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Cloud-based smartphone-assisted chemiluminescent assay for rapid screening of electroactive bacteria

Science China(Technological Sciences) 2023年第3期66卷 743-750页

作者： WEN JunLin he daigui LUO SongQing ZHOU ShunGui YUAN Yong Guangdong Key Laboratory of Environmental Catalysis and health Risk Control School of Environmental Science and EngineeringInstitute of Environmental Health and Pollution ControlGuangdong University of TechnologyGuangzhou 510006China College of Artificial Intelligence Guangdong Mechanical&Electrical PolytechnicGuangzhou 510550China Fujian Provincial Key Laboratory of Soil Environmental health and Regulation College of Resources and EnvironmentFujian Agriculture and Forestry UniversityFuzhou 350002China

Electroactive bacteria(EAB)can transfer electrons to exocellular solid acceptors and have widespread applications in the fields of pollutant degradation,biosynthesis,and hydrogen *** EAB screening methods can precisely measure the bacterial extracellular electron transfer ability,but they suffer from time-consuming and labor-intensive *** chemiluminescence technique is effective for the rapid detection of hemoglobin but has not been applied for EAB ***,we utilized a chemiluminescent assay to identify EAB through a multiheme c-type cytochrome(c-Cyt)triggered chemiluminescent *** multiheme c-Cyts-triggered chemiluminescence was conveniently determined by taking photographs with commercial smartphones and cloud-based *** acquired image signal shows a significant and linear relationship with the bacterial *** results of the chemiluminescence intensity triggered by different bacterial species with the proposed method are strongly associated with those of the microplate reading method(Pearson’s r of 0.9795;Phermore,this cloud-based and smartphone-assisted chemiluminescent assay is instrument-free and easy to operate,and results can be obtained within 2 *** findings demonstrate that the proposed method can effectively detect ***,it will be an alternative approach for EAB screening and can have promising applications in microbiology,environmental science,and bioenergy.

关键词： electroactive bacteria c-type cytochrome chemiluminescence cloud computing smartphone

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论劳动联合体的形成及其协作功能

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南京社会科学 2011年第5期 151-156页

作者：贺代贵湘潭大学商学院长沙市社科院副研究员湖南湘潭411105

劳动联合体是指由知识资源为纽带组织起来的团体性的集体决策组织或机构。劳动联合体源自要素产权与劳动联合产权的结合并对单一劳动产权的扬弃。劳动联合体的劳动协作促使企业治理模式不断创新,也使得企业合作剩余分配共享成为现实。

关键词：劳动联合体形成协作功能

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长株潭一体化呼唤文化融合

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城市 2007年第4期 70-73页

作者：胡艺华贺代贵湖南女子职业大学办公室长沙市社科联

如何推进长株潭一体化．是关乎湖南发展、中部崛起的一个战略问题。近年来人们大多是从经济、行政等方面来思考对策．但很少有人关注文化在长株潭一体化中的地位和作用。随着知识经济的发展．文化日益成为经济发展的重要动力和重要资源... 详细信息

如何推进长株潭一体化．是关乎湖南发展、中部崛起的一个战略问题。近年来人们大多是从经济、行政等方面来思考对策．但很少有人关注文化在长株潭一体化中的地位和作用。随着知识经济的发展．文化日益成为经济发展的重要动力和重要资源．从一定意义上说．文化的发展水平能够体现一个区域乃至一个国家的发展水平。因此在推进长株潭一体化的进程中．必须认真做好文化融合这篇文章。

关键词：文化融合一体化长株潭经济发展知识经济

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基于“前孵化器”的高职创新创业教育实践体系研究

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创新创业理论研究与实践 2024年第1期7卷 192-194,198页

作者：李志斌徐娅丹何带桂广东机电职业技术学院广东广州510440

创新创业教育是当前我国职业教育重要的人才培养方式,也是高职院校内涵式发展与教育改革的关键领域。“前孵化器”作为一种新的项目孵化模式,为学生提供产品系统和相关技术的支持,有效降低大学生创业者的创业成本压力与市场风险。该文... 详细信息

创新创业教育是当前我国职业教育重要的人才培养方式,也是高职院校内涵式发展与教育改革的关键领域。“前孵化器”作为一种新的项目孵化模式,为学生提供产品系统和相关技术的支持,有效降低大学生创业者的创业成本压力与市场风险。该文在高职院校创新创业教育体系引入“前孵化器”,构建基于“前孵化器”、以“政产教研赛”为形式的高职创新创业教育实践体系,通过“点+线+面+体”技术服务模式为创新创业教育提供实践方案。

关键词： “前孵化器” 高职创新创业职业教育产教融合教育实践

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棉花种衣剂对防治棉苗立枯病试验研究

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云南农业大学学报 2000年第3期15卷 234-236页

作者：周光胜孙晓阳邓先明朱玉香秦森荣何平何朝模何代贵四川南部县植保公司南部637300 西南农业大学重庆400716 重庆种衣剂厂重庆402160

棉苗立枯病是棉花苗期的毁灭性病害 ,棉种包衣防治棉苗立枯病是防治此病的新途径。据此 ,进行了不同新剂型、种衣剂复配防治棉苗立枯病的试验 ,结果以含PCNB(五氯硝基苯 )的克·福·多效果最佳 ,在人工接菌条件下 ,对立枯病的保苗效果达... 详细信息

棉苗立枯病是棉花苗期的毁灭性病害 ,棉种包衣防治棉苗立枯病是防治此病的新途径。据此 ,进行了不同新剂型、种衣剂复配防治棉苗立枯病的试验 ,结果以含PCNB(五氯硝基苯 )的克·福·多效果最佳 ,在人工接菌条件下 ,对立枯病的保苗效果达 78 8% ,与标准对照卫福牌种衣剂 (保苗效果 81 9% )相当 ,且可能有促生作用。

关键词：棉花种衣剂立枯病药效试验棉苗杀菌剂

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Prunus sunhangii: A new species of Prunus from central China

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Plant Diversity 2019年第1期41卷 19-25页

作者： Xiaoshuang Zhang Zhilin Jiang Ziyoviddin Yusupov Menghua Zhang daigui Zhang Komiljon Tojibaev Ying Meng Tao Deng Key Laboratory of Plant Resources Conservation and Utilization Jishou University Puer University Institute of Comparative Study of Traditional Materia Medica Institutes of Integrative Medicine Fudan University Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of Botany Chinese Academy of Sciences Central herbarium of Uzbekistan Institute of Botany Academy Sciences of Uzbekistan

A new species of Rosaceae from Central China, Prunus sunhangii D. G. Zhang & T. Deng, sp. nov., is described and illustrated. The new species is placed in Prunus subgenus Cerasus by flower and fruit characteristics. It is most similar to Prunus cerasoides, but differs by having longitudinally 2-lobed apical petals, an acuminate leaf apex, 17—25 stamens, white petals, dark black drupes, brown hypanthium, and different phenology. The phylogenetic placement of this species was assessed based on morphological and molecular data. Molecular analysis(cp DNA + ITS) corroborated its placement in subgenus Cerasus,specifically Prunus section Serrula.

关键词： Phylogenetic analyses Taxonomy Cerasus Section Serrula

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Microvesicles(MIVs)secreted from adipose-derived stem cells(ADSCs)contain multiple microRNAs and promote the migration and invasion of endothelial cells

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Genes & Diseases 2020年第2期7卷 225-234页

作者： Bo Huang Lin-Feng Huang Ling Zhao Zongyue Zeng Xi Wang daigui Cao Lijuan Yang Zhenyu Ye Xian Chen Bin Liu Tong-Chuan he Xiaozhong Wang Ministry of Education Key Laboratory of Diagnostic Medicine and School of Laboratory Medicine and The Affiliated Hospitals of Chongqing Medical UniversityChongqing Medical UniversityChongqing400016China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL60637USA Department of Clinical Laboratory Medicine The Second Affiliated Hospital of Nanchang UniversityNanchang330006China Department of Clinical Laboratory Medicine Jiangxi Maternal and Child Health HospitalNanchang330006China Department of Orthopaedic Surgery Chongqing General Hospital Affiliated with the University of Chinese Academy of SciencesChongqing400013China Department of Obstetrics and Gynecology The First Hospital of Lanzhou UniversityLanzhou730030China Department of General Surgery The Second Affiliated Hospital of Soochow UniversitySuzhou215004China Department of Clinical Laboratory Medicine The Affiliated Hospital of Qingdao UniversityQingdao266061China Department of Biology School of Life SciencesSouthwest UniversityChongqing400715China

Extracellular vesicles(EVs)such as microvesicles(MIVs)play an important role in intercellular *** are small membrane vesicles sized 100e1000 nm in diameter that are released by many types of cells,such as mesenchymal stem cells(MSCs),tumor cells and adipose-derived stem cells(ADSC).As EVs can carry out autocrine and paracrine functions by controlling multiple cell processes,it is conceivable that EVs can be used as delivery vehicles for treating several clinical conditions,such as to improve cardiac angiogenesis after myocardial infarction(MI).here,we seek to investigate whether ADSC-derived MIVs contain microRNAs that regulate angiogenesis and affect cell migration of endothelial *** first characterized the ADSC-derived MIVs and found that the MIVs had a size range of 100 e300 nm,and expressed the MIV marker protein *** then analyzed the microRNAs in ADSCs and ADSC-derived MIVs and demonstrated that ADSC-derived MIVs selectively released a panel of microRNAs,several of which were related to angiogenesis,including two members of the let-7 ***,we demonstrated that ADSC-derived MIVs promoted the cell migration and invasion of the HUVEC endothelial *** PKH26-labeled ADSC-derived MIVs were effectively uptaken into the cytoplasm of HUVEC ***,our results demonstrate that the ADSC-derived MIVs can promote migration and invasion abilities of endothelial cells,suggesting pro-angiogenetic *** studies should focus on investigating the roles and mechanisms through which ADSC-derived MIVs regulate angiogenesis.

关键词： Adipose-derived stem cells(ADSCs) Angiogenesis Let-7 microRNA Microvesicle(MIV)

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农业水价的困境摆脱与当下因应

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改革 2016年第9期 107-114页

作者：李然田代贵重庆社会科学院农村发展研究所重庆400020 重庆社会科学院区域发展研究所重庆400020

水价改革是提高农业用水效率、增强农民节水意识、改善灌溉工程运行质量的重要举措。农村税费改革以来,农业水价改革陷入"两难"境地:一方面,若维持现有水价,则因水价水平低于供水成本而导致农田水利设施管养经费严重不足,工程难以正常运... 详细信息

水价改革是提高农业用水效率、增强农民节水意识、改善灌溉工程运行质量的重要举措。农村税费改革以来,农业水价改革陷入"两难"境地:一方面,若维持现有水价,则因水价水平低于供水成本而导致农田水利设施管养经费严重不足,工程难以正常运行,更难以约束农民的用水行为;另一方面,若提高水价,则导致农民负担加重、农业减产。因此,有必要从农业水价改革的历史背景、价格形成机制、实践效果、路径选择与优化四个维度对相关文献进行梳理以揭示问题的症结。结果表明,现有研究需在研究对象拓展、理论框架构建、研究方法和视角完善等三个方面加以突破。

关键词：农业水价农田水利工程 “三农”问题

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交易平台、运行体系与农村建设用地资本化

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改革 2014年第10期 128-135页

作者：陈悦田代贵重庆社会科学院农村发展研究所重庆400020 重庆社会科学院区域经济研究所重庆400020

建立城乡统一的建设用地市场,核心是把长期行政配置的农村建设用地纳入市场化配置,基本思路是推进农村建设用地资本化,主要路径是廓清市场交易客体,赋予村社集体及农民个人产权主体地位,建立市场交易平台,建立和完善配套制度。

关键词：农村建设用地城乡用地市场“三农”问题

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Transcriptomic landscape regulated by the 14 types of bone morphogenetic proteins(BMPs)in lineage commitment and differentiation of mesenchymal stem cells(MSCs)

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Genes & Diseases 2019年第3期6卷 258-275页

作者： Linghuan Zhang Qing Luo Yi Shu Zongyue Zeng Bo Huang Yixiao Feng Bo Zhang Xi Wang Yan Lei Zhenyu Ye Ling Zhao daigui Cao Lijuan Yang Xian Chen Bin Liu William Wagstaff Russell R*Reid Hue H*Luu Rex C*Haydon Michael J*Lee Jennifer Moriatis Wolf Zhou Fu Tong-Chuan he Quan Kang Stem Cell Biology and Therapy Laboratory Ministry of Education Key Laboratory of Child Development and DisordersThe Children’s Hospital of Chongqing Medical UniversityChongqing 400014China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA c Ministry of Education Key Laboratory of Diagnostic Medicine School of Laboratory MedicineChicagoIL 60637USA d The Affiliated Hospitals of Chongqing Medical University Chongqing 400016China e Department of Clinical Laboratory Medicine The Second Affiliated Hospital of Nanchang UniversityNanchang 330006China f Key Laboratory of Orthopaedic Surgery of Gansu Province Departments of Orthopaedic Surgery and Obstetrics and GynecologyThe First and Second Hospitals of Lanzhou UniversityLanzhou 730030China g Department of General Surgery The Second Affiliated Hospital of Soochow UniversitySuzhou 215004China Department of Clinical Laboratory Medicine The Affiliated Hospital of Qingdao UniversityQingdao 266061China Department of Biology School of Life SciencesSouthwest UniversityChongqing 400715China Department of Surgery Section of Plastic SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA

Mesenchymal stem cells(MSCs)are ubiquitously-existing multipotent progenitors that can self-renew and differentiate into multiple lineages including osteocytes,chondrocytes,adipocytes,tenocytes and *** represent one of the most commonly-used adult progenitors and serve as excellent progenitor cell models for investigating lineagespecific differentiation regulated by various cellular signaling pathways,such as bone morphogenetic proteins(BMPs).As members of TGFb superfamily,BMPs play diverse and important roles in development and adult *** least 14 BMPs have been identified in *** BMPs exert distinct but overlapping biological *** a comprehensive analysis of 14 BMPs in MSCs,we demonstrated that BMP9 is one of the most potent BMPs in inducing osteogenic differentiation of ***,a global mechanistic view of BMP signaling in regulating the proliferation and differentiation of MSCs remains to be fully ***,we conducted a comprehensive transcriptomic profiling in the MSCs stimulated by 14 types of *** clustering analysis classifies 14 BMPs into three subclusters:an osteo/chondrogenic/adipogenic cluster,a tenogenic cluster,and BMP3 *** also demonstrate that six BMPs(e.g.,BMP2,BMP3,BMP4,BMP7,BMP8,and BMP9)can induce ISmads effectively,while BMP2,BMP3,BMP4,BMP7,and BMP11 up-regulate Smad-independent MAP kinase ***,we show that many BMPs can upregulate the expression of the signal mediators of Wnt,Notch and PI3K/AKT/mTOR *** the reported transcriptomic changes need to be further validated,our expression profiling represents the first-of-its-kind to interrogate a comprehensive transcriptomic landscape regulated by the 14 types of BMPs in MSCs.

关键词： Bone morphogenetic proteins(BMPs) MAP kinase signaling Mesenchymal stem cells Notch signaling PI3K/AKT/mTOR pathway Smad signaling TGFb superfamily Wnt signaling

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