diseases 2023年第4期10卷 1197-1199页

作者： Ruyi Zhang Dongyang Deng Min Ren jiangyan he Fang Chen Shujuan Yan Obstetrics Department First Affiliated Hospital of Guizhou University of Traditional Chinese MedicineGuiyangGuizhou 550001China Center for Eugenics Research First Affiliated Hospital of Guizhou University of Traditional Chinese MedicineGuiyangGuizhou 550001China Department of Clinical Laboratory First Affiliated Hospital of Guizhou University of Traditional Chinese MedicineGuiyangGuizhou 550001China Department of Prenatal Diagnostic Center Guangzhou Women and Children’s Medical CentreGuangzhou Medical UniversityGuangzhouGuangdong 511436China Department of Prenatal Diagnosis Center Guizhou Provincial People’s HospitalThe Affiliated Hospital of Guizhou UniversityGuiyangGuizhou 550002China

The flow-through hybridization and gene chip(FHGC)was developed to used in clinical molecular diagnostics for thalassemia,human papillomavirus(HPV),and other *** could improve hybridization efficiency and reduce hands-on time,thus improving precision,repro-ducibility,and *** genotypes can be detected simultaneously without incurring high costs.^(1)During the experiment,the polymerase chain reaction(PCR)product or buffer forced through the membrane matrix,PCR products are apprehended by a probe attached to the membrane.

关键词： clinical attached diagnostics

Viscosity Approximation Methods for Common Fixed Points of a Finite Family of Quasi-Nonexpansive Mappings in Real Hilbert Spaces

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Journal of Mathematical Research with Applications 2016年第3期36卷 351-358页

作者： jiangyan he Haiyun ZHOU Xiaoyan HUO Department of Mathematics Shijiazhuang Mechanical Engineering College

In this paper, we present two parallel algorithms to compute common fixed points of a finite family of quasi-nonexpansive mappings in real Hilbert spaces, which improve and generalize some known results in this direct... 详细信息

关键词： quasi-nonexpansive mappings fixed point variational inequality parallel algorithms

LRH-1 senses signaling from phosphatidylcholine to regulate the expansion growth of digestive organs via synergy with Wnt/β-catenin signaling in zebrafish

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Journal of Genetics and Genomics 2017年第6期44卷 307-317页

作者： Gang Zhai Jia Song Tingting Shu Junjun Yan Xia Jin jiangyan he Zhan Yin State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of Sciences University of the Chinese Academy of Sciences

Liver receptor homolog-1(LRH-1)is an orphan nuclear receptor that is critical for the growth and proliferation of cancer cells and other biological processes,including lipid transportation and metabolism,sexual determination and ***,because homozygous lrh-1mice die in utero,the regulatory mechanisms involved in embryonic development mediated by this receptor are poorly *** the present study,we performed transcription activator-like effector nuclease(TALEN)-mediated loss-of-function assays,taking advantage of zebrafish external fertilization,to investigate the function of *** digestive organs were affected by lrh-1 depletion as a result of cellcycle arrest(at the checkpoint of G1 to S phase),but not cell *** analysis revealed that LRH-1 augments the transcriptional activity of b-catenin 1 and 2 via physical *** the specific ligand(s)sensed by LRH-1 during organogenesis revealed that phosphatidylcholine(PC),a potential ligand,is the upstream target of LRH-1 during endoderm *** data provide evidence for the crosstalk between the PC/LRH-1 and Wnt/β-catenin signaling pathways during the expansion growth of endoderm organs.

关键词： Zebrafish LRH-1 Phosphatidylcholine Wnt/b-catenin signaling Development

Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity

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Journal of Molecular Cell Biology 2020年第4期12卷 291-304页

作者： Chuang Shi Yao Lu Gang Zhai Jianfei Huang Guohui Shang Qiyong Lou Dongliang Li Xia Jin jiangyan he Zhenyu Du Jianfang Gui Zhan Yin State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China School of Life Sciences East China Normal UniversityShanghai 200241China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100864China

The endocrine regulatory roles of the hypothalamic-pituitary-adrenocortical axis on anxiety-like behavior and metabolic status have been found throughout animal ***,the precise effects of the balancing adrenal corticosteroid biosynthesis under the influence of adrenocorticotrophic hormone(ACTH),a pro-opiomelanocortin(POMC)-derived peptide,on animal energy expenditure and somatic growth remain *** has also been identified as one of the candidate loci for polycystic ovary syndrome,which features hyperandrogenism and some prevalence of obesity in *** we show that zebrafish lacking functional POMCa exhibit similar phenotypes of stress response and body weight gain but not obesity as observed in mammalian *** contrast with the impaired anorexigenic signaling cascade of melanocyte-stimulating hormones and leptin,which are responsible for their obesity-prone weight gain observed in various pome mutant mammals,analyses with our pomca mutant series indicate that ACTH is the key regulator for the phenotype with enhanced somatic growth without obesity in pomca-deficient *** associated with hyperandrogenism has been observed in the pomca-deficient zebrafish,with enhanced activation of mammalian target of rapamycin complex 1;reutilization of amino acids and fatty acid^-oxidation are observed in the muscle tissue of the pomca-deficient *** reducing hyperandrogenism by crossing our pomca mutant fish with a cy p l 7a 1-deficient background,the phenotype of enhanced somatic growth in pomca-deficient fish was no longer ***,our work also demonstrated that the role of POMCa in stress response seems to be conserved in vertebrates,whereas its effect on adipostasis is unique to teleosts.

关键词： anabolic metabolism HPI axis pomca somatic growth stress response

Genomic polymorphisms at the crhr2 locus improve feed conversion efficiency through alleviation of hypothalamus-pituitary-interrenal axis activity in gibel carp(Carassius gibelio)

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Science China(Life Sciences) 2022年第1期65卷 206-214页

作者： Chuang Shi Qiyong Lou Beide Fu Junyan Jin Jianfei Huang Yao Lu Xia Jin jiangyan he Gang Zhai Shouqi Xie Jingou Tong Jianfang Gui Zhan Yin State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100864China

Improvement in fish feed conversion efficiency(FCE)is beneficial for sustaining global food fish ***,we show that a set of polymorphisms at locus of the corticotropin releasing hormone receptor 2(crhr2),which is involved in hypothalamuspituitary-interrenal(HPI)axis signaling,is associated with improved FCE in farmed allogynogenetic gibel carp strain CAS Ⅲ compared with that in the wild gibel carp strain Dongting(DT).This set of polymorphisms downregulates the expression levels of crhr2 mRNA in the brain and pituitary tissues in gibel carp strain CAS Ⅲ compared with those in strain ***,compromised HPI axis signaling is observed in gibel carp strain CAS Ⅲ,such as decreased α-melanocyte stimulating hormone protein levels,plasma cortisol content,and stress ***,enhanced activation of protein kinase B/mammalian target of rapamycin complex 1 signaling observed in the muscle tissue of strain CAS Ⅲ in comparison to that in strain DT indicated elevated anabolic metabolism in strain CAS Ⅲ.Thus,these studies demonstrate that the genetic markers associated with compromised HPI axis signaling,such as crhr2,are potentially useful for genetic selection toward improvement in farmed fish growth and FCE,which would reduce fishmeal consumption and thereby indirectly facilitate sustainable fisheries.

关键词： feed conversion efficiency crhr2 stress responses endocrine regulation genetic marker for fish breeding

Successful Production of an All-Female Common Carp (Cyprinus carpio L.) Population Using cyp17a1-Deficient Neomale Carp

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Engineering 2022年第1期8卷 181-189页

作者： Gang Zhai Tingting Shu Kuangxin Chen Qiyong Lou Jingyi Jia Jianfei Huang Chuang Shi Xia Jin jiangyan he Donghuo Jiang Xueqiao Qian Wei Hu Zhan Yin State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100101China College of Fisheries Huazhong Agricultural UniversityWuhan 430070China HAID Research Institute Guangdong HAID Group Co.Ltd.Guangzhou 511400China The Innovative Academy for Seed Design Chinese Academy of SciencesBeijing 100101China

Due to sexual dimorphism in the growth of certain cultured fish species,the production of monosex fishes is desirable for the aquaculture ***,the most widely practiced technique available for the mass production of monosex fish populations is sex steroid-induced sex ***,a novel strategy for the successful production of all-female(AF)common carp(Cyprinus carpio L.),to take advantage of the sexual dimorphism in growth documented in this species,has been developed using genetic engineering via single gene-targeting manipulation without any exogenous hormone *** and female heterozygous cyp17a1-deficient common carp were first obtained using the clustered regularly interspaced short palindromic repeats/CRISPR-associated endonuclease 9(CRISPR/Cas9)*** all-male phenotype for homozygous cyp17a1-deficient carp,regardless of the individuals’sexdetermination genotypes(XY or XX),has been observed.A male-specific DNA marker newly identified in our laboratory was used to screen the neomale carp population with the XX genotype from the cyp17a1-deficient *** neomale carp develop a normal testis structure with normal spermatogenesis and sperm *** neomale common carp were then mated with wild-type(WT)females(cyp17a1^(+/+)XX genotype)using artificial *** the AF offspring sample fish from the neomale-WT female mating were confirmed as having the cyp17a^(1+/-)XX genotype,and normal development of gonads to ovaries was observed in 100.00%of this group at eight months post-fertilization(mpf).A total of 1000 carp fingerlings,500 from the WT male and female and 500 from the neomale and WT female mating,were mixed and reared in the same *** average body weight of cyp17a1^(+/-)XX females was higher by 6.60%(8 mpf)and 32.66%(12 mpf)than that of the control common *** study demonstrates the first successful production of a monosex teleost population with the advantages of sexual dimorphism in growth using gen

关键词： Common carp Sexual dimorphism Growth cyp17a1 Sex steroids All-female population

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无人直升机地面监控电子地图的设计与实现

北京航空航天大学学报 2011年第5期37卷 615-618页

作者：何江艳赵琦北京航空航天大学电子信息工程学院北京100191

为满足共轴式无人直升机飞行实时监测的要求,在Windows平台下,设计了地面监控系统中的电子地图导航模块,配合系统的显示模块、任务规划执行模块和通信模块,实现了飞行参数获取、航迹规划、飞行航迹实时显示等功能.介绍了电子导航地图的... 详细信息

为满足共轴式无人直升机飞行实时监测的要求,在Windows平台下,设计了地面监控系统中的电子地图导航模块,配合系统的显示模块、任务规划执行模块和通信模块,实现了飞行参数获取、航迹规划、飞行航迹实时显示等功能.介绍了电子导航地图的功能,分析MapInfo组件的功能和特点,阐述在Visual C++环境下基于MapInfo组件开发共轴式无人直升机地面监控系统电子导航地图的方法.通过引入MapX控件和应用MFC(Microsoft FoundationClasses),实现系统对共轴式无人直升机的监控和电子导航地图的经纬度范围的自适应调整,在电子导航地图应用上提出了一套简单全面的实践方案,同时给出了方案的实现步骤和部分关键代码.

关键词：无人机导航图形用户界面软件设计